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Ultra-LEAF™ Purified anti-human CD16 Antibody

Pricing & Availability
Clone
W18182B (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
a. W18182B_ULEAF_CD16_FC_1_063035
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) FITC and Ultra-LEAF™ Purified anti-human CD16 (clone W18182B) (left) or purified rat IgG1, κ sotype control (right) followed by anti-rat IgG APC.
  • a. W18182B_ULEAF_CD16_FC_1_063035
    Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) FITC and Ultra-LEAF™ Purified anti-human CD16 (clone W18182B) (left) or purified rat IgG1, κ sotype control (right) followed by anti-rat IgG APC.
  • b. W18182B_ULEAF_CD16_FC_2_063035
    Human peripheral blood mononuclear cells were stimulated with 10 µg/mL plate-bound anti-human CD16 (clone W18182B) ULEAF (filled histogram) or rat IgG1, κ Ultra-LEAF™ isotype control (open histogram). After overnight culture, cells were harvested and stained with anti-human CD107a (clone H4A3) PE. Data shown are gated on the CD3-CD56+ population.
Cat # Size Price Quantity Save
310010 100 µg ¥34,860
310011 1 mg ¥87,980
Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD16 protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Activ - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W18182B can completely block the binding of clone B73.1 on target cells.

Clone W18182B does not affect the binding of clones 3G8 or W18182A on target cells.

Clone W18182B is able to activate NK cells and induce degranulation.

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

Expressed on natural killer cells, activated monocytes, macrophages, neutrophils and a subset of T cells

Function
Low affinity IgG Fc receptor
Ligand/Receptor
IgG Fc (Fcγ)
Cell Type
Granulocytes, Neutrophils, NK cells
Biology Area
Adaptive Immunity, Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711.
  2. Ravetch JV and Bolland S. 2001. Annu Rev Immunol. 19:275.
Gene ID
2214 View all products for this Gene ID 2215 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/30/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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