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Annexin V Binding Buffer Annexin V Binding Buffer

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Other Names
Annexin-V-Binding Buffer
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422201 100 mL NOK297
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Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. 
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  3. Turner JE, et al. 2013. J Exp Med. PubMed
  4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
  8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
  9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
  11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed
Product Citations
  1. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  2. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  3. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  4. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  5. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  6. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  7. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  8. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  9. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  10. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  11. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  14. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  15. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  16. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  17. Vajda F, et al. 2022. Cells. 11:. PubMed
  18. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  19. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  20. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  21. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  22. Quintela M, et al. 2023. Br J Cancer. . PubMed
  23. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  24. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  25. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  26. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  27. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  28. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  29. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  30. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  31. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  32. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  33. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  34. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  35. McNeal AS, et al. 2021. Elife. 10:. PubMed
  36. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  37. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  38. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  39. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  40. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  41. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  42. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  43. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  44. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  45. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  46. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  47. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  48. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  49. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  50. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  51. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  52. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  53. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  54. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  55. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  56. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  57. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  58. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  59. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  60. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  61. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  62. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  63. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  64. Wu J, et al. 2020. Immunity. 53:115. PubMed
  65. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  66. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  67. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  68. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  69. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  70. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  71. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  72. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  73. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  74. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  75. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  76. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  77. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  78. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  79. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  80. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  81. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  82. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  83. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  84. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  85. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  86. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  87. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  88. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  89. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  90. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  91. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  92. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  93. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  94. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  95. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  96. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  97. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  98. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  99. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  100. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  101. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  102. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  103. Harro CM, et al. 2020. J Clin Invest. . PubMed
  104. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  105. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  106. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  107. Dong G, et al. 2020. Haematologica. . PubMed
  108. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  109. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  110. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  111. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  112. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  113. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  114. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  115. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  116. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  117. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  118. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  119. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  120. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  121. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  122. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  123. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  124. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  125. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  126. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  127. Jatho A, et al. 2022. Cells. 11:. PubMed
  128. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  129. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  130. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  131. Vulin M, et al. 2022. Oncogene. :. PubMed
  132. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  133. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  134. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  135. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  136. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  137. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  138. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  139. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  140. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  141. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  142. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  143. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  144. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  145. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  146. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  147. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  148. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  149. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  150. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  151. Vajda F, et al. 2022. Cells. 11:. PubMed
  152. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  153. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  154. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  155. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  156. Quintela M, et al. 2023. Br J Cancer. . PubMed
  157. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  158. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  159. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  160. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  161. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  162. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  163. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  164. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  165. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  166. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  167. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  168. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  169. McNeal AS, et al. 2021. Elife. 10:. PubMed
  170. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  171. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  172. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  173. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  174. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  175. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  176. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  177. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  178. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  179. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  180. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  181. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  182. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  184. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  185. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  186. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  187. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  188. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  189. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  190. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  191. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  192. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  193. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  194. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  195. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  196. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  197. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  198. Wu J, et al. 2020. Immunity. 53:115. PubMed
  199. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  200. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  201. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  202. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  203. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  204. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  205. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  206. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  207. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  208. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  209. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  210. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  211. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  212. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  213. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  214. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  215. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  216. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  217. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  218. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  219. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  220. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  221. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  222. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  223. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  224. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  225. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  226. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  227. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  228. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  229. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  230. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  231. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  232. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  233. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  234. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  235. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  236. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  237. Harro CM, et al. 2020. J Clin Invest. . PubMed
  238. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  239. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  240. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  241. Dong G, et al. 2020. Haematologica. . PubMed
  242. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  243. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  244. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  245. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  246. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  247. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  248. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  249. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  250. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  251. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  252. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  253. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  254. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  255. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  256. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  257. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  258. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  259. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  260. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  261. Jatho A, et al. 2022. Cells. 11:. PubMed
  262. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  263. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  264. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  265. Vulin M, et al. 2022. Oncogene. :. PubMed
  266. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  267. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  268. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed

