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True-Nuclear™ Transcription Factor Buffer Set True-Nuclear™ Transcription Factor Buffer Set

Pricing & Availability
Regulatory Status
RUO
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
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Product Citations
publications
Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
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424401 120 tests SEK1386
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Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
  1. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  2. Cook ME, et al. 2022. Sci Immunol. 7:eabo0981. PubMed
  3. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  4. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  5. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  6. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  7. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  8. Yi X, et al. 2023. Signal Transduct Target Ther. 8:107. PubMed
  9. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  10. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  11. Wu C, et al. 2023. Cancer Metab. 11:7. PubMed
  12. Adu-Berchie K, et al. 2023. Nat Commun. 14:3546. PubMed
  13. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  14. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  15. Manso BA, et al. 2021. STAR Protoc. 2:100485. PubMed
  16. Wu HJ, et al. 2021. Nat Commun. 12:4897. PubMed
  17. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  18. Douglass SM, et al. 2021. Cancer Res. 81:658. PubMed
  19. Capelle CM, et al. 2022. Immunology. 165:428. PubMed
  20. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  21. Grant NL, et al. 2022. Cell Rep. 39:110826. PubMed
  22. Hu M, et al. 2022. Int J Mol Sci. 23: . PubMed
  23. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  24. Yu H, et al. 2022. Hematol Oncol. 40:894. PubMed
  25. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  26. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  27. Noman MZ, et al. 2022. Front Immunol. 13:980704. PubMed
  28. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  29. Ishii T, et al. 2022. Cell Death Discov. 8:480. PubMed
  30. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  31. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  32. Landshammer A, et al. 2023. Elife. 12: . PubMed
  33. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  34. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  35. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  36. Zander R, et al. 2022. Immunity. 55:475. PubMed
  37. de Lemos L, et al. 2022. Development. 149:. PubMed
  38. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
  39. Zhang M, et al. 2020. Nat Commun. 4.707638889. PubMed
  40. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  41. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  42. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  43. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  44. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  45. Guo R, et al. 2020. Nat Microbiol. 0.938194444. PubMed
  46. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  47. Baram T, et al. 2021. Cells. 10:. PubMed
  48. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  49. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  50. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  51. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  52. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  53. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  54. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
  55. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  56. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  57. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  58. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  59. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  60. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  61. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  62. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  63. Wei SC, et al. 2020. Cancer Discov. . PubMed
  64. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  65. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  66. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  67. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  68. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  69. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  70. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  71. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  72. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  73. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  74. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  75. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  76. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  77. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  78. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  79. Woodruff M, et al. 2020. medRxiv. . PubMed
  80. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  81. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  82. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  83. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  84. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  85. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  86. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  87. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  88. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  89. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  90. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  91. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  92. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  93. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  94. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  95. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  96. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  97. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  98. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  99. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  100. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  101. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  102. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  103. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  104. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  105. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  106. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  107. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  108. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  109. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  110. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  111. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  112. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  113. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  114. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  115. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  116. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  117. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  118. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  119. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  120. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  121. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  122. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  123. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  124. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  125. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  126. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  127. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  128. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  129. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  130. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  131. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  132. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  133. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  134. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  135. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  136. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  137. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  138. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  139. Cook ME, et al. 2022. Sci Immunol. 7:eabo0981. PubMed
  140. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  141. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  142. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  143. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  144. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  145. Yi X, et al. 2023. Signal Transduct Target Ther. 8:107. PubMed
  146. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  147. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  148. Wu C, et al. 2023. Cancer Metab. 11:7. PubMed
  149. Adu-Berchie K, et al. 2023. Nat Commun. 14:3546. PubMed
  150. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  151. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  152. Manso BA, et al. 2021. STAR Protoc. 2:100485. PubMed
  153. Wu HJ, et al. 2021. Nat Commun. 12:4897. PubMed
  154. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  155. Douglass SM, et al. 2021. Cancer Res. 81:658. PubMed
  156. Capelle CM, et al. 2022. Immunology. 165:428. PubMed
  157. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  158. Grant NL, et al. 2022. Cell Rep. 39:110826. PubMed
  159. Hu M, et al. 2022. Int J Mol Sci. 23: . PubMed
  160. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  161. Yu H, et al. 2022. Hematol Oncol. 40:894. PubMed
  162. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  163. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  164. Noman MZ, et al. 2022. Front Immunol. 13:980704. PubMed
  165. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  166. Ishii T, et al. 2022. Cell Death Discov. 8:480. PubMed
  167. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  168. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  169. Landshammer A, et al. 2023. Elife. 12: . PubMed
  170. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  171. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  172. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  173. Zander R, et al. 2022. Immunity. 55:475. PubMed
  174. de Lemos L, et al. 2022. Development. 149:. PubMed
  175. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
  176. Zhang M, et al. 2020. Nat Commun. 4.707638889. PubMed
  177. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  178. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  179. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  180. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  181. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  182. Guo R, et al. 2020. Nat Microbiol. 0.938194444. PubMed
  183. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  184. Baram T, et al. 2021. Cells. 10:. PubMed
  185. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  186. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  187. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  188. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  189. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  190. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  191. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
  192. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  193. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  194. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  195. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  196. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  197. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  198. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  199. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  200. Wei SC, et al. 2020. Cancer Discov. . PubMed
  201. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  202. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  203. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  204. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  205. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  206. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  207. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  208. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  209. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  210. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  211. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  212. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  213. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  214. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  215. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  216. Woodruff M, et al. 2020. medRxiv. . PubMed
  217. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  218. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  219. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  220. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  221. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  222. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  223. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  224. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  225. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  226. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  227. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  228. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  229. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  230. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  231. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  232. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  233. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  234. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  235. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  236. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  237. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  238. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  239. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  240. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  241. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  242. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  243. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  244. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  245. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  246. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  247. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  248. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  249. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  250. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  251. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  252. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  253. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  254. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  255. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  256. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  257. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  258. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  259. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  260. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  261. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  262. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  263. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  264. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  265. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  266. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  267. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  268. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  269. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  270. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  271. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  272. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  273. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  274. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA

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Go To Top Version: 5    Revision Date: 05-20-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Pricing & Availability
Regulatory Status
RUO
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
Ave. Rating
Submit a Review
Product Citations
publications
Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
Cat # Size Price Quantity Check Availability Save
424401 120 tests SEK1386
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Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
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  186. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  187. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  188. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  189. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
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  191. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
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  193. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  194. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  195. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  196. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  197. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  198. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  199. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  200. Wei SC, et al. 2020. Cancer Discov. . PubMed
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  202. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  203. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  204. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  205. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  206. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  207. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  208. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  209. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  210. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  211. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  212. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  213. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
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  216. Woodruff M, et al. 2020. medRxiv. . PubMed
  217. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  218. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  219. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  220. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  221. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  222. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  223. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  224. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  225. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  226. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  227. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  228. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  229. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  230. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  231. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  232. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  233. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  234. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  235. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  236. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  237. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  238. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  239. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  240. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  241. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  242. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  243. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  244. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  245. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  246. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  247. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  248. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  249. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  250. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  251. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  252. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  253. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  254. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  255. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  256. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  257. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  258. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  259. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  260. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  261. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  262. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  263. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  264. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  265. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  266. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  267. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  268. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  269. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  270. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  271. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  272. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  273. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  274. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA
Go To Top Version: 5    Revision Date: 05-20-2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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