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APC anti-mouse CD4 Antibody APC anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RM4-5_APC_030206
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • RM4-5_APC_030206
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
100515 25 µg NOK273
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100516 100 µg NOK867
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  2. Casado-García A, et al. 2022. Cancer Res. 82:1098. PubMed
  3. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  4. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  5. Zheng D, et al. 2023. Bioact Mater. 22:567. PubMed
  6. Li J, et al. 2022. Front Immunol. 13:1043484. PubMed
  7. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  8. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  9. Issa N, et al. 2023. Leukemia. :. PubMed
  10. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  11. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  12. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  13. Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed
  14. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  15. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  16. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  17. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  18. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  19. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  20. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  21. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  22. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  23. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  24. Lee J, et al. 2020. Gut Microbes. 1:. PubMed
  25. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  26. Matsumoto M, et al. 2007. J Immunol . 178:2499. PubMed
  27. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  28. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  29. Rodriguez-Manzanet R, et al. 2010. Proc Natl Acad Sci U S A. 107:8706. PubMed
  30. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  31. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  32. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  33. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  34. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  35. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  36. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  37. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  38. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  39. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  40. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  41. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  42. van der Lelie D, et al. 2021. Nat Commun. 12:3105. PubMed
  43. Kang S, et al. 2021. Bio Protoc. 11:e4029. PubMed
  44. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  45. Shigeta A, et al. 2008. Blood. 112:4915. PubMed
  46. Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
  47. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  48. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  49. Park S, et al. 2013. J Immunol. 190:3480. PubMed
  50. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  51. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  52. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  53. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  54. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  55. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  56. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  57. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  58. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  59. Tsumiyama K, et al. 2013. J Immunol. 191:91. PubMed
  60. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  61. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  62. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  63. Biton J, et al. 2016. J Immunol. 197: 1708 - 1719. PubMed
  64. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  65. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  66. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  67. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  68. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  69. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  70. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  71. Liu J, et al. 2021. Front Immunol. 12:733808. PubMed
  72. Xiao u, et al. 2015. J Immunol. 195: 4218 - 4227. PubMed
  73. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  74. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  75. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  76. Zaborsky N, et al. 2010. J Immunol. 184:725. PubMed
  77. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  78. Ene CI, et al. 2020. Neuro Oncol. 639:22. PubMed
  79. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  80. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  81. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  82. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  83. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  84. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  85. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  86. Denzin LK et al. 2017. Immunity. 47(2):310-322 . PubMed
  87. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  88. Sudo K, et al. 2021. Stem Cell Res. 56:102531. PubMed
  89. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  90. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  91. Guo X, et al. 2020. J Cell Mol Med. . PubMed
  92. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  93. Berry G, Waldner H 2013. J Vis Exp. 75: 50389. PubMed
  94. St Clair JR, et al. 2018. Nat Commun. 9:1742. PubMed
  95. Wen X, et al. 2021. World J Gastroenterol. 27:2834. PubMed
  96. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  97. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  98. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  99. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  100. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  101. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  102. García–Rodríguez S, et al. 2018. Sci Rep. 2.664583333. PubMed
  103. Pastor Arroyo EM, et al. 2022. Clin Sci (Lond). 136:557. PubMed
  104. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  105. Palaniyandi S, et al. 2013. PLoS One. 8:61841. PubMed
  106. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  107. Casado-García A, et al. 2022. Cancer Res. 82:1098. PubMed
  108. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  109. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  110. Zheng D, et al. 2023. Bioact Mater. 22:567. PubMed
  111. Li J, et al. 2022. Front Immunol. 13:1043484. PubMed
  112. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  113. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  114. Issa N, et al. 2023. Leukemia. :. PubMed
  115. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  116. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  117. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  118. Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed
