Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Denmark (DKK) DKK
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Norway (NOK) NOK
Sweden (SEK) SEK
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Regulatory Status
- RUO
- Other Names
- PD-L1 (CD274), OX-2 (CD200), PD-L2 (CD273), CTLA-4 (CD152), B7-2 (CD86), TIGIT (Vstm3), PD-1 (CD279), PVR (CD155), HVEM (CD270), IL-2Rα (sCD25)
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 741599 | 100 tests | 1128€ | ||||
Immune checkpoint molecules are key regulators of immune activation, playing a critical role in maintaining immune homeostasis through a complex interplay of co-stimulatory and inhibitory signals. While these checkpoints help prevent autoimmunity in healthy individuals, cancer cells can exploit them by altering their expression to suppress anti-tumor immune responses during host immuno-editing. Accurate quantification of checkpoint molecule expression is therefore essential to ongoing biomedical research, supporting the development of therapeutic strategies to overcome tumor-mediated immune evasion and guiding the design of next-generation immunotherapies.
The LEGENDplex™ Mouse Immune Checkpoint Panel 1 (10-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 10 mouse immune checkpoint proteins including PD-L1 (CD274), OX-2 (CD200), PD-L2 (CD273), CTLA-4 (CD152), B7-2 (CD86), TIGIT (Vstm3), PD-1 (CD279), IL-2Rα (sCD25), PVR (CD155) and HVEM (CD270). This panel provides higher sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on serum, plasma and cell culture supernatant sample. The LEGENDplex™ Mouse Immune Checkpoint Panel 1 (10-plex) is designed to allow flexible customization within the panel. It can also be divided into subpanels.
Kit Details
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- LEGENDplex™ Mouse Immune Checkpoint Panel 1 Capture Beads
- LEGENDplex™ Mouse Immune Checkpoint Panel 1 Detection Abs
- LEGENDplex™ Mouse Immune Checkpoint Panel 1 Standard
- SA-PE
- LEGENDplex™ Assay Buffer
- LEGENDplex™ Wash Buffer, 20X
- V-bottom Plate
- Plate Sealers
- Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- Centrifuge with a swinging bucket adaptor for microtiter plates (if using V-bottom plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
- Manual
Product Details
- Verified Reactivity
- Mouse
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here.
Antigen Details
- Cell Type
- Antigen-presenting cells, T cells
- Biology Area
- Adaptive Immunity, Costimulatory Molecules, Immunology, Inhibitory Molecules, Innate Immunity, Synaptic Biology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Gene ID
- 60533 View all products for this Gene ID 4345 View all products for this Gene ID 58205 View all products for this Gene ID 12477 View all products for this Gene ID 16182 View all products for this Gene ID 100043314 View all products for this Gene ID 18566 View all products for this Gene ID 52118 View all products for this Gene ID 230979 View all products for this Gene ID 3559 View all products for this Gene ID
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
-
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
-
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
-
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
Follow Us