PE Rat IgG2a, κ Isotype Ctrl Antibody PE Rat IgG2a, κ Isotype Ctrl Antibody

Product Details

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Trinitrophenol + KLH

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References

1. Nishimoto H, et al. 2005. Blood 106:4241.
2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)

Product Citations

1. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
2. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
3. Malolina EA, et al. 2022. Mol Reprod Dev. 89:243. PubMed
4. Bommireddy R, et al. 2022. Vaccines (Basel). 10: . PubMed
5. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
6. Contreras O, et al. 2023. Front Cell Dev Biol. 11:1017660. PubMed
7. Dona MSI, et al. 2023. Basic Res Cardiol. 118:11. PubMed
8. Kase N, et al. 2023. Front Cell Dev Biol. 11:1022081. PubMed
9. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
10. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
11. Barkaway A, et al. 2021. Immunity. . PubMed
12. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
13. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
14. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
15. Madera L, et al. 2015. PLoS One. 10: 0133385. PubMed
16. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
17. Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed
18. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
19. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
20. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
21. Zape J, et al. 2017. Cell Cycle. 10.1080/15384101.2017.1361569. PubMed
22. Hauptmann M, et al. 2017. Infect Immun . 10.1128/IAI.00156-17. PubMed
23. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
24. Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
25. Toda S, et al. 2014. Blood. 123:3963. PubMed
26. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
27. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
28. Cardot-Ruffino V, et al. 2020. Genesis. 58:e23359. PubMed
29. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
30. Kovacs L, et al. 2020. PLoS One. 15:e0240455. PubMed
31. Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed
32. Chung H, et al. 2021. Nat Methods. 18:1204. PubMed
33. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
34. Takayanagi Y, et al. 2020. J Clin Med. 9:00. PubMed
35. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
36. Kubiniok P, et al. 2019. Mol Cell Proteomics. 18:408. PubMed
37. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
38. Cardot-Ruffino V, et al. 2020. Sci Rep. 10:3880. PubMed
39. Sakurai T, et al. 2021. Life Sci Alliance. 4:. PubMed
40. Yang H, et al. 2022. Nutrients. 14:. PubMed
41. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
42. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
43. Chou PR, et al. 2020. Int J Med Sci. 17:354. PubMed
44. Pereira M, et al. 2022. Cell Rep. 40:111225. PubMed
45. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
46. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
47. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
48. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
49. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
50. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
51. Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed
52. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
53. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
54. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
55. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
56. Rajashekara G, et al. 2008. J Bacteriol. 190:6243. PubMed
57. Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed
58. Yang Z, et al. 2020. J Invest Dermatol. 544:140. PubMed
59. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
60. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
61. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
62. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
63. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
64. Grisanti LA, et al. 2019. JCI Insight. 5. PubMed
65. Sockolosky J, et al. 2016. Proc Natl Acad Sci U S A. 113: 2646 - 2654. PubMed
66. Adamopoulos I, et al. 2015. Ann Rheum Dis. 74:1284. PubMed
67. Schmidt N, et al. 2011. J Immunol. 186:5873. PubMed
68. Arya SB, et al. 2018. J Biol Chem. 293:9892. PubMed
69. Asson-Batres M, et al. 2016. Am J Physiol Heart Circ Physiol. 310: 1773 - 1789. PubMed
70. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
71. Li ZG, et al. 2018. Mil Med Res. 5:24. PubMed
72. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
73. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
74. Kawamoto E, et al. 2021. Biomedicines. 9:. PubMed
75. Harada Y, et al. 2019. Pain. 160:2050. PubMed
76. Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
77. Wu KKL, et al. 2021. Nat Commun. 12:6637. PubMed
78. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
79. Nott A, et al. 2021. Nature Protocols. 16(3):1629-1646. PubMed
80. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
81. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
82. Malolina EA, et al. 2022. Mol Reprod Dev. 89:243. PubMed
83. Bommireddy R, et al. 2022. Vaccines (Basel). 10: . PubMed
84. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
85. Contreras O, et al. 2023. Front Cell Dev Biol. 11:1017660. PubMed
86. Dona MSI, et al. 2023. Basic Res Cardiol. 118:11. PubMed
87. Kase N, et al. 2023. Front Cell Dev Biol. 11:1022081. PubMed
88. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
89. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
90. Barkaway A, et al. 2021. Immunity. . PubMed
91. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
92. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
93. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
94. Madera L, et al. 2015. PLoS One. 10: 0133385. PubMed
95. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
96. Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed
97. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
98. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
99. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
100. Zape J, et al. 2017. Cell Cycle. 10.1080/15384101.2017.1361569. PubMed
101. Hauptmann M, et al. 2017. Infect Immun . 10.1128/IAI.00156-17. PubMed
102. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
103. Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
104. Toda S, et al. 2014. Blood. 123:3963. PubMed
105. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
106. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
107. Cardot-Ruffino V, et al. 2020. Genesis. 58:e23359. PubMed
108. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
109. Kovacs L, et al. 2020. PLoS One. 15:e0240455. PubMed
110. Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed
111. Chung H, et al. 2021. Nat Methods. 18:1204. PubMed
112. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
113. Takayanagi Y, et al. 2020. J Clin Med. 9:00. PubMed
114. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
115. Kubiniok P, et al. 2019. Mol Cell Proteomics. 18:408. PubMed
116. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
117. Cardot-Ruffino V, et al. 2020. Sci Rep. 10:3880. PubMed
118. Sakurai T, et al. 2021. Life Sci Alliance. 4:. PubMed
119. Yang H, et al. 2022. Nutrients. 14:. PubMed
120. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
121. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
122. Chou PR, et al. 2020. Int J Med Sci. 17:354. PubMed
123. Pereira M, et al. 2022. Cell Rep. 40:111225. PubMed
124. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
125. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
126. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
127. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
128. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
129. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
130. Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed
131. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
132. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
133. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
134. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
135. Rajashekara G, et al. 2008. J Bacteriol. 190:6243. PubMed
136. Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed
137. Yang Z, et al. 2020. J Invest Dermatol. 544:140. PubMed
138. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
139. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
140. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
141. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
142. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
143. Grisanti LA, et al. 2019. JCI Insight. 5. PubMed
144. Sockolosky J, et al. 2016. Proc Natl Acad Sci U S A. 113: 2646 - 2654. PubMed
145. Adamopoulos I, et al. 2015. Ann Rheum Dis. 74:1284. PubMed
146. Schmidt N, et al. 2011. J Immunol. 186:5873. PubMed
147. Arya SB, et al. 2018. J Biol Chem. 293:9892. PubMed
148. Asson-Batres M, et al. 2016. Am J Physiol Heart Circ Physiol. 310: 1773 - 1789. PubMed
149. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
150. Li ZG, et al. 2018. Mil Med Res. 5:24. PubMed
151. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
152. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
153. Kawamoto E, et al. 2021. Biomedicines. 9:. PubMed
154. Harada Y, et al. 2019. Pain. 160:2050. PubMed
155. Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
156. Wu KKL, et al. 2021. Nat Commun. 12:6637. PubMed
157. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
158. Nott A, et al. 2021. Nature Protocols. 16(3):1629-1646. PubMed

RRID

AB_326530 (BioLegend Cat. No. 400507)
AB_326530 (BioLegend Cat. No. 400508)

Antigen Details

Gene ID

NA

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Certificate of Analysis