  119. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  120. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  121. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  122. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  123. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  124. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  125. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  126. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  127. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  128. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  129. Lee J, et al. 2020. Gut Microbes. 1:. PubMed
  130. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  131. Matsumoto M, et al. 2007. J Immunol . 178:2499. PubMed
  132. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  133. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  134. Rodriguez-Manzanet R, et al. 2010. Proc Natl Acad Sci U S A. 107:8706. PubMed
  135. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  136. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  137. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  138. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  139. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  140. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  141. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  142. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  143. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  144. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  145. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  146. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  147. van der Lelie D, et al. 2021. Nat Commun. 12:3105. PubMed
  148. Kang S, et al. 2021. Bio Protoc. 11:e4029. PubMed
  149. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  150. Shigeta A, et al. 2008. Blood. 112:4915. PubMed
  151. Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
  152. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  153. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  154. Park S, et al. 2013. J Immunol. 190:3480. PubMed
  155. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  156. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  157. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  158. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  159. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  160. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  161. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  162. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  163. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  164. Tsumiyama K, et al. 2013. J Immunol. 191:91. PubMed
  165. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  166. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  167. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  168. Biton J, et al. 2016. J Immunol. 197: 1708 - 1719. PubMed
  169. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  170. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  171. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  172. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  173. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  174. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  175. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  176. Liu J, et al. 2021. Front Immunol. 12:733808. PubMed
  177. Xiao u, et al. 2015. J Immunol. 195: 4218 - 4227. PubMed
  178. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  179. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  180. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  181. Zaborsky N, et al. 2010. J Immunol. 184:725. PubMed
  182. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  183. Ene CI, et al. 2020. Neuro Oncol. 639:22. PubMed
  184. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  185. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  186. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  187. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  188. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  189. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  190. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  191. Denzin LK et al. 2017. Immunity. 47(2):310-322 . PubMed
  192. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  193. Sudo K, et al. 2021. Stem Cell Res. 56:102531. PubMed
  194. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  195. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  196. Guo X, et al. 2020. J Cell Mol Med. . PubMed
  197. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  198. Berry G, Waldner H 2013. J Vis Exp. 75: 50389. PubMed
  199. St Clair JR, et al. 2018. Nat Commun. 9:1742. PubMed
  200. Wen X, et al. 2021. World J Gastroenterol. 27:2834. PubMed
  201. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  202. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  203. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  204. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  205. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  206. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  207. García–Rodríguez S, et al. 2018. Sci Rep. 2.664583333. PubMed
  208. Pastor Arroyo EM, et al. 2022. Clin Sci (Lond). 136:557. PubMed
  209. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  210. Palaniyandi S, et al. 2013. PLoS One. 8:61841. PubMed
RRID
AB_312718 (BioLegend Cat. No. 100515)
AB_312719 (BioLegend Cat. No. 100516)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
PE/Cyanine7 anti-mouse CD4 RM4-5 FC
APC/Cyanine7 anti-mouse CD4 RM4-5 FC
APC/Fire™ 750 anti-mouse CD4 RM4-5 FC
TotalSeq™-A0001 anti-mouse CD4 RM4-5 PG
TotalSeq™-B0001 anti-mouse CD4 RM4-5 PG
TotalSeq™-C0001 anti-mouse CD4 RM4-5 PG
Ultra-LEAF™ Purified anti-mouse CD4 RM4-5 FC,CyTOF®,Depletion,Block,IHC
APC anti-mouse CD4 RM4-5 FC
Biotin anti-mouse CD4 RM4-5 FC
FITC anti-mouse CD4 RM4-5 FC
PE anti-mouse CD4 RM4-5 FC
PE/Cyanine5 anti-mouse CD4 RM4-5 FC
Purified anti-mouse CD4 RM4-5 FC,CyTOF®,IHC-F,IHC
Alexa Fluor® 647 anti-mouse CD4 RM4-5 FC,3D IHC,IHC-F
Alexa Fluor® 488 anti-mouse CD4 RM4-5 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD4 RM4-5 FC
Alexa Fluor® 700 anti-mouse CD4 RM4-5 FC
PerCP anti-mouse CD4 RM4-5 FC
PerCP/Cyanine5.5 anti-mouse CD4 RM4-5 FC
Brilliant Violet 421™ anti-mouse CD4 RM4-5 FC,ICC,IHC-F
Brilliant Violet 570™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 605™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 650™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 711™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 785™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 510™ anti-mouse CD4 RM4-5 FC
Purified anti-mouse CD4 (Maxpar® Ready) RM4-5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD4 RM4-5 FC
Spark Violet™ 423 anti-mouse CD4 RM4-5 FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) RM4-5 FC
APC/Fire™ 810 anti-mouse CD4 RM4-5 FC
PE/Fire™ 640 anti-mouse CD4 Antibody RM4-5 FC
Brilliant Violet 750™ anti-mouse CD4 Antibody RM4-5 FC
Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) RM4-5 FC
StarBright UltraViolet 740 anti-mouse CD4 RM4-5 FC
