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Brilliant Violet 421™ Streptavidin Brilliant Violet 421™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 421™
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Product Citations
publications
SAV_BV421_071911
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
  • SAV_BV421_071911
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
405225 100 µg SEK3881
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405226 100 µL SEK1622
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 421™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 421™ by 405 nm laser light induces a fluorescence maximum emission of 421 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Cat. No. 405225: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Cat. No. 405226: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
405225 is bottled at 0.5 mg/ml.
405226 is bottled at 0.1 mg/ml.
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 421™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 421™ conjugation. For immunofluorescent staining, we recommend using Cat. No. 405226 at dilutions ≤ 0.015 µg in 100 µL staining volume per million cells. For applications requiring high concentration of streptavidin reagent such as tetramer labeling, we recommend using Cat. No. 405225. It is recommended that the reagent be titrated for optimal performance for each application.

* Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Streptavidin-Brilliant Violet 421™ is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Brilliant Violet 421™ is 340 kD and Streptavidin alone is 52 kD.

Additional Product Notes

View more applications data using this product in immunofluorescence microscopy and labeling tetramers in flow cytometry in our Scientific Poster Library.

Application References
  1. Kim G, et al. 2013. J. Immunol. 190:1510. PubMed.
  2. Giltiay NV, Et al. 2013. J. Immunol. 191:2155. PubMed
  3. Sugiyama D, et al. 2013. PNAS. 110:17945. PubMed
  4. Scally SW, et al. 2013. J. Exp Med. 210:2569. PubMed
  5. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  6. Higdon LE, et al. 2014. PNAS. 111:5652. PubMed
  7. Friedman RS, et al. 2014. PNAS. 111:9223. PubMed
  8. van de Weijer ML, et al. 2014. Nat Commun. 5:3832. PubMed
  9. Mise-Omata S, et al. 2014. Int Immunol. PubMed
  10. Choo JA, et al. 2014. J Virol. 88:10613. PubMed
  11. Xing YL, et al. 2014. J Neurosci. 34:14128. PubMed
  12. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  13. Poh Cm, et al. 2014. Infect Immun. 82:4854. PubMed
  14. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  15. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  16. Krishnaswamy JK, et al. 2015. PNAS. 112:3056. PubMed
  17. Rampuria P, et al. 2015. Int Immunol. 27:253. PubMed
Product Citations
  1. Takizawa F, et al. 2016. J Immunol. 196: 4522 - 4535. PubMed
  2. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  3. He T, et al. 2022. Liver Int. 42:1287. PubMed
  4. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  5. Wolf EW, et al. 2022. Proc Natl Acad Sci U S A. 119:e2201794119. PubMed
  6. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  7. Lei X, et al. 2022. J Exp Med. 219: . PubMed
  8. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  9. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  10. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  11. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  12. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  13. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  14. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  15. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  16. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  17. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  18. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  19. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  20. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  21. Sun P, et al. 2022. iScience. 25:105202. PubMed
  22. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  23. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  24. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  25. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  26. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  27. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  28. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  29. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  30. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  31. Sigmund EC, et al. 2023. PLoS One. 18:e0285597. PubMed
  32. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  33. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  34. Steines L, et al. 2021. Front Immunol. 12:657894. PubMed
  35. García-Alegria E, et al. 2021. STAR Protoc. 2:100367. PubMed
  36. Angkasekwinai P, et al. 2017. PLoS One. 10.1371/journal.pone.0184684. PubMed
  37. van Eeden C, et al. 2018. Cell Rep. 25:3123. PubMed
  38. Reyes RA, et al. 2021. PLoS One. 16:e0261656. PubMed
  39. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  40. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  41. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  42. Parra–Sánchez H, et al. 2019. Viruses. 1.153472222. PubMed
  43. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  44. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  45. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  46. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  47. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  48. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  49. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  50. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  51. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  52. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  53. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  54. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  55. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  56. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  57. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  58. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  59. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  60. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  61. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  62. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  63. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  64. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  65. Im S, et al. 2016. Nature. 537:417-421. PubMed
  66. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  67. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  68. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  69. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  70. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  71. Koay H, et al. 2016. Nat Immunol. 10.1038/ni.3565. PubMed
  72. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  73. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  74. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  75. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  76. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  77. Krishnaswamy J, et al. 2015. Proc Natl Acad Sci U S A. 112:3056. PubMed
  78. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  79. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  80. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  81. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  82. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  83. Bähr A, et al. 2016. PLoS One. 11: 0155676. PubMed
  84. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  85. Liu B, et al. 2015. J Immunol. 194:3583. PubMed
  86. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  87. Tarke A, et al. 2022. Cell. 185:847. PubMed
  88. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  89. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  90. Cavazzoni CB, et al. 2022. Cell Rep. 38:110399. PubMed
  91. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  92. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  93. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  94. Weijer M, et al. 2014. Nat Commun. 5:3832. PubMed
  95. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  96. Sanjo H, et al. 2015. PLoS One. 10: 0128761. PubMed
  97. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  98. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  99. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  100. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  101. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  102. Borroto A, et al. 2022. Front Immunol. 13:799919. PubMed
  103. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  104. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  105. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  106. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  107. Scally S, et al. 2013. J Exp Med. 210:2569. PubMed
  108. Blomberg R, et al. 2019. Matrix Biol. 83:60. PubMed
  109. Claudepierre M, et al. 2014. J Virol. 88:5242. PubMed
  110. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  111. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  112. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  113. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  114. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  115. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  116. Guelen L, et al. 2022. J Immunother Cancer. 10:. PubMed
  117. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  118. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  119. Dan JM, et al. 2021. Science. :371. PubMed
  120. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  121. Lederer K, et al. 2022. Cell. . PubMed
  122. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  123. Lang P 2015. Int Immunol. 27:253. PubMed
