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Purified anti-mouse CD4 Antibody Purified anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
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Product Citations
publications
GK1dot5_Pure_021706
BALB/c mouse splenocytes were stained with purified CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC.
  • GK1dot5_Pure_021706
    BALB/c mouse splenocytes were stained with purified CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC.
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100401 50 µg NOK198
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100402 500 µg NOK636
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  2. Chami B, et al. 2017. Sci Rep. 7:10181. PubMed
  3. Tsukumo S, et al. 2006. J Immunol. 177:8365. PubMed
  4. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  5. Liao X, et al. 2022. Front Immunol. 12:768813. PubMed
  6. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  7. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  8. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  9. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  10. Ngwenyama N, et al. 2022. Nat Cardiovasc Res. 1:761. PubMed
  11. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  12. Smith CJ, et al. 2018. J Immunol. 200:1580. PubMed
  13. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  14. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  15. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  16. Thi VAD, et al. 2019. Mol Cells. 42:869. PubMed
  17. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  18. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  19. Hummel JF, et al. 2020. Mucosal Immunol. 0.720138889. PubMed
  20. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  21. Fernandes V, et al. 2020. PLoS Pathog. 16:e1008464. PubMed
  22. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  23. Murata A, et al. 2020. Front Immunol. 11:775. PubMed
  24. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  25. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  26. Duvvuri M, et al. 2019. Biomater Sci. 7:1863. PubMed
  27. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
  28. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  29. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  30. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  31. Hashimoto K, et al. 2021. J Dermatol Sci. 102:177. PubMed
  32. Haque M, et al. 2021. Viruses. 13:. PubMed
  33. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  34. Tian K, et al. 2021. J Immunol Res. 2021:6297332. PubMed
  35. Hirano J, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  36. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
  37. Rossi DC, et al. 2021. J Clin Invest. 131:. PubMed
  38. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  39. Xie MM, et al. 2017. Eur J Immunol. 47:1136. PubMed
  40. Zhou AC, et al. 2017. Front Immunol. 1.136111111. PubMed
  41. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  42. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  43. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  44. Mostafavi H, et al. 2022. PLoS Pathog. 18:e1010185. PubMed
  45. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  46. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  47. Jackson JW, et al. 2021. Mol Ther Oncolytics. 22:444. PubMed
  48. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  49. Lasrado N, et al. 2021. Microorganisms. 9:. PubMed
  50. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  51. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  52. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  53. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  54. Wang N, et al. 2021. Mol Ther Oncolytics. 20:71. PubMed
  55. Skeate JG, et al. 2018. PLoS One. 13:e0191311. PubMed
  56. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  57. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  58. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  59. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  60. Sharma M, et al. 2020. Nat Commun. 11:661. PubMed
  61. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  62. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  63. Elsayed H, et al. 2018. J Virol. 92:e00428. PubMed
  64. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  65. Hu M, et al. 2008. J Immunol. 180:6593. PubMed
  66. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  67. Shokirova H, et al. 2021. Sci Rep. 11:8647. PubMed
  68. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  69. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  70. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  71. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  72. Horiuchi S, et al. 2021. Sci Immunol. :eabm3131. PubMed
  73. Pierini A, et al. 2017. JCI Insight. 2. PubMed
  74. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  75. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  76. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  77. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  78. Majer O, et al. 2019. Nature. 575:366. PubMed
  79. Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
  80. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  81. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
  82. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  83. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  84. Zhang H, et al. 2017. J Immunol. 198:2133. PubMed
  85. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  86. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  87. Zander R, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.077. PubMed
  88. Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
  89. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  90. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  91. Ikezoe T, et al. 2021. Biomolecules. 11:. PubMed
  92. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  93. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  94. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  95. Meyers JL, et al. 2018. PLoS One. 13:e0207007. PubMed
  96. Floudas A, et al. 2017. J Immunol. 199:707. PubMed
  97. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  98. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  99. Pradhan P, et al. 2017. JCI Insight. 2. PubMed
  100. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  101. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  102. Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
  103. Wilhelmson AS, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1519. PubMed
  104. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  105. Stefanski HE, et al. 2018. PLoS One. 13:e0198871. PubMed