Go To Top Version: 3    Revision Date: 01.29.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RM4-5_APC_030206
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • RM4-5_APC_030206
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
100515 25 µg NOK273
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100516 100 µg NOK867
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  2. Casado-García A, et al. 2022. Cancer Res. 82:1098. PubMed
  3. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  4. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  5. Zheng D, et al. 2023. Bioact Mater. 22:567. PubMed
  6. Li J, et al. 2022. Front Immunol. 13:1043484. PubMed
  7. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  8. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  9. Issa N, et al. 2023. Leukemia. :. PubMed
  10. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  11. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  12. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  13. Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed
  14. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  15. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  16. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  17. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  18. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  19. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  20. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  21. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  22. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  23. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  24. Lee J, et al. 2020. Gut Microbes. 1:. PubMed
  25. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  26. Matsumoto M, et al. 2007. J Immunol . 178:2499. PubMed
  27. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  28. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  29. Rodriguez-Manzanet R, et al. 2010. Proc Natl Acad Sci U S A. 107:8706. PubMed
  30. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  31. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  32. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  33. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  34. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  35. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  36. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  37. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  38. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  39. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  40. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  41. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  42. van der Lelie D, et al. 2021. Nat Commun. 12:3105. PubMed
  43. Kang S, et al. 2021. Bio Protoc. 11:e4029. PubMed
  44. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  45. Shigeta A, et al. 2008. Blood. 112:4915. PubMed
  46. Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
  47. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  48. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  49. Park S, et al. 2013. J Immunol. 190:3480. PubMed
  50. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  51. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  52. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  53. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  54. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  55. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  56. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  57. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  58. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  59. Tsumiyama K, et al. 2013. J Immunol. 191:91. PubMed
  60. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  61. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  62. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  63. Biton J, et al. 2016. J Immunol. 197: 1708 - 1719. PubMed
  64. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  65. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  66. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  67. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  68. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  69. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  70. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  71. Liu J, et al. 2021. Front Immunol. 12:733808. PubMed
  72. Xiao u, et al. 2015. J Immunol. 195: 4218 - 4227. PubMed
  73. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  74. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  75. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  76. Zaborsky N, et al. 2010. J Immunol. 184:725. PubMed
  77. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  78. Ene CI, et al. 2020. Neuro Oncol. 639:22. PubMed
  79. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  80. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  81. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  82. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  83. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  84. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  85. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  86. Denzin LK et al. 2017. Immunity. 47(2):310-322 . PubMed
  87. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  88. Sudo K, et al. 2021. Stem Cell Res. 56:102531. PubMed
  89. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  90. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  91. Guo X, et al. 2020. J Cell Mol Med. . PubMed
  92. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  93. Berry G, Waldner H 2013. J Vis Exp. 75: 50389. PubMed
  94. St Clair JR, et al. 2018. Nat Commun. 9:1742. PubMed
  95. Wen X, et al. 2021. World J Gastroenterol. 27:2834. PubMed
  96. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  97. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  98. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  99. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  100. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  101. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  102. García–Rodríguez S, et al. 2018. Sci Rep. 2.664583333. PubMed
  103. Pastor Arroyo EM, et al. 2022. Clin Sci (Lond). 136:557. PubMed
  104. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  105. Palaniyandi S, et al. 2013. PLoS One. 8:61841. PubMed
  106. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  107. Casado-García A, et al. 2022. Cancer Res. 82:1098. PubMed
  108. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  109. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  110. Zheng D, et al. 2023. Bioact Mater. 22:567. PubMed
  111. Li J, et al. 2022. Front Immunol. 13:1043484. PubMed
  112. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  113. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  114. Issa N, et al. 2023. Leukemia. :. PubMed
  115. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  116. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  117. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  118. Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed
