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Purified anti-mouse CD28 Antibody Purified anti-mouse CD28 Antibody

Pricing & Availability
Clone
37.51 (See other available formats)
Regulatory Status
RUO
Other Names
Tp44, T44
Isotype
Syrian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
37.51
C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
  • 37.51
    C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
Compare all formats
Cat # Size Price Quantity Check Availability Save
102101 50 µg SEK454
Check Availability


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Request Bulk Quote
102102 500 µg SEK1067
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Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD28 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, Costim, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).

Application References
  1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
  2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
  3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
  4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
  5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
  6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
  8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
  9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
  10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  11. Barbi J, et al. 2007. Blood 110:2215.
  12. Milpied P, et al. 2011. Blood 118:2993. PubMed
  13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
  14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
  15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
  16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Akiyama C, et al. 2019. J Immunol. 203:835. PubMed
  2. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  3. Oh H, et al. 2017. Immunity. 47:450. PubMed
  4. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  5. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  6. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  7. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  8. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  9. Campisi L, et al. 2022. Nature. 606:945. PubMed
  10. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
  11. Liu W, et al. 2022. Stem Cells Int. 2022:1052166. PubMed
  12. Zhu M, et al. 2022. Front Immunol. 13:1019248. PubMed
  13. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  14. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  15. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  16. Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
  17. Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed
  18. Kim H, et al. 2023. Bone Res. 11:17. PubMed
  19. Anto NP, et al. 2023. Front Immunol. 14:1126464. PubMed
  20. Aydin S, et al. 2023. Nat Commun. 14:3106. PubMed
  21. Cross EW, et al. 2019. PLoS One. 14:e0211446. PubMed
  22. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  23. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
  24. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  25. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  26. Yamada T, et al. 2019. J Immunol. 202:1088. PubMed
  27. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  28. He N 2005. J Immunol. 174:6967. PubMed
  29. Crispin J, et al. 2012. J Immunol. 188:3567. PubMed
  30. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  31. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  32. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  33. Busnelli M, et al. 2022. Arterioscler Thromb Vasc Biol. 42:839. PubMed
  34. Milpied P, et al. 2011. Blood. 118:2993. PubMed
  35. Sanctuary MR, et al. 2019. Mucosal Immunol. 0.638888889. PubMed
  36. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
  37. Oghumu S, et al. 2019. J Immunol. 203:789. PubMed
  38. Kitamura T, et al. 2018. Front Immunol. 8:2004. PubMed
  39. Lu SX, et al. 2021. Cell. . PubMed
  40. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  41. Macchi C, et al. 2022. iScience. 25:104435. PubMed
  42. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  43. Marangoni F, et al. 2021. Cell. . PubMed
  44. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  45. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  46. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  47. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  48. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  49. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  50. Tuzlak S, et al. 2017. Cell Death Differ. 24:523. PubMed
  51. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  52. Rao TN, et al. 2020. J Immunol. 204:2600. PubMed
  53. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  54. Perchonock C, et al. 2006. Mol Cell Biol . 26:6005. PubMed
  55. Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed
  56. Kölle J, et al. 2022. iScience. 25:104440. PubMed
  57. Hanoteau A, et al. 2019. J Immunother Cancer. 7:10. PubMed
  58. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  59. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  60. Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed
  61. Rosenberger C, et al. 2008. Proc Natl Acad Sci U S A. 105:2544. PubMed
  62. McCully M, et al. 2015. J Immunol. 195: 96 - 104. PubMed
  63. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed
