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PE/Cyanine7 anti-mouse CD19 Antibody PE/Cyanine7 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5_PECyanine7_CD19_Antibody_1_071124
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) FITC and anti-mouse CD19 (clone 6D5) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
  • 6D5_PECyanine7_CD19_Antibody_1_071124
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) FITC and anti-mouse CD19 (clone 6D5) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
115520 100 µg NOK1774
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115519 25 µg NOK636
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  2. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  3. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  4. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  5. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  6. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  7. Friesen M, et al. 2022. Brain Pathol. 32:e13032. PubMed
  8. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  9. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  10. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  11. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  12. Wang Y, et al. 2023. EBioMedicine. 90:104480. PubMed
  13. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  14. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  15. Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed
  16. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  17. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  18. d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed
  19. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  20. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  21. Castañeda-Zegarra S, et al. 2020. Aging (Albany NY). 12:00. PubMed
  22. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  23. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  24. Morgado P, et al. 2014. Infect Immun . 82:4047. PubMed
  25. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  26. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  27. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  28. Lin CA, et al. 2021. Pharmaceutics. 13:. PubMed
  29. Alberti D, et al. 2021. Curr Protoc. 1:e311. PubMed
  30. Rogawski DS, et al. 2021. Nat Commun. 12:2792. PubMed
  31. Ma M, et al. 2021. Front Immunol. 12:718779. PubMed
  32. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  33. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  34. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  35. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  36. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  37. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  38. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  39. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  40. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  41. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  42. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  43. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  44. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  45. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  46. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  47. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  48. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  49. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  50. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  51. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  52. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  53. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  54. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  55. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  56. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  57. Pillonel V, et al. 2016. Sci Rep. 6:37772. PubMed
  58. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  59. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  60. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  61. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  62. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  63. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  64. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  65. Ma K, et al. 2022. iScience. 25:104347. PubMed
  66. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  67. Li M, et al. 2021. Cancers (Basel). 13:. PubMed
  68. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  69. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  70. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  71. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  72. Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
  73. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  74. Ackermann C, et al. 2019. Sci Rep. 9:10624. PubMed
  75. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  76. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  77. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  78. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  79. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  80. Vincent B, et al. 2010. J Immunol. 184:4196. PubMed
  81. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  82. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  83. Korin B, et al. 2020. Sleep. :43. PubMed
  84. Zhang Y, et al. 2022. Front Pharmacol. 13:952775. PubMed
  85. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  86. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  87. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  88. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  89. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  90. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  91. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  92. Song W, et al. 2022. Immunity. 55:290. PubMed
  93. Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed
  94. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
  95. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  96. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
  97. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  98. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  99. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  100. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  101. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  102. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  103. Friesen M, et al. 2022. Brain Pathol. 32:e13032. PubMed
  104. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  105. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  106. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  107. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  108. Wang Y, et al. 2023. EBioMedicine. 90:104480. PubMed
  109. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  110. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  111. Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed
  112. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  113. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  114. d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed
  115. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  116. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  117. Castañeda-Zegarra S, et al. 2020. Aging (Albany NY). 12:00. PubMed
  118. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  119. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  120. Morgado P, et al. 2014. Infect Immun . 82:4047. PubMed
  121. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  122. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  123. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  124. Lin CA, et al. 2021. Pharmaceutics. 13:. PubMed
  125. Alberti D, et al. 2021. Curr Protoc. 1:e311. PubMed
  126. Rogawski DS, et al. 2021. Nat Commun. 12:2792. PubMed
  127. Ma M, et al. 2021. Front Immunol. 12:718779. PubMed
  128. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  129. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  130. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  131. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  132. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  133. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  134. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  135. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  136. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  137. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  138. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  139. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  140. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  141. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  142. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  143. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  144. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  145. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  146. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  147. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  148. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  149. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  150. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  151. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  152. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  153. Pillonel V, et al. 2016. Sci Rep. 6:37772. PubMed
  154. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  155. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  156. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  157. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  158. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  159. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  160. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  161. Ma K, et al. 2022. iScience. 25:104347. PubMed
  162. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  163. Li M, et al. 2021. Cancers (Basel). 13:. PubMed
  164. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  165. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  166. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  167. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  168. Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
  169. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  170. Ackermann C, et al. 2019. Sci Rep. 9:10624. PubMed
  171. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  172. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  173. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  174. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  175. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  176. Vincent B, et al. 2010. J Immunol. 184:4196. PubMed
  177. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  178. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  179. Korin B, et al. 2020. Sleep. :43. PubMed
  180. Zhang Y, et al. 2022. Front Pharmacol. 13:952775. PubMed
  181. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  182. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  183. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  184. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  185. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  186. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  187. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  188. Song W, et al. 2022. Immunity. 55:290. PubMed
  189. Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed
  190. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
  191. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  192. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
RRID
AB_313655 (BioLegend Cat. No. 115520)
AB_313654 (BioLegend Cat. No. 115519)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
PE/Cyanine7 anti-mouse CD19 6D5 FC
