Loading...

Pacific Blue™ anti-mouse CD45 Antibody Pacific Blue™ anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-F11_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 30-F11
  • 30-F11_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 30-F11
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
103125 25 µg DKK559
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103126 100 µg DKK1278
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Aguilar EG, et al. 2021. Blood Adv. 5:4219. PubMed
  2. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  3. Quintero H, et al. 2022. Cell Rep. 40:111324. PubMed
  4. Grünewald M, et al. 2021. Science. 373: . PubMed
  5. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  6. Heitzeneder S, et al. 2022. Cancer Cell. 40:53. PubMed
  7. Meng J, et al. 2022. Cell Rep. 38:110492. PubMed
  8. Roy-Luzarraga M, et al. 2022. Cancer Res. 82:1909. PubMed
  9. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  10. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  11. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  12. Tran TA, et al. 2022. Front Immunol. 13:1007285. PubMed
  13. Kim JW, et al. 2022. iScience. 25:105603. PubMed
  14. Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed
  15. Vedunova MV, et al. 2022. Cell Death Dis. 13:1062. PubMed
  16. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  17. Berube A, et al. 2022. Viruses. 14:. PubMed
  18. Perveen S, et al. 2012. PLoS One. 7:e51945. PubMed
  19. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  20. Barkaway A, et al. 2021. Immunity. . PubMed
  21. Girbl T et al. 2018. Immunity. 49(6):1062-1076 . PubMed
  22. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  23. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  24. Ma L, et al. 2009. J Immunol. 183:1044. PubMed
  25. Svec J, et al. 2022. Cancers (Basel). 14:. PubMed
  26. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  27. Glorieux C, et al. 2022. J Adv Res. 40:109. PubMed
  28. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  29. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  30. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  31. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  32. Sakae H, et al. 2023. Cells. 12:. PubMed
  33. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  34. Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed
  35. Fukuzaki Y, et al. 2023. iScience. 26:106340. PubMed
  36. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  37. Wang Z, et al. 2023. J Cancer. 14:1049. PubMed
  38. Hu Y, et al. 2023. JCI Insight. 8:. PubMed
  39. Li H, et al. 2022. Cell Rep Med. 3:100554. PubMed
  40. Reinitz F, et al. 2022. Elife. 11:. PubMed
  41. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  42. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  43. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  44. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  45. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  46. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  47. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
  48. Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed
  49. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  50. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  51. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  52. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  53. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  54. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  55. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  56. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  57. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  58. Deslyper G, et al. 2021. Parasitology. 148:539. PubMed
  59. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  60. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  61. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  62. Stahnke S, et al. 2021. Current Biology. 31(10):2051-2064.e8. PubMed
  63. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  64. Peuhu E, et al. 2017. PLoS One. 12:e0186628. PubMed
  65. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  66. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  67. Chan A, et al. 2014. Am J Pathol. 184:1411. PubMed
  68. Scheele C, et al. 2017. Nature. 542:313-317. PubMed
  69. Okamoto T, et al. 2020. Cancer Res. 3580:80. PubMed
  70. Minatoguchi S, et al. 2022. Sci Rep. 12:5389. PubMed
  71. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  72. Guo HF, et al. 2021. Commun Biol. 4:482. PubMed
  73. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  74. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  75. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  76. Nie M, et al. 2016. Cell Death Dis. 7:e2261. PubMed
  77. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  78. Lee C, et al. 2014. Cancer Res. 74:341. PubMed
  79. Shi C, et al. 2010. J Immunol. 184:6266. PubMed
  80. An J, et al. 2022. iScience. 25:103570. PubMed
  81. Jeong SJ, et al. 2021. Autophagy. 17:3740. PubMed
  82. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  83. Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed
  84. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  85. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  86. Mifflin L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  87. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  88. Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
  89. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  90. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  91. Rosen D, et al. 2016. J Infect Dis. 213: 649 - 658. PubMed
  92. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  93. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  94. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  95. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  96. Bignami F, et al. 2017. Acta Ophthalmol. 95:e641. PubMed
  97. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  98. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  99. Cheah M, et al. 2015. Proc Natl Acad Sci U S A. 112:4725. PubMed
  100. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  101. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  102. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  103. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  104. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  105. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
  106. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  107. McCoy S 2015. Am J Physiol Lung Cell Mol Physiol. 309: L463-L474. PubMed
