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Annexin V Binding Buffer Annexin V Binding Buffer

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Other Names
Annexin-V-Binding Buffer
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422201 100 mL SEK303
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Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. 
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  3. Turner JE, et al. 2013. J Exp Med. PubMed
  4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
  8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
  9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
  11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed
Product Citations
  1. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  2. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  3. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  4. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  5. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  6. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  7. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  8. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  9. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  10. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  11. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  14. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  15. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  16. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  17. Vajda F, et al. 2022. Cells. 11:. PubMed
  18. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  19. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  20. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  21. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  22. Quintela M, et al. 2023. Br J Cancer. . PubMed
  23. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  24. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  25. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  26. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  27. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  28. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  29. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  30. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  31. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  32. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  33. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  34. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  35. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  36. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  37. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  38. McNeal AS, et al. 2021. Elife. 10:. PubMed
  39. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  40. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  41. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  42. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  43. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  44. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  45. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  46. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  47. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  48. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  49. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  50. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  51. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  52. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  53. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  54. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  55. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  56. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  57. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  58. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  59. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  60. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  61. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  62. Wu J, et al. 2020. Immunity. 53:115. PubMed
  63. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  64. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  65. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  66. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  67. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  68. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  69. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  70. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  71. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  72. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  73. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  74. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  75. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  76. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  77. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  78. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  79. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  80. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  81. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  82. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  83. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  84. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  85. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  86. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  87. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  88. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  89. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  90. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  91. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  92. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  93. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  94. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  95. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  96. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  97. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  98. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  99. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  100. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  101. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  102. Harro CM, et al. 2020. J Clin Invest. . PubMed
  103. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  104. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  105. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  106. Dong G, et al. 2020. Haematologica. . PubMed
  107. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  108. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  109. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  110. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  111. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  112. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  113. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  114. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  115. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  116. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  117. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  118. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  119. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  120. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  121. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  122. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  123. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  124. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  125. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  126. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  127. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  128. Jatho A, et al. 2022. Cells. 11:. PubMed
  129. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  130. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  131. Vulin M, et al. 2022. Oncogene. :. PubMed
  132. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  133. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  134. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  135. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  136. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  137. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  138. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  139. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  140. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  141. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  142. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  143. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  144. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  145. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  146. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  147. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  148. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  149. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  150. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  151. Vajda F, et al. 2022. Cells. 11:. PubMed
  152. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  153. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  154. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  155. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  156. Quintela M, et al. 2023. Br J Cancer. . PubMed
  157. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  158. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  159. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  160. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  161. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  162. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  163. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  164. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  165. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  166. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  167. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  168. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  169. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  170. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  171. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  172. McNeal AS, et al. 2021. Elife. 10:. PubMed
  173. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  174. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  175. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  176. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  177. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  178. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  179. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  180. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  181. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  182. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  184. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  185. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  186. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  187. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  188. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  189. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  190. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  191. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  192. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  193. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  194. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  195. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  196. Wu J, et al. 2020. Immunity. 53:115. PubMed
  197. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  198. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  199. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  200. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  201. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  202. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  203. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  204. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  205. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  206. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  207. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  208. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  209. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  210. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  211. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  212. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  213. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  214. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  215. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  216. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  217. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  218. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  219. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  220. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  221. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  222. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  223. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  224. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  225. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  226. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  227. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  228. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  229. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  230. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  231. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  232. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  233. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  234. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  235. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  236. Harro CM, et al. 2020. J Clin Invest. . PubMed
  237. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  238. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  239. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  240. Dong G, et al. 2020. Haematologica. . PubMed
  241. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  242. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  243. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  244. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  245. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  246. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  247. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  248. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  249. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  250. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  251. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  252. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  253. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  254. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  255. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  256. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  257. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  258. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  259. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  260. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  261. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  262. Jatho A, et al. 2022. Cells. 11:. PubMed
  263. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  264. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  265. Vulin M, et al. 2022. Oncogene. :. PubMed
  266. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  267. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  268. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed

