Loading...

RBC Lysis Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Red blood cell lysis buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420301 100 mL 52€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
420302 500 mL 165€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  2. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  3. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  4. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  5. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  6. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  7. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  8. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  9. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  10. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  12. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  13. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  14. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  15. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  16. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  17. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  18. Melendez E, et al. 2022. Development. 149: . PubMed
  19. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  20. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  21. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  22. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  23. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  24. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  25. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  26. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  27. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  28. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  29. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  30. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  31. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  32. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  33. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  34. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  35. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  36. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  37. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  38. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  39. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  40. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  41. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  42. Feng J, et al. 2022. Immunity. 55:405. PubMed
  43. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  44. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  45. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  46. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  47. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  48. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  49. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  50. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  51. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  52. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  53. Le DT, et al. 2023. iScience. 26:106059. PubMed
  54. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  55. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  56. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  57. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  58. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  59. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  60. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  61. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  62. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  63. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  64. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  65. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  66. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  67. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  68. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  69. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  70. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  71. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  72. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  73. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  74. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  75. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  76. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  77. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  78. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  79. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  80. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  81. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  82. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  83. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  84. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  85. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  86. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  87. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  88. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  89. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  90. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  91. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  92. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  93. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  94. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  95. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  96. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  97. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  98. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  99. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  100. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  101. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  102. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  103. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  104. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  105. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  106. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  107. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  108. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  109. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  110. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  111. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  112. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  113. Meng S, et al. 2022. Exp Anim. . PubMed
  114. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  115. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  116. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  117. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  118. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  119. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  120. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  121. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  122. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  123. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  124. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  125. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  126. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  127. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  128. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  129. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  130. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  131. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  132. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  133. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  134. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  135. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  136. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  137. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  138. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  139. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  140. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  141. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  142. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  143. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  144. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  145. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  146. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  147. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  148. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  149. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  150. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  151. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  152. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  153. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  154. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  155. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  156. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  157. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  158. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  159. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  160. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  161. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  162. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  163. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  164. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  165. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  166. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  167. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  168. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  169. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  170. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  171. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  172. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  173. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  174. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  175. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  176. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  177. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  178. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  179. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  180. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  181. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  182. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  183. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  184. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  185. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  186. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  187. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  188. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  189. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  190. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  191. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  192. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  193. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  194. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  195. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  196. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  197. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  198. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  199. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  200. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  201. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  202. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  203. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  204. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  205. Sci T 2013. Toxicol Sci. 136:72. PubMed
  206. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  207. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  208. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  209. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  210. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  211. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  212. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  213. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  214. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  215. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  216. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  217. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  218. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  219. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  220. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  221. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  222. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  223. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  224. Zhao J, et al. 2013. Elife. 21:537. PubMed
  225. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  226. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  227. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  228. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  229. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  230. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  231. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  232. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  233. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  234. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  235. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  236. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  237. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  238. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  239. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  240. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  241. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  242. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  243. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  244. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  245. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  246. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  247. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  248. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  249. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  250. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  251. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  252. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  253. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  254. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  255. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  256. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  257. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  258. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  259. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  260. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  261. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  262. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  263. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  264. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  265. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  266. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  267. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  268. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  269. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  270. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  271. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  272. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  273. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  274. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  275. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  276. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  277. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  278. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  279. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  280. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  281. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  282. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  283. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  284. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  285. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  286. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  287. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  288. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  289. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  290. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  291. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  292. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  293. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  294. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  295. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  296. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  297. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  298. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  299. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  300. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  301. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  302. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  303. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  304. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  305. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  306. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  307. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  308. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  309. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  310. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  311. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  312. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  313. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  314. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  315. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  316. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  317. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  318. Trial J, et al. 2020. Geroscience. . PubMed
  319. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  320. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  321. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  322. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  323. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  324. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  325. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  326. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  327. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  328. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  329. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  330. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  331. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  332. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  333. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  334. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  335. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  336. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  337. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  338. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  339. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  340. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  341. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  342. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  343. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  344. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  345. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  346. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  347. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  348. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  349. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  350. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  351. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  352. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  353. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  354. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  355. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  356. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  357. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  358. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  359. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  360. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  361. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  362. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  363. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  364. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  365. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  366. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  367. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  368. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  369. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  370. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  371. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  372. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  373. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  374. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  375. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  376. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  377. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  378. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  379. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  380. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  381. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  382. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  383. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  384. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  385. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  386. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account