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APC anti-mouse CD21/CD35 (CR2/CR1) Antibody APC anti-mouse CD21/CD35 (CR2/CR1) Antibody

Pricing & Availability
Clone
7E9 (See other available formats)
Regulatory Status
RUO
Other Names
CR2/CR1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
7E9_APC_011408
C57BL/6 mouse splenocytes stained with 7E9 APC
  • 7E9_APC_011408
    C57BL/6 mouse splenocytes stained with 7E9 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
123412 100 µg NOK2690
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123411 25 µg NOK982
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Request Bulk Quote
Description

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD35/CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References
  1. Boackle S, et al. 2001 Immunity 15:775.
  2. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
  3. Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
  4. Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Lütge M, et al. 2023. Nat Immunol. . PubMed
  3. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  4. Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed
  5. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  6. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  7. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  8. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  9. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  10. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  11. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  12. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  13. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  14. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  15. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  16. Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed
  17. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  18. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  19. Yunk L, et al. 2009. J Immunol. 183:452. PubMed
  20. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  21. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  22. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  23. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  24. Koenig P, et al. 2014. J Biol Chem. 289:34490. PubMed
  25. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  26. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  27. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  28. Andrés B, et al. 2012. J Immunol. 189:2300. PubMed
  29. Zane N, et al. 2012. J Immunol. 188:3009. PubMed
  30. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  31. Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed
  32. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  33. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  34. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  35. Lütge M, et al. 2023. Nat Immunol. . PubMed
  36. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  37. Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed
  38. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  39. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  40. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  41. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  42. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  43. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  44. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  45. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  46. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  47. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  48. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  49. Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed
  50. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  51. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  52. Yunk L, et al. 2009. J Immunol. 183:452. PubMed
  53. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  54. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  55. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  56. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  57. Koenig P, et al. 2014. J Biol Chem. 289:34490. PubMed
  58. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  59. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  60. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  61. Andrés B, et al. 2012. J Immunol. 189:2300. PubMed
  62. Zane N, et al. 2012. J Immunol. 188:3009. PubMed
  63. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  64. Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed
  65. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  66. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
RRID
AB_2085160 (BioLegend Cat. No. 123412)
AB_940395 (BioLegend Cat. No. 123411)

Antigen Details

Structure
145 kD/190 kD type I transmembrane glycoprotein
Distribution

B cells, follicular dendritic cells

Function
Signal transduction
Ligand/Receptor
Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.

Gene ID
12902 View all products for this Gene ID 12946 View all products for this Gene ID
UniProt
View information about CD21/CD35 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
7E9 (See other available formats)
Regulatory Status
RUO
Other Names
CR2/CR1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
7E9_APC_011408
C57BL/6 mouse splenocytes stained with 7E9 APC
  • 7E9_APC_011408
    C57BL/6 mouse splenocytes stained with 7E9 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
123412 100 µg NOK2690
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123411 25 µg NOK982
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD35/CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References
  1. Boackle S, et al. 2001 Immunity 15:775.
  2. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
  3. Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
  4. Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Lütge M, et al. 2023. Nat Immunol. . PubMed
  3. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  4. Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed
  5. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  6. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  7. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  8. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  9. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  10. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  11. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  12. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  13. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  14. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  15. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  16. Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed
  17. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  18. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  19. Yunk L, et al. 2009. J Immunol. 183:452. PubMed
  20. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  21. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  22. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  23. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  24. Koenig P, et al. 2014. J Biol Chem. 289:34490. PubMed
  25. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  26. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  27. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  28. Andrés B, et al. 2012. J Immunol. 189:2300. PubMed
  29. Zane N, et al. 2012. J Immunol. 188:3009. PubMed
  30. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  31. Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed
  32. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  33. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  34. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  35. Lütge M, et al. 2023. Nat Immunol. . PubMed
  36. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  37. Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed
  38. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  39. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  40. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  41. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  42. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  43. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  44. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  45. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  46. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  47. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  48. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  49. Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed
  50. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  51. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  52. Yunk L, et al. 2009. J Immunol. 183:452. PubMed
  53. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  54. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  55. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  56. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  57. Koenig P, et al. 2014. J Biol Chem. 289:34490. PubMed
  58. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  59. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  60. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  61. Andrés B, et al. 2012. J Immunol. 189:2300. PubMed
  62. Zane N, et al. 2012. J Immunol. 188:3009. PubMed
  63. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  64. Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed
  65. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  66. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
RRID
AB_2085160 (BioLegend Cat. No. 123412)
AB_940395 (BioLegend Cat. No. 123411)

Antigen Details

Structure
145 kD/190 kD type I transmembrane glycoprotein
Distribution

B cells, follicular dendritic cells

Function
Signal transduction
Ligand/Receptor
Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.

Gene ID
12902 View all products for this Gene ID 12946 View all products for this Gene ID
UniProt
View information about CD21/CD35 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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