  64. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  65. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  66. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  67. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  68. Racioppi L, et al. 2019. Nat Commun. 10:2450. PubMed
  69. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
  70. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  71. Scholz T, et al. 2017. PLoS One. 12:e0182646. PubMed
  72. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  73. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  74. Otsuka S, et al. 2021. J Clin Invest. 131:. PubMed
  75. Thumkeo D, et al. 2020. Sci Adv. 6:eaay2432. PubMed
  76. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  77. Hao Y, et al. 2018. Front Immunol. 9:1127. PubMed
  78. Samanta D, et al. 2020. Cell Rep. 108073:32. PubMed
  79. Hollmann C, et al. 2016. J Immunol. 197: 3130 - 3141. PubMed
  80. Li Z, et al. 2021. MBio. 12:e0254221. PubMed
  81. Zhang S, et al. 2021. Front Immunol. 12:635076. PubMed
  82. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  83. Majchrzak K, et al. 2017. JCI Insight. 2:e90547. PubMed
  84. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
  85. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
  86. Endo Y, et al. 2015. Cell Rep. 12: 1042-1055. PubMed
  87. Sripada A, et al. 2021. PLoS Biol. 19:e3001063. PubMed
  88. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  89. Wu Y, et al. 2021. J Clin Invest. 131:. PubMed
  90. Schreiber K, et al. 2020. Cancer Immunol Res. 8:192. PubMed
  91. Schiering C, et al. 2017. Nature. 542:242. PubMed
  92. Lee J, et al. 2020. Cells. 9:. PubMed
  93. Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed
  94. Link MA, et al. 2017. PLoS One. 12:e0184320. PubMed
  95. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  96. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  97. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  98. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  99. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  100. Guo J, et al. 2020. Cell Mol Immunol. 1222:17. PubMed
  101. Sun Y, et al. 2021. Nat Commun. 12:5147. PubMed
  102. Cohen MA, et al. 2020. Cell Stem Cell. 26(4):579-592. PubMed
  103. He N 2005. J Exp Med. 202:395. PubMed
  104. Cunningham N, et al. 2011. Int Immunol. 1.439583333. PubMed
  105. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  106. Perchonock C, et al. 2007. J Immunol. 179:1768. PubMed
  107. Abe Y, et al. 2017. Biochem Biophys Rep. 0.543055556. PubMed
  108. He Y, et al. 2021. eLife. 10:00. PubMed
  109. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  110. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  111. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
  112. Wang W, et al. 2022. Nat Commun. 13:4495. PubMed
  113. Grinberg–Bleyer Y, et al. 2017. Cell. 170:1096. PubMed
  114. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  115. Plaza-Sirvent C, et al. 2017. Cell Rep. 18(1):12-22. PubMed
  116. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  117. Chen YH, et al. 2022. Arthritis Res Ther. 24:27. PubMed
  118. Xia L, et al. 2021. Cancer Immunol Res. 9:707. PubMed
  119. Akiyama C, et al. 2019. J Immunol. 203:835. PubMed
  120. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  121. Oh H, et al. 2017. Immunity. 47:450. PubMed
  122. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  123. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  124. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  125. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  126. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  127. Campisi L, et al. 2022. Nature. 606:945. PubMed
  128. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
  129. Liu W, et al. 2022. Stem Cells Int. 2022:1052166. PubMed
  130. Zhu M, et al. 2022. Front Immunol. 13:1019248. PubMed
  131. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  132. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  133. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  134. Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
  135. Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed
  136. Kim H, et al. 2023. Bone Res. 11:17. PubMed
  137. Anto NP, et al. 2023. Front Immunol. 14:1126464. PubMed
  138. Aydin S, et al. 2023. Nat Commun. 14:3106. PubMed
  139. Cross EW, et al. 2019. PLoS One. 14:e0211446. PubMed
  140. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  141. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
  142. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  143. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  144. Yamada T, et al. 2019. J Immunol. 202:1088. PubMed
  145. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  146. He N 2005. J Immunol. 174:6967. PubMed
  147. Crispin J, et al. 2012. J Immunol. 188:3567. PubMed
  148. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  149. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  150. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  151. Busnelli M, et al. 2022. Arterioscler Thromb Vasc Biol. 42:839. PubMed
  152. Milpied P, et al. 2011. Blood. 118:2993. PubMed
  153. Sanctuary MR, et al. 2019. Mucosal Immunol. 0.638888889. PubMed
  154. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
  155. Oghumu S, et al. 2019. J Immunol. 203:789. PubMed
  156. Kitamura T, et al. 2018. Front Immunol. 8:2004. PubMed
  157. Lu SX, et al. 2021. Cell. . PubMed