  106. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  107. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  108. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  109. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  110. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  111. Zhang Z, et al. 2020. Front Immunol. 1.844444444. PubMed
  112. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  113. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  114. Luu M, et al. 2019. Nat Commun. 10:760. PubMed
  115. Di Dalmazi G, et al. 2019. J Immunol. 202:1350. PubMed
  116. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  117. Dey P, et al. 2020. Cancer Discov. 10:608. PubMed
  118. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  119. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  120. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed
  121. Fujimura N, et al. 2016. J Vasc Surg. 64: 46-54. PubMed
  122. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  123. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  124. Zhang N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  125. Whittle JR, et al. 2020. Clin Cancer Res. 26:4120. PubMed
  126. Liu H, et al. 2022. Animal Model Exp Med. 5:72. PubMed
  127. Krämer TJ, et al. 2019. J Neuroinflammation. 16:163. PubMed
  128. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  129. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
  130. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  131. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
  132. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
  133. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  134. Chami B, et al. 2017. Sci Rep. 7:10181. PubMed
  135. Tsukumo S, et al. 2006. J Immunol. 177:8365. PubMed
  136. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  137. Liao X, et al. 2022. Front Immunol. 12:768813. PubMed
  138. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  139. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  140. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  141. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  142. Ngwenyama N, et al. 2022. Nat Cardiovasc Res. 1:761. PubMed
  143. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  144. Smith CJ, et al. 2018. J Immunol. 200:1580. PubMed
  145. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  146. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  147. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  148. Thi VAD, et al. 2019. Mol Cells. 42:869. PubMed
  149. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  150. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  151. Hummel JF, et al. 2020. Mucosal Immunol. 0.720138889. PubMed
  152. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  153. Fernandes V, et al. 2020. PLoS Pathog. 16:e1008464. PubMed
  154. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  155. Murata A, et al. 2020. Front Immunol. 11:775. PubMed
  156. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  157. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  158. Duvvuri M, et al. 2019. Biomater Sci. 7:1863. PubMed
  159. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
  160. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  161. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  162. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  163. Hashimoto K, et al. 2021. J Dermatol Sci. 102:177. PubMed
  164. Haque M, et al. 2021. Viruses. 13:. PubMed
  165. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  166. Tian K, et al. 2021. J Immunol Res. 2021:6297332. PubMed
  167. Hirano J, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  168. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
  169. Rossi DC, et al. 2021. J Clin Invest. 131:. PubMed
  170. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  171. Xie MM, et al. 2017. Eur J Immunol. 47:1136. PubMed
  172. Zhou AC, et al. 2017. Front Immunol. 1.136111111. PubMed
  173. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  174. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  175. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  176. Mostafavi H, et al. 2022. PLoS Pathog. 18:e1010185. PubMed
  177. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  178. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  179. Jackson JW, et al. 2021. Mol Ther Oncolytics. 22:444. PubMed
  180. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  181. Lasrado N, et al. 2021. Microorganisms. 9:. PubMed
  182. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  183. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  184. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  185. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  186. Wang N, et al. 2021. Mol Ther Oncolytics. 20:71. PubMed
  187. Skeate JG, et al. 2018. PLoS One. 13:e0191311. PubMed
  188. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  189. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  190. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  191. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  192. Sharma M, et al. 2020. Nat Commun. 11:661. PubMed
  193. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  194. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  195. Elsayed H, et al. 2018. J Virol. 92:e00428. PubMed
  196. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  197. Hu M, et al. 2008. J Immunol. 180:6593. PubMed
  198. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  199. Shokirova H, et al. 2021. Sci Rep. 11:8647. PubMed
  200. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  201. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  202. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  203. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  204. Horiuchi S, et al. 2021. Sci Immunol. :eabm3131. PubMed
  205. Pierini A, et al. 2017. JCI Insight. 2. PubMed
  206. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  207. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  208. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  209. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  210. Majer O, et al. 2019. Nature. 575:366. PubMed
  211. Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
  212. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  213. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
  214. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  215. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  216. Zhang H, et al. 2017. J Immunol. 198:2133. PubMed
  217. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  218. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  219. Zander R, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.077. PubMed
  220. Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
  221. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  222. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  223. Ikezoe T, et al. 2021. Biomolecules. 11:. PubMed
