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Brilliant Violet 650™ anti-mouse CD8a Antibody Brilliant Violet 650™ anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-6.7_BV605_013112
C57BL/6 mouse splenocytes were stained with CD3 PE and CD8a (clone 53-6.7) Brilliant Violet 650™.
  • 53-6.7_BV605_013112
    C57BL/6 mouse splenocytes were stained with CD3 PE and CD8a (clone 53-6.7) Brilliant Violet 650™.
Compare all formats See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
100742 50 µg SEK2294
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Request Bulk Quote
100741 125 µL SEK1756
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Harapas CR, et al. 2022. Sci Immunol. 7:eabi4611. PubMed
  2. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  3. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  4. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  5. Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed
  6. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  7. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  8. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  9. Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed
  10. Gary EN, et al. 2022. Cell Rep Med. 3:100693. PubMed
  11. Klaus T, et al. 2022. JCI Insight. 7: . PubMed
  12. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  13. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  14. Sylvester MA, et al. 2022. Am J Physiol Renal Physiol. 322:F245. PubMed
  15. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  16. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  17. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  18. Kloepper J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4476-4481. PubMed
  19. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  20. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  21. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  22. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  23. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  24. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  25. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  26. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  27. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  28. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
  29. Kumar V, et al. 2022. Front Cardiovasc Med. 9:992653. PubMed
  30. Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed
  31. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  32. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  33. Kumar S, et al. 2021. Cancer Discov. 11:2050. PubMed
  34. Linnerbauer M, et al. 2022. Front Immunol. 12:800128. PubMed
  35. Marangoni F, et al. 2021. Cell. . PubMed
  36. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  37. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  38. Gary EN, et al. 2021. iScience. 24(7):102699. PubMed
  39. MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
  40. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  41. Sidwell T, et al. 2020. Nat Commun. 0.633333333. PubMed
  42. McAndrews KM, et al. 2021. Oncogene. 40:4440. PubMed
  43. Dokoshi T, et al. 2021. J Clin Invest. 131:. PubMed
  44. Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed
  45. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  46. Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
  47. Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed
  48. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  49. Carter TJ, et al. 2021. Small. 17:e2005241. PubMed
  50. Walton ZE et al. 2018. Cell. 174(1):72-87 . PubMed
  51. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  52. Port JR, et al. 2020. J Virol. 94:. PubMed
  53. Chiang HY, et al. 2022. Nat Commun. 13:874. PubMed
  54. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  55. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  56. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  57. Herrera R, et al. 2015. Infect Immun . 83: 3771 - 3780.. PubMed
  58. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  59. Śledzińska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed
  60. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  61. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  62. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  63. McAndrews KM, et al. 2021. J Biol Chem. 296:100523. PubMed
  64. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  65. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  66. Shi H, et al. 2020. Immunity. 51(6):1012-1027.e7.. PubMed
  67. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  68. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  69. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  70. Silva DA, et al. 2019. Nature. 565:186. PubMed
  71. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  72. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  73. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  74. Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
  75. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  76. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  77. Goc J, et al. 2021. Cell. 184:5015. PubMed
  78. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  79. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  80. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  81. Deets KA, et al. 2021. Elife. 10:. PubMed
  82. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  83. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  84. Harapas CR, et al. 2022. Sci Immunol. 7:eabi4611. PubMed
  85. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  86. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  87. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  88. Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed
  89. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  90. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  91. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  92. Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed
  93. Gary EN, et al. 2022. Cell Rep Med. 3:100693. PubMed
  94. Klaus T, et al. 2022. JCI Insight. 7: . PubMed
  95. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  96. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  97. Sylvester MA, et al. 2022. Am J Physiol Renal Physiol. 322:F245. PubMed
  98. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  99. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  100. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  101. Kloepper J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4476-4481. PubMed
