StarBright UltraViolet 740 anti-mouse CD45 antibody, CD45, 30-F11

Pricing & Availability

Clone: 30-F11 (See other available formats)
Regulatory Status: RUO
Other Names: T200, Ly-5, LCA
Isotype: Rat IgG2b, κ
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Product Citations: publications

30-F11_SBUV740_CD45_FC_071025 — C57BL/6 mouse splenocytes were stained with anti-mouse CD45 (clone 30-F11) StarBright UltraViolet 740 (filled histogram). Open histogram represents unstained cells.

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103194

150 μg

316 CHF

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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration: 0.6 mg/mL (the concentration relates to the dye component of the conjugate)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.

StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation³, complement-dependent cytotoxicity^1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting⁷, and spatial biology (IBEX)^10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type: B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What is a “StarBright panel”?: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Will my StarBright panel be provided as a cocktail?: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Are StarBright GMP/ASR products available?: StarBright ASR and GMP products are coming soon.
Are StarBright custom products available?: Yes, please contact our Custom Solutions Team.
Will StarBright isotype controls be offered?: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
What amount of a StarBright conjugate should I use?: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All CD45 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD45	30-F11	FC
PE/Cyanine5 anti-mouse CD45	30-F11	FC
Purified anti-mouse CD45	30-F11	FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45	30-F11	FC
PE/Dazzle™ 594 anti-mouse CD45	30-F11	FC
Brilliant Violet 711™ anti-mouse CD45	30-F11	FC
Brilliant Violet 785™ anti-mouse CD45	30-F11	FC
Brilliant Violet 650™ anti-mouse CD45	30-F11	FC
APC/Fire™ 750 anti-mouse CD45	30-F11	FC
Brilliant Violet 750™ anti-mouse CD45	30-F11	FC
TotalSeq™-A0096 anti-mouse CD45	30-F11	PG
TotalSeq™-B0096 anti-mouse CD45	30-F11	PG
Ultra-LEAF™ Purified anti-mouse CD45	30-F11	FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45	30-F11	FC
Spark NIR™ 685 anti-mouse CD45	30-F11	FC
TotalSeq™-C0096 anti-mouse CD45	30-F11	PG
Spark YG™ 570 anti-mouse CD45	30-F11	IHC-F
PE/Fire™ 640 anti-mouse CD45	30-F11	FC
APC/Fire™ 810 anti-mouse CD45	30-F11	FC
PE/Fire™ 700 anti-mouse CD45	30-F11	FC
APC anti-mouse CD45	30-F11	FC
Biotin anti-mouse CD45	30-F11	FC
FITC anti-mouse CD45	30-F11	FC
APC/Cyanine7 anti-mouse CD45	30-F11	FC
Alexa Fluor® 488 anti-mouse CD45	30-F11	FC,SB
Alexa Fluor® 647 anti-mouse CD45	30-F11	FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45	30-F11	FC
Alexa Fluor® 700 anti-mouse CD45	30-F11	FC,SB
PerCP/Cyanine5.5 anti-mouse CD45	30-F11	FC
PerCP anti-mouse CD45	30-F11	FC
Alexa Fluor® 594 anti-mouse CD45	30-F11	IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45	30-F11	FC,SB
Brilliant Violet 570™ anti-mouse CD45	30-F11	FC
Brilliant Violet 510™ anti-mouse CD45	30-F11	FC
Brilliant Violet 605™ anti-mouse CD45	30-F11	FC
Purified anti-mouse CD45 (Maxpar® Ready)	30-F11	FC,CyTOF®
Spark Violet™ 538 anti-mouse CD45	30-F11	FC
Spark YG™ 593 anti-mouse CD45	30-F11	FC
Spark Blue™ 574 anti-mouse CD45 Antibody	30-F11	FC
Spark Blue™ 515 anti-mouse CD45	30-F11	FC
Spark UV™ 387 anti-mouse CD45	30-F11	FC
PE/Fire™ 810 anti-mouse CD45	30-F11	FC
Spark PLUS UV395™ anti-mouse CD45	30-F11	FC
Spark Red™ 718 anti-mouse CD45	30-F11	FC
Spark YG™ 581 anti-mouse CD45 (Flexi-Fluor™)	30-F11	FC
Spark PLUS V475™ anti-mouse CD45 Antibody	30-F11	FC
StarBright UltraViolet 740 anti-mouse CD45	30-F11	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07.23.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.