HRP Donkey anti-rabbit IgG (minimal x-reactivity) Antibody HRP Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
406401 500 µL SEK1185
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from donkey antiserum by rabbit immunoglobulin affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expirations date of your lot of product
Application

WB - Quality tested
ELISA, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Hunt D, et al. 2018. PLoS One. 13:e0200764. PubMed
  2. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  3. Shukla S, et al. 2022. FASEB J. 36: . PubMed
  4. Wang L, et al. 2022. Viruses. 14: . PubMed
  5. Biering SB, et al. 2022. Nat Commun. 13:7630. PubMed
  6. Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed
  7. Zanuttigh E, et al. 2023. Pharmaceutics. 15: . PubMed
  8. Apte S, et al. 2023. Sci Adv. 9:eade1851. PubMed
  9. Evans JG, et al. 2023. Viruses. 15:. PubMed
  10. Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed
  11. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
  12. Miki H, et al. 2020. J Immunol. 204:753. PubMed
  13. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  14. Nitiss JL, et al. 2021. Curr Protoc. 1:e250. PubMed
  15. Zech M, et al. 2018. Cold Spring Harb Mol Case Stud. 4:. PubMed
  16. Adkar-Purushothama C, et al. 2015. Plant Cell. 27: 2178-2194. PubMed
  17. Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed
  18. Lam DD, et al. 2022. J Neurosci. 42:1557. PubMed
  19. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  20. Xu H, et al. 2021. Microb Cell Fact. 20:115. PubMed
  21. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
  22. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  23. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  24. Wang Y, et al. 2016. Cell Death Dis. 7:e2458. PubMed
  25. Lee K, et al. 2020. Biology (Basel). 9:00. PubMed
  26. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  27. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
  28. Ohta E, et al. 2011. FEBS Lett. 585:2165. PubMed
  29. Saiz ML, et al. 2021. Clin Epigenetics. 13:187. PubMed
  30. Chan TC, et al. 2021. Clin Transl Med. 11:e674. PubMed
  31. Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed
  32. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  33. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  34. Ashley CL, et al. 2019. Viruses. 11:3. PubMed
  35. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  36. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  37. Kiianitsa K, et al. 2020. DNA Repair (Amst). 96:102977. PubMed
  38. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  39. Kauke MJ, et al. 2018. Mol Cancer Ther. 1.939583333. PubMed
  40. Yu C, et al. 2021. Front Genet. 12:689902. PubMed
  41. Moffat J, et al. 2013. Vaccine. 31:2310. PubMed
  42. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  43. Kiianitsa K, et al. 2021. J Leukoc Biol. 110:829. PubMed
  44. Luis-Garcia ER, et al. 2021. Int J Mol Sci. 22:. PubMed
  45. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  46. Veronica Margarecaesha Anyndita N, et al. 2018. Heliyon. 4:e00564. PubMed
  47. Hunt D, et al. 2018. PLoS One. 13:e0200764. PubMed
  48. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  49. Shukla S, et al. 2022. FASEB J. 36: . PubMed
  50. Wang L, et al. 2022. Viruses. 14: . PubMed
  51. Biering SB, et al. 2022. Nat Commun. 13:7630. PubMed
  52. Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed
  53. Zanuttigh E, et al. 2023. Pharmaceutics. 15: . PubMed
  54. Apte S, et al. 2023. Sci Adv. 9:eade1851. PubMed
  55. Evans JG, et al. 2023. Viruses. 15:. PubMed
  56. Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed
  57. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
  58. Miki H, et al. 2020. J Immunol. 204:753. PubMed
  59. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  60. Nitiss JL, et al. 2021. Curr Protoc. 1:e250. PubMed
  61. Zech M, et al. 2018. Cold Spring Harb Mol Case Stud. 4:. PubMed
  62. Adkar-Purushothama C, et al. 2015. Plant Cell. 27: 2178-2194. PubMed
  63. Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed
  64. Lam DD, et al. 2022. J Neurosci. 42:1557. PubMed
  65. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  66. Xu H, et al. 2021. Microb Cell Fact. 20:115. PubMed
  67. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
  68. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  69. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  70. Wang Y, et al. 2016. Cell Death Dis. 7:e2458. PubMed
  71. Lee K, et al. 2020. Biology (Basel). 9:00. PubMed
  72. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  73. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
  74. Ohta E, et al. 2011. FEBS Lett. 585:2165. PubMed
  75. Saiz ML, et al. 2021. Clin Epigenetics. 13:187. PubMed
  76. Chan TC, et al. 2021. Clin Transl Med. 11:e674. PubMed
  77. Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed
  78. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  79. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  80. Ashley CL, et al. 2019. Viruses. 11:3. PubMed
  81. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  82. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  83. Kiianitsa K, et al. 2020. DNA Repair (Amst). 96:102977. PubMed
  84. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  85. Kauke MJ, et al. 2018. Mol Cancer Ther. 1.939583333. PubMed
  86. Yu C, et al. 2021. Front Genet. 12:689902. PubMed
  87. Moffat J, et al. 2013. Vaccine. 31:2310. PubMed
  88. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  89. Kiianitsa K, et al. 2021. J Leukoc Biol. 110:829. PubMed
  90. Luis-Garcia ER, et al. 2021. Int J Mol Sci. 22:. PubMed
  91. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  92. Veronica Margarecaesha Anyndita N, et al. 2018. Heliyon. 4:e00564. PubMed
RRID
AB_2099368 (BioLegend Cat. No. 406401)

