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Swift-Click™ Green EdU Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
EdU, 5-ethynyl-2′-deoxyuridine, EdU click-chemistry
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Product Citations
publications
A_Swift_Click_Green_EdU_Kit_FC_1_040124
Jurkat cells pulsed with 5-ethynyl-2′-deoxyuridine (EdU) for 3 hours (right) or untreated control cells (left) were stained using the Swift-Click™ Green EdU Kit and DAPI.
  • A_Swift_Click_Green_EdU_Kit_FC_1_040124
    Jurkat cells pulsed with 5-ethynyl-2′-deoxyuridine (EdU) for 3 hours (right) or untreated control cells (left) were stained using the Swift-Click™ Green EdU Kit and DAPI.
  • B_Swift_Click_Green_EdU_Kit_FC_2_040124
    Representative cell cycle analysis data of bone marrow cells harvested from a C57BL/6 mouse injected intraperitoneally with 1 mg EdU. The bone marrow was harvested 2 hours post-injection and stained with the Swift-Click™ Green EdU Kit and DAPI.
  • C_Swift_Click_Green_EdU_Kit_ICC_040124
    Representative ICC staining using the Swift-Click Green EdU Kit. A549 cells were grown on coverslips in the presence of EdU (10 uM) for 2 hours and fixed with Fixation Buffer (Cat. No. 420801) for 30 minutes. Fixed cells were blocked with 3% BSA for 10 minutes, permeabilized using 0.5% Triton X-100 for 20 minutes, and then stained with Azide-Green cocktail (green) for 30 minutes at ambient temperature in the dark. Cells were then stained with 2.5 ug/mL of anti-PCNA Alexa Fluor® 594 (red) for 1 hour at ambient temperature in the dark. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 40X objective.
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423702 100 tests 624€
 
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Description

The Swift-Click™ Green EdU Kit allows the rapid fluorescent detection of incorporated nucleoside analog in proliferating cells for cell cycle analysis. The staining procedure consists of labeling cells through incubation with nucleoside analog 5-ethynyl-2´-deoxyuridine (EdU), followed by cell fixation and permeabilization using our True-Nuclear™ Transcription Factor Buffer Set, and fluorescent EdU labeling. Fluorescent EdU labeling is catalyzed by Click chemistry, a Nobel Prize-recognized chemical reaction that rapidly links fluorophore-azide molecules to the alkyne group present in EdU in the presence of Cu2+ ions. This method bypasses radioactivity use, as well as long and harsh permeabilization and chromatin denaturation steps, allowing for a faster, simpler-to-follow procedure for cell cycle analysis.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents

The Swift-Click™ Green EdU Kit contains two components:
 

Component A (store at -20°C)

  • EdU (1 vial)
  • Azide-Green (2 vials)
  • Buffer additive (2 vials)
  • Dimethyl Sulfoxide (DMSO) (1 vial)

Component B (store at 4°C)

  • True-Nuclear™ 4X Fix Concentrate (1 bottle)
  • True-Nuclear™ Fix Diluent (1 bottle)
  • True-Nuclear™ 10X Perm Buffer (1 bottle)
  • Copper Sulfate (CuSO4) (1 bottle)
  • DAPI (1 vial)
  • 7-AAD (1 vial)


To obtain lot-specific expiration dates of the two kit components, please search the component lot numbers individually using our Certificate of Analysis online tool.

Product Details

Storage & Handling
Varies by component, see manual for instructions.
Application

ICFC - Quality tested
ICC - Verified
Recommended Usage

See manual for instructions.

Application Notes

Excitation Laser: 488 nm, Excitation Max: 495 nm, Emission Max: 519 nm

Application References

(PubMed link indicates BioLegend citation)
  1. Mitchison TJ, et al. 2008. Proc Natl Acad Sci U S A. 105:2415-2420.

Antigen Details

Structure
Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Function
EdU can be incorporated into DNA and substitute thymidine residues and used to identify cells undergoing DNA replication.
Bioactivity
EdU will be incorporated into DNA during the cell cycle's synthesis phase.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, DNA Repair/Replication, Immunology
Molecular Family
Nuclear Markers
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/26/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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