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TMB Substrate Set TMB Substrate Set

Pricing & Availability
Regulatory Status
RUO
Other Names
TMB Substrate
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
421101 110 mL NOK347
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Description

The TMB Substrate Set contains 110 ml TMB Substrate A and 110 ml TMB Substrate B, sufficient for twenty 96-well plates. It is formulated with 3, 3', 5, 5' tetramethyl benzidine (TMB). The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with BioLegend's Stop Solution (Cat. No. 423001), and read at 450 nm.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • 1 vial containing 110 mL of TMB Substrate A
  • 1 vial containing 110 mL of TMB Substrate B

Product Details

Storage & Handling
The TMB Substrate Set (TMB Substrate A and TMB Substrate B) should be stored between 2°C and 8°C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
Application

ELISA
Recommended Usage

TMB Substrate A should be clear and colorless.
TMB Substrate B should be colorless to a very light amber.
TMB Substrate is the mixture of equal volumes of TMB Substrate A with TMB Substrate B. For example, for one plate, mix 5.5 ml TMB Substrate A with 5.5 ml of TMB Substrate B in a clean container. Mix immediately before use.
After mixing the reagents together, TMB substrate working solution should be colorless or very faint blue.

Application Notes

Warm to room temperature prior to use. To prepare a working solution, mix equal volumes of TMB Substrate A and TMB Substrate B. Prepare only the amount needed for each assay run. After wells are completely washed, to remove unbound antibody and HRP, add 100 µl to 200 µl substrate solution to each well. Discard any remaining working solution after use. For endpoint assays, add 50 µl to 100 µl of Stop Solution (2N H2SO4 or 1M H3PO4), which changes the color from blue to yellow. Read at 450 nm (minus 570 nm for wavelength correction) within 30 minutes of stopping the reaction. For kinetic assays, read the blue color at 620 - 650 nm.