  102. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  103. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  104. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  105. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  106. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  107. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  108. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  109. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  110. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  111. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
  112. Kumar V, et al. 2022. Front Cardiovasc Med. 9:992653. PubMed
  113. Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed
  114. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  115. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  116. Kumar S, et al. 2021. Cancer Discov. 11:2050. PubMed
  117. Linnerbauer M, et al. 2022. Front Immunol. 12:800128. PubMed
  118. Marangoni F, et al. 2021. Cell. . PubMed
  119. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  120. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  121. Gary EN, et al. 2021. iScience. 24(7):102699. PubMed
  122. MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
  123. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  124. Sidwell T, et al. 2020. Nat Commun. 0.633333333. PubMed
  125. McAndrews KM, et al. 2021. Oncogene. 40:4440. PubMed
  126. Dokoshi T, et al. 2021. J Clin Invest. 131:. PubMed
  127. Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed
  128. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  129. Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
  130. Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed
  131. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  132. Carter TJ, et al. 2021. Small. 17:e2005241. PubMed
  133. Walton ZE et al. 2018. Cell. 174(1):72-87 . PubMed
  134. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  135. Port JR, et al. 2020. J Virol. 94:. PubMed
  136. Chiang HY, et al. 2022. Nat Commun. 13:874. PubMed
  137. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  138. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  139. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  140. Herrera R, et al. 2015. Infect Immun . 83: 3771 - 3780.. PubMed
  141. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  142. Śledzińska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed
  143. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  144. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  145. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  146. McAndrews KM, et al. 2021. J Biol Chem. 296:100523. PubMed
  147. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  148. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  149. Shi H, et al. 2020. Immunity. 51(6):1012-1027.e7.. PubMed
  150. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  151. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  152. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  153. Silva DA, et al. 2019. Nature. 565:186. PubMed
  154. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  155. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  156. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  157. Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
  158. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  159. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  160. Goc J, et al. 2021. Cell. 184:5015. PubMed
  161. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  162. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  163. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  164. Deets KA, et al. 2021. Elife. 10:. PubMed
  165. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  166. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID
AB_2563056 (BioLegend Cat. No. 100742)
AB_11124344 (BioLegend Cat. No. 100741)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD8a 53-6.7 FC
FITC anti-mouse CD8a 53-6.7 FC
PE anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC-F,IP
PE/Cyanine5 anti-mouse CD8a 53-6.7 FC
Biotin anti-mouse CD8a 53-6.7 FC,IHC
PE/Cyanine7 anti-mouse CD8a 53-6.7 FC
PE/Dazzle™ 594 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 750 anti-mouse CD8a 53-6.7 FC
GoInVivo™ Purified anti-mouse CD8a 53-6.7 FC
TotalSeq™-A0002 anti-mouse CD8a 53-6.7 PG
Spark Blue™ 550 anti-mouse CD8a 53-6.7 FC
Spark NIR™ 685 anti-mouse CD8a 53-6.7 FC
TotalSeq™-C0002 anti-mouse CD8a 53-6.7 PG
TotalSeq™-B0002 anti-mouse CD8a 53-6.7 PG
Spark YG™ 570 anti-mouse CD8a 53-6.7 IHC-F
PE/Fire™ 640 anti-mouse CD8a 53-6.7 FC
PE/Fire™ 700 anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 488 anti-mouse CD8a 53-6.7 FC,3D IHC
APC/Cyanine7 anti-mouse CD8a 53-6.7 FC
Pacific Blue™ anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 647 anti-mouse CD8a 53-6.7 FC,IHC-F,3D IHC,SB
Alexa Fluor® 700 anti-mouse CD8a 53-6.7 FC
PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 FC
PerCP anti-mouse CD8a 53-6.7 FC
Brilliant Violet 650™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 605™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 421™ anti-mouse CD8a 53-6.7 FC,IHC,SB
Brilliant Violet 711™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 570™ anti-mouse CD8a 53-6.7 FC
Ultra-LEAF™ Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 785™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 510™ anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a (Maxpar® Ready) 53-6.7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a 53-6.7 IHC-F,FC,3D IHC
Spark Blue™ 574 anti-mouse CD8a Antibody 53-6.7 FC
Spark Violet™ 423 anti-mouse CD8a Antibody 53-6.7 FC
Spark UV™ 387 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 515 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
PE/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark PLUS UV395™ anti-mouse CD8a 53-6.7 FC
PerCP/Fire™ 780 anti-mouse CD8a 53-6.7 FC
Spark YG™ 581 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
Spark YG™ 593 anti-mouse CD8a (Flexi-Fluor™) Antibody 53-6.7 FC
Spark PLUS V475™ anti-mouse CD8a 53-6.7 FC
StarBright UltraViolet 795 anti-mouse CD8a 53-6.7 FC
StarBright UltraViolet 575 anti-mouse CD8a 53-6.7 FC
Go To Top Version: 2    Revision Date: 01-21-2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
53-6.7_BV605_013112
C57BL/6 mouse splenocytes were stained with CD3 PE and CD8a (clone 53-6.7) Brilliant Violet 650™.
