Product Highlight

Our newly launched ELISpot MAX™Deluxe sets are optimized and designed for exceptional sensitivity in detecting secreted cytokines at the single-cell level. Identify and quantify antigen-specific effector cells secreting human IFN-γ, IL-10, TNF-α, and IL-6 to advance your immune monitoring research.

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What are ELISpot assays?

The Enzyme-Linked Immunospot (ELISpot) assay is a powerful immunological technique designed to detect and quantify cytokine-secreting or analyte-releasing cells at the single-cell level. It is based on the principles of the sandwich ELISA method, but extends its capabilities to identify individual analyte secreting cells among large populations. ELISpot is an invaluable tool for studying antigen-specific immune responses in areas such as vaccine development, infectious diseases, and cancer immunotherapy.

Benefits of ELISpot MAX™ Deluxe Kits 

• High Sensitivity: Detects individual analyte secreting cells as low as 1 in 100,000 cells

• Flexibility: User-defined antigen selection allows flexibility in studying a variety of antigen-specific immune responses across multiple research areas

• Ease of Use: Straightforward protocol that is easy to learn and implement in most laboratory settings

How ELISpot Works

The assay leverages plate-bound antibodies to capture analytes secreted by cells directly in their immediate environment. These captured analytes form complexes that are visualized using an enzymatic reaction, resulting in a localized, blue colored spot. Each visible spot corresponds to a single cell that secreted the analyte, hence the name "ELISpot."

Principle of ELISpot assay

ELISpot MAX Deluxe Set Human IFN-γ 

Human peripheral blood mononuclear cells (PBMC) were incubated for 20 hours without stimuli or with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301), Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303), or Ultra-LEAF™ Purified anti-human CD3 antibody (Cat. No. 300331). 

Features of our ELISpot sets