Application References
  1. Kim MS, et al. 2008. J Biochem. 143:497. PubMed
  2. McNally A, et al. 2011. PNAS. 108:7529. PubMed
  3. Santiago FW, et al. 2012. Vaccine. 30:4606. PubMed
  4. Wan LY, et al. 2014. Toxicol Sci. PubMed
  5. de Pablo P, et al. 2014. Ann Rheum Dis. 73:580. PubMed
Product Citations
  1. Kordyukova L, et al. 2023. Viruses. 15:. PubMed
  2. Yu M, et al. 2023. Nat Commun. 14:2164. PubMed
  3. Silva-Cayetano A, et al. 2023. Nat Immunol. . PubMed
  4. Lorenz P, et al. 2023. Front Immunol. 14:1192395. PubMed
  5. Nishimagi A, et al. 2023. Clin Exp Med. . PubMed
  6. Hellgren F, et al. 2023. Nat Commun. 14:3713. PubMed
  7. Sakamoto A, et al. 2022. Vaccines (Basel). 10: . PubMed
  8. Connelly GG, et al. 2022. Cell Rep Methods. 2:100273. PubMed
  9. Lu S, et al. 2023. Nat Commun. 14:17. PubMed
  10. Barajas-Mora EM, et al. 2023. Nat Immunol. 24:320. PubMed
  11. Gurram RK, et al. 2023. Cell Rep. 42:112073. PubMed
  12. Jameie F, et al. 2021. Iran J Parasitol. 1:16. PubMed
  13. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
  14. Bryant JD, et al. 2021. Brain Behav Immun. 97:204. PubMed
  15. Jameie F, et al. 2020. Arch Razi Inst. 75:327. PubMed
  16. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  17. Liu W, et al. 2016. J Virol. 90: 8496 - 8508. PubMed
  18. Hsiue EH, et al. 2021. Science. 371:. PubMed
  19. Peng L, et al. 2022. Cell Rep. 40:111160. PubMed
  20. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  21. Fertey J, et al. 2021. Methods Mol Biol. 2414:97. PubMed
  22. He Y, et al. 2021. Bio Protoc. 11:e3905. PubMed
  23. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  24. Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
  25. Alapati T, et al. 2021. Genes (Basel). 13:. PubMed
  26. Chang JH, et al. 2022. Vaccines (Basel). 10:. PubMed
  27. Sari AN, et al. 2022. Cancers (Basel). 14:. PubMed
  28. Stebegg M, et al. 2019. Nat Commun. 2.113194444. PubMed
  29. Sen K, et al. 2021. Front Immunol. 12:733539. PubMed
  30. Han JP, et al. 2022. Sci Adv. 8:eabj6901. PubMed
  31. Ding P, et al. 2022. Cell Rep. 39:110851. PubMed
  32. Walczewska M, et al. 2017. Adv Exp Med Biol. 10.1007/978-94-024-1079-2_41. PubMed
  33. Wu J, et al. 2020. Immunity. 53:115. PubMed
  34. Malik V, et al. 2020. Int J Mol Sci. 21:00. PubMed
  35. Ramos-Pinto L, et al. 2021. Animals (Basel). 11:. PubMed
  36. Nuvolone M, et al. 2017. PLoS One. 12:e0171923. PubMed
  37. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  38. Oh E, et al. 2018. Front Oncol. 0.716666667. PubMed
  39. Schwaderer J, et al. 2020. Cell Death Dis. 11:154. PubMed
  40. Debernardi S, et al. 2020. PLoS Med. 17:e1003489. PubMed
  41. Morgan S, et al. 2016. J Immunol. 196: 5014 - 5023. PubMed
  42. Younis RH, et al. 2021. Front Immunol. 596646:12. PubMed
  43. Fang Z, et al. 2022. Nat Commun. 13:3250. PubMed
  44. Groeneveld D, et al. 2020. J Hepatol. 72:146. PubMed
  45. Nogales A, et al. 2017. Virology. 500:1-10. PubMed
  46. Wan L, et al. 2014. Toxicol Sci. 139:83. PubMed
  47. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  48. Sun L, et al. 2021. Sci Rep. 11:12477. PubMed
  49. Wu J, et al. 2021. Nat Cell Biol. 23:75. PubMed
  50. Pagan JD, et al. 2018. Cell. 172:564. PubMed
  51. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  52. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
  53. Yang K, et al. 2020. Cell Chem Biol. 27:866. PubMed
  54. Falck-Jones S, et al. 2021. J Clin Invest. 131: . PubMed
  55. Vijver SV, et al. 2021. Front Immunol. 12:733561. PubMed
  56. Barbier M, et al. 2022. Front Immunol. 13:948431. PubMed
  57. Garg S, et al. 2019. Biotechniques. 67:166. PubMed
  58. Surawut S, et al. 2017. Sci Rep. 7:40006. PubMed
  59. McNally A, et al. 2011. Proc Natl Acad Sci U S A. 108:7529. PubMed
  60. Strazza M, et al. 2022. Mol Ther Methods Clin Dev. 27:380. PubMed
  61. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  62. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
  63. Kim M, et al. 2008. J Biochem. 143:497. PubMed
  64. Salehi S, et al. 2017. PLoS One. 10.1371/journal.pone.0163614. PubMed
  65. Miller H, et al. 2021. J Clin Microbiol. 59:e0096421. PubMed
  66. Leppkes M, et al. 2020. EBioMedicine. 58:102925. PubMed
  67. Parrish H, et al. 2016. Proc Natl Acad Sci U S A. 113: 3000 - 3005. PubMed
  68. Pablo P, et al. 2014. Ann Rheum Dis. 73:580. PubMed
  69. Guo Y, et al. 2021. Nat Commun. 12:2623. PubMed
  70. Reis B, et al. 2021. Mar Drugs. 19:. PubMed
  71. Knopf J, et al. 2022. Cells. 11:. PubMed
  72. Yang E, et al. 2015. Acta Biochim Biophys Sin. 47: 588-596. PubMed
  73. Santiago F, et al. 2012. Vaccine. 30:4606. PubMed
