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Purified anti-human BMP-4 Antibody

Pricing & Availability
Clone
A22044E (See other available formats)
Regulatory Status
RUO
Other Names
BMP-4, Bone morphogenetic protein 4, Bone morphogenetic protein 2B, BMP2B, BMP2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A22044E_PURE_BMP-4_092425
Direct ELISA showing pure anti-human BMP-4 (clone A22044E) bound to plate-immobilized recombinant human BMP-4. The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP anti-mouse IgG (Cat No. 405306). TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A22044E_PURE_BMP-4_092425
    Direct ELISA showing pure anti-human BMP-4 (clone A22044E) bound to plate-immobilized recombinant human BMP-4. The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP anti-mouse IgG (Cat No. 405306). TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450 nm.
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612159 100 µg 276€
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Description

Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGFbeta) superfamily. BMPs play a key role in embryonic development, especially during heart, neural and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptor and type II / IIB activin receptors. The BMP-4 gene is conserved across human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, and zebrafish species. BMP-2 and BMP-4 are highly similar, with a 92% amino acid sequence identity. BMP-4 is required for mesoderm formation and patterning in mice, and BMP-4 KO mice are embryonic-lethal. BMP-4 has an important role in postnatal tooth cytodifferentiation. Overexpression of BMP-4 has been associated with fibrodysplasia ossificans progressiva (FOP). Overexpression of BMP-4 has also been associated with cancer, including melanoma, hepatocellular carcinoma, adenocarcinoma and colorectal cancer cell lines. This overexpression can promote migratory and invasive properties of cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant BMP-4 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A22044E) could function as a detection antibody when paired with purified anti-human BMP-4 antibody (Cat No. TBD) (clone 20137E) as the capture antibody and using Recombinant Human BMP-4 (carrier-free) (Cat Nos. 795604, 795606, 591201, 595202) protein as the assay standard.

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Osteoblast, odontoblast, ameloblasts, megakaryocytes, endothelial cells. Lymphoblastoid cell lines from patients with fibrodysplasia ossificans progressiva.

Function
Neural Stem Cells, mesenchymal stem cells, hematopoietic stem and progenitors, embryonic stem cells
Interaction
Condroblast, osteoblast, retinoblastoma cells
Ligand/Receptor
Type I receptors BMPR-IA (ALK-3) and BMPR-IB (ALK-6)
Cell Type
Astrocytes, Embryonic Stem Cells, Mesenchymal Stem Cells, Oligodendrocytes
Biology Area
Cardiovascular Biology, Cell Biology, Immuno-Oncology, Neurodegeneration, Stem Cells
Molecular Family
Growth Factors
Antigen References
  1. Zhong Z, et al. 2022. Bio Protoc. 12: . PubMed
  2. Mao QQ, et al. 2024. Exp Ther Med. 74:27. PubMed
  3. Chen WJ, et al. 2024. Cell Death Discov. 468:10. PubMed
Gene ID
652 View all products for this Gene ID
UniProt
View information about BMP-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/24/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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