  158. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  159. Macchi C, et al. 2022. iScience. 25:104435. PubMed
  160. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  161. Marangoni F, et al. 2021. Cell. . PubMed
  162. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  163. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  164. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  165. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  166. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  167. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  168. Tuzlak S, et al. 2017. Cell Death Differ. 24:523. PubMed
  169. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  170. Rao TN, et al. 2020. J Immunol. 204:2600. PubMed
  171. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  172. Perchonock C, et al. 2006. Mol Cell Biol . 26:6005. PubMed
  173. Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed
  174. Kölle J, et al. 2022. iScience. 25:104440. PubMed
  175. Hanoteau A, et al. 2019. J Immunother Cancer. 7:10. PubMed
  176. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  177. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  178. Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed
  179. Rosenberger C, et al. 2008. Proc Natl Acad Sci U S A. 105:2544. PubMed
  180. McCully M, et al. 2015. J Immunol. 195: 96 - 104. PubMed
  181. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed
  182. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  183. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  184. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  185. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  186. Racioppi L, et al. 2019. Nat Commun. 10:2450. PubMed
  187. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
  188. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  189. Scholz T, et al. 2017. PLoS One. 12:e0182646. PubMed
  190. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  191. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  192. Otsuka S, et al. 2021. J Clin Invest. 131:. PubMed
  193. Thumkeo D, et al. 2020. Sci Adv. 6:eaay2432. PubMed
  194. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  195. Hao Y, et al. 2018. Front Immunol. 9:1127. PubMed
  196. Samanta D, et al. 2020. Cell Rep. 108073:32. PubMed
  197. Hollmann C, et al. 2016. J Immunol. 197: 3130 - 3141. PubMed
  198. Li Z, et al. 2021. MBio. 12:e0254221. PubMed
  199. Zhang S, et al. 2021. Front Immunol. 12:635076. PubMed
  200. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  201. Majchrzak K, et al. 2017. JCI Insight. 2:e90547. PubMed
  202. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
  203. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
  204. Endo Y, et al. 2015. Cell Rep. 12: 1042-1055. PubMed
  205. Sripada A, et al. 2021. PLoS Biol. 19:e3001063. PubMed
  206. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  207. Wu Y, et al. 2021. J Clin Invest. 131:. PubMed
  208. Schreiber K, et al. 2020. Cancer Immunol Res. 8:192. PubMed
  209. Schiering C, et al. 2017. Nature. 542:242. PubMed
  210. Lee J, et al. 2020. Cells. 9:. PubMed
  211. Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed
  212. Link MA, et al. 2017. PLoS One. 12:e0184320. PubMed
  213. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  214. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  215. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  216. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  217. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  218. Guo J, et al. 2020. Cell Mol Immunol. 1222:17. PubMed
  219. Sun Y, et al. 2021. Nat Commun. 12:5147. PubMed
  220. Cohen MA, et al. 2020. Cell Stem Cell. 26(4):579-592. PubMed
  221. He N 2005. J Exp Med. 202:395. PubMed
  222. Cunningham N, et al. 2011. Int Immunol. 1.439583333. PubMed
  223. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  224. Perchonock C, et al. 2007. J Immunol. 179:1768. PubMed
  225. Abe Y, et al. 2017. Biochem Biophys Rep. 0.543055556. PubMed
  226. He Y, et al. 2021. eLife. 10:00. PubMed
  227. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  228. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  229. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
  230. Wang W, et al. 2022. Nat Commun. 13:4495. PubMed
  231. Grinberg–Bleyer Y, et al. 2017. Cell. 170:1096. PubMed
  232. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  233. Plaza-Sirvent C, et al. 2017. Cell Rep. 18(1):12-22. PubMed
  234. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  235. Chen YH, et al. 2022. Arthritis Res Ther. 24:27. PubMed
  236. Xia L, et al. 2021. Cancer Immunol Res. 9:707. PubMed
RRID
AB_312866 (BioLegend Cat. No. 102101)
AB_312867 (BioLegend Cat. No. 102102)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution

Thymocytes, CD4+, CD8+ peripheral T cells, NK cells

Function
Costimulates T and NK cells
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID
12487 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
37.51 (See other available formats)
Regulatory Status
RUO
Other Names
Tp44, T44
Isotype
Syrian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
37.51
C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
  • 37.51
    C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
Compare all formats
Cat # Size Price Quantity Check Availability Save
102101 50 µg SEK454
Check Availability


Need larger quantities of this item?