  74. Lee KS, et al. 2022. iScience. 25:105038. PubMed
  75. Yang K, et al. 2021. STAR Protoc. 2:100288. PubMed
  76. Kordyukova L, et al. 2023. Viruses. 15:. PubMed
  77. Yu M, et al. 2023. Nat Commun. 14:2164. PubMed
  78. Silva-Cayetano A, et al. 2023. Nat Immunol. . PubMed
  79. Lorenz P, et al. 2023. Front Immunol. 14:1192395. PubMed
  80. Nishimagi A, et al. 2023. Clin Exp Med. . PubMed
  81. Hellgren F, et al. 2023. Nat Commun. 14:3713. PubMed
  82. Sakamoto A, et al. 2022. Vaccines (Basel). 10: . PubMed
  83. Connelly GG, et al. 2022. Cell Rep Methods. 2:100273. PubMed
  84. Lu S, et al. 2023. Nat Commun. 14:17. PubMed
  85. Barajas-Mora EM, et al. 2023. Nat Immunol. 24:320. PubMed
  86. Gurram RK, et al. 2023. Cell Rep. 42:112073. PubMed
  87. Jameie F, et al. 2021. Iran J Parasitol. 1:16. PubMed
  88. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
  89. Bryant JD, et al. 2021. Brain Behav Immun. 97:204. PubMed
  90. Jameie F, et al. 2020. Arch Razi Inst. 75:327. PubMed
  91. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  92. Liu W, et al. 2016. J Virol. 90: 8496 - 8508. PubMed
  93. Hsiue EH, et al. 2021. Science. 371:. PubMed
  94. Peng L, et al. 2022. Cell Rep. 40:111160. PubMed
  95. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  96. Fertey J, et al. 2021. Methods Mol Biol. 2414:97. PubMed
  97. He Y, et al. 2021. Bio Protoc. 11:e3905. PubMed
  98. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  99. Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
  100. Alapati T, et al. 2021. Genes (Basel). 13:. PubMed
  101. Chang JH, et al. 2022. Vaccines (Basel). 10:. PubMed
  102. Sari AN, et al. 2022. Cancers (Basel). 14:. PubMed
  103. Stebegg M, et al. 2019. Nat Commun. 2.113194444. PubMed
  104. Sen K, et al. 2021. Front Immunol. 12:733539. PubMed
  105. Han JP, et al. 2022. Sci Adv. 8:eabj6901. PubMed
  106. Ding P, et al. 2022. Cell Rep. 39:110851. PubMed
  107. Walczewska M, et al. 2017. Adv Exp Med Biol. 10.1007/978-94-024-1079-2_41. PubMed
  108. Wu J, et al. 2020. Immunity. 53:115. PubMed
  109. Malik V, et al. 2020. Int J Mol Sci. 21:00. PubMed
  110. Ramos-Pinto L, et al. 2021. Animals (Basel). 11:. PubMed
  111. Nuvolone M, et al. 2017. PLoS One. 12:e0171923. PubMed
  112. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  113. Oh E, et al. 2018. Front Oncol. 0.716666667. PubMed
  114. Schwaderer J, et al. 2020. Cell Death Dis. 11:154. PubMed
  115. Debernardi S, et al. 2020. PLoS Med. 17:e1003489. PubMed
  116. Morgan S, et al. 2016. J Immunol. 196: 5014 - 5023. PubMed
  117. Younis RH, et al. 2021. Front Immunol. 596646:12. PubMed
  118. Fang Z, et al. 2022. Nat Commun. 13:3250. PubMed
  119. Groeneveld D, et al. 2020. J Hepatol. 72:146. PubMed
  120. Nogales A, et al. 2017. Virology. 500:1-10. PubMed
  121. Wan L, et al. 2014. Toxicol Sci. 139:83. PubMed
  122. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  123. Sun L, et al. 2021. Sci Rep. 11:12477. PubMed
  124. Wu J, et al. 2021. Nat Cell Biol. 23:75. PubMed
  125. Pagan JD, et al. 2018. Cell. 172:564. PubMed
  126. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  127. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
  128. Yang K, et al. 2020. Cell Chem Biol. 27:866. PubMed
  129. Falck-Jones S, et al. 2021. J Clin Invest. 131: . PubMed
  130. Vijver SV, et al. 2021. Front Immunol. 12:733561. PubMed
  131. Barbier M, et al. 2022. Front Immunol. 13:948431. PubMed
  132. Garg S, et al. 2019. Biotechniques. 67:166. PubMed
  133. Surawut S, et al. 2017. Sci Rep. 7:40006. PubMed
  134. McNally A, et al. 2011. Proc Natl Acad Sci U S A. 108:7529. PubMed
  135. Strazza M, et al. 2022. Mol Ther Methods Clin Dev. 27:380. PubMed
  136. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  137. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
  138. Kim M, et al. 2008. J Biochem. 143:497. PubMed
  139. Salehi S, et al. 2017. PLoS One. 10.1371/journal.pone.0163614. PubMed
  140. Miller H, et al. 2021. J Clin Microbiol. 59:e0096421. PubMed
  141. Leppkes M, et al. 2020. EBioMedicine. 58:102925. PubMed
  142. Parrish H, et al. 2016. Proc Natl Acad Sci U S A. 113: 3000 - 3005. PubMed
  143. Pablo P, et al. 2014. Ann Rheum Dis. 73:580. PubMed
  144. Guo Y, et al. 2021. Nat Commun. 12:2623. PubMed
  145. Reis B, et al. 2021. Mar Drugs. 19:. PubMed
  146. Knopf J, et al. 2022. Cells. 11:. PubMed
  147. Yang E, et al. 2015. Acta Biochim Biophys Sin. 47: 588-596. PubMed
  148. Santiago F, et al. 2012. Vaccine. 30:4606. PubMed
  149. Lee KS, et al. 2022. iScience. 25:105038. PubMed
  150. Yang K, et al. 2021. STAR Protoc. 2:100288. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 421101)

