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RBC Lysis Buffer (10X)

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RUO
Other Names
Red blood cell lysis buffer
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420301 100 mL 65 CHF
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420302 500 mL 206 CHF
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Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  2. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  3. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  4. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  5. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  6. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  7. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  8. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  9. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  10. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  11. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  12. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  13. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  14. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  15. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  16. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  17. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  18. Melendez E, et al. 2022. Development. 149: . PubMed
  19. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  20. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  21. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  22. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  23. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  24. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  25. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  26. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  27. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  28. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  29. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  30. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  31. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  32. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  33. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  34. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  35. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  36. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  37. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  38. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  39. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  40. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  41. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  42. Feng J, et al. 2022. Immunity. 55:405. PubMed
  43. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  44. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  45. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  46. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  47. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  48. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  49. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  50. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  51. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  52. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  53. Le DT, et al. 2023. iScience. 26:106059. PubMed
  54. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  55. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  56. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  57. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  58. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  59. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  60. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  61. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  62. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  63. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  64. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  65. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  66. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  67. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  68. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  69. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  70. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  71. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  72. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  73. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  74. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  75. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  76. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  77. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  78. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  79. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  80. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  81. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  82. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  83. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  84. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  85. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  86. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  87. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  88. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  89. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  90. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  91. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  92. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  93. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  94. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  95. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  96. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  97. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  98. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  99. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  100. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  101. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  102. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  103. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  104. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  105. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  106. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  107. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  108. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  109. Meng S, et al. 2022. Exp Anim. . PubMed
  110. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  111. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  112. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  113. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  114. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  115. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  116. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  117. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  118. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  119. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  120. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  121. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  122. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  123. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  124. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  125. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  126. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  127. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  128. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  129. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  130. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  131. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  132. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  133. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  134. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  135. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  136. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  137. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  138. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  139. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  140. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  141. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  142. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  143. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  144. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  145. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  146. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  147. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  148. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  149. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  150. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  151. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  152. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  153. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  154. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  155. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  156. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  157. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  158. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  159. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  160. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  161. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  162. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  163. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  164. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  165. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  166. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  167. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  168. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  169. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  170. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  171. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  172. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  173. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  174. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  175. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  176. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  177. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  178. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  179. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  180. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  181. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  182. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  183. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  184. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  185. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  186. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  187. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  188. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  189. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  190. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  191. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  192. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  193. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  194. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  195. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  196. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  197. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  198. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  199. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  200. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  201. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  202. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  203. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  204. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  205. Sci T 2013. Toxicol Sci. 136:72. PubMed
  206. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  207. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  208. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  209. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  210. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  211. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  212. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  213. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  214. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  215. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  216. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  217. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  218. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  219. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  220. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  221. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  222. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  223. Zhao J, et al. 2013. Elife. 21:537. PubMed
  224. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  225. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  226. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  227. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  228. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  229. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  230. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  231. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  232. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  233. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  234. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  235. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  236. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  237. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  238. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  239. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  240. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  241. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  242. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  243. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  244. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  245. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  246. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  247. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  248. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  249. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  250. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  251. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  252. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  253. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  254. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  255. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  256. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  257. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  258. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  259. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  260. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  261. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  262. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  263. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  264. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  265. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  266. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  267. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  268. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  269. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  270. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  271. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  272. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  273. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  274. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  275. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  276. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  277. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  278. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  279. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  280. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  281. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  282. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  283. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  284. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  285. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  286. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  287. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  288. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  289. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  290. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  291. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  292. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  293. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  294. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  295. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  296. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  297. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  298. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  299. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  300. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  301. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  302. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  303. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  304. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  305. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  306. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  307. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  308. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  309. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  310. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  311. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  312. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  313. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  314. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  315. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  316. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  317. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  318. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  319. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  320. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  321. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  322. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  323. Trial J, et al. 2020. Geroscience. . PubMed
  324. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  325. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  326. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  327. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  328. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  329. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  330. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  331. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  332. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  333. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  334. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  335. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  336. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  337. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  338. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  339. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  340. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  341. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  342. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  343. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  344. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  345. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  346. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  347. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  348. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  349. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  350. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  351. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  352. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  353. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  354. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  355. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  356. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  357. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  358. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  359. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  360. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  361. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  362. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  363. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  364. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  365. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  366. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  367. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  368. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  369. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  370. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  371. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  372. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  373. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  374. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  375. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  376. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  377. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  378. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  379. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  380. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  381. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  382. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  383. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  384. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  385. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  386. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed

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NA

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