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Purified anti-Rb Phospho (Ser795) Antibody Purified anti-Rb Phospho (Ser795) Antibody

Pricing & Availability
Clone
A24001E (See other available formats)
Regulatory Status
RUO
Other Names
RB1, Retinoblastoma-associated protein, p105-Rb, p110-RB1
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
a. A24001E_PURE_Rb_WB_041125
Whole cell lysates (15 µg total protein per lane) from HeLa S3 cells untreated (-) or treated with Nocodazole to arrest the cell cycle at G2/M stage (+) were resolved by SDS-PAGE using 4-12% Bis-Tris gels. Then transferred to a PVDF membrane and probed with Purified anti-Rb Phospho (Ser795) (clone A24001E). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. A24001E_PURE_Rb_WB_041125
    Whole cell lysates (15 µg total protein per lane) from HeLa S3 cells untreated (-) or treated with Nocodazole to arrest the cell cycle at G2/M stage (+) were resolved by SDS-PAGE using 4-12% Bis-Tris gels. Then transferred to a PVDF membrane and probed with Purified anti-Rb Phospho (Ser795) (clone A24001E). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. A24001E_PURE_Rb_IHC-P_041125
    IHC staining with Purified anti-Rb Phospho (Ser795) on formalin-fixed paraffin-embedded human ovarian cancer tissue. After antigen retrieval with 1X Citrate Buffer (Cat. No. 420902), the tissue was either untreated (left panels) or treated with Lambda Phosphatase Protein (LPP) (right panels). The tissue was then incubated with Purified anti-Rb Phospho (Ser795) (clone A24001E) and subsequently with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a 40X objective, showing the Alexa Fluor® 647 channel alone (bottom images) or merged with the DAPI channel (top images). Scale bar: 50 µm
  • C. A24001E_PURE_Rb_ICC_041125
    HeLa cells untreated (left panels) or treated with Nocodazole to arrest cell cycle at G2/M (right panels) were fixed and permeabilized with PFA and ice-cold methanol, and then intracellularly stained with Purified anti-Rb Phospho (Ser795) (clone A24001E) (bottom panels) or purified Mouse IgG1, κ Isotype Control (Cat. No. 400101) (top panels) followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Cells were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • d. A24001E_PURE_Rb_ICFC_041125
    HeLa cells treated with Nocodazole to arrest cell cycle at G2/M, were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-Rb Phospho (Ser795) (clone A24001E) (right panel) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 400102) (left panel), followed by Alexa Fluor® 647 Goat anti-mouse (Cat No. 405322). Cell cycle analysis was performed by DAPI staining (Cat. No. 422801).
Cat # Size Price Quantity Check Availability Save
640451 25 µg NOK1485
Check Availability


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640452 100 µg NOK3630
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Description

The Retinoblastoma protein (Rb) is a tumor suppressor that regulates cell cycle progression from G1 phase into the S phase. This regulation is crucial for preventing uncontrolled cell proliferation, which can lead to cancer. Hence, mutations or dysfunctions in the RB1 gene are associated with several cancers including retinoblastoma, lung, bladder, and breast cancers. Phosphorylation at serine 795 of the Rb protein plays a crucial role in regulating its function. The serine 795 phosphorylation leads to Rb dissociation from E2F transcription factor and allowing E2F to induce the transcription of cell cycle genes. This regulatory mechanism is vital for normal cell function and its dysregulation can contribute to cancer development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human Rb phosphorylated at serine 795
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

 

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). Similar results were also obtained using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803).

For use of this antibody in Immunocytochemistry (ICC), we recommend using PBS-Tween-20 0.05% as a wash buffer.  For use of this antibody in immunohistochemistry on formalin-fixed paraffinembedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

RRID
AB_3699068 (BioLegend Cat. No. 640451)
AB_3699068 (BioLegend Cat. No. 640452)

Antigen Details

Structure
The Rb protein is 928 amino acids in length and has a molecular weight of approximately 106 kDa.
Distribution