Antigen Details

Gene ID
NA

Related FAQs

What is the difference between your two substrate solutions: TMB Substrate Reagent Set (Cat. No. 421101) and TMB High Sensitivity Substrate Solution (Cat. No. 421501)?

TMB Substrate Reagent Set (Cat. No. 421101) contains two components, which need to be mixed immediately prior to use. The TMB High Sensitivity Substrate Solution (Cat. No. 421501) contains everything in one solution with special stabilizers. It is a high kinetic substrate solution which generally gives higher signal. The type of substrate used for an assay can affect the optical density (OD) achievable and the time required to achieve the desired OD.

Go To Top Version: 4    Revision Date: 05.30.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Pricing & Availability
Regulatory Status
RUO
Other Names
TMB Substrate
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
421101 110 mL NOK347
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The TMB Substrate Set contains 110 ml TMB Substrate A and 110 ml TMB Substrate B, sufficient for twenty 96-well plates. It is formulated with 3, 3', 5, 5' tetramethyl benzidine (TMB). The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with BioLegend's Stop Solution (Cat. No. 423001), and read at 450 nm.

Product Details
Technical Data Sheet (pdf)

Kit Details

Kit Contents
  • 1 vial containing 110 mL of TMB Substrate A
  • 1 vial containing 110 mL of TMB Substrate B

Product Details

Storage & Handling
The TMB Substrate Set (TMB Substrate A and TMB Substrate B) should be stored between 2°C and 8°C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
Application

ELISA
Recommended Usage

TMB Substrate A should be clear and colorless.
TMB Substrate B should be colorless to a very light amber.
TMB Substrate is the mixture of equal volumes of TMB Substrate A with TMB Substrate B. For example, for one plate, mix 5.5 ml TMB Substrate A with 5.5 ml of TMB Substrate B in a clean container. Mix immediately before use.
After mixing the reagents together, TMB substrate working solution should be colorless or very faint blue.

Application Notes

Warm to room temperature prior to use. To prepare a working solution, mix equal volumes of TMB Substrate A and TMB Substrate B. Prepare only the amount needed for each assay run. After wells are completely washed, to remove unbound antibody and HRP, add 100 µl to 200 µl substrate solution to each well. Discard any remaining working solution after use. For endpoint assays, add 50 µl to 100 µl of Stop Solution (2N H2SO4 or 1M H3PO4), which changes the color from blue to yellow. Read at 450 nm (minus 570 nm for wavelength correction) within 30 minutes of stopping the reaction. For kinetic assays, read the blue color at 620 - 650 nm.