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 12-12-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
406401 500 µL SEK1185
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from donkey antiserum by rabbit immunoglobulin affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expirations date of your lot of product
Application

WB - Quality tested
ELISA, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Hunt D, et al. 2018. PLoS One. 13:e0200764. PubMed
  2. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  3. Shukla S, et al. 2022. FASEB J. 36: . PubMed
  4. Wang L, et al. 2022. Viruses. 14: . PubMed
  5. Biering SB, et al. 2022. Nat Commun. 13:7630. PubMed
  6. Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed
  7. Zanuttigh E, et al. 2023. Pharmaceutics. 15: . PubMed
  8. Apte S, et al. 2023. Sci Adv. 9:eade1851. PubMed
  9. Evans JG, et al. 2023. Viruses. 15:. PubMed
  10. Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed
  11. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
  12. Miki H, et al. 2020. J Immunol. 204:753. PubMed
  13. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  14. Nitiss JL, et al. 2021. Curr Protoc. 1:e250. PubMed
  15. Zech M, et al. 2018. Cold Spring Harb Mol Case Stud. 4:. PubMed
  16. Adkar-Purushothama C, et al. 2015. Plant Cell. 27: 2178-2194. PubMed
  17. Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed
  18. Lam DD, et al. 2022. J Neurosci. 42:1557. PubMed
  19. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  20. Xu H, et al. 2021. Microb Cell Fact. 20:115. PubMed
  21. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
  22. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  23. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  24. Wang Y, et al. 2016. Cell Death Dis. 7:e2458. PubMed
  25. Lee K, et al. 2020. Biology (Basel). 9:00. PubMed
  26. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  27. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
  28. Ohta E, et al. 2011. FEBS Lett. 585:2165. PubMed
  29. Saiz ML, et al. 2021. Clin Epigenetics. 13:187. PubMed
  30. Chan TC, et al. 2021. Clin Transl Med. 11:e674. PubMed
  31. Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed
  32. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  33. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  34. Ashley CL, et al. 2019. Viruses. 11:3. PubMed
  35. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  36. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  37. Kiianitsa K, et al. 2020. DNA Repair (Amst). 96:102977. PubMed
  38. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  39. Kauke MJ, et al. 2018. Mol Cancer Ther. 1.939583333. PubMed
  40. Yu C, et al. 2021. Front Genet. 12:689902. PubMed
  41. Moffat J, et al. 2013. Vaccine. 31:2310. PubMed
  42. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  43. Kiianitsa K, et al. 2021. J Leukoc Biol. 110:829. PubMed
  44. Luis-Garcia ER, et al. 2021. Int J Mol Sci. 22:. PubMed
  45. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  46. Veronica Margarecaesha Anyndita N, et al. 2018. Heliyon. 4:e00564. PubMed
  47. Hunt D, et al. 2018. PLoS One. 13:e0200764. PubMed
  48. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  49. Shukla S, et al. 2022. FASEB J. 36: . PubMed
  50. Wang L, et al. 2022. Viruses. 14: . PubMed
  51. Biering SB, et al. 2022. Nat Commun. 13:7630. PubMed
  52. Hsu J, et al. 2022. J Biol Chem. 299:102848. PubMed
  53. Zanuttigh E, et al. 2023. Pharmaceutics. 15: . PubMed
  54. Apte S, et al. 2023. Sci Adv. 9:eade1851. PubMed
  55. Evans JG, et al. 2023. Viruses. 15:. PubMed
  56. Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed
  57. Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed
  58. Miki H, et al. 2020. J Immunol. 204:753. PubMed
  59. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  60. Nitiss JL, et al. 2021. Curr Protoc. 1:e250. PubMed
  61. Zech M, et al. 2018. Cold Spring Harb Mol Case Stud. 4:. PubMed
  62. Adkar-Purushothama C, et al. 2015. Plant Cell. 27: 2178-2194. PubMed
  63. Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed
  64. Lam DD, et al. 2022. J Neurosci. 42:1557. PubMed
  65. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  66. Xu H, et al. 2021. Microb Cell Fact. 20:115. PubMed
  67. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
  68. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  69. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  70. Wang Y, et al. 2016. Cell Death Dis. 7:e2458. PubMed
  71. Lee K, et al. 2020. Biology (Basel). 9:00. PubMed
  72. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  73. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
  74. Ohta E, et al. 2011. FEBS Lett. 585:2165. PubMed
  75. Saiz ML, et al. 2021. Clin Epigenetics. 13:187. PubMed
  76. Chan TC, et al. 2021. Clin Transl Med. 11:e674. PubMed
  77. Ram AK, et al. 2020. World J Gastroenterol. 26:7131. PubMed
  78. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  79. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  80. Ashley CL, et al. 2019. Viruses. 11:3. PubMed
  81. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  82. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  83. Kiianitsa K, et al. 2020. DNA Repair (Amst). 96:102977. PubMed
  84. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  85. Kauke MJ, et al. 2018. Mol Cancer Ther. 1.939583333. PubMed
  86. Yu C, et al. 2021. Front Genet. 12:689902. PubMed
  87. Moffat J, et al. 2013. Vaccine. 31:2310. PubMed
  88. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  89. Kiianitsa K, et al. 2021. J Leukoc Biol. 110:829. PubMed
  90. Luis-Garcia ER, et al. 2021. Int J Mol Sci. 22:. PubMed
  91. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  92. Veronica Margarecaesha Anyndita N, et al. 2018. Heliyon. 4:e00564. PubMed
RRID
AB_2099368 (BioLegend Cat. No. 406401)
Go To Top Version: 4    Revision Date: 12-12-2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account