  224. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  225. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  226. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  227. Meyers JL, et al. 2018. PLoS One. 13:e0207007. PubMed
  228. Floudas A, et al. 2017. J Immunol. 199:707. PubMed
  229. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  230. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  231. Pradhan P, et al. 2017. JCI Insight. 2. PubMed
  232. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  233. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  234. Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
  235. Wilhelmson AS, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1519. PubMed
  236. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  237. Stefanski HE, et al. 2018. PLoS One. 13:e0198871. PubMed
  238. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  239. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  240. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  241. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  242. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  243. Zhang Z, et al. 2020. Front Immunol. 1.844444444. PubMed
  244. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  245. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  246. Luu M, et al. 2019. Nat Commun. 10:760. PubMed
  247. Di Dalmazi G, et al. 2019. J Immunol. 202:1350. PubMed
  248. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  249. Dey P, et al. 2020. Cancer Discov. 10:608. PubMed
  250. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  251. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  252. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed
  253. Fujimura N, et al. 2016. J Vasc Surg. 64: 46-54. PubMed
  254. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  255. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  256. Zhang N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  257. Whittle JR, et al. 2020. Clin Cancer Res. 26:4120. PubMed
  258. Liu H, et al. 2022. Animal Model Exp Med. 5:72. PubMed
  259. Krämer TJ, et al. 2019. J Neuroinflammation. 16:163. PubMed
  260. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  261. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
  262. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  263. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
  264. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
RRID
AB_312686 (BioLegend Cat. No. 100401)
AB_312687 (BioLegend Cat. No. 100402)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
PE/Cyanine7 anti-mouse CD4 GK1.5 FC
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC,IHC-F,ICC
FITC anti-mouse CD4 GK1.5 FC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC,IHC-F,ICC,IP,Costim,Block,Depletion
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC,IHC-F
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC,IHC-F
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F,FC,SB
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Spark Blue™ 515 anti-mouse CD4 GK1.5 FC
Spark PLUS UV395™ anti-mouse CD4 GK1.5 FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
PerCP/Fire™ 806 anti-mouse CD4 Antibody GK1.5 FC
PerCP/Fire™ 780 anti-mouse CD4 Antibody GK1.5 FC
Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
Spark PLUS V475™ anti-mouse CD4 GK1.5 FC
StarBright UltraViolet 795 anti-mouse CD4 GK1.5 FC
Spark PLUS B488™ anti-mouse CD4 GK1.5 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
GK1dot5_Pure_021706
BALB/c mouse splenocytes were stained with purified CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC.
  • GK1dot5_Pure_021706
    BALB/c mouse splenocytes were stained with purified CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC.
Compare all formats
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100401 50 µg NOK198
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100402 500 µg NOK636
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  2. Chami B, et al. 2017. Sci Rep. 7:10181. PubMed
  3. Tsukumo S, et al. 2006. J Immunol. 177:8365. PubMed
  4. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  5. Liao X, et al. 2022. Front Immunol. 12:768813. PubMed
  6. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  7. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  8. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  9. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  10. Ngwenyama N, et al. 2022. Nat Cardiovasc Res. 1:761. PubMed
  11. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  12. Smith CJ, et al. 2018. J Immunol. 200:1580. PubMed
  13. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  14. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  15. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  16. Thi VAD, et al. 2019. Mol Cells. 42:869. PubMed
  17. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  18. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  19. Hummel JF, et al. 2020. Mucosal Immunol. 0.720138889. PubMed
  20. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  21. Fernandes V, et al. 2020. PLoS Pathog. 16:e1008464. PubMed
  22. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  23. Murata A, et al. 2020. Front Immunol. 11:775. PubMed
  24. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  25. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  26. Duvvuri M, et al. 2019. Biomater Sci. 7:1863. PubMed
  27. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
  28. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  29. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  30. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  31. Hashimoto K, et al. 2021. J Dermatol Sci. 102:177. PubMed
  32. Haque M, et al. 2021. Viruses. 13:. PubMed
  33. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  34. Tian K, et al. 2021. J Immunol Res. 2021:6297332. PubMed
  35. Hirano J, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  36. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
  37. Rossi DC, et al. 2021. J Clin Invest. 131:. PubMed
  38. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  39. Xie MM, et al. 2017. Eur J Immunol. 47:1136. PubMed
  40. Zhou AC, et al. 2017. Front Immunol. 1.136111111. PubMed
  41. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  42. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  43. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  44. Mostafavi H, et al. 2022. PLoS Pathog. 18:e1010185. PubMed
  45. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  46. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  47. Jackson JW, et al. 2021. Mol Ther Oncolytics. 22:444. PubMed
  48. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  49. Lasrado N, et al. 2021. Microorganisms. 9:. PubMed
