Loading...

Brilliant Violet 605™ anti-human TLR7 Antibody

Pricing & Availability
Clone
S18024F (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like receptor (TLR) 7, CD287
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
S18024F_BV605_TLR7_Antibody_1_043025
Human peripheral blood mononuclear cells were surface stained with anti-human CD14 (clone HCD14) Brilliant Violet 421™ and anti-human CD304 (clone 12C2) Brilliant Violet 711™. Cells were then fixed with Fixation Buffer (Cat. No. 420801), permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) and intracellularly stained with anti-human TLR7 (clone S18024F) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram). Data shown were gated on the CD14-negative CD304-positive plasmacytoid dendritic cell population.
  • S18024F_BV605_TLR7_Antibody_1_043025
    Human peripheral blood mononuclear cells were surface stained with anti-human CD14 (clone HCD14) Brilliant Violet 421™ and anti-human CD304 (clone 12C2) Brilliant Violet 711™. Cells were then fixed with Fixation Buffer (Cat. No. 420801), permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) and intracellularly stained with anti-human TLR7 (clone S18024F) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram). Data shown were gated on the CD14-negative CD304-positive plasmacytoid dendritic cell population.
Compare all formats See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Check Availability Save
376915 25 tests 177€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Toll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α/β and other inflammatory cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone S18024F can be used for blocking R848-activated IL8 cytokine secretion (tested on 293XL/TLR7 cell line) and for intracellular flow cytometry.

Antigen Details

Structure
Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family
Distribution

Expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes, Monocytes

Function
Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production
Interaction
B cells, Dendritic cells
Ligand/Receptor
Single-stranded RNA
Cell Type
B cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling
Gene ID
51284 View all products for this Gene ID
UniProt
View information about TLR7 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/28/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account