  108. Frasca D, et al. 2020. Front Immunol. 11:328. PubMed
  109. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  110. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  111. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  112. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  113. Gusarova GA, et al. 2021. JCI Insight. 6: . PubMed
  114. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  115. Chang J, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2015121356. PubMed
  116. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  117. Diéguez–Hurtado R, et al. 2019. Nat Commun. 10:2817. PubMed
  118. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
  119. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  120. Mantilla-Rojas C, et al. 2022. Cancers (Basel). 14:. PubMed
  121. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  122. Sungur C, et al. 2013. Proc Natl Acad Sci U S A. 110:7401. PubMed
  123. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  124. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  125. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  126. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  127. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  128. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  129. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  130. Li Q, et al. 2022. Front Cardiovasc Med. 9:872102. PubMed
  131. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  132. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  133. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  134. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  135. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  136. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  137. Nishida C, et al. 2012. Blood. 119:5405. PubMed
  138. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
  139. Song J, et al. 2021. Am J Physiol Heart Circ Physiol. 320:H323. PubMed
  140. McAndrews KM, et al. 2021. J Biol Chem. 296:100523. PubMed
  141. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  142. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  143. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  144. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  145. Chia JSJ, et al. 2018. Nutrients. 1.313194444. PubMed
  146. Li Y et al. 2018. Cell Stem Cell. 23(2):181-192 . PubMed
  147. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  148. Engblom C, et al. 2017. Science. 358:6367. PubMed
  149. Li F, et al. 2022. Nat Commun. 13:4334. PubMed
  150. Choudhary S, et al. 2012. J Virol. 86:114. PubMed
  151. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  152. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  153. Li C, et al. 2018. Cell. 174:285. PubMed
  154. Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed
  155. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  156. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  157. Ko FC, et al. 2021. J Bone Miner Res. 36:1510. PubMed
  158. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  159. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  160. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  161. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  162. McKenzie MD, et al. 2019. Cell Stem Cell. 25:258. PubMed
  163. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  164. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  165. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  166. Tang S, et al. 2022. Virol J. 19:32. PubMed
  167. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  168. Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed
  169. Stefan KL, et al. 2020. Cell. 1312:183. PubMed
  170. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  171. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  172. Weidner T, et al. 2021. Nat Protoc. 16:3210. PubMed
  173. Yamaguchi T, et al. 2020. Eur J Immunol. 50:110. PubMed
  174. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  175. Fite BZ, et al. 2021. Sci Rep. 11:927. PubMed
  176. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  177. Sasai M, et al. 2021. J Exp Med. 218:. PubMed
  178. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  179. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  180. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  181. Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
  182. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
  183. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  184. Forese MG et al. 2019. Glia. 68(1):95-110 . PubMed
  185. Zilionis R et al. 2019. Immunity. 50(5):1317-1334 . PubMed
  186. Tucey TM et al. 2018. Cell metabolism. 27(5):988-1006 . PubMed
  187. Hu Y, et al. 2021. J Nanobiotechnology. 19:416. PubMed
  188. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  189. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  190. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  191. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  192. Hemanthakumar KA, et al. 2021. eLife. 10:00. PubMed
  193. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  194. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  195. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  196. Avey D et al. 2018. Cell reports. 24(13):3619-3629 . PubMed
  197. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  198. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  199. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  200. Shao S, et al. 2020. Proc Natl Acad Sci U S A. 117:8845. PubMed
  201. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  202. Cortez MA, et al. 2020. Nat Commun. 3.819444444. PubMed
  203. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  204. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  205. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  206. Jiang Q, et al. 2008. Blood. 112:2858. PubMed
  207. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  208. Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed
  209. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  210. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  211. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  212. Aguilar EG, et al. 2021. Blood Adv. 5:4219. PubMed
  213. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  214. Quintero H, et al. 2022. Cell Rep. 40:111324. PubMed
  215. Grünewald M, et al. 2021. Science. 373: . PubMed
  216. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  217. Heitzeneder S, et al. 2022. Cancer Cell. 40:53. PubMed