  • 53-6.7_BV605_013112
    C57BL/6 mouse splenocytes were stained with CD3 PE and CD8a (clone 53-6.7) Brilliant Violet 650™.
Compare all formats See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
100742 50 µg SEK2294
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100741 125 µL SEK1756
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Harapas CR, et al. 2022. Sci Immunol. 7:eabi4611. PubMed
  2. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  3. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  4. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  5. Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed
  6. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  7. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  8. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  9. Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed
  10. Gary EN, et al. 2022. Cell Rep Med. 3:100693. PubMed
  11. Klaus T, et al. 2022. JCI Insight. 7: . PubMed
  12. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  13. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  14. Sylvester MA, et al. 2022. Am J Physiol Renal Physiol. 322:F245. PubMed
  15. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  16. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  17. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  18. Kloepper J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4476-4481. PubMed
  19. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  20. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  21. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  22. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  23. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  24. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  25. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  26. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  27. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  28. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
  29. Kumar V, et al. 2022. Front Cardiovasc Med. 9:992653. PubMed
  30. Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed
  31. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  32. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  33. Kumar S, et al. 2021. Cancer Discov. 11:2050. PubMed
  34. Linnerbauer M, et al. 2022. Front Immunol. 12:800128. PubMed
  35. Marangoni F, et al. 2021. Cell. . PubMed
  36. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  37. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  38. Gary EN, et al. 2021. iScience. 24(7):102699. PubMed
  39. MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
  40. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  41. Sidwell T, et al. 2020. Nat Commun. 0.633333333. PubMed
  42. McAndrews KM, et al. 2021. Oncogene. 40:4440. PubMed
  43. Dokoshi T, et al. 2021. J Clin Invest. 131:. PubMed
  44. Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed
  45. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  46. Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
  47. Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed
  48. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  49. Carter TJ, et al. 2021. Small. 17:e2005241. PubMed
  50. Walton ZE et al. 2018. Cell. 174(1):72-87 . PubMed
  51. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  52. Port JR, et al. 2020. J Virol. 94:. PubMed
  53. Chiang HY, et al. 2022. Nat Commun. 13:874. PubMed
  54. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  55. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  56. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  57. Herrera R, et al. 2015. Infect Immun . 83: 3771 - 3780.. PubMed
  58. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  59. Śledzińska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed
  60. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  61. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  62. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  63. McAndrews KM, et al. 2021. J Biol Chem. 296:100523. PubMed
  64. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  65. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  66. Shi H, et al. 2020. Immunity. 51(6):1012-1027.e7.. PubMed
  67. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  68. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  69. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  70. Silva DA, et al. 2019. Nature. 565:186. PubMed
  71. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  72. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  73. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  74. Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
  75. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  76. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  77. Goc J, et al. 2021. Cell. 184:5015. PubMed
  78. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  79. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  80. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  81. Deets KA, et al. 2021. Elife. 10:. PubMed
  82. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  83. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  84. Harapas CR, et al. 2022. Sci Immunol. 7:eabi4611. PubMed
  85. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  86. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  87. Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed
  88. Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed
  89. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  90. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  91. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  92. Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed
  93. Gary EN, et al. 2022. Cell Rep Med. 3:100693. PubMed
  94. Klaus T, et al. 2022. JCI Insight. 7: . PubMed
  95. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  96. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  97. Sylvester MA, et al. 2022. Am J Physiol Renal Physiol. 322:F245. PubMed
  98. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  99. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  100. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  101. Kloepper J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4476-4481. PubMed