Application References
  1. Kim MS, et al. 2008. J Biochem. 143:497. PubMed
  2. McNally A, et al. 2011. PNAS. 108:7529. PubMed
  3. Santiago FW, et al. 2012. Vaccine. 30:4606. PubMed
  4. Wan LY, et al. 2014. Toxicol Sci. PubMed
  5. de Pablo P, et al. 2014. Ann Rheum Dis. 73:580. PubMed
Product Citations
  1. Kordyukova L, et al. 2023. Viruses. 15:. PubMed
  2. Yu M, et al. 2023. Nat Commun. 14:2164. PubMed
  3. Silva-Cayetano A, et al. 2023. Nat Immunol. . PubMed
  4. Lorenz P, et al. 2023. Front Immunol. 14:1192395. PubMed
  5. Nishimagi A, et al. 2023. Clin Exp Med. . PubMed
  6. Hellgren F, et al. 2023. Nat Commun. 14:3713. PubMed
  7. Sakamoto A, et al. 2022. Vaccines (Basel). 10: . PubMed
  8. Connelly GG, et al. 2022. Cell Rep Methods. 2:100273. PubMed
  9. Lu S, et al. 2023. Nat Commun. 14:17. PubMed
  10. Barajas-Mora EM, et al. 2023. Nat Immunol. 24:320. PubMed
  11. Gurram RK, et al. 2023. Cell Rep. 42:112073. PubMed
  12. Jameie F, et al. 2021. Iran J Parasitol. 1:16. PubMed
  13. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
  14. Bryant JD, et al. 2021. Brain Behav Immun. 97:204. PubMed
  15. Jameie F, et al. 2020. Arch Razi Inst. 75:327. PubMed
  16. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  17. Liu W, et al. 2016. J Virol. 90: 8496 - 8508. PubMed
  18. Hsiue EH, et al. 2021. Science. 371:. PubMed
  19. Peng L, et al. 2022. Cell Rep. 40:111160. PubMed
  20. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  21. Fertey J, et al. 2021. Methods Mol Biol. 2414:97. PubMed
  22. He Y, et al. 2021. Bio Protoc. 11:e3905. PubMed
  23. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  24. Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
  25. Alapati T, et al. 2021. Genes (Basel). 13:. PubMed
  26. Chang JH, et al. 2022. Vaccines (Basel). 10:. PubMed
  27. Sari AN, et al. 2022. Cancers (Basel). 14:. PubMed
  28. Stebegg M, et al. 2019. Nat Commun. 2.113194444. PubMed
  29. Sen K, et al. 2021. Front Immunol. 12:733539. PubMed
  30. Han JP, et al. 2022. Sci Adv. 8:eabj6901. PubMed
  31. Ding P, et al. 2022. Cell Rep. 39:110851. PubMed
  32. Walczewska M, et al. 2017. Adv Exp Med Biol. 10.1007/978-94-024-1079-2_41. PubMed
  33. Wu J, et al. 2020. Immunity. 53:115. PubMed
  34. Malik V, et al. 2020. Int J Mol Sci. 21:00. PubMed
  35. Ramos-Pinto L, et al. 2021. Animals (Basel). 11:. PubMed
  36. Nuvolone M, et al. 2017. PLoS One. 12:e0171923. PubMed
  37. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  38. Oh E, et al. 2018. Front Oncol. 0.716666667. PubMed
  39. Schwaderer J, et al. 2020. Cell Death Dis. 11:154. PubMed
  40. Debernardi S, et al. 2020. PLoS Med. 17:e1003489. PubMed
  41. Morgan S, et al. 2016. J Immunol. 196: 5014 - 5023. PubMed
  42. Younis RH, et al. 2021. Front Immunol. 596646:12. PubMed
  43. Fang Z, et al. 2022. Nat Commun. 13:3250. PubMed
  44. Groeneveld D, et al. 2020. J Hepatol. 72:146. PubMed
  45. Nogales A, et al. 2017. Virology. 500:1-10. PubMed
  46. Wan L, et al. 2014. Toxicol Sci. 139:83. PubMed
  47. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  48. Sun L, et al. 2021. Sci Rep. 11:12477. PubMed
  49. Wu J, et al. 2021. Nat Cell Biol. 23:75. PubMed
  50. Pagan JD, et al. 2018. Cell. 172:564. PubMed
  51. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  52. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
  53. Yang K, et al. 2020. Cell Chem Biol. 27:866. PubMed
  54. Falck-Jones S, et al. 2021. J Clin Invest. 131: . PubMed
  55. Vijver SV, et al. 2021. Front Immunol. 12:733561. PubMed
  56. Barbier M, et al. 2022. Front Immunol. 13:948431. PubMed