  50. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  51. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  52. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  53. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  54. Wang N, et al. 2021. Mol Ther Oncolytics. 20:71. PubMed
  55. Skeate JG, et al. 2018. PLoS One. 13:e0191311. PubMed
  56. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  57. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  58. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  59. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  60. Sharma M, et al. 2020. Nat Commun. 11:661. PubMed
  61. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  62. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  63. Elsayed H, et al. 2018. J Virol. 92:e00428. PubMed
  64. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  65. Hu M, et al. 2008. J Immunol. 180:6593. PubMed
  66. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  67. Shokirova H, et al. 2021. Sci Rep. 11:8647. PubMed
  68. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  69. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  70. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  71. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  72. Horiuchi S, et al. 2021. Sci Immunol. :eabm3131. PubMed
  73. Pierini A, et al. 2017. JCI Insight. 2. PubMed
  74. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  75. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  76. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  77. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  78. Majer O, et al. 2019. Nature. 575:366. PubMed
  79. Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
  80. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  81. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
  82. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  83. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  84. Zhang H, et al. 2017. J Immunol. 198:2133. PubMed
  85. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  86. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  87. Zander R, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.077. PubMed
  88. Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
  89. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  90. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  91. Ikezoe T, et al. 2021. Biomolecules. 11:. PubMed
  92. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  93. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  94. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  95. Meyers JL, et al. 2018. PLoS One. 13:e0207007. PubMed
  96. Floudas A, et al. 2017. J Immunol. 199:707. PubMed
  97. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  98. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  99. Pradhan P, et al. 2017. JCI Insight. 2. PubMed
  100. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  101. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  102. Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
  103. Wilhelmson AS, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1519. PubMed
  104. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  105. Stefanski HE, et al. 2018. PLoS One. 13:e0198871. PubMed
  106. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  107. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  108. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  109. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  110. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  111. Zhang Z, et al. 2020. Front Immunol. 1.844444444. PubMed
  112. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  113. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  114. Luu M, et al. 2019. Nat Commun. 10:760. PubMed
  115. Di Dalmazi G, et al. 2019. J Immunol. 202:1350. PubMed
  116. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  117. Dey P, et al. 2020. Cancer Discov. 10:608. PubMed
  118. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  119. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  120. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed
  121. Fujimura N, et al. 2016. J Vasc Surg. 64: 46-54. PubMed
  122. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  123. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  124. Zhang N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  125. Whittle JR, et al. 2020. Clin Cancer Res. 26:4120. PubMed
  126. Liu H, et al. 2022. Animal Model Exp Med. 5:72. PubMed
  127. Krämer TJ, et al. 2019. J Neuroinflammation. 16:163. PubMed
  128. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  129. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
  130. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  131. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
  132. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
  133. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  134. Chami B, et al. 2017. Sci Rep. 7:10181. PubMed
  135. Tsukumo S, et al. 2006. J Immunol. 177:8365. PubMed
  136. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  137. Liao X, et al. 2022. Front Immunol. 12:768813. PubMed
  138. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  139. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  140. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  141. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  142. Ngwenyama N, et al. 2022. Nat Cardiovasc Res. 1:761. PubMed
  143. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  144. Smith CJ, et al. 2018. J Immunol. 200:1580. PubMed
  145. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  146. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  147. Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
  148. Thi VAD, et al. 2019. Mol Cells. 42:869. PubMed
  149. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  150. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  151. Hummel JF, et al. 2020. Mucosal Immunol. 0.720138889. PubMed
  152. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  153. Fernandes V, et al. 2020. PLoS Pathog. 16:e1008464. PubMed
  154. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  155. Murata A, et al. 2020. Front Immunol. 11:775. PubMed
  156. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  157. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  158. Duvvuri M, et al. 2019. Biomater Sci. 7:1863. PubMed
  159. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
  160. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  161. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  162. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  163. Hashimoto K, et al. 2021. J Dermatol Sci. 102:177. PubMed
  164. Haque M, et al. 2021. Viruses. 13:. PubMed
  165. Mayer JU, et al. 2020. Front Immunol. 11:592325. PubMed
  166. Tian K, et al. 2021. J Immunol Res. 2021:6297332. PubMed
  167. Hirano J, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  168. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