Antigen Details

Gene ID
NA

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 5    Revision Date: 03.15.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Pricing & Availability
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RUO
Other Names
Annexin-V-Binding Buffer
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422201 100 mL NOK297
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Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. 
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  3. Turner JE, et al. 2013. J Exp Med. PubMed
  4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
  8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
  9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
  11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed
Product Citations
  1. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  2. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  3. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  4. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  5. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  6. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  7. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  8. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  9. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  10. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  11. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  14. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  15. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  16. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  17. Vajda F, et al. 2022. Cells. 11:. PubMed
  18. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  19. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  20. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  21. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  22. Quintela M, et al. 2023. Br J Cancer. . PubMed
  23. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  24. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  25. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  26. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  27. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  28. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  29. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  30. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  31. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  32. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  33. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  34. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  35. McNeal AS, et al. 2021. Elife. 10:. PubMed
  36. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  37. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  38. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  39. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  40. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  41. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  42. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  43. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  44. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  45. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  46. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  47. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  48. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  49. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  50. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  51. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  52. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  53. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  54. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  55. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  56. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  57. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  58. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  59. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  60. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  61. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  62. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  63. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  64. Wu J, et al. 2020. Immunity. 53:115. PubMed
  65. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  66. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  67. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  68. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  69. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  70. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  71. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  72. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  73. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  74. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  75. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  76. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  77. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  78. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  79. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  80. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  81. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  82. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  83. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  84. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  85. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  86. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  87. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  88. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  89. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  90. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  91. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  92. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  93. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  94. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  95. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  96. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  97. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  98. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  99. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  100. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  101. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  102. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  103. Harro CM, et al. 2020. J Clin Invest. . PubMed
  104. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  105. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  106. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  107. Dong G, et al. 2020. Haematologica. . PubMed
  108. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  109. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  110. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  111. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  112. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  113. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  114. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  115. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  116. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  117. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  118. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  119. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  120. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  121. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  122. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  123. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  124. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  125. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  126. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  127. Jatho A, et al. 2022. Cells. 11:. PubMed
  128. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  129. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  130. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  131. Vulin M, et al. 2022. Oncogene. :. PubMed
  132. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  133. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  134. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  135. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  136. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  137. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  138. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  139. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  140. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  141. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  142. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  143. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  144. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  145. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  146. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  147. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  148. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  149. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  150. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  151. Vajda F, et al. 2022. Cells. 11:. PubMed
  152. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  153. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  154. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  155. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  156. Quintela M, et al. 2023. Br J Cancer. . PubMed
  157. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  158. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  159. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  160. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  161. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  162. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  163. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  164. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  165. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  166. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  167. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  168. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  169. McNeal AS, et al. 2021. Elife. 10:. PubMed
  170. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  171. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  172. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  173. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  174. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  175. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  176. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  177. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  178. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  179. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  180. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  181. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  182. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  184. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  185. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  186. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  187. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  188. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  189. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  190. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  191. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  192. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  193. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  194. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  195. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  196. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  197. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  198. Wu J, et al. 2020. Immunity. 53:115. PubMed
  199. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  200. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  201. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  202. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  203. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  204. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  205. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  206. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  207. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  208. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  209. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  210. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  211. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  212. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  213. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  214. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  215. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  216. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  217. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  218. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  219. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  220. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  221. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  222. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  223. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  224. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  225. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  226. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  227. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  228. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  229. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  230. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  231. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  232. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  233. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  234. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  235. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  236. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  237. Harro CM, et al. 2020. J Clin Invest. . PubMed
  238. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  239. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  240. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  241. Dong G, et al. 2020. Haematologica. . PubMed
  242. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  243. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  244. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  245. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  246. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  247. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  248. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  249. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  250. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  251. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  252. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  253. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  254. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  255. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  256. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  257. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  258. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  259. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  260. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  261. Jatho A, et al. 2022. Cells. 11:. PubMed
  262. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  263. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  264. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  265. Vulin M, et al. 2022. Oncogene. :. PubMed
  266. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  267. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  268. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed

Antigen Details

Gene ID
NA

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 5    Revision Date: 03.15.2021

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