  57. Garg S, et al. 2019. Biotechniques. 67:166. PubMed
  58. Surawut S, et al. 2017. Sci Rep. 7:40006. PubMed
  59. McNally A, et al. 2011. Proc Natl Acad Sci U S A. 108:7529. PubMed
  60. Strazza M, et al. 2022. Mol Ther Methods Clin Dev. 27:380. PubMed
  61. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  62. Phan TS, et al. 2021. Sci Adv. 7:. PubMed
  63. Kim M, et al. 2008. J Biochem. 143:497. PubMed
  64. Salehi S, et al. 2017. PLoS One. 10.1371/journal.pone.0163614. PubMed
  65. Miller H, et al. 2021. J Clin Microbiol. 59:e0096421. PubMed
  66. Leppkes M, et al. 2020. EBioMedicine. 58:102925. PubMed
  67. Parrish H, et al. 2016. Proc Natl Acad Sci U S A. 113: 3000 - 3005. PubMed
  68. Pablo P, et al. 2014. Ann Rheum Dis. 73:580. PubMed
  69. Guo Y, et al. 2021. Nat Commun. 12:2623. PubMed
  70. Reis B, et al. 2021. Mar Drugs. 19:. PubMed
  71. Knopf J, et al. 2022. Cells. 11:. PubMed
  72. Yang E, et al. 2015. Acta Biochim Biophys Sin. 47: 588-596. PubMed
  73. Santiago F, et al. 2012. Vaccine. 30:4606. PubMed
  74. Lee KS, et al. 2022. iScience. 25:105038. PubMed
  75. Yang K, et al. 2021. STAR Protoc. 2:100288. PubMed
  76. Kordyukova L, et al. 2023. Viruses. 15:. PubMed
  77. Yu M, et al. 2023. Nat Commun. 14:2164. PubMed
  78. Silva-Cayetano A, et al. 2023. Nat Immunol. . PubMed
  79. Lorenz P, et al. 2023. Front Immunol. 14:1192395. PubMed
  80. Nishimagi A, et al. 2023. Clin Exp Med. . PubMed
  81. Hellgren F, et al. 2023. Nat Commun. 14:3713. PubMed
  82. Sakamoto A, et al. 2022. Vaccines (Basel). 10: . PubMed
  83. Connelly GG, et al. 2022. Cell Rep Methods. 2:100273. PubMed
  84. Lu S, et al. 2023. Nat Commun. 14:17. PubMed
  85. Barajas-Mora EM, et al. 2023. Nat Immunol. 24:320. PubMed
  86. Gurram RK, et al. 2023. Cell Rep. 42:112073. PubMed
  87. Jameie F, et al. 2021. Iran J Parasitol. 1:16. PubMed
  88. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
  89. Bryant JD, et al. 2021. Brain Behav Immun. 97:204. PubMed
  90. Jameie F, et al. 2020. Arch Razi Inst. 75:327. PubMed
  91. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  92. Liu W, et al. 2016. J Virol. 90: 8496 - 8508. PubMed
  93. Hsiue EH, et al. 2021. Science. 371:. PubMed
  94. Peng L, et al. 2022. Cell Rep. 40:111160. PubMed
  95. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  96. Fertey J, et al. 2021. Methods Mol Biol. 2414:97. PubMed
  97. He Y, et al. 2021. Bio Protoc. 11:e3905. PubMed
  98. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  99. Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
  100. Alapati T, et al. 2021. Genes (Basel). 13:. PubMed
  101. Chang JH, et al. 2022. Vaccines (Basel). 10:. PubMed
  102. Sari AN, et al. 2022. Cancers (Basel). 14:. PubMed
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RRID
SCR_001134 (BioLegend Cat. No. 421101)

Antigen Details

Gene ID
NA

Related FAQs

What is the difference between your two substrate solutions: TMB Substrate Reagent Set (Cat. No. 421101) and TMB High Sensitivity Substrate Solution (Cat. No. 421501)?

TMB Substrate Reagent Set (Cat. No. 421101) contains two components, which need to be mixed immediately prior to use. The TMB High Sensitivity Substrate Solution (Cat. No. 421501) contains everything in one solution with special stabilizers. It is a high kinetic substrate solution which generally gives higher signal. The type of substrate used for an assay can affect the optical density (OD) achievable and the time required to achieve the desired OD.

Go To Top Version: 4    Revision Date: 05.30.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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