Antigen Details

Gene ID
NA

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 5    Revision Date: 03-15-2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Pricing & Availability
Regulatory Status
RUO
Other Names
Annexin-V-Binding Buffer
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422201 100 mL SEK303
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Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining

  1. Wash cells twice with cold BioLegend Cell Staining Buffer (Cat. No. 420201) and then resuspend cells in Annexin V Binding Buffer (Cat. No. 422201) at a concentration of 1x106 cells/mL.
  2. Transfer 100 µL of cell suspension in 5 mL test tube.
  3. Add 5 µL of fluorochrome conjugated Annexin V.
  4. Stain with a viability dye, such as PI (Cat. No. 421301), 7-AAD (Cat. Nos. 420403 & 420404), or Helix NP dyes (Cat. Nos. 425301, 425303, & 425305), if desired. 
  5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
  6. Add 400* µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. *For more concentrated samples, add a minimum of 200 µl of Annexin V Binding Buffer in this step.
  7. Analyze by flow cytometry.

For a better experience detecting apoptosis, we now recommend Apotracker™. Cell staining with Apotracker™ is Calcium independent. Thus, no special buffers are required, and the protocol can be shortened for single-step co-staining with other reagents.

Application References
  1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
  2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  3. Turner JE, et al. 2013. J Exp Med. PubMed
  4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
  8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
  9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
  11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed
Product Citations
  1. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  2. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  3. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  4. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  5. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  6. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  7. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  8. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  9. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  10. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  11. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  12. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  13. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  14. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  15. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  16. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  17. Vajda F, et al. 2022. Cells. 11:. PubMed
  18. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  19. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  20. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  21. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  22. Quintela M, et al. 2023. Br J Cancer. . PubMed
  23. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  24. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  25. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  26. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  27. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  28. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  29. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  30. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  31. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  32. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  33. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  34. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  35. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  36. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  37. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  38. McNeal AS, et al. 2021. Elife. 10:. PubMed
  39. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  40. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  41. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  42. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  43. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  44. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  45. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  46. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  47. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  48. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  49. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  50. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  51. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  52. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  53. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  54. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  55. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  56. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  57. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  58. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  59. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  60. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  61. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  62. Wu J, et al. 2020. Immunity. 53:115. PubMed
  63. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  64. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  65. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  66. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  67. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  68. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  69. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  70. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  71. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  72. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  73. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  74. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  75. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  76. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  77. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  78. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  79. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  80. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  81. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  82. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  83. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  84. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  85. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  86. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  87. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  88. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  89. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  90. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  91. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  92. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  93. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  94. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  95. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  96. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  97. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  98. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  99. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  100. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  101. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  102. Harro CM, et al. 2020. J Clin Invest. . PubMed
  103. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  104. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  105. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  106. Dong G, et al. 2020. Haematologica. . PubMed
  107. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  108. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  109. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  110. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  111. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  112. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  113. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  114. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  115. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  116. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  117. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  118. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  119. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  120. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  121. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  122. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  123. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  124. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  125. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  126. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  127. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  128. Jatho A, et al. 2022. Cells. 11:. PubMed
