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Alexa Fluor® 555 anti-α Smooth Muscle Actin Antibody

Pricing & Availability
Clone
1A4 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, aortic smooth muscle (αSMA), alpha 2 actin (ACTA2), alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
Isotype
Mouse IgG2a, κ
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Product Citations
publications
a. 1A4_A555_Asmooth_IHC-P_100925
IHC staining with Alexa Fluor® 555 anti-α-Smooth Muscle Actin (clone 1A4) on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with Alexa Fluor® 555 anti-mouse IgG2a Isotype Control (panel A) or Alexa Fluor® 555 anti-α-Smooth Muscle Actin antibody (clone 1A4) (panel B). Images were captured with a 40X objective. Scale: 50 µm
  • a. 1A4_A555_Asmooth_IHC-P_100925
    IHC staining with Alexa Fluor® 555 anti-α-Smooth Muscle Actin (clone 1A4) on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with Alexa Fluor® 555 anti-mouse IgG2a Isotype Control (panel A) or Alexa Fluor® 555 anti-α-Smooth Muscle Actin antibody (clone 1A4) (panel B). Images were captured with a 40X objective. Scale: 50 µm
  • b. 1A4_A555_Asmooth_ICC_100925
    NIH/3T3 cells (low-expressing cell line, panel A) and aortic vascular smooth muscle cells (high-expressing cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 555 anti-α-Smooth Muscle Actin (clone 1A4), and nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
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614859 25 µg 148€
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Description

Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins expressed in mammals. α-smooth muscle actin is widely expressed in contractile cells, most notably the mural cells that line arteries and arterioles. Overexpression of α-smooth muscle actin is a hallmark of fibrosis, a thickening or scarring of damaged tissue.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal synthetic peptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-100% ice-cold methanol only
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol

Antigen Details

Structure
α smooth muscle actin is a 377 amino acid protein with a predicted molecular weight of 42 kD.
Distribution

Cytoplasmic, cytoskeleton

Function
Cell motility
Interaction
α smooth muscle actins bind to myosins to produce motive force
Cell Type
Mesenchymal Stem Cells, Monocytes
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References
  1. Brennan PA, et al. 2000. J Oral Pathol Med. 29:279-83.
  2. Rizeq MN, et al. 1994. Hum Pathol. 25:671-7.
  3. Schmitt-Graff A, et al. 1991. Am J Pathol. 138::1233-42
  4. Roholl PJM, et al. 1990. Hum Pathol. 21:1269-74.
  5. Alberts B, et al. 1989. Eds Molecular biology of the cell. 2:613.
  6. Skalli O, et al. 1986. J Cell Biol. 103:2787-96.
Gene ID
59 View all products for this Gene ID
UniProt
View information about alpha Smooth Muscle Actin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/09/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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