  169. Rossi DC, et al. 2021. J Clin Invest. 131:. PubMed
  170. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  171. Xie MM, et al. 2017. Eur J Immunol. 47:1136. PubMed
  172. Zhou AC, et al. 2017. Front Immunol. 1.136111111. PubMed
  173. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  174. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  175. Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed
  176. Mostafavi H, et al. 2022. PLoS Pathog. 18:e1010185. PubMed
  177. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  178. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  179. Jackson JW, et al. 2021. Mol Ther Oncolytics. 22:444. PubMed
  180. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  181. Lasrado N, et al. 2021. Microorganisms. 9:. PubMed
  182. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  183. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  184. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  185. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  186. Wang N, et al. 2021. Mol Ther Oncolytics. 20:71. PubMed
  187. Skeate JG, et al. 2018. PLoS One. 13:e0191311. PubMed
  188. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  189. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  190. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  191. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  192. Sharma M, et al. 2020. Nat Commun. 11:661. PubMed
  193. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  194. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  195. Elsayed H, et al. 2018. J Virol. 92:e00428. PubMed
  196. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  197. Hu M, et al. 2008. J Immunol. 180:6593. PubMed
  198. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  199. Shokirova H, et al. 2021. Sci Rep. 11:8647. PubMed
  200. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  201. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  202. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  203. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  204. Horiuchi S, et al. 2021. Sci Immunol. :eabm3131. PubMed
  205. Pierini A, et al. 2017. JCI Insight. 2. PubMed
  206. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  207. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  208. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  209. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  210. Majer O, et al. 2019. Nature. 575:366. PubMed
  211. Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
  212. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  213. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
  214. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  215. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  216. Zhang H, et al. 2017. J Immunol. 198:2133. PubMed
  217. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  218. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  219. Zander R, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.077. PubMed
  220. Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
  221. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  222. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  223. Ikezoe T, et al. 2021. Biomolecules. 11:. PubMed
  224. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  225. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  226. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  227. Meyers JL, et al. 2018. PLoS One. 13:e0207007. PubMed
  228. Floudas A, et al. 2017. J Immunol. 199:707. PubMed
  229. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  230. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  231. Pradhan P, et al. 2017. JCI Insight. 2. PubMed
  232. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  233. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  234. Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
  235. Wilhelmson AS, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1519. PubMed
  236. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  237. Stefanski HE, et al. 2018. PLoS One. 13:e0198871. PubMed
  238. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  239. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  240. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  241. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  242. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  243. Zhang Z, et al. 2020. Front Immunol. 1.844444444. PubMed
  244. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  245. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
  246. Luu M, et al. 2019. Nat Commun. 10:760. PubMed
  247. Di Dalmazi G, et al. 2019. J Immunol. 202:1350. PubMed
  248. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  249. Dey P, et al. 2020. Cancer Discov. 10:608. PubMed
  250. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  251. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  252. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed
  253. Fujimura N, et al. 2016. J Vasc Surg. 64: 46-54. PubMed
  254. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  255. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  256. Zhang N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  257. Whittle JR, et al. 2020. Clin Cancer Res. 26:4120. PubMed
  258. Liu H, et al. 2022. Animal Model Exp Med. 5:72. PubMed
  259. Krämer TJ, et al. 2019. J Neuroinflammation. 16:163. PubMed
  260. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  261. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
  262. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  263. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
  264. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
RRID
AB_312686 (BioLegend Cat. No. 100401)
AB_312687 (BioLegend Cat. No. 100402)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
PE/Cyanine7 anti-mouse CD4 GK1.5 FC
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC,IHC-F,ICC
FITC anti-mouse CD4 GK1.5 FC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC,IHC-F,ICC,IP,Costim,Block,Depletion
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC,IHC-F
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC,IHC-F
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F,FC,SB
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Spark Blue™ 515 anti-mouse CD4 GK1.5 FC
Spark PLUS UV395™ anti-mouse CD4 GK1.5 FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
PerCP/Fire™ 806 anti-mouse CD4 Antibody GK1.5 FC
PerCP/Fire™ 780 anti-mouse CD4 Antibody GK1.5 FC
Spark YG™ 581 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
Spark PLUS V475™ anti-mouse CD4 GK1.5 FC
StarBright UltraViolet 795 anti-mouse CD4 GK1.5 FC
Spark PLUS B488™ anti-mouse CD4 GK1.5 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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