Nucleus, cytoplasmic during keratinocyte differentiation

Function
• Tumor Suppression: Rb acts as a tumor suppressor by inhibiting excessive cell growth and proliferation.
• Cell Cycle Regulation: Rb controls progression from the G1 phase to the S phase of the cell cycle by binding to and inhibiting E2F transcription factors.
• Transcription Regulation: Rb regulates the transcription of genes necessary for DNA synthesis and cell cycle progression.
• Apoptosis: Rb can also induce apoptosis under certain conditions, contributing to its role in preventing cancer.
Interaction
• E2F Transcription Factors: Rb binds to E2F transcription factors, inhibiting their activity and preventing the transcription of genes required for cell cycle progression.
• DP Proteins: Rb also interacts with DP proteins, which form heterodimers with E2F, enhancing the regulation of E2F activity.
• Cyclin-Dependent Kinases (CDKs): CDKs phosphorylate Rb, leading to its inactivation and allowing the cell cycle to progress.
• Histone Deacetylases (HDACs): Rb recruits HDACs to E2F target genes, leading to chromatin remodeling and transcriptional repression.
• Chromatin Remodeling Enzymes: Rb interacts with various chromatin remodeling enzymes, such as methylases and acetylases, to regulate gene expression.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins, Tumor Suppressors
Antigen References
  1. Rezaeian A, et al. 2023. Adv Protein Chem Struct Biol. 135:179.
  2. Janostiak R, et al. 2022. Cancers (Basel). 14:1265.
  3. Indovina P, et al. 2015. Oncotarget. 6:17873.
  4. Linn P, et al. 2021. Cancers (Basel). 13:3737.
Regulation
• Phosphorylation: Rb is regulated by phosphorylation, particularly by cyclin-dependent kinases (CDKs). Phosphorylation at specific sites, such as serine 795, inactivates Rb, allowing cell cycle progression.
• Dephosphorylation: Dephosphorylation of Rb reactivates its tumor suppressor functions, inhibiting cell cycle progression.
• Gene Mutations: Mutations in the RB1 gene can lead to loss of Rb function, contributing to the development of cancers such as retinoblastoma, lung, bladder, and breast cancers.
Gene ID
5925 View all products for this Gene ID
UniProt
View information about Rb Phospho Ser795 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.11.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
A24001E (See other available formats)
Regulatory Status
RUO
Other Names
RB1, Retinoblastoma-associated protein, p105-Rb, p110-RB1
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
a. A24001E_PURE_Rb_WB_041125
Whole cell lysates (15 µg total protein per lane) from HeLa S3 cells untreated (-) or treated with Nocodazole to arrest the cell cycle at G2/M stage (+) were resolved by SDS-PAGE using 4-12% Bis-Tris gels. Then transferred to a PVDF membrane and probed with Purified anti-Rb Phospho (Ser795) (clone A24001E). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. A24001E_PURE_Rb_WB_041125
    Whole cell lysates (15 µg total protein per lane) from HeLa S3 cells untreated (-) or treated with Nocodazole to arrest the cell cycle at G2/M stage (+) were resolved by SDS-PAGE using 4-12% Bis-Tris gels. Then transferred to a PVDF membrane and probed with Purified anti-Rb Phospho (Ser795) (clone A24001E). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. A24001E_PURE_Rb_IHC-P_041125
    IHC staining with Purified anti-Rb Phospho (Ser795) on formalin-fixed paraffin-embedded human ovarian cancer tissue. After antigen retrieval with 1X Citrate Buffer (Cat. No. 420902), the tissue was either untreated (left panels) or treated with Lambda Phosphatase Protein (LPP) (right panels). The tissue was then incubated with Purified anti-Rb Phospho (Ser795) (clone A24001E) and subsequently with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a 40X objective, showing the Alexa Fluor® 647 channel alone (bottom images) or merged with the DAPI channel (top images). Scale bar: 50 µm
  • C. A24001E_PURE_Rb_ICC_041125
    HeLa cells untreated (left panels) or treated with Nocodazole to arrest cell cycle at G2/M (right panels) were fixed and permeabilized with PFA and ice-cold methanol, and then intracellularly stained with Purified anti-Rb Phospho (Ser795) (clone A24001E) (bottom panels) or purified Mouse IgG1, κ Isotype Control (Cat. No. 400101) (top panels) followed by Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322). Cells were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • d. A24001E_PURE_Rb_ICFC_041125
    HeLa cells treated with Nocodazole to arrest cell cycle at G2/M, were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-Rb Phospho (Ser795) (clone A24001E) (right panel) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 400102) (left panel), followed by Alexa Fluor® 647 Goat anti-mouse (Cat No. 405322). Cell cycle analysis was performed by DAPI staining (Cat. No. 422801).
Cat # Size Price Quantity Check Availability Save
640451 25 µg NOK1485
Check Availability


