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Ultra-LEAF™ Purified anti-human AXL Antibody

Pricing & Availability
Clone
S21005F (See other available formats)
Regulatory Status
RUO
Other Names
AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, AXL Transforming Sequence/Gene, Ty
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S21005F_ULEAF_AXL_Antibody_082825
Recombinant human AXL-Fc chimera (Cat. No. 790902) (black circles) binds to immobilized recombinant human GAS6 (Cat. No. 796806). Ultra-LEAF™ Purified Anti-human AXL (clone S21005F, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF Purified Mouse IgG2a, κ Isotype Control Antibody (Cat. No. 401507) (gray triangles) has no inhibitory effect. This antibody blocks the binding of 15 ng/mL recombinant human AXL-Fc Chimera to 2.0 µg/mL of immobilized recombinant human GAS6. The ND50 range to observe this effect is: 6.0 – 56.0 ng/mL.
  • S21005F_ULEAF_AXL_Antibody_082825
    Recombinant human AXL-Fc chimera (Cat. No. 790902) (black circles) binds to immobilized recombinant human GAS6 (Cat. No. 796806). Ultra-LEAF™ Purified Anti-human AXL (clone S21005F, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF Purified Mouse IgG2a, κ Isotype Control Antibody (Cat. No. 401507) (gray triangles) has no inhibitory effect. This antibody blocks the binding of 15 ng/mL recombinant human AXL-Fc Chimera to 2.0 µg/mL of immobilized recombinant human GAS6. The ND50 range to observe this effect is: 6.0 – 56.0 ng/mL.
  • S21005F_ULEAF_AXL_Antibody_2_082825
    Ultra-LEAF™ Purified Anti-human AXL (clone S21005F) (purple squares) blocks the binding of immobilized recombinant human GAS6 (Cat. No. 796806) to recombinant human AXL-Fc Chimera (black circles) at ND50=33.0 ng/mL, whereas the main competitor antibody blocks at ND50=104 ng/mL (orange squares). Ultra-LEAF™ Purified Mouse IgG2a, k Isotype Control Antibody (Cat. No. 401507) (gray triangles) showed no inhibitory effect.
Cat # Size Price Quantity Check Availability Save
648856 100 µg 110 CHF
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648857 1 mg 149 CHF
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Description

AXL is a receptor tyrosine kinase member of the TAM family that includes Tyro3, AXL, and Mer. The members of this family are type I receptors characterized by two immunoglobulin (Ig)-like and fibronectin III (FNIII) domains in the extracellular domain, a transmembrane domain, and a kinase intracellular domain. The TAM receptors have common ligands, such as GAS-6 and protein S. GAS-6 binds to the three receptors, meanwhile protein S binds only to Tyro3 and Mer. GAS-6 has the highest affinity for AXL, compared to Tyro3 and Mer. AXL is activated by different mechanisms: Gas-6-dependent dimerization, ligand-independent dimerization, and heteromeric dimerization with different receptors that are not members of the TAM family, such as IFNAR, EGFR, PDGFR, and FLT3. Soluble forms of AXL are present in human and murine plasma. They derived of the proteolytic cleavage of the extracellular domain. Soluble forms also bind to GAS-6, acting as inhibitors of AXL-mediated cell activation. AXL is overexpressed in many cancers, such as lung, breast, colon, gastric, ovarian, prostate, thyroid, myeloid leukemia, and chronic lymphocytic leukemia to mention some, and its expression has been associated with poor prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human AXL extracellular domain (R&D Systems 10448-AL)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 15 ng/mL of Human AXL-Fc Chimera (Cat. No. 790902) to its ligand Human GAS6 (Cat. No. 796806), immobilized at 2 μg/mL. ND50 range: 6.0 – 56.0 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Ubiquitously expressed at high levels in platelets, monocytes/macrophages, endothelial cells, mesenchymal, epithelial, and hematopoietic cell lines.

Function
Cellular growth, survival, and differentiation. Promotes tumor cell metastasis, therapeutic resistance, and fosters disease progression.
Interaction
Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Mesenchymal cells, Monocytes, NK cells, Platelets
Ligand/Receptor
GAS-6
Cell Type
Dendritic cells, NK cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cardiovascular Biology, Cell Death, Cell Proliferation and Viability, Immuno-Oncology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, Cytokines/Chemokines
Antigen References
  1. O'Bryan JP, et al. 1991. Mol. Cell. Biol. 11:5016.
  2. Angelillo-Scherrer A, et al. 2001. Nat. Med. 7:215.
  3. Budagian V, et al. 2005. Mol. Cell. Biol. 25:9324.
  4. Schmidt T, et al. 2012. Cell. Mol. Life Sci. 69:1391.
  5. Wu X, et al. 2014. Oncotarget. 5:9546.
  6. Axelrod H, Pienta KJ, 2014. Oncotarget. 5:8818.
Gene ID
NA
UniProt
View information about AXL on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08.28.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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