  129. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  130. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  131. Vulin M, et al. 2022. Oncogene. :. PubMed
  132. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  133. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  134. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed
  135. Shrestha C,et al. 2017. PLoS One. 10.1371/journal.pone.0186169. PubMed
  136. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  137. Liu M, et al. 2021. Mol Med Rep. 23:00. PubMed
  138. Ocklenburg T, et al. 2021. Sci Rep. 7199:11. PubMed
  139. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  140. Forika G, et al. 2021. Pathol Oncol Res. 27:1610048. PubMed
  141. Tsuji M 2014. Mol Biol Cell. 25:2116. PubMed
  142. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  143. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  144. Clusmann J, et al. 2022. Cell Death Dis. 13:702. PubMed
  145. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  146. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  147. Cappellesso F, et al. 2022. Nat Cancer. 3:1464. PubMed
  148. Muckenhuber M, et al. 2023. Am J Transplant. 23:84. PubMed
  149. Zhao Q, et al. 2023. Nat Commun. 14:735. PubMed
  150. Wang YH, et al. 2022. Sci Adv. 8:eabn6552. PubMed
  151. Vajda F, et al. 2022. Cells. 11:. PubMed
  152. Chu CY, et al. 2023. Sci Rep. 13:3943. PubMed
  153. Riera-Domingo C, et al. 2023. Sci Adv. 9:eadd5028. PubMed
  154. Bujarrabal-Dueso A, et al. 2023. Nat Struct Mol Biol. 30:475. PubMed
  155. Quintela M, et al. 2023. Clin Epigenetics. 15:63. PubMed
  156. Quintela M, et al. 2023. Br J Cancer. . PubMed
  157. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  158. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  159. Svoronos AA, et al. 2021. iScience. 24:103208. PubMed
  160. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  161. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  162. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  163. Itkin T, et al. 2016. Nature. 532: 323-328. PubMed
  164. Hergott C, et al. 2016. Blood. 127: 2460 - 2471. PubMed
  165. Olelewe C, et al. 2022. iScience. 25:104340. PubMed
  166. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  167. Venter C, et al. 2017. Cell Physiol Biochem. 43:1288. PubMed
  168. Terashima S, et al. 2017. J Radiat Res. 10.1093/jrr/rrx025. PubMed
  169. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  170. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  171. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  172. McNeal AS, et al. 2021. Elife. 10:. PubMed
  173. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  174. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  175. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  176. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  177. Dregalla RC, et al. 2021. Stem Cell Res Ther. 12:547. PubMed
  178. Sun V, et al. 2021. Front Immunol. 12:716661. PubMed
  179. Herman KD, et al. 2022. Front Immunol. 13:956991. PubMed
  180. Soprano M, et al. 2016. PLoS One. 11: 0155970. PubMed
  181. Li L, et al. 2016. Cell Death Dis. 7:e2439. PubMed
  182. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Naaman H,et al. 2017. PLoS One. 10.1371/journal.pone.0187077. PubMed
  184. Tian AL, et al. 2021. Cell Death Dis. 12:6. PubMed
  185. Elakad O, et al. 2022. NPJ Precis Oncol. 6:52. PubMed
  186. Deng J, et al. 2021. Cell Death Dis. 12:978. PubMed
  187. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  188. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  189. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  190. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  191. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  192. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  193. Hickman O, et al. 2016. Br J Cancer. 10.1038/bjc.2016.91. PubMed
  194. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  195. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  196. Wu J, et al. 2020. Immunity. 53:115. PubMed
  197. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  198. Luca E, et al. 2020. Mol Metab. 36:100967. PubMed
  199. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  200. Li P, et al. 2016. Mol Biol Cell. 27: 2542 - 2553. PubMed
  201. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  202. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  203. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  204. Xie S, et al. 2021. Sci Rep. 0.660416667. PubMed
  205. Wang P, et al. 2019. Nat Commun. 0.940972222. PubMed
  206. Celus W, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  207. Burbulla L, et al. 2014. Cell Death Dis. 5:1180. PubMed
  208. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  209. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  210. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  211. Cheng CC, et al. 2020. Elife. 9:00. PubMed
  212. Leiendecker L, et al. 2020. EMBO Mol Med. 12:e12525. PubMed
  213. Carosi JM, et al. 2021. Autophagy. 17:2217. PubMed
  214. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  215. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  216. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  217. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  218. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  219. Thakran S, et al. 2015. Invest Ophthalmol Vis Sci . 56:177. PubMed
  220. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  221. Deventhiran J, et al. 2016. J Virol. 90: 222 - 231. PubMed
  222. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  223. Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
  224. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  225. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  226. Becker A, et al. 2016. Sci Rep. 6: 33847. PubMed
  227. Hopkins T, et al. 2015. Nucleic Acids Res. 10.1093/nar/gkv1515. PubMed
  228. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  229. Wang M, et al. 2018. Diabetologia. 62:335. PubMed
  230. Textor A, et al. 2021. Cancers (Basel). 13:. PubMed
  231. Molinar-Inglis O, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  232. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  233. Erdogan M, et al. 2022. Chem Biodivers. 19:e202200659. PubMed
  234. Zhang S, et al. 2018. Sci Rep. 8:17066. PubMed
  235. Lee HW, et al. 2018. Am J Physiol Renal Physiol. 314:F280. PubMed
  236. Harro CM, et al. 2020. J Clin Invest. . PubMed
  237. Hasselluhn MC, et al. 2019. Cell Death Dis. 0.726388889. PubMed
  238. Cabet E, et al. 2015. PLoS One. 10: 0137009. PubMed
  239. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  240. Dong G, et al. 2020. Haematologica. . PubMed
  241. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  242. Bahl S, et al. 2021. Cell Death Dis. 12:469. PubMed
  243. Soon P, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
  244. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  245. Su PL, et al. 2021. Lung Cancer. 158:137. PubMed
  246. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  247. Sun T, et al. 2019. Cancer Med. 2.457638889. PubMed
  248. Marton N, et al. 2017. Cellular and Molecular Life Sciences. 10.1007/s00018-017-2535-8. PubMed
  249. Kranz P, et al. 2017. Cell Death Dis. 8:e2986. PubMed
  250. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  251. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  252. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  253. Guentsch A, et al. 2017. Mol Cell Biol. 37: e00236-16. PubMed
  254. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  255. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  256. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  257. Kojima N, et al. 2015. Anticancer Res. 35: 4493-4499. PubMed
  258. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
  259. Kim J, et al. 2019. PLoS One. 14:e0210248. PubMed
  260. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  261. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  262. Jatho A, et al. 2022. Cells. 11:. PubMed
  263. Sahin E, et al. 2014. J Immunol. 193:1717. PubMed
  264. Vancsik T, et al. 2019. Cancer Med. 3.313888889. PubMed
  265. Vulin M, et al. 2022. Oncogene. :. PubMed
  266. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  267. Langsch S, et al. 2016. Cancer Res . 76: 4160 - 4169. PubMed
  268. Xiong R, et al. 2015. Mol Pharmacol. 88: 1045 - 1054. PubMed

