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Ultra-LEAF™ Purified anti-human CD55 Antibody

Pricing & Availability
Clone
W24215H (See other available formats)
Regulatory Status
RUO
Other Names
DAF (Decay Accelerating Factor)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a. W24215H_ULEAF_CD55_Antibody_1_100225
Immobilized Recombinant human CD55 binds to recombinant human CD97-Fc Chimera (black circles). Ultra-LEAF™ Purified Anti-human CD55 (clone W24215H, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400644, gray triangles) has no inhibitory effect. This antibody blocks the binding of 200 ng/mL recombinant human CD97-Fc Chimera to 2.0 µg/mL of immobilized recombinant human CD55. The ND50 range to observe this effect is: 0.2– 1.2 µg/mL.
  • a. W24215H_ULEAF_CD55_Antibody_1_100225
    Immobilized Recombinant human CD55 binds to recombinant human CD97-Fc Chimera (black circles). Ultra-LEAF™ Purified Anti-human CD55 (clone W24215H, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400644, gray triangles) has no inhibitory effect. This antibody blocks the binding of 200 ng/mL recombinant human CD97-Fc Chimera to 2.0 µg/mL of immobilized recombinant human CD55. The ND50 range to observe this effect is: 0.2– 1.2 µg/mL.
  • b. W24215H_ULEAF_CD55_Antibody_2_100225
    Ultra-LEAF™ Purified Anti-human CD55 (clone W24215H, purple squares) blocks the binding of immobilized recombinant human CD55 to recombinant human CD97-Fc Chimera (black circles) at ND50= 0.4 µg/mL, whereas the main competitor antibody blocks at ND50= 0.65 µg/mL (orange squares). Ultra-LEAF™ Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400644, gray triangles) showed no inhibitory effect.
Cat # Size Price Quantity Check Availability Save
648888 100 µg 225 CHF
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648889 1 mg 720 CHF
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Description

CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). CD55 is expressed on hematopoietic cells, including erythrocytes, and many non-hematopoietic cells. CD55 accelerates the dissociation of the components of the C3-convertases (namely C2a from C4b in the C4bC2a complex, a C3-convertase of the classical pathway, and factor Bb from the C3bBb complex, a C3-convertase of the alternative pathway) to protect cells from inappropriate damage caused by autologous complement. CD55 has been reported to reduce the efficiency of NK cell lysis and induce signal transduction in T cells. CD55 has also been shown to interact with CD97 and bind to Coxsackie and Echovirus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD55 recombinant protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 2 μg/mL immobilized human CD55 (Cat. No. TBD) to its receptor human CD97 (Cat. No. TBD) at 200 ng /mL. ND50 range: 0.2 – 1.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
GPI-anchored regulator of complement activation (RCA), type I membrane glycoprotein
Distribution

Hematopoietic cells including erythrocytes and many non-hematopoietic cells

Function
Protects against inappropriate complement activation and cell damage, induces signal transduction, reduces the efficiency of NK cell lysis
Ligand/Receptor
C3b, C4b, CD97, Coxsackie viruses
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Hamann J, et al. 1996. J. Exp. Med. 184:1185.
3. Fujita T, et al. 1987. J. Exp. Med. 166:1221.

Gene ID
NA
UniProt
View information about CD55 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 10.09.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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