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APC anti-mouse CD326 (Ep-CAM) Antibody APC anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
G8dot8_APC_081908.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
  • G8dot8_APC_081908.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
118213 25 µg DKK606
Check Availability


Need larger quantities of this item?
Request Bulk Quote
118214 100 µg DKK1759
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  2. Elkon R, et al. 2015. Nat Commun. 6: 8549. PubMed
  3. Chen Q, et al. 2020. Cell Reports. 31(13):107828. PubMed
  4. Park SE, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1235. PubMed
  5. Na E, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L550. PubMed
  6. Xu J, et al. 2022. Dev Cell. 57:839. PubMed
  7. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  8. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
  9. Cui W, et al. 2023. J Exp Med. 220:. PubMed
  10. Kalgudde Gopal S, et al. 2023. Nat Commun. 14:3020. PubMed
  11. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  12. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
  13. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  14. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  15. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  16. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  17. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  18. Mevel R, et al. 2020. eLife. 9:00. PubMed
  19. Buerger S, et al. 2015. J Immunol. 195: 4106 - 4116. PubMed
  20. Malhotra D, et al. 2016. Nat Immunol. 17:187-195. PubMed
  21. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
  22. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  23. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  24. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  25. Ford MJ, et al. 2021. Cell Rep. 36:109677. PubMed
  26. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  27. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  28. Phung HT, et al. 2020. Immunohorizons. 0.25625. PubMed
  29. Chua CW, et al. 2018. Elife. 7. PubMed
  30. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  31. Semba T, et al. 2020. Cancers (Basel). 12:. PubMed
  32. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
  33. Willobee BA, et al. 2021. Mol Cancer Ther. 20:1246. PubMed
  34. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  35. Wang Y, et al. 2014. PLoS One. 9:113127. PubMed
  36. Katara GK, et al. 2020. Mol Oncol. 0.729861111. PubMed
  37. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  38. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  39. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  40. Rudnick JA, et al. 2021. Genes Dev. 35:963. PubMed
  41. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  42. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  43. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  44. Bar C, et al. 2020. Cell Reports. 28(1):257-266.e5.. PubMed
  45. Jiang M, et al. 2021. Developmental Cell. 56(11):1646-1660.e5. PubMed
  46. Sateriale A, et al. 2020. Cell Host & Microbe. 26(1):135-146.e5.. PubMed
  47. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  48. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  49. Ionkina AA, et al. 2021. Breast Cancer Res. 23:93. PubMed
  50. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  51. Hertzano R, et al. 2020. Laryngoscope. . PubMed
  52. Li J, et al. 2020. Development. :. PubMed
  53. Cerretelli G, et al. 2021. J Pathol. 255:464. PubMed
  54. Tang Z, et al. 2021. Stem Cell Res Ther. 12:397. PubMed
  55. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  56. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  57. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  58. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  59. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  60. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  61. Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed
  62. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  63. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  64. Caporarello N, et al. 2020. Aging Cell. 19:e13196. PubMed
  65. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  66. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  67. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  68. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  69. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  70. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  71. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  72. Tanimizu N, et al. 2014. J Biol Chem. 289:7859. PubMed
  73. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  74. Casey O, et al. 2012. PLoS One. 7:e41668. PubMed
  75. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  76. Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed
  77. Kasuga A, et al. 2020. Cancer Sci. . PubMed
  78. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  79. Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed
  80. Jadhav U, et al. 2019. Mol Cell. 74:542. PubMed
  81. Renz BW, et al. 2018. Cancer Cell. 33:75. PubMed
  82. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
  83. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  84. Chen Z, et al. 2020. Immunity. 54(1):151-163.e6. PubMed
  85. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  86. Elkon R, et al. 2015. Nat Commun. 6: 8549. PubMed
  87. Chen Q, et al. 2020. Cell Reports. 31(13):107828. PubMed
  88. Park SE, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1235. PubMed
  89. Na E, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L550. PubMed
  90. Xu J, et al. 2022. Dev Cell. 57:839. PubMed
  91. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  92. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
  93. Cui W, et al. 2023. J Exp Med. 220:. PubMed
  94. Kalgudde Gopal S, et al. 2023. Nat Commun. 14:3020. PubMed
  95. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  96. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