  124. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  125. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  126. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
  127. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  128. Fries CN, et al. 2021. Sci Rep. 11:14494. PubMed
  129. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  130. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  131. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  132. Clement RL, et al. 2019. Nat Immunol. 20:1360. PubMed
  133. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  134. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  135. Wells DK, et al. 2020. Cell. 183:818. PubMed
  136. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  137. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  138. Choo J, et al. 2014. J Virol. 88:10613. PubMed
  139. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  140. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  141. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  142. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  143. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  144. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
  145. Binnewies M, et al. 2019. Cell. 177:556. PubMed
  146. Cho A, et al. 2022. J Exp Med. 219:. PubMed
  147. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  148. Giri T, et al. 2022. Heliyon. 8:e09407. PubMed
  149. Takizawa F, et al. 2016. J Immunol. 196: 4522 - 4535. PubMed
  150. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  151. He T, et al. 2022. Liver Int. 42:1287. PubMed
  152. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  153. Wolf EW, et al. 2022. Proc Natl Acad Sci U S A. 119:e2201794119. PubMed
  154. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  155. Lei X, et al. 2022. J Exp Med. 219: . PubMed
  156. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  157. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  158. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  159. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  160. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  161. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  162. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  163. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  164. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  165. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  166. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  167. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  168. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  169. Sun P, et al. 2022. iScience. 25:105202. PubMed
  170. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  171. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  172. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  173. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  174. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  175. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  176. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  177. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  178. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  179. Sigmund EC, et al. 2023. PLoS One. 18:e0285597. PubMed
  180. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  181. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  182. Steines L, et al. 2021. Front Immunol. 12:657894. PubMed
  183. García-Alegria E, et al. 2021. STAR Protoc. 2:100367. PubMed
  184. Angkasekwinai P, et al. 2017. PLoS One. 10.1371/journal.pone.0184684. PubMed
  185. van Eeden C, et al. 2018. Cell Rep. 25:3123. PubMed
  186. Reyes RA, et al. 2021. PLoS One. 16:e0261656. PubMed
  187. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  188. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  189. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  190. Parra–Sánchez H, et al. 2019. Viruses. 1.153472222. PubMed
  191. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  192. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  193. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  194. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  195. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  196. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  197. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  198. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  199. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  200. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  201. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  202. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  203. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  204. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  205. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  206. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  207. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  208. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  209. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  210. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  211. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  212. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  213. Im S, et al. 2016. Nature. 537:417-421. PubMed
  214. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  215. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  216. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  217. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  218. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  219. Koay H, et al. 2016. Nat Immunol. 10.1038/ni.3565. PubMed
  220. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  221. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  222. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  223. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  224. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  225. Krishnaswamy J, et al. 2015. Proc Natl Acad Sci U S A. 112:3056. PubMed
  226. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  227. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  228. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  229. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  230. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  231. Bähr A, et al. 2016. PLoS One. 11: 0155676. PubMed
  232. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  233. Liu B, et al. 2015. J Immunol. 194:3583. PubMed
  234. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  235. Tarke A, et al. 2022. Cell. 185:847. PubMed
  236. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  237. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  238. Cavazzoni CB, et al. 2022. Cell Rep. 38:110399. PubMed
  239. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  240. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  241. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  242. Weijer M, et al. 2014. Nat Commun. 5:3832. PubMed
  243. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  244. Sanjo H, et al. 2015. PLoS One. 10: 0128761. PubMed
  245. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  246. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  247. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  248. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  249. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  250. Borroto A, et al. 2022. Front Immunol. 13:799919. PubMed
  251. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  252. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  253. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  254. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  255. Scally S, et al. 2013. J Exp Med. 210:2569. PubMed
  256. Blomberg R, et al. 2019. Matrix Biol. 83:60. PubMed
  257. Claudepierre M, et al. 2014. J Virol. 88:5242. PubMed
  258. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  259. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  260. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  261. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  262. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  263. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  264. Guelen L, et al. 2022. J Immunother Cancer. 10:. PubMed
  265. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  266. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  267. Dan JM, et al. 2021. Science. :371. PubMed
  268. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  269. Lederer K, et al. 2022. Cell. . PubMed
  270. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  271. Lang P 2015. Int Immunol. 27:253. PubMed
  272. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  273. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  274. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
  275. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  276. Fries CN, et al. 2021. Sci Rep. 11:14494. PubMed
  277. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  278. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  279. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  280. Clement RL, et al. 2019. Nat Immunol. 20:1360. PubMed
  281. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  282. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  283. Wells DK, et al. 2020. Cell. 183:818. PubMed
  284. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  285. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  286. Choo J, et al. 2014. J Virol. 88:10613. PubMed
  287. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  288. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  289. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  290. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  291. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  292. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
  293. Binnewies M, et al. 2019. Cell. 177:556. PubMed
  294. Cho A, et al. 2022. J Exp Med. 219:. PubMed
  295. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  296. Giri T, et al. 2022. Heliyon. 8:e09407. PubMed