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Spark PLUS UV395™ anti-mouse CD19 6D5 FC
Spark Violet™ 538 anti-mouse CD19 6D5 FC
PerCP/Fire™ 780 anti-mouse CD19 Antibody 6D5 FC
PerCP/Fire™ 806 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 593 anti-mouse CD19 (Flexi-Fluor™) Antibody 6D5 FC
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Go To Top Version: 2    Revision Date: 05.24.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5_PECyanine7_CD19_Antibody_1_071124
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) FITC and anti-mouse CD19 (clone 6D5) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
  • 6D5_PECyanine7_CD19_Antibody_1_071124
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) FITC and anti-mouse CD19 (clone 6D5) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
115520 100 µg NOK1774
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115519 25 µg NOK636
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
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  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  2. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  3. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  4. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  5. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  6. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  7. Friesen M, et al. 2022. Brain Pathol. 32:e13032. PubMed
  8. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  9. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  10. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  11. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  12. Wang Y, et al. 2023. EBioMedicine. 90:104480. PubMed
  13. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  14. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  15. Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed
  16. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  17. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  18. d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed
  19. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  20. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  21. Castañeda-Zegarra S, et al. 2020. Aging (Albany NY). 12:00. PubMed
  22. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  23. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  24. Morgado P, et al. 2014. Infect Immun . 82:4047. PubMed
  25. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  26. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  27. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  28. Lin CA, et al. 2021. Pharmaceutics. 13:. PubMed
  29. Alberti D, et al. 2021. Curr Protoc. 1:e311. PubMed
  30. Rogawski DS, et al. 2021. Nat Commun. 12:2792. PubMed
  31. Ma M, et al. 2021. Front Immunol. 12:718779. PubMed
  32. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  33. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  34. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  35. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  36. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  37. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  38. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  39. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  40. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  41. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  42. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  43. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  44. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  45. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  46. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  47. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  48. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  49. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  50. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  51. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  52. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  53. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  54. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  55. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  56. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  57. Pillonel V, et al. 2016. Sci Rep. 6:37772. PubMed
  58. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  59. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  60. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  61. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  62. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  63. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  64. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  65. Ma K, et al. 2022. iScience. 25:104347. PubMed
  66. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
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  76. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
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  80. Vincent B, et al. 2010. J Immunol. 184:4196. PubMed
  81. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
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  88. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
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  91. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
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  93. Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed
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  95. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  96. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
  97. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  98. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  99. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  100. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  101. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  102. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  103. Friesen M, et al. 2022. Brain Pathol. 32:e13032. PubMed
  104. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  105. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  106. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  107. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  108. Wang Y, et al. 2023. EBioMedicine. 90:104480. PubMed
  109. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  110. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  111. Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed
  112. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  113. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  114. d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed
  115. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  116. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  117. Castañeda-Zegarra S, et al. 2020. Aging (Albany NY). 12:00. PubMed
  118. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  119. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  120. Morgado P, et al. 2014. Infect Immun . 82:4047. PubMed
  121. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  122. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  123. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  124. Lin CA, et al. 2021. Pharmaceutics. 13:. PubMed
  125. Alberti D, et al. 2021. Curr Protoc. 1:e311. PubMed
  126. Rogawski DS, et al. 2021. Nat Commun. 12:2792. PubMed
  127. Ma M, et al. 2021. Front Immunol. 12:718779. PubMed
  128. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  129. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  130. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  131. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  132. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  133. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  134. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  135. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  136. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  137. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  138. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  139. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  140. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  141. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  142. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  143. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  144. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  145. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  146. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  147. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  148. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  149. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  150. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  151. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  152. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  153. Pillonel V, et al. 2016. Sci Rep. 6:37772. PubMed
  154. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  155. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  156. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  157. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  158. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  159. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  160. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  161. Ma K, et al. 2022. iScience. 25:104347. PubMed
  162. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  163. Li M, et al. 2021. Cancers (Basel). 13:. PubMed
  164. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  165. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  166. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  167. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  168. Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
  169. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  170. Ackermann C, et al. 2019. Sci Rep. 9:10624. PubMed
  171. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  172. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  173. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  174. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  175. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  176. Vincent B, et al. 2010. J Immunol. 184:4196. PubMed
  177. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  178. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  179. Korin B, et al. 2020. Sleep. :43. PubMed
  180. Zhang Y, et al. 2022. Front Pharmacol. 13:952775. PubMed
  181. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  182. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  183. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  184. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  185. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  186. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  187. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  188. Song W, et al. 2022. Immunity. 55:290. PubMed
  189. Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed
  190. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
  191. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  192. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
RRID
AB_313655 (BioLegend Cat. No. 115520)
AB_313654 (BioLegend Cat. No. 115519)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
PE/Cyanine7 anti-mouse CD19 6D5 FC
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Spark PLUS UV395™ anti-mouse CD19 6D5 FC
Spark Violet™ 538 anti-mouse CD19 6D5 FC
PerCP/Fire™ 780 anti-mouse CD19 Antibody 6D5 FC
PerCP/Fire™ 806 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 593 anti-mouse CD19 (Flexi-Fluor™) Antibody 6D5 FC
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Go To Top Version: 2    Revision Date: 05.24.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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