  97. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  98. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  99. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  100. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  101. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  102. Mevel R, et al. 2020. eLife. 9:00. PubMed
  103. Buerger S, et al. 2015. J Immunol. 195: 4106 - 4116. PubMed
  104. Malhotra D, et al. 2016. Nat Immunol. 17:187-195. PubMed
  105. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
  106. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  107. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  108. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  109. Ford MJ, et al. 2021. Cell Rep. 36:109677. PubMed
  110. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  111. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  112. Phung HT, et al. 2020. Immunohorizons. 0.25625. PubMed
  113. Chua CW, et al. 2018. Elife. 7. PubMed
  114. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  115. Semba T, et al. 2020. Cancers (Basel). 12:. PubMed
  116. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
  117. Willobee BA, et al. 2021. Mol Cancer Ther. 20:1246. PubMed
  118. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  119. Wang Y, et al. 2014. PLoS One. 9:113127. PubMed
  120. Katara GK, et al. 2020. Mol Oncol. 0.729861111. PubMed
  121. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  122. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  123. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  124. Rudnick JA, et al. 2021. Genes Dev. 35:963. PubMed
  125. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  126. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  127. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  128. Bar C, et al. 2020. Cell Reports. 28(1):257-266.e5.. PubMed
  129. Jiang M, et al. 2021. Developmental Cell. 56(11):1646-1660.e5. PubMed
  130. Sateriale A, et al. 2020. Cell Host & Microbe. 26(1):135-146.e5.. PubMed
  131. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  132. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  133. Ionkina AA, et al. 2021. Breast Cancer Res. 23:93. PubMed
  134. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  135. Hertzano R, et al. 2020. Laryngoscope. . PubMed
  136. Li J, et al. 2020. Development. :. PubMed
  137. Cerretelli G, et al. 2021. J Pathol. 255:464. PubMed
  138. Tang Z, et al. 2021. Stem Cell Res Ther. 12:397. PubMed
  139. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  140. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  141. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  142. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  143. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  144. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  145. Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed
  146. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  147. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  148. Caporarello N, et al. 2020. Aging Cell. 19:e13196. PubMed
  149. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  150. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  151. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  152. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  153. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  154. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  155. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  156. Tanimizu N, et al. 2014. J Biol Chem. 289:7859. PubMed
  157. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  158. Casey O, et al. 2012. PLoS One. 7:e41668. PubMed
  159. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  160. Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed
  161. Kasuga A, et al. 2020. Cancer Sci. . PubMed
  162. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  163. Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed
  164. Jadhav U, et al. 2019. Mol Cell. 74:542. PubMed
  165. Renz BW, et al. 2018. Cancer Cell. 33:75. PubMed
  166. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
  167. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  168. Chen Z, et al. 2020. Immunity. 54(1):151-163.e6. PubMed
RRID
AB_1134105 (BioLegend Cat. No. 118213)
AB_1134102 (BioLegend Cat. No. 118214)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD326 (EpCAM) (Maxpar® Ready) G8.8 FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (EpCAM) G8.8 FC
Brilliant Violet 510™ anti-mouse CD326 (EpCAM) G8.8 FC
Brilliant Violet 711™ anti-mouse CD326 (EpCAM) G8.8 FC
PE/Dazzle™ 594 anti-mouse CD326 (EpCAM) G8.8 FC
TotalSeq™-A0449 anti-mouse CD326 (EpCAM) G8.8 PG
Alexa Fluor® 700 anti-mouse CD326 (EpCAM) G8.8 FC
TotalSeq™-C0449 anti-mouse CD326 (EpCAM) G8.8 PG
TotalSeq™-B0449 anti-mouse CD326 (EpCAM) G8.8 PG
Brilliant Violet 650™ anti-mouse CD326 (EpCAM) G8.8 FC
Brilliant Violet 785™ anti-mouse CD326 (EpCAM) G8.8 FC
APC anti-mouse CD326 (EpCAM) G8.8 FC
PE anti-mouse CD326 (EpCAM) G8.8 FC
Alexa Fluor® 647 anti-mouse CD326 (EpCAM) G8.8 FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (EpCAM) G8.8 FC
Purified anti-mouse CD326 (EpCAM) G8.8 FC,IHC-F,ICC
Biotin anti-mouse CD326 (EpCAM) G8.8 FC
FITC anti-mouse CD326 (EpCAM) G8.8 FC
Alexa Fluor® 488 anti-mouse CD326 (EpCAM) G8.8 FC,IHC-F,3D IHC
APC/Cyanine7 anti-mouse CD326 (EpCAM) G8.8 FC
PerCP/Cyanine5.5 anti-mouse CD326 (EpCAM) G8.8 FC
Alexa Fluor® 594 anti-mouse CD326 (EpCAM) G8.8 ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (EpCAM) G8.8 FC
Brilliant Violet 605™ anti-mouse CD326 (EpCAM) G8.8 FC
PE/Cyanine5 anti-mouse CD326 (EpCAM) G8.8 FC
Spark Red™ 718 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 574 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 550 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark NIR™ 685 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark YG™ 593 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark YG™ 581 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
G8dot8_APC_081908.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
  • G8dot8_APC_081908.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
118213 25 µg DKK606
Check Availability


Need larger quantities of this item?