  218. Meng J, et al. 2022. Cell Rep. 38:110492. PubMed
  219. Roy-Luzarraga M, et al. 2022. Cancer Res. 82:1909. PubMed
  220. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  221. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  222. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  223. Tran TA, et al. 2022. Front Immunol. 13:1007285. PubMed
  224. Kim JW, et al. 2022. iScience. 25:105603. PubMed
  225. Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed
  226. Vedunova MV, et al. 2022. Cell Death Dis. 13:1062. PubMed
  227. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  228. Berube A, et al. 2022. Viruses. 14:. PubMed
  229. Perveen S, et al. 2012. PLoS One. 7:e51945. PubMed
  230. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  231. Barkaway A, et al. 2021. Immunity. . PubMed
  232. Girbl T et al. 2018. Immunity. 49(6):1062-1076 . PubMed
  233. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  234. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  235. Ma L, et al. 2009. J Immunol. 183:1044. PubMed
  236. Svec J, et al. 2022. Cancers (Basel). 14:. PubMed
  237. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  238. Glorieux C, et al. 2022. J Adv Res. 40:109. PubMed
  239. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  240. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  241. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  242. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  243. Sakae H, et al. 2023. Cells. 12:. PubMed
  244. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  245. Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed
  246. Fukuzaki Y, et al. 2023. iScience. 26:106340. PubMed
  247. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  248. Wang Z, et al. 2023. J Cancer. 14:1049. PubMed
  249. Hu Y, et al. 2023. JCI Insight. 8:. PubMed
  250. Li H, et al. 2022. Cell Rep Med. 3:100554. PubMed
  251. Reinitz F, et al. 2022. Elife. 11:. PubMed
  252. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  253. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  254. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  255. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  256. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  257. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  258. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
  259. Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed
  260. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  261. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  262. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  263. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  264. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  265. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  266. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  267. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  268. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  269. Deslyper G, et al. 2021. Parasitology. 148:539. PubMed
  270. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  271. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  272. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  273. Stahnke S, et al. 2021. Current Biology. 31(10):2051-2064.e8. PubMed
  274. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  275. Peuhu E, et al. 2017. PLoS One. 12:e0186628. PubMed
  276. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  277. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  278. Chan A, et al. 2014. Am J Pathol. 184:1411. PubMed
  279. Scheele C, et al. 2017. Nature. 542:313-317. PubMed
  280. Okamoto T, et al. 2020. Cancer Res. 3580:80. PubMed
  281. Minatoguchi S, et al. 2022. Sci Rep. 12:5389. PubMed
  282. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  283. Guo HF, et al. 2021. Commun Biol. 4:482. PubMed
  284. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  285. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  286. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  287. Nie M, et al. 2016. Cell Death Dis. 7:e2261. PubMed
  288. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  289. Lee C, et al. 2014. Cancer Res. 74:341. PubMed
  290. Shi C, et al. 2010. J Immunol. 184:6266. PubMed
  291. An J, et al. 2022. iScience. 25:103570. PubMed
  292. Jeong SJ, et al. 2021. Autophagy. 17:3740. PubMed
  293. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  294. Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed
  295. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  296. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  297. Mifflin L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  298. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  299. Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
  300. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  301. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  302. Rosen D, et al. 2016. J Infect Dis. 213: 649 - 658. PubMed
  303. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  304. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  305. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  306. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  307. Bignami F, et al. 2017. Acta Ophthalmol. 95:e641. PubMed
  308. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  309. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  310. Cheah M, et al. 2015. Proc Natl Acad Sci U S A. 112:4725. PubMed
  311. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  312. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  313. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  314. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  315. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  316. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
  317. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  318. McCoy S 2015. Am J Physiol Lung Cell Mol Physiol. 309: L463-L474. PubMed
  319. Frasca D, et al. 2020. Front Immunol. 11:328. PubMed
  320. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  321. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  322. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  323. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  324. Gusarova GA, et al. 2021. JCI Insight. 6: . PubMed
  325. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  326. Chang J, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2015121356. PubMed
  327. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  328. Diéguez–Hurtado R, et al. 2019. Nat Commun. 10:2817. PubMed
