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APC/Cyanine7 anti-mouse I-A/I-E Antibody APC/Cyanine7 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
    C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
107628 100 µg NOK2409
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107627 25 µg NOK776
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  2. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  3. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  4. Ge W, et al. 2022. Evid Based Complement Alternat Med. 2022:6132289. PubMed
  5. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  6. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  7. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  8. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  9. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  10. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  11. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  12. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  13. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  14. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  15. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  16. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  17. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  18. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  19. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  20. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  21. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  22. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  23. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  24. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  25. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  26. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  27. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  28. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  29. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  30. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  31. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  32. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  33. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  34. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  35. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  36. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  37. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  38. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  39. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  40. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  41. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  42. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  43. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  44. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  45. Acker KP, et al. 2019. iScience. 19:281. PubMed
  46. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  47. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  48. França TT, et al. 2021. JCI Insight. 6:. PubMed
  49. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  50. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  51. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  52. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  53. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  54. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  55. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  56. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  57. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  58. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  59. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  60. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  61. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  62. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  63. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  64. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  65. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  66. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  67. Sung PS, et al. 2022. JCI Insight. :. PubMed
  68. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  69. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  70. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  71. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  72. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  73. Li J, et al. 2019. iScience. 16:468. PubMed
  74. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  75. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  76. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  77. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  78. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  79. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  80. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  81. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  82. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  83. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  84. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  85. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  86. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  87. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  88. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  89. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  90. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  91. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  92. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  93. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  94. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  95. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  96. Deets KA, et al. 2021. Elife. 10:. PubMed
  97. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  98. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  99. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  100. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
  101. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  102. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  103. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  104. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  105. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  106. Ge W, et al. 2022. Evid Based Complement Alternat Med. 2022:6132289. PubMed
  107. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  108. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  109. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  110. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  111. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  112. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  113. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  114. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  115. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  116. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  117. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  118. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  119. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  120. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  121. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  122. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  123. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  124. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  125. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  126. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  127. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  128. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  129. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  130. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  131. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  132. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  133. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  134. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  135. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  136. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  137. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  138. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  139. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  140. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  141. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  142. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  143. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  144. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  145. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  146. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  147. Acker KP, et al. 2019. iScience. 19:281. PubMed
  148. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  149. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  150. França TT, et al. 2021. JCI Insight. 6:. PubMed
  151. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  152. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  153. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  154. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  155. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  156. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  157. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  158. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  159. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  160. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  161. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  162. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  163. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  164. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  165. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  166. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  167. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  168. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  169. Sung PS, et al. 2022. JCI Insight. :. PubMed
  170. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  171. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  172. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  173. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  174. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  175. Li J, et al. 2019. iScience. 16:468. PubMed
  176. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  177. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  178. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  179. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  180. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  181. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  182. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  183. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  184. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  185. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  186. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  187. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  188. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  189. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  190. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  191. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  192. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  193. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  194. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  195. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  196. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  197. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  198. Deets KA, et al. 2021. Elife. 10:. PubMed
  199. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  200. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  201. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  202. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
  203. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  204. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
RRID
AB_2069377 (BioLegend Cat. No. 107628)
AB_1659252 (BioLegend Cat. No. 107627)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 750™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
Spark NIR™ 685 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
StarBright UltraViolet 795 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS B574™ anti-mouse I-A/I-E Antibody M5/114.15.2 FC
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 5    Revision Date: 02.01.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
    C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
107628 100 µg NOK2409
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107627 25 µg NOK776
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  2. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  3. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  4. Ge W, et al. 2022. Evid Based Complement Alternat Med. 2022:6132289. PubMed
  5. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  6. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  7. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  8. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  9. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  10. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  11. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  12. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  13. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  14. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  15. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  16. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  17. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  18. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  19. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  20. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  21. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  22. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  23. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  24. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  25. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  26. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  27. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  28. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  29. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  30. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  31. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  32. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  33. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  34. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  35. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  36. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  37. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  38. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  39. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  40. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  41. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  42. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  43. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  44. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  45. Acker KP, et al. 2019. iScience. 19:281. PubMed
  46. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  47. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  48. França TT, et al. 2021. JCI Insight. 6:. PubMed
  49. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  50. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  51. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  52. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  53. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  54. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  55. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  56. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  57. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  58. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  59. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  60. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  61. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  62. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  63. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  64. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  65. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  66. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  67. Sung PS, et al. 2022. JCI Insight. :. PubMed
  68. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  69. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  70. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  71. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  72. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  73. Li J, et al. 2019. iScience. 16:468. PubMed
  74. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  75. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  76. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  77. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  78. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  79. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  80. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  81. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  82. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  83. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  84. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  85. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  86. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  87. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  88. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  89. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  90. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  91. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  92. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  93. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  94. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  95. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  96. Deets KA, et al. 2021. Elife. 10:. PubMed
  97. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  98. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  99. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  100. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
  101. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  102. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  103. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  104. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  105. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  106. Ge W, et al. 2022. Evid Based Complement Alternat Med. 2022:6132289. PubMed
  107. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  108. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  109. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  110. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  111. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  112. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  113. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  114. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  115. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  116. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  117. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  118. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  119. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  120. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  121. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  122. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  123. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  124. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  125. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  126. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  127. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  128. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  129. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  130. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  131. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  132. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  133. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  134. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  135. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  136. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  137. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  138. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  139. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  140. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  141. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  142. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  143. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  144. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  145. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  146. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  147. Acker KP, et al. 2019. iScience. 19:281. PubMed
  148. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  149. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  150. França TT, et al. 2021. JCI Insight. 6:. PubMed
  151. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  152. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  153. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  154. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  155. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  156. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  157. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  158. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  159. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  160. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  161. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  162. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  163. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  164. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  165. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  166. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  167. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  168. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  169. Sung PS, et al. 2022. JCI Insight. :. PubMed
  170. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  171. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  172. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  173. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  174. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  175. Li J, et al. 2019. iScience. 16:468. PubMed
  176. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  177. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  178. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  179. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  180. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  181. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  182. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  183. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  184. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  185. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  186. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  187. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  188. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  189. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  190. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  191. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  192. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  193. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  194. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  195. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  196. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  197. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  198. Deets KA, et al. 2021. Elife. 10:. PubMed
  199. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  200. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  201. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  202. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
  203. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  204. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
RRID
AB_2069377 (BioLegend Cat. No. 107628)
AB_1659252 (BioLegend Cat. No. 107627)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 750™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
Spark NIR™ 685 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
StarBright UltraViolet 795 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS B574™ anti-mouse I-A/I-E Antibody M5/114.15.2 FC
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 5    Revision Date: 02.01.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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