Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Denmark (DKK) DKK
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Norway (NOK) NOK
Sweden (SEK) SEK
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Regulatory Status
- RUO
- Other Names
- Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, CTLA1, Serine Protease B, CSP-B, CSPB, Cytotoxic serine protease B, CCP1, Cytotoxic cell protease 1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 439209 | 1 Pre-coated Plate | 472€ | ||||
Human Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and natural killer (NK) cells. Human Granzyme B is one of the five members of the human granzyme family which includes Granzymes A, B, G, H, and K. Granzyme B is expressed as a 32 kD protein encoded by the GZMB gene. While Granzyme B is mostly expressed by CTL (Cytotoxic T Lymphocyte) and NK cells, it is also expressed in resting and activated plasmacytoid dendritic cells. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. Granzyme B and perforin have been shown to induce CTL-mediated target cell DNA fragmentation and apoptosis. Once released from the CTL, Granzyme B binds its receptor, the mannose-6-phosphate/insulin-like growth factor II receptor, and is endocytosed but remains arrested in endocytic vesicles until released by perforin. Once in the cytosol, Granzyme B targets caspase-3 and other caspases directly or indirectly through the mitochondria, initiating the caspase cascade which leads to DNA fragmentation and apoptosis. Elevated Granzyme B levels have been linked with various disease processes.
The BioLegend RAPID MAX™ Human Granzyme B ELISA Kit is a one step, 75-minute enzyme-linked immunosorbent sandwich assay (ELISA) that includes a 96-well strip plate that is pre-coated with an anti-tag antibody. Standards and samples are added into the wells, followed by an antibody cocktail. The antibody cocktail consists of a tag-conjugated capture antibody and an HRP-conjugated detection antibody. This kit is specifically designed for the accurate quantitation of human Granzyme B cell culture supernatant. This kit is analytically validated with ready-to-use reagents
Kit Details
- Kit Contents
-
- RAPID MAX™ Pre-coated A 96-well Strip Microplate A
- Human Granzyme B Capture Antibody Concentrate (200X)
- Human Granzyme B Detection Antibody Concentrate (200X)
- Human Granzyme B Standard
- Assay Diluent A (5X), 12 mL
- Substrate Solution F
- Wash Buffer (20X)
- Stop Solution
- Plate Sealers
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 9.5 ± 7.2 pg/mL
- Standard Range
- 78-5000 pg/mL
Antigen Details
- Cell Type
- Dendritic cells, Granulocytes, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Enzymes and Regulators, Proteases
- Gene ID
- NA
- UniProt
- View information about Granzyme B on UniProt.org
Follow Us