Request Bulk Quote
102102 500 µg SEK1067
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD28 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, Costim, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).

Application References
  1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
  2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
  3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
  4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
  5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
  6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
  8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
  9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
  10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  11. Barbi J, et al. 2007. Blood 110:2215.
  12. Milpied P, et al. 2011. Blood 118:2993. PubMed
  13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
  14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
  15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
  16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Akiyama C, et al. 2019. J Immunol. 203:835. PubMed
  2. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  3. Oh H, et al. 2017. Immunity. 47:450. PubMed
  4. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  5. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  6. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  7. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  8. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  9. Campisi L, et al. 2022. Nature. 606:945. PubMed
  10. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
  11. Liu W, et al. 2022. Stem Cells Int. 2022:1052166. PubMed
  12. Zhu M, et al. 2022. Front Immunol. 13:1019248. PubMed
  13. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  14. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  15. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  16. Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
  17. Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed
  18. Kim H, et al. 2023. Bone Res. 11:17. PubMed
  19. Anto NP, et al. 2023. Front Immunol. 14:1126464. PubMed
  20. Aydin S, et al. 2023. Nat Commun. 14:3106. PubMed
  21. Cross EW, et al. 2019. PLoS One. 14:e0211446. PubMed
  22. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  23. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
  24. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  25. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  26. Yamada T, et al. 2019. J Immunol. 202:1088. PubMed
  27. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  28. He N 2005. J Immunol. 174:6967. PubMed
  29. Crispin J, et al. 2012. J Immunol. 188:3567. PubMed
  30. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  31. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  32. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  33. Busnelli M, et al. 2022. Arterioscler Thromb Vasc Biol. 42:839. PubMed
  34. Milpied P, et al. 2011. Blood. 118:2993. PubMed
  35. Sanctuary MR, et al. 2019. Mucosal Immunol. 0.638888889. PubMed
  36. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
  37. Oghumu S, et al. 2019. J Immunol. 203:789. PubMed
  38. Kitamura T, et al. 2018. Front Immunol. 8:2004. PubMed
  39. Lu SX, et al. 2021. Cell. . PubMed
  40. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  41. Macchi C, et al. 2022. iScience. 25:104435. PubMed
  42. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  43. Marangoni F, et al. 2021. Cell. . PubMed
  44. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  45. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  46. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  47. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  48. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  49. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  50. Tuzlak S, et al. 2017. Cell Death Differ. 24:523. PubMed
  51. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  52. Rao TN, et al. 2020. J Immunol. 204:2600. PubMed
  53. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  54. Perchonock C, et al. 2006. Mol Cell Biol . 26:6005. PubMed
  55. Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed
  56. Kölle J, et al. 2022. iScience. 25:104440. PubMed
  57. Hanoteau A, et al. 2019. J Immunother Cancer. 7:10. PubMed
  58. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  59. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  60. Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed
  61. Rosenberger C, et al. 2008. Proc Natl Acad Sci U S A. 105:2544. PubMed
  62. McCully M, et al. 2015. J Immunol. 195: 96 - 104. PubMed
  63. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed
  64. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  65. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  66. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  67. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  68. Racioppi L, et al. 2019. Nat Commun. 10:2450. PubMed
  69. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
