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Biotin anti-human Angiopoietin-2 Antibody

Pricing & Availability
Clone
A15124B (See other available formats)
Regulatory Status
RUO
Other Names
Angiopoietin 2, ANG2, Ang-2, ANGPT2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15124B_Biotin_Angiopoietin-2_Antibody_022024
Direct ELISA showing biotin anti-human Angiopoietin-2 (clone A15124B) bound to plate-immobilized recombinant human Angiopoietin-2 (Cat. Nos. 753102, 753104, and 753106). The wells were incubated with serially diluted primary biotin antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A15124B_Biotin_Angiopoietin-2_Antibody_022024
    Direct ELISA showing biotin anti-human Angiopoietin-2 (clone A15124B) bound to plate-immobilized recombinant human Angiopoietin-2 (Cat. Nos. 753102, 753104, and 753106). The wells were incubated with serially diluted primary biotin antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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629453 50 µg 396€
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Description

Angiopoietin-2, also known as ANGPT2, is a member of angiopoietins, which plays essential roles in embryonic and postnatal angiogenesis. The structure of the mature form of ANGPT2 contains an N-terminal coiled-coil domain, which mediates multimerization of the protein, along with a C-terminal fibrinogen-like domain, which mediates the receptor binding. Both ANGPT1 and ANGPT2 are ligands for the receptor tyrosine kinase, Tie-2. ANGPT2 appears to exist predominantly as a homodimer, but it is also capable of forming multimers while ANGPT1 predominantly exists as multimers. It has been shown that ANGPT2 is able to promote vascular leakage and neutrophil migration. ANGPT2 can also sensitize TNF-α-induced monocyte adhesion. Thus, it is considered a proinflammatory factor. Although ANGPT2 is not required for arterial vascular development, its expression in the tissue suggests that it may play a role in the maintenance of these vessels. This conclusion is also supported by the observation that high concentrations of ANGPT2 activate the same survival signaling pathway as ANGPT1 to prevent endothelial cell apoptosis via Tie2/Akt. Additionally, ANGPT2 was demonstrated to function as an agonist in the absence of ANGPT1, but in its presence it acts as an antagonist. Human ANGPT2 shares 86% amino acid sequence homology with its murine counterpart.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant Angiopoietin-2 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A15124B) could function as a detection antibody when paired with purified anti-human Angiopoietin-2 (Cat No. TBD) (clone W19419E) as the capture antibody and using Recombinant Human Angiopoietin-2 (carrier-free) (Cat. Nos. 753102, 753104, and 753106) protein as the assay standard.

Antigen Details

Structure
Oligomer
Distribution

Placenta, uterus, ovaries, and vascular endothelial cells

Function
ANGPT2 plays a role in angiogenesis and monocyte adhesion. Hyperoxia induces ANGPT2 expression in the lung epithelial cells.
Interaction
Monocytes and endothelial cells
Ligand/Receptor
Tie-2
Cell Type
Embryonic Stem Cells
Biology Area
Angiogenesis, Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Augustin HG, et al. 2009. Nat Rev Mol. Biol. 10:165-77.
  2. Ward NL and Dumont DJ. 2002. Semin Cell Dev Biol. 1:19-27.
  3. Roviezzo F, et al. 2005. J Pharmacol Exp Ther. 314:738-44.
  4. Sturn DH, et al. 2005. Microcirculation. 12:393-403.
  5. Murdoch C, et al. 2007. J Immunol. 178:7405-11.
  6. Yuan HT, et al. 2009. Mol Cell Biol. 29:2011-22.
  7. Procopio WN, et al. 1999. J Biol Chem. 274:30196-201.
  8. Bhandari V, et al. 2006. Nature Med. 12:1286-93.
Gene ID
285 View all products for this Gene ID
UniProt
View information about Angiopoietin-2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 02-21-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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