Need larger quantities of this item?
Request Bulk Quote
640452 100 µg NOK3630
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The Retinoblastoma protein (Rb) is a tumor suppressor that regulates cell cycle progression from G1 phase into the S phase. This regulation is crucial for preventing uncontrolled cell proliferation, which can lead to cancer. Hence, mutations or dysfunctions in the RB1 gene are associated with several cancers including retinoblastoma, lung, bladder, and breast cancers. Phosphorylation at serine 795 of the Rb protein plays a crucial role in regulating its function. The serine 795 phosphorylation leads to Rb dissociation from E2F transcription factor and allowing E2F to induce the transcription of cell cycle genes. This regulatory mechanism is vital for normal cell function and its dysregulation can contribute to cancer development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human Rb phosphorylated at serine 795
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

 

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). Similar results were also obtained using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803).

For use of this antibody in Immunocytochemistry (ICC), we recommend using PBS-Tween-20 0.05% as a wash buffer.  For use of this antibody in immunohistochemistry on formalin-fixed paraffinembedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

RRID
AB_3699068 (BioLegend Cat. No. 640451)
AB_3699068 (BioLegend Cat. No. 640452)

Antigen Details

Structure
The Rb protein is 928 amino acids in length and has a molecular weight of approximately 106 kDa.
Distribution

Nucleus, cytoplasmic during keratinocyte differentiation

Function
• Tumor Suppression: Rb acts as a tumor suppressor by inhibiting excessive cell growth and proliferation.
• Cell Cycle Regulation: Rb controls progression from the G1 phase to the S phase of the cell cycle by binding to and inhibiting E2F transcription factors.
• Transcription Regulation: Rb regulates the transcription of genes necessary for DNA synthesis and cell cycle progression.
• Apoptosis: Rb can also induce apoptosis under certain conditions, contributing to its role in preventing cancer.
Interaction
• E2F Transcription Factors: Rb binds to E2F transcription factors, inhibiting their activity and preventing the transcription of genes required for cell cycle progression.
• DP Proteins: Rb also interacts with DP proteins, which form heterodimers with E2F, enhancing the regulation of E2F activity.
• Cyclin-Dependent Kinases (CDKs): CDKs phosphorylate Rb, leading to its inactivation and allowing the cell cycle to progress.
• Histone Deacetylases (HDACs): Rb recruits HDACs to E2F target genes, leading to chromatin remodeling and transcriptional repression.
• Chromatin Remodeling Enzymes: Rb interacts with various chromatin remodeling enzymes, such as methylases and acetylases, to regulate gene expression.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins, Tumor Suppressors
Antigen References
  1. Rezaeian A, et al. 2023. Adv Protein Chem Struct Biol. 135:179.
  2. Janostiak R, et al. 2022. Cancers (Basel). 14:1265.
  3. Indovina P, et al. 2015. Oncotarget. 6:17873.
  4. Linn P, et al. 2021. Cancers (Basel). 13:3737.
Regulation
• Phosphorylation: Rb is regulated by phosphorylation, particularly by cyclin-dependent kinases (CDKs). Phosphorylation at specific sites, such as serine 795, inactivates Rb, allowing cell cycle progression.
• Dephosphorylation: Dephosphorylation of Rb reactivates its tumor suppressor functions, inhibiting cell cycle progression.
• Gene Mutations: Mutations in the RB1 gene can lead to loss of Rb function, contributing to the development of cancers such as retinoblastoma, lung, bladder, and breast cancers.
Gene ID
5925 View all products for this Gene ID
UniProt
View information about Rb Phospho Ser795 on UniProt.org
Go To Top Version: 1    Revision Date: 04.11.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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