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 8    Revision Date: 12-18-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 421™
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Product Citations
publications
SAV_BV421_071911
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
  • SAV_BV421_071911
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
405225 100 µg SEK3881
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405226 100 µL SEK1622
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 421™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 421™ by 405 nm laser light induces a fluorescence maximum emission of 421 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Cat. No. 405225: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Cat. No. 405226: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
405225 is bottled at 0.5 mg/ml.
405226 is bottled at 0.1 mg/ml.
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 421™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 421™ conjugation. For immunofluorescent staining, we recommend using Cat. No. 405226 at dilutions ≤ 0.015 µg in 100 µL staining volume per million cells. For applications requiring high concentration of streptavidin reagent such as tetramer labeling, we recommend using Cat. No. 405225. It is recommended that the reagent be titrated for optimal performance for each application.

* Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Streptavidin-Brilliant Violet 421™ is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Brilliant Violet 421™ is 340 kD and Streptavidin alone is 52 kD.

Additional Product Notes

View more applications data using this product in immunofluorescence microscopy and labeling tetramers in flow cytometry in our Scientific Poster Library.

Application References
  1. Kim G, et al. 2013. J. Immunol. 190:1510. PubMed.
  2. Giltiay NV, Et al. 2013. J. Immunol. 191:2155. PubMed
  3. Sugiyama D, et al. 2013. PNAS. 110:17945. PubMed
  4. Scally SW, et al. 2013. J. Exp Med. 210:2569. PubMed
  5. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  6. Higdon LE, et al. 2014. PNAS. 111:5652. PubMed
  7. Friedman RS, et al. 2014. PNAS. 111:9223. PubMed
  8. van de Weijer ML, et al. 2014. Nat Commun. 5:3832. PubMed
  9. Mise-Omata S, et al. 2014. Int Immunol. PubMed
  10. Choo JA, et al. 2014. J Virol. 88:10613. PubMed
  11. Xing YL, et al. 2014. J Neurosci. 34:14128. PubMed
  12. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  13. Poh Cm, et al. 2014. Infect Immun. 82:4854. PubMed
  14. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  15. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  16. Krishnaswamy JK, et al. 2015. PNAS. 112:3056. PubMed
  17. Rampuria P, et al. 2015. Int Immunol. 27:253. PubMed
Product Citations
  1. Takizawa F, et al. 2016. J Immunol. 196: 4522 - 4535. PubMed
  2. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  3. He T, et al. 2022. Liver Int. 42:1287. PubMed
  4. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  5. Wolf EW, et al. 2022. Proc Natl Acad Sci U S A. 119:e2201794119. PubMed
  6. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  7. Lei X, et al. 2022. J Exp Med. 219: . PubMed
  8. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  9. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  10. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  11. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  12. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  13. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  14. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  15. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  16. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  17. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  18. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  19. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  20. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  21. Sun P, et al. 2022. iScience. 25:105202. PubMed
  22. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  23. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  24. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  25. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  26. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  27. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  28. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  29. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  30. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  31. Sigmund EC, et al. 2023. PLoS One. 18:e0285597. PubMed
  32. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  33. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  34. Steines L, et al. 2021. Front Immunol. 12:657894. PubMed
  35. García-Alegria E, et al. 2021. STAR Protoc. 2:100367. PubMed
  36. Angkasekwinai P, et al. 2017. PLoS One. 10.1371/journal.pone.0184684. PubMed
  37. van Eeden C, et al. 2018. Cell Rep. 25:3123. PubMed
  38. Reyes RA, et al. 2021. PLoS One. 16:e0261656. PubMed
  39. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  40. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  41. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  42. Parra–Sánchez H, et al. 2019. Viruses. 1.153472222. PubMed
  43. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  44. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  45. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  46. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  47. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  48. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  49. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  50. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  51. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  52. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  53. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  54. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  55. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  56. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  57. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  58. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  59. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  60. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  61. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  62. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  63. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  64. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  65. Im S, et al. 2016. Nature. 537:417-421. PubMed
  66. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  67. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  68. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  69. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  70. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  71. Koay H, et al. 2016. Nat Immunol. 10.1038/ni.3565. PubMed