  70. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  71. Scholz T, et al. 2017. PLoS One. 12:e0182646. PubMed
  72. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  73. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  74. Otsuka S, et al. 2021. J Clin Invest. 131:. PubMed
  75. Thumkeo D, et al. 2020. Sci Adv. 6:eaay2432. PubMed
  76. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  77. Hao Y, et al. 2018. Front Immunol. 9:1127. PubMed
  78. Samanta D, et al. 2020. Cell Rep. 108073:32. PubMed
  79. Hollmann C, et al. 2016. J Immunol. 197: 3130 - 3141. PubMed
  80. Li Z, et al. 2021. MBio. 12:e0254221. PubMed
  81. Zhang S, et al. 2021. Front Immunol. 12:635076. PubMed
  82. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  83. Majchrzak K, et al. 2017. JCI Insight. 2:e90547. PubMed
  84. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
  85. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
  86. Endo Y, et al. 2015. Cell Rep. 12: 1042-1055. PubMed
  87. Sripada A, et al. 2021. PLoS Biol. 19:e3001063. PubMed
  88. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  89. Wu Y, et al. 2021. J Clin Invest. 131:. PubMed
  90. Schreiber K, et al. 2020. Cancer Immunol Res. 8:192. PubMed
  91. Schiering C, et al. 2017. Nature. 542:242. PubMed
  92. Lee J, et al. 2020. Cells. 9:. PubMed
  93. Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed
  94. Link MA, et al. 2017. PLoS One. 12:e0184320. PubMed
  95. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  96. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  97. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  98. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  99. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  100. Guo J, et al. 2020. Cell Mol Immunol. 1222:17. PubMed
  101. Sun Y, et al. 2021. Nat Commun. 12:5147. PubMed
  102. Cohen MA, et al. 2020. Cell Stem Cell. 26(4):579-592. PubMed
  103. He N 2005. J Exp Med. 202:395. PubMed
  104. Cunningham N, et al. 2011. Int Immunol. 1.439583333. PubMed
  105. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  106. Perchonock C, et al. 2007. J Immunol. 179:1768. PubMed
  107. Abe Y, et al. 2017. Biochem Biophys Rep. 0.543055556. PubMed
  108. He Y, et al. 2021. eLife. 10:00. PubMed
  109. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  110. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  111. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
  112. Wang W, et al. 2022. Nat Commun. 13:4495. PubMed
  113. Grinberg–Bleyer Y, et al. 2017. Cell. 170:1096. PubMed
  114. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  115. Plaza-Sirvent C, et al. 2017. Cell Rep. 18(1):12-22. PubMed
  116. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  117. Chen YH, et al. 2022. Arthritis Res Ther. 24:27. PubMed
  118. Xia L, et al. 2021. Cancer Immunol Res. 9:707. PubMed
  119. Akiyama C, et al. 2019. J Immunol. 203:835. PubMed
  120. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  121. Oh H, et al. 2017. Immunity. 47:450. PubMed
  122. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  123. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  124. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  125. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  126. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  127. Campisi L, et al. 2022. Nature. 606:945. PubMed
  128. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
  129. Liu W, et al. 2022. Stem Cells Int. 2022:1052166. PubMed
  130. Zhu M, et al. 2022. Front Immunol. 13:1019248. PubMed
  131. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  132. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  133. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  134. Li T, et al. 2022. J Leukoc Biol. 112:845. PubMed
  135. Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed
  136. Kim H, et al. 2023. Bone Res. 11:17. PubMed
  137. Anto NP, et al. 2023. Front Immunol. 14:1126464. PubMed
  138. Aydin S, et al. 2023. Nat Commun. 14:3106. PubMed
  139. Cross EW, et al. 2019. PLoS One. 14:e0211446. PubMed
  140. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  141. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
  142. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  143. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  144. Yamada T, et al. 2019. J Immunol. 202:1088. PubMed
  145. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  146. He N 2005. J Immunol. 174:6967. PubMed
  147. Crispin J, et al. 2012. J Immunol. 188:3567. PubMed
  148. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  149. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  150. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  151. Busnelli M, et al. 2022. Arterioscler Thromb Vasc Biol. 42:839. PubMed
  152. Milpied P, et al. 2011. Blood. 118:2993. PubMed
  153. Sanctuary MR, et al. 2019. Mucosal Immunol. 0.638888889. PubMed
  154. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
  155. Oghumu S, et al. 2019. J Immunol. 203:789. PubMed
  156. Kitamura T, et al. 2018. Front Immunol. 8:2004. PubMed
  157. Lu SX, et al. 2021. Cell. . PubMed
