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Purified anti-c-Fos Antibody

Pricing & Availability
Clone
W23257D (See other available formats)
Regulatory Status
RUO
Other Names
Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog, G0/G1 Switch Regulatory Protein 7, Activator Protein 1, GOS7, cfos
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
A. W23257D_PURE_c-Fos_WB_61925
Whole cell extracts (15 µg protein) from serum-starved cells treated without (-) or with (+) PMA (200 nM) for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with Purified anti-c-Fos (clone W23257D) overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: molecular weight marker
  • A. W23257D_PURE_c-Fos_WB_61925
    Whole cell extracts (15 µg protein) from serum-starved cells treated without (-) or with (+) PMA (200 nM) for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with Purified anti-c-Fos (clone W23257D) overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: molecular weight marker
  • B. W23257D_PURE_c-Fos_ICC_61925
    Serum-starved HeLa cells treated without (panels A and C) or with PMA for 4 hours (200 nM, panels B and D) were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-c-Fos (clone W23257D), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Cells were also stained with Alexa Fluor® 488 anti-Tubulin-α (Cat. No. 627906) (green) and nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The images were captured and merged on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • C. W23257D_PURE_c-Fos_ICFC_61925
    Serum-starved HeLa treated without (open histogram, dim control) or with PMA (200 nM for 4 hr)(filled histogram, positive control) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-c-Fos (clone W23257D) or Purified Rat IgG2b, κ Isotype Ctrl (open histogram, dashed line) (Cat. No. 400602) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416)
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657051 25 µg 132€
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657052 100 µg 368€
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Description

The c-Fos protein, or FBJ murine viral (v-Fos) oncogene homolog, is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the AP-1 (Activator Protein-1) complex, which regulates critical cellular processes, including cell proliferation, differentiation, and survival. C-Fos has been demonstrated to play a critical role in angiogenesis, bone development, and in the progression of cancer. C-Fos is also often used as an indirect marker of neuronal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human c-Fos
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
- Fixation Buffer (Cat. No. 420801) with 0.5 % Triton X-100
- Fixation Buffer (Cat. No. 420801) with 100% ice-cold methanol

For use in intracellular flow cytometry (ICFC), it is recommended to fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401).

Antigen Details

Structure
c-Fos is a 380 amino acid protein with a predicted molecular weight of 41 kD.
Distribution

Nucleus

Function
FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation.
Interaction
Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors
Antigen References
  1. Dony C and Gruss P. 1987. Nature. 328:711-4.
  2. Grigoriadis AE, et al. 1994. Science. 266:443-8.
  3. Glover JN and Harrison SC. 1995. Nature. 373:257-61.
Gene ID
2353 View all products for this Gene ID
UniProt
View information about c-Fos on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/19/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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