  72. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  73. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  74. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  75. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  76. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  77. Krishnaswamy J, et al. 2015. Proc Natl Acad Sci U S A. 112:3056. PubMed
  78. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  79. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  80. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  81. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  82. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  83. Bähr A, et al. 2016. PLoS One. 11: 0155676. PubMed
  84. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  85. Liu B, et al. 2015. J Immunol. 194:3583. PubMed
  86. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  87. Tarke A, et al. 2022. Cell. 185:847. PubMed
  88. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  89. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  90. Cavazzoni CB, et al. 2022. Cell Rep. 38:110399. PubMed
  91. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  92. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  93. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  94. Weijer M, et al. 2014. Nat Commun. 5:3832. PubMed
  95. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  96. Sanjo H, et al. 2015. PLoS One. 10: 0128761. PubMed
  97. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  98. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  99. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  100. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  101. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  102. Borroto A, et al. 2022. Front Immunol. 13:799919. PubMed
  103. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  104. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  105. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  106. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  107. Scally S, et al. 2013. J Exp Med. 210:2569. PubMed
  108. Blomberg R, et al. 2019. Matrix Biol. 83:60. PubMed
  109. Claudepierre M, et al. 2014. J Virol. 88:5242. PubMed
  110. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  111. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  112. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  113. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  114. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  115. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  116. Guelen L, et al. 2022. J Immunother Cancer. 10:. PubMed
  117. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  118. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  119. Dan JM, et al. 2021. Science. :371. PubMed
  120. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  121. Lederer K, et al. 2022. Cell. . PubMed
  122. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  123. Lang P 2015. Int Immunol. 27:253. PubMed
  124. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  125. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  126. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
  127. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  128. Fries CN, et al. 2021. Sci Rep. 11:14494. PubMed
  129. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  130. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  131. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  132. Clement RL, et al. 2019. Nat Immunol. 20:1360. PubMed
  133. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  134. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  135. Wells DK, et al. 2020. Cell. 183:818. PubMed
  136. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  137. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  138. Choo J, et al. 2014. J Virol. 88:10613. PubMed
  139. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  140. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  141. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  142. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  143. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  144. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
  145. Binnewies M, et al. 2019. Cell. 177:556. PubMed
  146. Cho A, et al. 2022. J Exp Med. 219:. PubMed
  147. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  148. Giri T, et al. 2022. Heliyon. 8:e09407. PubMed
  149. Takizawa F, et al. 2016. J Immunol. 196: 4522 - 4535. PubMed
  150. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  151. He T, et al. 2022. Liver Int. 42:1287. PubMed
  152. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  153. Wolf EW, et al. 2022. Proc Natl Acad Sci U S A. 119:e2201794119. PubMed
  154. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  155. Lei X, et al. 2022. J Exp Med. 219: . PubMed
  156. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  157. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  158. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  159. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  160. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  161. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  162. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  163. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  164. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  165. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  166. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  167. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  168. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  169. Sun P, et al. 2022. iScience. 25:105202. PubMed
  170. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  171. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  172. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  173. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  174. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  175. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  176. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  177. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  178. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  179. Sigmund EC, et al. 2023. PLoS One. 18:e0285597. PubMed
  180. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  181. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  182. Steines L, et al. 2021. Front Immunol. 12:657894. PubMed
  183. García-Alegria E, et al. 2021. STAR Protoc. 2:100367. PubMed
  184. Angkasekwinai P, et al. 2017. PLoS One. 10.1371/journal.pone.0184684. PubMed