  158. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  159. Macchi C, et al. 2022. iScience. 25:104435. PubMed
  160. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  161. Marangoni F, et al. 2021. Cell. . PubMed
  162. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  163. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  164. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  165. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  166. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  167. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  168. Tuzlak S, et al. 2017. Cell Death Differ. 24:523. PubMed
  169. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  170. Rao TN, et al. 2020. J Immunol. 204:2600. PubMed
  171. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  172. Perchonock C, et al. 2006. Mol Cell Biol . 26:6005. PubMed
  173. Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed
  174. Kölle J, et al. 2022. iScience. 25:104440. PubMed
  175. Hanoteau A, et al. 2019. J Immunother Cancer. 7:10. PubMed
  176. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  177. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  178. Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed
  179. Rosenberger C, et al. 2008. Proc Natl Acad Sci U S A. 105:2544. PubMed
  180. McCully M, et al. 2015. J Immunol. 195: 96 - 104. PubMed
  181. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed
  182. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  183. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  184. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  185. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  186. Racioppi L, et al. 2019. Nat Commun. 10:2450. PubMed
  187. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
  188. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  189. Scholz T, et al. 2017. PLoS One. 12:e0182646. PubMed
  190. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  191. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  192. Otsuka S, et al. 2021. J Clin Invest. 131:. PubMed
  193. Thumkeo D, et al. 2020. Sci Adv. 6:eaay2432. PubMed
  194. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  195. Hao Y, et al. 2018. Front Immunol. 9:1127. PubMed
  196. Samanta D, et al. 2020. Cell Rep. 108073:32. PubMed
  197. Hollmann C, et al. 2016. J Immunol. 197: 3130 - 3141. PubMed
  198. Li Z, et al. 2021. MBio. 12:e0254221. PubMed
  199. Zhang S, et al. 2021. Front Immunol. 12:635076. PubMed
  200. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  201. Majchrzak K, et al. 2017. JCI Insight. 2:e90547. PubMed
  202. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
  203. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
  204. Endo Y, et al. 2015. Cell Rep. 12: 1042-1055. PubMed
  205. Sripada A, et al. 2021. PLoS Biol. 19:e3001063. PubMed
  206. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  207. Wu Y, et al. 2021. J Clin Invest. 131:. PubMed
  208. Schreiber K, et al. 2020. Cancer Immunol Res. 8:192. PubMed
  209. Schiering C, et al. 2017. Nature. 542:242. PubMed
  210. Lee J, et al. 2020. Cells. 9:. PubMed
  211. Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed
  212. Link MA, et al. 2017. PLoS One. 12:e0184320. PubMed
  213. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  214. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  215. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  216. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  217. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  218. Guo J, et al. 2020. Cell Mol Immunol. 1222:17. PubMed
  219. Sun Y, et al. 2021. Nat Commun. 12:5147. PubMed
  220. Cohen MA, et al. 2020. Cell Stem Cell. 26(4):579-592. PubMed
  221. He N 2005. J Exp Med. 202:395. PubMed
  222. Cunningham N, et al. 2011. Int Immunol. 1.439583333. PubMed
  223. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  224. Perchonock C, et al. 2007. J Immunol. 179:1768. PubMed
  225. Abe Y, et al. 2017. Biochem Biophys Rep. 0.543055556. PubMed
  226. He Y, et al. 2021. eLife. 10:00. PubMed
  227. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  228. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  229. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
  230. Wang W, et al. 2022. Nat Commun. 13:4495. PubMed
  231. Grinberg–Bleyer Y, et al. 2017. Cell. 170:1096. PubMed
  232. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  233. Plaza-Sirvent C, et al. 2017. Cell Rep. 18(1):12-22. PubMed
  234. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  235. Chen YH, et al. 2022. Arthritis Res Ther. 24:27. PubMed
  236. Xia L, et al. 2021. Cancer Immunol Res. 9:707. PubMed
RRID
AB_312866 (BioLegend Cat. No. 102101)
AB_312867 (BioLegend Cat. No. 102102)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution

Thymocytes, CD4+, CD8+ peripheral T cells, NK cells

Function
Costimulates T and NK cells
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID
12487 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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