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PE anti-human CD158b (KIR2DL2/KIR2DL3) Antibody

Pricing & Availability
Clone
S22037G (See other available formats)
Regulatory Status
RUO
Other Names
CD158b1 (KIR2DL2, p58.2, NKAT6), CD158b2 (KIR2DL3, p58.3, NKAT2)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
S22037G_PE_CD158b_Antibody_1_062525-2
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone QA17A16) APC and anti-human CD158b (KIR2DL2/KIR2DL3) (clone S22037G) PE (left) or mouse IgG2a, κ PE isotype control (right).
  • S22037G_PE_CD158b_Antibody_1_062525-2
    Human peripheral blood lymphocytes were stained with anti-human CD56 (clone QA17A16) APC and anti-human CD158b (KIR2DL2/KIR2DL3) (clone S22037G) PE (left) or mouse IgG2a, κ PE isotype control (right).
See PE spectral data
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317510 100 tests 259€
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Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans.

CD158b1 and CD158b2 are 38 kD two extracellular Ig domain glycoprotein that displays a long cytoplasmic tail with ITIM motifs. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR2DL2 and KIR2DL3 have been reported to bind to certain HLA-C antigens on a target cell (HLA-C*01, HLA-C*03, HLA-C*07, for example).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human KIR2DL2 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone S22037G can block the binding of clone DX27 on target cells.

Based on in-house testing on different KIR-expressing transfectants, clone S22037G does not interact with other KIR protein family members.

Antigen Details

Structure
38 kD two Ig-like extracellular domain glycoprotein
Distribution

Subset of NK cells, subset of T cells

Function
NK cell regulation
Ligand/Receptor
Some HLA-C molecules
Cell Type
Lymphocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Vilches C, et al. 2002. Annu Rev Immunol. 92.107361.
  2. Middleton D, et al. 2010. Immunol. 123:8.
  3. Moesta A.K, et al. 2012. Frontiers Immu. 3.336.
  4. Moradi S, et al. 2021. Nature Commu. 12.2173.
Gene ID
3803 View all products for this Gene ID 3804 View all products for this Gene ID
UniProt
View information about CD158b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/25/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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