  185. van Eeden C, et al. 2018. Cell Rep. 25:3123. PubMed
  186. Reyes RA, et al. 2021. PLoS One. 16:e0261656. PubMed
  187. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  188. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  189. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  190. Parra–Sánchez H, et al. 2019. Viruses. 1.153472222. PubMed
  191. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  192. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  193. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  194. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  195. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  196. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  197. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  198. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  199. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  200. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  201. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  202. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  203. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  204. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  205. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  206. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  207. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  208. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  209. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  210. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  211. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  212. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  213. Im S, et al. 2016. Nature. 537:417-421. PubMed
  214. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  215. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  216. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  217. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  218. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  219. Koay H, et al. 2016. Nat Immunol. 10.1038/ni.3565. PubMed
  220. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  221. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  222. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  223. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  224. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  225. Krishnaswamy J, et al. 2015. Proc Natl Acad Sci U S A. 112:3056. PubMed
  226. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  227. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  228. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  229. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  230. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  231. Bähr A, et al. 2016. PLoS One. 11: 0155676. PubMed
  232. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  233. Liu B, et al. 2015. J Immunol. 194:3583. PubMed
  234. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  235. Tarke A, et al. 2022. Cell. 185:847. PubMed
  236. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  237. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  238. Cavazzoni CB, et al. 2022. Cell Rep. 38:110399. PubMed
  239. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  240. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  241. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  242. Weijer M, et al. 2014. Nat Commun. 5:3832. PubMed
  243. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  244. Sanjo H, et al. 2015. PLoS One. 10: 0128761. PubMed
  245. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  246. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  247. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  248. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  249. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  250. Borroto A, et al. 2022. Front Immunol. 13:799919. PubMed
  251. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  252. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  253. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  254. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  255. Scally S, et al. 2013. J Exp Med. 210:2569. PubMed
  256. Blomberg R, et al. 2019. Matrix Biol. 83:60. PubMed
  257. Claudepierre M, et al. 2014. J Virol. 88:5242. PubMed
  258. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  259. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  260. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  261. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  262. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  263. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  264. Guelen L, et al. 2022. J Immunother Cancer. 10:. PubMed
  265. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  266. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  267. Dan JM, et al. 2021. Science. :371. PubMed
  268. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  269. Lederer K, et al. 2022. Cell. . PubMed
  270. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  271. Lang P 2015. Int Immunol. 27:253. PubMed
  272. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  273. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  274. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
  275. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  276. Fries CN, et al. 2021. Sci Rep. 11:14494. PubMed
  277. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  278. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  279. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  280. Clement RL, et al. 2019. Nat Immunol. 20:1360. PubMed
  281. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  282. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  283. Wells DK, et al. 2020. Cell. 183:818. PubMed
  284. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  285. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  286. Choo J, et al. 2014. J Virol. 88:10613. PubMed
  287. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  288. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  289. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  290. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  291. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  292. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
  293. Binnewies M, et al. 2019. Cell. 177:556. PubMed
  294. Cho A, et al. 2022. J Exp Med. 219:. PubMed
  295. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  296. Giri T, et al. 2022. Heliyon. 8:e09407. PubMed

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 8    Revision Date: 12-18-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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