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Alexa Fluor® 488 anti-human EOS Antibody

Pricing & Availability
Clone
W16032B (See other available formats)
Regulatory Status
RUO
Other Names
Ikaros family zinc finger protein 4 (IKZF4), ZMFN1A4, KIAA1782
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16032B_AF488_EOS_Antibody_032625
Human peripheral blood leukocytes were surface stained with anti-human CD4 (clone RPA-T4) APC and then fixed, permeabilized, and intracellularly stained with the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Cells were then stained with anti-human FOXP3 (clone 206D) Brilliant Violet 421™ and anti-human EOS (clone W16032B) Alexa Fluor® 488 (left) or rat IgG2a, κ Alexa Fluor® 488 isotype control (right). Data shown was gated on the CD4+ lymphocyte population.
  • W16032B_AF488_EOS_Antibody_032625
    Human peripheral blood leukocytes were surface stained with anti-human CD4 (clone RPA-T4) APC and then fixed, permeabilized, and intracellularly stained with the True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Cells were then stained with anti-human FOXP3 (clone 206D) Brilliant Violet 421™ and anti-human EOS (clone W16032B) Alexa Fluor® 488 (left) or rat IgG2a, κ Alexa Fluor® 488 isotype control (right). Data shown was gated on the CD4+ lymphocyte population.
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397405 25 tests 132€
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397406 100 tests 317€
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Description

EOS also known as IKZF4 is an IKAROS Family Member expressed in lymphocytes, specifically in Tregs. It plays an important role in the inhibitory function of Tregs. It may be involved in the development of the central and peripheral nervous systems as well as lymphoid development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human EOS recombinant protein (271-520 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clones W16032A and W16032B cross-block each other and likely recognize different epitopes.

Antigen Details

Structure
IKAROS family member
Distribution

Lymphocytes, specifically in Tregs

Function
Essential for inhibitory function of Tregs
Interaction
Binds to 5'GGGAATRCC-3' Ikaros-binding sequence
Antigen References
  1. Honma Y, et al. 1999. FEBS Lett. 447:76-80. 
  2. Perdomo J, et al. 2000. J. Biol. Chem. 275:38347-54. 
  3. Westman BJ, et al. 2004. Biochemistry. 43:13318-27. 
  4. Pan F, et al. 2009. Science. 10.1126/science.1176077. 
Gene ID
64375 View all products for this Gene ID
UniProt
View information about EOS on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/04/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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