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LEAF™ Purified anti-rat IL-10 Antibody

Pricing & Availability
Clone
Poly23A23 (See other available formats)
Regulatory Status
RUO
Other Names
CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL-10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly23A23_LEAF_IL-10_Block_021825
Recombinant rat IL-10 (black circles) stimulates the production of mouse IL-6 in Raw264.7 cells. LEAF™ purified anti-rat IL-10 (clone Poly23A23) (purple squares) inhibits the effect of recombinant rat IL-10 at 20 ng/mL on Raw264.7 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control antibody (gray triangles) does not have the effect. ND50 range: 0.1 – 1.0 µg/mL
  • Poly23A23_LEAF_IL-10_Block_021825
    Recombinant rat IL-10 (black circles) stimulates the production of mouse IL-6 in Raw264.7 cells. LEAF™ purified anti-rat IL-10 (clone Poly23A23) (purple squares) inhibits the effect of recombinant rat IL-10 at 20 ng/mL on Raw264.7 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control antibody (gray triangles) does not have the effect. ND50 range: 0.1 – 1.0 µg/mL
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513904 100 µg 220€
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Description

IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. It inhibits interferon IFNγ synthesis in Th1 cell, and therefore it was initially called cytokine synthesis inhibiting factor (CSIF). Macrophages are the main source of IL-10 and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-κB dependent), tumor necrosis factor TNFα (via TNF receptor p55, NF-kB-dependent), catecholamines, and IL-1. IL-10 controls inflammatory processes by suppressing the expression of proinflammatory cytokines, chemokines, adhesion molecules, as well as antigen-presenting and costimulatory molecules in monocytes/macrophages, neutrophils, and T cells. IL-10 inhibits the production of proinflammatory mediators by monocytes and macrophages such as endotoxin- and IFNγ-induced release of IL-1α, IL-6, IL-8, G-CSF, GM-CSF, and TNFα. In addition, it enhances the production of anti-inflammatory mediators such as IL-1RA and soluble TNFα receptors. IL-10 inhibits the capacity of monocytes and macrophages to present antigen to T cells. This is realized by down-regulation of constitutive and IFNγ-induced cell surface levels of MHC class II, of costimulatory molecules such as CD86 and of some adhesion molecules such as CD58.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant Rat IL-10
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/μg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the neutralization of mouse IL-6 production induced by recombinant rat IL-10 at 20 ng/mL on Raw264.7 cells. ND50 range: 0.1 - 1.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Monomer
Distribution

IL-10 is produce by Th2 cells, macrophages, DCs, B cells, CD8+ T cells, regulatory T cells (Tregs), Th1 cells and Th17 cells. In addition, IL-10 is expressed by monocytes, B cells, eosinophils, and mast cells.

Function
IL-10 is an immunoregulatory cytokine. Its main function is the limitation and termination of inflammatory responses and the regulation of differentiation and proliferation of several immune cells such as T cells, B cells, natural killer cells, antigen-presenting cells, mast cells, and granulocytes.
Interaction
IL-10R is expressed in monocytes, NK, B, and T cells. In addition, Langerhans cells, dermal dendritic cells, eosinophils, mast cells, and endothelial cells can respond to IL-10.
Ligand/Receptor
IL-10 binds to their receptors IL-10R1 and IL-10R2, and initiates a STAT3-dependent signaling cascade.
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Feng L, et al. 1993. Bichem Biophys Res Commun. 192:452-8.
  2. Fiorentino DF, et al. 1989, J Exp Med. 170:2081-95.
  3. Ho AS, et al. 1993. P Natl Acad Sci USA. 90:11267.
  4. Hart PH, et al. 1996. J Immunol. 157:3672-80.
  5. Asadullah K, et al. 2003. Pharmacol Rev. 55:241-69.
  6. Mosser DM and Zhang X. 2008. Immunol Rev. 226:205.
  7. Maynard CL and Weaver CT. 2008. Immunol. Rev. 226:219.
Gene ID
25325 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 0    Revision Date: 01/01/0001

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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