Antigen Details

Gene ID
NA

Related FAQs

How is your Annexin made and what sequence does it cover?

It is made in E. coli, covering human aa Met1-Asp320.

How does pH and staining temperature affect Annexin V-Phosphatidylserine binding?

Annexin-Phosphatidylserine binding is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.

Why do I need to use Annexin V Binding Buffer?

Annexin V binding requires the presence of calcium in the solution.  So, we provide Annexin V Binding Buffer (cat # 422201), which is optimized for the best performance of Annexin V staining.

Can I use RPMI during Annexin V staining?

It is best to follow protocol as described on the product data sheet. Moreover, RPMI 1640 has a relatively high concentration of phosphate and low calcium ion concentration, which negatively impacts Annexin binding to its target phosphatidylserine (PS). Measurement of cell death by using Annexin V may also be significantly affected by time of incubation on ice, calcium concentration, and type of medium.

Can I freeze Annexin V conjugates?

It should not be frozen as it will lead to loss of biological activity due to dimerization.

Is Annexin V suitable for conjugation with the Maxpar® kit for CyTOF®?

Maxpar® Labeling kits require the protein to be partially reduced, so the metal chelate can be introduced through an SH group in the hinge region of the reduced antibody. Human Annexin V contains only one Cysteine which was reported to be chemically inactive. Thus, the Maxpar® labeling protocol would not work with Annexin V, unless a free –SH group can be introduced to Annexin V.  For more information regarding SH-mediated conjugation of Annexin V please consult published papers such as this one.

Go To Top Version: 5    Revision Date: 03-15-2021

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