  119. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  120. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  121. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  122. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  123. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  124. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  125. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  126. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  127. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  128. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  129. Lee J, et al. 2020. Gut Microbes. 1:. PubMed
  130. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  131. Matsumoto M, et al. 2007. J Immunol . 178:2499. PubMed
  132. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  133. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  134. Rodriguez-Manzanet R, et al. 2010. Proc Natl Acad Sci U S A. 107:8706. PubMed
  135. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  136. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  137. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  138. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  139. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  140. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  141. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  142. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  143. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  144. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  145. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  146. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  147. van der Lelie D, et al. 2021. Nat Commun. 12:3105. PubMed
  148. Kang S, et al. 2021. Bio Protoc. 11:e4029. PubMed
  149. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  150. Shigeta A, et al. 2008. Blood. 112:4915. PubMed
  151. Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
  152. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  153. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  154. Park S, et al. 2013. J Immunol. 190:3480. PubMed
  155. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  156. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  157. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  158. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  159. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  160. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  161. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  162. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  163. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  164. Tsumiyama K, et al. 2013. J Immunol. 191:91. PubMed
  165. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  166. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  167. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  168. Biton J, et al. 2016. J Immunol. 197: 1708 - 1719. PubMed
  169. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  170. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  171. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  172. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  173. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  174. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  175. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  176. Liu J, et al. 2021. Front Immunol. 12:733808. PubMed
  177. Xiao u, et al. 2015. J Immunol. 195: 4218 - 4227. PubMed
  178. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  179. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  180. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  181. Zaborsky N, et al. 2010. J Immunol. 184:725. PubMed
  182. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  183. Ene CI, et al. 2020. Neuro Oncol. 639:22. PubMed
  184. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  185. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  186. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  187. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  188. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  189. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  190. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  191. Denzin LK et al. 2017. Immunity. 47(2):310-322 . PubMed
  192. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  193. Sudo K, et al. 2021. Stem Cell Res. 56:102531. PubMed
  194. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  195. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  196. Guo X, et al. 2020. J Cell Mol Med. . PubMed
  197. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  198. Berry G, Waldner H 2013. J Vis Exp. 75: 50389. PubMed
  199. St Clair JR, et al. 2018. Nat Commun. 9:1742. PubMed
  200. Wen X, et al. 2021. World J Gastroenterol. 27:2834. PubMed
  201. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  202. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  203. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  204. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  205. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  206. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  207. García–Rodríguez S, et al. 2018. Sci Rep. 2.664583333. PubMed
  208. Pastor Arroyo EM, et al. 2022. Clin Sci (Lond). 136:557. PubMed
  209. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  210. Palaniyandi S, et al. 2013. PLoS One. 8:61841. PubMed
RRID
AB_312718 (BioLegend Cat. No. 100515)
AB_312719 (BioLegend Cat. No. 100516)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
PE/Cyanine7 anti-mouse CD4 RM4-5 FC
APC/Cyanine7 anti-mouse CD4 RM4-5 FC
APC/Fire™ 750 anti-mouse CD4 RM4-5 FC
TotalSeq™-A0001 anti-mouse CD4 RM4-5 PG
TotalSeq™-B0001 anti-mouse CD4 RM4-5 PG
TotalSeq™-C0001 anti-mouse CD4 RM4-5 PG
Ultra-LEAF™ Purified anti-mouse CD4 RM4-5 FC,CyTOF®,Depletion,Block,IHC
APC anti-mouse CD4 RM4-5 FC
Biotin anti-mouse CD4 RM4-5 FC
FITC anti-mouse CD4 RM4-5 FC
PE anti-mouse CD4 RM4-5 FC
PE/Cyanine5 anti-mouse CD4 RM4-5 FC
Purified anti-mouse CD4 RM4-5 FC,CyTOF®,IHC-F,IHC
Alexa Fluor® 647 anti-mouse CD4 RM4-5 FC,3D IHC,IHC-F
Alexa Fluor® 488 anti-mouse CD4 RM4-5 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD4 RM4-5 FC
Alexa Fluor® 700 anti-mouse CD4 RM4-5 FC
PerCP anti-mouse CD4 RM4-5 FC
PerCP/Cyanine5.5 anti-mouse CD4 RM4-5 FC
Brilliant Violet 421™ anti-mouse CD4 RM4-5 FC,ICC,IHC-F
Brilliant Violet 570™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 605™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 650™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 711™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 785™ anti-mouse CD4 RM4-5 FC
Brilliant Violet 510™ anti-mouse CD4 RM4-5 FC
Purified anti-mouse CD4 (Maxpar® Ready) RM4-5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD4 RM4-5 FC
Spark Violet™ 423 anti-mouse CD4 RM4-5 FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) RM4-5 FC
APC/Fire™ 810 anti-mouse CD4 RM4-5 FC
PE/Fire™ 640 anti-mouse CD4 Antibody RM4-5 FC
Brilliant Violet 750™ anti-mouse CD4 Antibody RM4-5 FC
Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) RM4-5 FC
StarBright UltraViolet 740 anti-mouse CD4 RM4-5 FC
Go To Top Version: 3    Revision Date: 01.29.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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