Loading...

Purified anti-ITPR1 Antibody Purified anti-ITPR1 Antibody

Pricing & Availability
Clone
W22192B (See other available formats)
Regulatory Status
RUO
Other Names
Inositol 1,4,5-trisphosphate-gated calcium channel (ITPR1), IP3 receptor isoform 1 (IP3R 1, InsP3R1), Inositol 1,4,5, trisphosphate receptor type 1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
W22192B_PURE_ITPR1_IHC-P_060925
IHC staining with Purified anti-ITPR1 (clone W22192B) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Purified anti-ITPR1 (clone W22192B) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • W22192B_PURE_ITPR1_IHC-P_060925
    IHC staining with Purified anti-ITPR1 (clone W22192B) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Purified anti-ITPR1 (clone W22192B) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
655551 25 µg SEK1428
Check Availability


Need larger quantities of this item?
Request Bulk Quote
655552 100 µg SEK3780
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ITPR1 is primarily involved in calcium signaling within cells. Located on the membrane of the endoplasmic reticulum (ER), it functions as a calcium channel. Upon binding to inositol 1,4,5-trisphosphate (IP3), a second messenger molecule, ITPR1 facilitates the release of calcium ions from the ER into the cytoplasm. This calcium release plays a crucial role in regulating various cellular processes, including muscle contraction, neurotransmitter release, and gene expression. Mutations in the ITPR1 gene are associated with neurological disorders such as spinocerebellar ataxia and Gillespie syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein located near the C terminus of human ITPR1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

This product (W22192B) is not recommended for use in Western Blot (WB) due to the presence of a non-specific band in addition to the expected band.

This product is not recommended for use in intracellular flow cytometry (ICFC) or immunocytochemistry (ICC).

RRID
AB_3699081 (BioLegend Cat. No. 655551)
AB_3699081 (BioLegend Cat. No. 655552)

Antigen Details

Structure
ITPR1 is a 2758 amino acid protein with a predicted molecular weight of 313.9 kD.
Distribution

Endoplasmic reticulum

Function
IP3-gated calcium channel
Interaction
Calmodulin, FKBP1A, Ankyrin-B, HOMER proteins
Ligand/Receptor
Inositol 1,4,5-trisphosphate (IP3)
Biology Area
Signal Transduction, Synaptic Biology
Molecular Family
Endoplasmic Reticulum Markers
Antigen References
  1. Tolonen JP, et al. 2024. Mov Disord. 39:141-151. 
  2. Putney JW, 1987. Am J Physiol. 252:G149-57. 
  3. van de Leemput J, et al. 2007. PLoS Genet. 3:e108.
Gene ID
3708 View all products for this Gene ID
UniProt
View information about ITPR1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06-09-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
W22192B (See other available formats)
Regulatory Status
RUO
Other Names
Inositol 1,4,5-trisphosphate-gated calcium channel (ITPR1), IP3 receptor isoform 1 (IP3R 1, InsP3R1), Inositol 1,4,5, trisphosphate receptor type 1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
W22192B_PURE_ITPR1_IHC-P_060925
IHC staining with Purified anti-ITPR1 (clone W22192B) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Purified anti-ITPR1 (clone W22192B) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • W22192B_PURE_ITPR1_IHC-P_060925
    IHC staining with Purified anti-ITPR1 (clone W22192B) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Purified anti-ITPR1 (clone W22192B) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
655551 25 µg SEK1428
Check Availability


Need larger quantities of this item?
Request Bulk Quote
655552 100 µg SEK3780
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ITPR1 is primarily involved in calcium signaling within cells. Located on the membrane of the endoplasmic reticulum (ER), it functions as a calcium channel. Upon binding to inositol 1,4,5-trisphosphate (IP3), a second messenger molecule, ITPR1 facilitates the release of calcium ions from the ER into the cytoplasm. This calcium release plays a crucial role in regulating various cellular processes, including muscle contraction, neurotransmitter release, and gene expression. Mutations in the ITPR1 gene are associated with neurological disorders such as spinocerebellar ataxia and Gillespie syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein located near the C terminus of human ITPR1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

This product (W22192B) is not recommended for use in Western Blot (WB) due to the presence of a non-specific band in addition to the expected band.

This product is not recommended for use in intracellular flow cytometry (ICFC) or immunocytochemistry (ICC).

RRID
AB_3699081 (BioLegend Cat. No. 655551)
AB_3699081 (BioLegend Cat. No. 655552)

Antigen Details

Structure
ITPR1 is a 2758 amino acid protein with a predicted molecular weight of 313.9 kD.
Distribution

Endoplasmic reticulum

Function
IP3-gated calcium channel
Interaction
Calmodulin, FKBP1A, Ankyrin-B, HOMER proteins
Ligand/Receptor
Inositol 1,4,5-trisphosphate (IP3)
Biology Area
Signal Transduction, Synaptic Biology
Molecular Family
Endoplasmic Reticulum Markers
Antigen References
  1. Tolonen JP, et al. 2024. Mov Disord. 39:141-151. 
  2. Putney JW, 1987. Am J Physiol. 252:G149-57. 
  3. van de Leemput J, et al. 2007. PLoS Genet. 3:e108.
Gene ID
3708 View all products for this Gene ID
UniProt
View information about ITPR1 on UniProt.org
Go To Top Version: 1    Revision Date: 06-09-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account