Request Bulk Quote
118214 100 µg DKK1759
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  2. Elkon R, et al. 2015. Nat Commun. 6: 8549. PubMed
  3. Chen Q, et al. 2020. Cell Reports. 31(13):107828. PubMed
  4. Park SE, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1235. PubMed
  5. Na E, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L550. PubMed
  6. Xu J, et al. 2022. Dev Cell. 57:839. PubMed
  7. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  8. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
  9. Cui W, et al. 2023. J Exp Med. 220:. PubMed
  10. Kalgudde Gopal S, et al. 2023. Nat Commun. 14:3020. PubMed
  11. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  12. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
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  22. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  23. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  24. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
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  42. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
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  49. Ionkina AA, et al. 2021. Breast Cancer Res. 23:93. PubMed
  50. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  51. Hertzano R, et al. 2020. Laryngoscope. . PubMed
  52. Li J, et al. 2020. Development. :. PubMed
  53. Cerretelli G, et al. 2021. J Pathol. 255:464. PubMed
  54. Tang Z, et al. 2021. Stem Cell Res Ther. 12:397. PubMed
  55. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  56. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  57. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  58. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  59. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  60. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  61. Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed
  62. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  63. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  64. Caporarello N, et al. 2020. Aging Cell. 19:e13196. PubMed
  65. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  66. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  67. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  68. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  69. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
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  71. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  72. Tanimizu N, et al. 2014. J Biol Chem. 289:7859. PubMed
  73. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  74. Casey O, et al. 2012. PLoS One. 7:e41668. PubMed
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  80. Jadhav U, et al. 2019. Mol Cell. 74:542. PubMed
  81. Renz BW, et al. 2018. Cancer Cell. 33:75. PubMed
  82. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
  83. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  84. Chen Z, et al. 2020. Immunity. 54(1):151-163.e6. PubMed
  85. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  86. Elkon R, et al. 2015. Nat Commun. 6: 8549. PubMed
  87. Chen Q, et al. 2020. Cell Reports. 31(13):107828. PubMed
  88. Park SE, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1235. PubMed
  89. Na E, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L550. PubMed
  90. Xu J, et al. 2022. Dev Cell. 57:839. PubMed
  91. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  92. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
  93. Cui W, et al. 2023. J Exp Med. 220:. PubMed
  94. Kalgudde Gopal S, et al. 2023. Nat Commun. 14:3020. PubMed
  95. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  96. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
  97. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  98. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  99. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  100. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  101. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  102. Mevel R, et al. 2020. eLife. 9:00. PubMed
  103. Buerger S, et al. 2015. J Immunol. 195: 4106 - 4116. PubMed
  104. Malhotra D, et al. 2016. Nat Immunol. 17:187-195. PubMed
  105. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
  106. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  107. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  108. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  109. Ford MJ, et al. 2021. Cell Rep. 36:109677. PubMed
  110. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  111. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  112. Phung HT, et al. 2020. Immunohorizons. 0.25625. PubMed
  113. Chua CW, et al. 2018. Elife. 7. PubMed
  114. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  115. Semba T, et al. 2020. Cancers (Basel). 12:. PubMed
  116. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
  117. Willobee BA, et al. 2021. Mol Cancer Ther. 20:1246. PubMed
