Spark Blue™ 574 anti-human CD39 (Flexi-Fluor™) Antibody, CD39, A1

Pricing & Availability

Clone: A1 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: gp80, E-ATPDase, NTPDase-1, ecto-apyrase, Ec3.6.1.5
Isotype: Mouse IgG1, κ
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Product Citations: publications

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285181

50 µg

316€

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Description

Human CD39 is an integral membrane protein with two transmembrane domains. It exists as a homotetramer. Expression of CD39 is found on activated lymphocytes, a subset of T cells and B cells, and dendritic cells with weak staining on monocytes and granulocytes. CD39 and CD73 have been found on regulatory T cells, specifically the effector/memory like T cells. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. CD39 is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PHA activated human lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The A1 antibody binds to the human CD39 cell surface antigen and has been shown to block MHC independent target cell recognition by hapten-specific CTL. Additional reported applications (for the relevant formats) include: in vitro CD39 blockade³, immunofluorescence⁴, immunohistochemistry⁶, and spatial biology (IBEX)^7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking assays (contact our custom solutions team).
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3662521 (BioLegend Cat. No. 285181)

Antigen Details

Distribution: Activated lymphocytes, and also on a subset of T cells, regulatory T cells, B cells, and dendritic cells.
Cell Type: B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Gene ID: NA
UniProt: View information about CD39 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

What if my requested antibody-dye combination doesn't perform as expected?

Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.

Other Formats

View All CD39 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD39 (Maxpar® Ready)	A1	FC,CyTOF®,IHC-P
PE/Dazzle™ 594 anti-human CD39	A1	FC
APC/Cyanine7 anti-human CD39	A1	FC
Brilliant Violet 711™ anti-human CD39	A1	FC
APC/Fire™ 750 anti-human CD39	A1	FC
Alexa Fluor® 594 anti-human CD39	A1	IHC-P
TotalSeq™-A0176 anti-human CD39	A1	PG
Brilliant Violet 605™ anti-human CD39	A1	FC
TotalSeq™-C0176 anti-human CD39	A1	PG
Brilliant Violet 785™ anti-human CD39	A1	FC
TotalSeq™-B0176 anti-human CD39	A1	PG
Brilliant Violet 510™ anti-human CD39	A1	FC
Brilliant Violet 421™ anti-human CD39	A1	FC,IHC-P
Purified anti-human CD39	A1	FC,IHC-P,Block
Biotin anti-human CD39	A1	FC
FITC anti-human CD39	A1	FC
PE anti-human CD39	A1	FC,SB
APC anti-human CD39	A1	FC
PE/Cyanine7 anti-human CD39	A1	FC
PerCP/Cyanine5.5 anti-human CD39	A1	FC
TotalSeq™-D0176 anti-human CD39	A1	PG
PE/Fire™ 810 anti-human CD39 Antibody	A1	FC
PE/Cyanine5 anti-human CD39	A1	FC
PerCP/Fire™ 806 anti-human CD39	A1	FC
Spark NIR™ 685 anti-human CD39	A1	FC
Spark Red™ 718 anti-human CD39 (Flexi-Fluor™)	A1	FC
Alexa Fluor® 700 anti-human CD39	A1	FC
PE/Fire™ 744 anti-human CD39	A1	FC
Spark Blue™ 574 anti-human CD39 (Flexi-Fluor™)	A1	FC
Spark Blue™ 550 anti-human CD39 (Flexi-Fluor™)	A1	FC
Spark PLUS UV395™ anti-human CD39	A1	FC
Alexa Fluor® 647 anti-human CD39	A1	FC,IHC-P
Spark YG™ 581 anti-human CD39 (Flexi-Fluor™)	A1	FC
Spark YG™ 593 anti-human CD39 (Flexi-Fluor™)	A1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09-06-2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.