  102. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  103. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  104. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  105. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  106. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  107. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  108. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  109. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  110. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  111. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
  112. Kumar V, et al. 2022. Front Cardiovasc Med. 9:992653. PubMed
  113. Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed
  114. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  115. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  116. Kumar S, et al. 2021. Cancer Discov. 11:2050. PubMed
  117. Linnerbauer M, et al. 2022. Front Immunol. 12:800128. PubMed
  118. Marangoni F, et al. 2021. Cell. . PubMed
  119. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  120. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  121. Gary EN, et al. 2021. iScience. 24(7):102699. PubMed
  122. MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
  123. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  124. Sidwell T, et al. 2020. Nat Commun. 0.633333333. PubMed
  125. McAndrews KM, et al. 2021. Oncogene. 40:4440. PubMed
  126. Dokoshi T, et al. 2021. J Clin Invest. 131:. PubMed
  127. Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed
  128. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  129. Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
  130. Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed
  131. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  132. Carter TJ, et al. 2021. Small. 17:e2005241. PubMed
  133. Walton ZE et al. 2018. Cell. 174(1):72-87 . PubMed
  134. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  135. Port JR, et al. 2020. J Virol. 94:. PubMed
  136. Chiang HY, et al. 2022. Nat Commun. 13:874. PubMed
  137. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  138. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  139. Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed
  140. Herrera R, et al. 2015. Infect Immun . 83: 3771 - 3780.. PubMed
  141. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  142. Śledzińska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed
  143. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  144. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  145. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  146. McAndrews KM, et al. 2021. J Biol Chem. 296:100523. PubMed
  147. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  148. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  149. Shi H, et al. 2020. Immunity. 51(6):1012-1027.e7.. PubMed
  150. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  151. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  152. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  153. Silva DA, et al. 2019. Nature. 565:186. PubMed
  154. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  155. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  156. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  157. Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
  158. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  159. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  160. Goc J, et al. 2021. Cell. 184:5015. PubMed
  161. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  162. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  163. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  164. Deets KA, et al. 2021. Elife. 10:. PubMed
  165. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  166. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID
AB_2563056 (BioLegend Cat. No. 100742)
AB_11124344 (BioLegend Cat. No. 100741)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD8a 53-6.7 FC
FITC anti-mouse CD8a 53-6.7 FC
PE anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC-F,IP
PE/Cyanine5 anti-mouse CD8a 53-6.7 FC
Biotin anti-mouse CD8a 53-6.7 FC,IHC
PE/Cyanine7 anti-mouse CD8a 53-6.7 FC
PE/Dazzle™ 594 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 750 anti-mouse CD8a 53-6.7 FC
GoInVivo™ Purified anti-mouse CD8a 53-6.7 FC
TotalSeq™-A0002 anti-mouse CD8a 53-6.7 PG
Spark Blue™ 550 anti-mouse CD8a 53-6.7 FC
Spark NIR™ 685 anti-mouse CD8a 53-6.7 FC
TotalSeq™-C0002 anti-mouse CD8a 53-6.7 PG
TotalSeq™-B0002 anti-mouse CD8a 53-6.7 PG
Spark YG™ 570 anti-mouse CD8a 53-6.7 IHC-F
PE/Fire™ 640 anti-mouse CD8a 53-6.7 FC
PE/Fire™ 700 anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 488 anti-mouse CD8a 53-6.7 FC,3D IHC
APC/Cyanine7 anti-mouse CD8a 53-6.7 FC
Pacific Blue™ anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 647 anti-mouse CD8a 53-6.7 FC,IHC-F,3D IHC,SB
Alexa Fluor® 700 anti-mouse CD8a 53-6.7 FC
PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 FC
PerCP anti-mouse CD8a 53-6.7 FC
Brilliant Violet 650™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 605™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 421™ anti-mouse CD8a 53-6.7 FC,IHC,SB
Brilliant Violet 711™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 570™ anti-mouse CD8a 53-6.7 FC
Ultra-LEAF™ Purified anti-mouse CD8a 53-6.7 FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 785™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 510™ anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a (Maxpar® Ready) 53-6.7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a 53-6.7 IHC-F,FC,3D IHC
Spark Blue™ 574 anti-mouse CD8a Antibody 53-6.7 FC
Spark Violet™ 423 anti-mouse CD8a Antibody 53-6.7 FC
Spark UV™ 387 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 515 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
PE/Fire™ 810 anti-mouse CD8a 53-6.7 FC
Spark PLUS UV395™ anti-mouse CD8a 53-6.7 FC
PerCP/Fire™ 780 anti-mouse CD8a 53-6.7 FC
Spark YG™ 581 anti-mouse CD8a (Flexi-Fluor™) 53-6.7 FC
Spark YG™ 593 anti-mouse CD8a (Flexi-Fluor™) Antibody 53-6.7 FC
Spark PLUS V475™ anti-mouse CD8a 53-6.7 FC
StarBright UltraViolet 795 anti-mouse CD8a 53-6.7 FC
StarBright UltraViolet 575 anti-mouse CD8a 53-6.7 FC
Go To Top Version: 2    Revision Date: 01-21-2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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