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RBC Lysis Buffer (10X)

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RUO
Other Names
Red blood cell lysis buffer
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420301 100 mL 52€
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420302 500 mL 165€
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Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  2. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  3. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  4. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  5. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  6. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  7. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  8. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  9. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  10. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  11. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  12. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  13. Melendez E, et al. 2022. Development. 149: . PubMed
  14. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  15. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  16. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  17. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  18. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  19. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  20. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  21. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  22. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  23. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  24. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  25. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  26. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  27. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  28. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  29. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  30. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  31. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  32. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  33. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  34. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  35. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  36. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  37. Feng J, et al. 2022. Immunity. 55:405. PubMed
  38. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  39. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  40. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  41. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  42. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  43. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  44. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  45. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  46. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  47. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  48. Le DT, et al. 2023. iScience. 26:106059. PubMed
  49. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  50. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  51. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  52. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  53. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  54. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  55. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  56. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  57. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  58. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  59. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  60. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  61. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  62. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  63. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  64. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  65. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  66. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  67. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  68. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  69. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  70. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  71. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  72. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  73. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  74. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  75. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  76. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  77. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  78. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  79. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  80. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  81. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  82. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  83. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  84. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  85. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  86. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  87. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  88. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  89. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  90. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  91. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  92. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  93. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  94. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  95. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  96. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  97. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  98. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  99. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  100. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  101. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  102. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  103. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  104. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  105. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  106. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  107. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  108. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  109. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  110. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  111. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  112. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  113. Meng S, et al. 2022. Exp Anim. . PubMed
  114. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  115. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  116. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  117. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  118. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  119. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  120. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  121. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  122. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  123. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  124. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  125. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  126. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  127. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  128. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  129. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  130. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  131. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  132. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  133. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  134. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  135. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  136. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  137. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  138. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  139. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  140. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  141. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  142. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  143. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  144. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  145. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  146. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  147. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  148. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  149. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  150. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  151. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  152. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  153. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  154. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  155. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  156. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  157. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  158. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  159. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  160. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  161. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  162. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  163. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  164. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  165. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  166. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  167. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  168. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  169. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  170. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  171. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  172. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  173. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  174. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  175. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  176. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  177. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  178. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  179. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  180. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  181. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  182. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  183. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  184. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  185. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  186. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  187. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  188. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  189. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  190. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  191. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  192. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  193. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  194. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  195. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  196. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  197. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  198. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  199. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  200. Sci T 2013. Toxicol Sci. 136:72. PubMed
  201. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  202. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  203. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  204. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  205. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  206. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  207. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  208. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  209. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  210. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  211. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  212. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  213. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  214. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  215. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  216. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  217. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  218. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  219. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  220. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  221. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  222. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  223. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  224. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  225. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  226. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  227. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  228. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  229. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  230. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  231. Zhao J, et al. 2013. Elife. 21:537. PubMed
  232. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  233. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  234. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  235. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  236. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  237. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  238. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  239. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  240. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  241. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  242. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  243. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  244. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  245. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  246. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  247. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  248. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  249. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  250. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  251. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  252. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  253. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  254. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  255. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  256. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  257. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  258. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  259. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  260. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  261. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  262. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  263. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  264. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  265. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  266. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  267. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  268. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  269. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  270. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  271. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  272. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  273. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  274. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  275. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  276. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  277. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  278. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  279. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  280. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  281. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  282. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  283. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  284. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  285. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  286. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  287. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  288. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  289. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  290. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  291. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  292. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  293. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  294. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  295. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  296. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  297. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  298. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  299. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  300. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  301. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  302. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  303. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  304. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  305. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  306. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  307. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  308. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  309. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  310. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  311. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  312. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  313. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  314. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  315. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  316. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  317. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  318. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  319. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  320. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  321. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  322. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  323. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  324. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  325. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  326. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  327. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  328. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  329. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  330. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  331. Trial J, et al. 2020. Geroscience. . PubMed
  332. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  333. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  334. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  335. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  336. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  337. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  338. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  339. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  340. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  341. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  342. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  343. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  344. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  345. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  346. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  347. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  348. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  349. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  350. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  351. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  352. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  353. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  354. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  355. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  356. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  357. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  358. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  359. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  360. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  361. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  362. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  363. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  364. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  365. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  366. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  367. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  368. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  369. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  370. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  371. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  372. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  373. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  374. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  375. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  376. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  377. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  378. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  379. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  380. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  381. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  382. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  383. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  384. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  385. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  386. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed

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NA

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