  118. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  119. Wang Y, et al. 2014. PLoS One. 9:113127. PubMed
  120. Katara GK, et al. 2020. Mol Oncol. 0.729861111. PubMed
  121. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  122. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  123. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  124. Rudnick JA, et al. 2021. Genes Dev. 35:963. PubMed
  125. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  126. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  127. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  128. Bar C, et al. 2020. Cell Reports. 28(1):257-266.e5.. PubMed
  129. Jiang M, et al. 2021. Developmental Cell. 56(11):1646-1660.e5. PubMed
  130. Sateriale A, et al. 2020. Cell Host & Microbe. 26(1):135-146.e5.. PubMed
  131. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  132. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  133. Ionkina AA, et al. 2021. Breast Cancer Res. 23:93. PubMed
  134. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  135. Hertzano R, et al. 2020. Laryngoscope. . PubMed
  136. Li J, et al. 2020. Development. :. PubMed
  137. Cerretelli G, et al. 2021. J Pathol. 255:464. PubMed
  138. Tang Z, et al. 2021. Stem Cell Res Ther. 12:397. PubMed
  139. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  140. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  141. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  142. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  143. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  144. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  145. Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed
  146. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  147. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  148. Caporarello N, et al. 2020. Aging Cell. 19:e13196. PubMed
  149. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  150. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  151. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  152. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  153. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  154. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  155. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  156. Tanimizu N, et al. 2014. J Biol Chem. 289:7859. PubMed
  157. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  158. Casey O, et al. 2012. PLoS One. 7:e41668. PubMed
  159. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  160. Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed
  161. Kasuga A, et al. 2020. Cancer Sci. . PubMed
  162. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  163. Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed
  164. Jadhav U, et al. 2019. Mol Cell. 74:542. PubMed
  165. Renz BW, et al. 2018. Cancer Cell. 33:75. PubMed
  166. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
  167. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  168. Chen Z, et al. 2020. Immunity. 54(1):151-163.e6. PubMed
RRID
AB_1134105 (BioLegend Cat. No. 118213)
AB_1134102 (BioLegend Cat. No. 118214)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD326 (EpCAM) (Maxpar® Ready) G8.8 FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (EpCAM) G8.8 FC
Brilliant Violet 510™ anti-mouse CD326 (EpCAM) G8.8 FC
Brilliant Violet 711™ anti-mouse CD326 (EpCAM) G8.8 FC
PE/Dazzle™ 594 anti-mouse CD326 (EpCAM) G8.8 FC
TotalSeq™-A0449 anti-mouse CD326 (EpCAM) G8.8 PG
Alexa Fluor® 700 anti-mouse CD326 (EpCAM) G8.8 FC
TotalSeq™-C0449 anti-mouse CD326 (EpCAM) G8.8 PG
TotalSeq™-B0449 anti-mouse CD326 (EpCAM) G8.8 PG
Brilliant Violet 650™ anti-mouse CD326 (EpCAM) G8.8 FC
Brilliant Violet 785™ anti-mouse CD326 (EpCAM) G8.8 FC
APC anti-mouse CD326 (EpCAM) G8.8 FC
PE anti-mouse CD326 (EpCAM) G8.8 FC
Alexa Fluor® 647 anti-mouse CD326 (EpCAM) G8.8 FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (EpCAM) G8.8 FC
Purified anti-mouse CD326 (EpCAM) G8.8 FC,IHC-F,ICC
Biotin anti-mouse CD326 (EpCAM) G8.8 FC
FITC anti-mouse CD326 (EpCAM) G8.8 FC
Alexa Fluor® 488 anti-mouse CD326 (EpCAM) G8.8 FC,IHC-F,3D IHC
APC/Cyanine7 anti-mouse CD326 (EpCAM) G8.8 FC
PerCP/Cyanine5.5 anti-mouse CD326 (EpCAM) G8.8 FC
Alexa Fluor® 594 anti-mouse CD326 (EpCAM) G8.8 ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (EpCAM) G8.8 FC
Brilliant Violet 605™ anti-mouse CD326 (EpCAM) G8.8 FC
PE/Cyanine5 anti-mouse CD326 (EpCAM) G8.8 FC
Spark Red™ 718 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 574 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 550 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark NIR™ 685 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark YG™ 593 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Spark YG™ 581 anti-mouse CD326 (EpCAM) (Flexi-Fluor™) G8.8 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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