  329. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
  330. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  331. Mantilla-Rojas C, et al. 2022. Cancers (Basel). 14:. PubMed
  332. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  333. Sungur C, et al. 2013. Proc Natl Acad Sci U S A. 110:7401. PubMed
  334. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  335. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  336. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  337. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  338. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  339. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  340. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  341. Li Q, et al. 2022. Front Cardiovasc Med. 9:872102. PubMed
  342. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  343. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  344. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  345. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  346. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  347. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  348. Nishida C, et al. 2012. Blood. 119:5405. PubMed
  349. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
  350. Song J, et al. 2021. Am J Physiol Heart Circ Physiol. 320:H323. PubMed
  351. McAndrews KM, et al. 2021. J Biol Chem. 296:100523. PubMed
  352. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  353. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  354. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  355. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  356. Chia JSJ, et al. 2018. Nutrients. 1.313194444. PubMed
  357. Li Y et al. 2018. Cell Stem Cell. 23(2):181-192 . PubMed
  358. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  359. Engblom C, et al. 2017. Science. 358:6367. PubMed
  360. Li F, et al. 2022. Nat Commun. 13:4334. PubMed
  361. Choudhary S, et al. 2012. J Virol. 86:114. PubMed
  362. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  363. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  364. Li C, et al. 2018. Cell. 174:285. PubMed
  365. Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed
  366. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  367. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  368. Ko FC, et al. 2021. J Bone Miner Res. 36:1510. PubMed
  369. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  370. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  371. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  372. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  373. McKenzie MD, et al. 2019. Cell Stem Cell. 25:258. PubMed
  374. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  375. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  376. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  377. Tang S, et al. 2022. Virol J. 19:32. PubMed
  378. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  379. Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed
  380. Stefan KL, et al. 2020. Cell. 1312:183. PubMed
  381. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  382. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  383. Weidner T, et al. 2021. Nat Protoc. 16:3210. PubMed
  384. Yamaguchi T, et al. 2020. Eur J Immunol. 50:110. PubMed
  385. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  386. Fite BZ, et al. 2021. Sci Rep. 11:927. PubMed
  387. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  388. Sasai M, et al. 2021. J Exp Med. 218:. PubMed
  389. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  390. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  391. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  392. Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
  393. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
  394. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  395. Forese MG et al. 2019. Glia. 68(1):95-110 . PubMed
  396. Zilionis R et al. 2019. Immunity. 50(5):1317-1334 . PubMed
  397. Tucey TM et al. 2018. Cell metabolism. 27(5):988-1006 . PubMed
  398. Hu Y, et al. 2021. J Nanobiotechnology. 19:416. PubMed
  399. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  400. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  401. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  402. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  403. Hemanthakumar KA, et al. 2021. eLife. 10:00. PubMed
  404. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  405. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  406. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  407. Avey D et al. 2018. Cell reports. 24(13):3619-3629 . PubMed
  408. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  409. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  410. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  411. Shao S, et al. 2020. Proc Natl Acad Sci U S A. 117:8845. PubMed
  412. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  413. Cortez MA, et al. 2020. Nat Commun. 3.819444444. PubMed
  414. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  415. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  416. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  417. Jiang Q, et al. 2008. Blood. 112:2858. PubMed
  418. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  419. Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed
  420. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  421. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  422. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
RRID
AB_493536 (BioLegend Cat. No. 103125)
AB_493535 (BioLegend Cat. No. 103126)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine5 anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine7 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 30-F11 FC
Spark YG™ 581 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS V475™ anti-mouse CD45 Antibody 30-F11 FC
StarBright UltraViolet 740 anti-mouse CD45 30-F11 FC
Go To Top Version: 2    Revision Date: 05/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-F11_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 30-F11
  • 30-F11_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 30-F11
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
103125 25 µg DKK559
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103126 100 µg DKK1278
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Aguilar EG, et al. 2021. Blood Adv. 5:4219. PubMed
  2. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  3. Quintero H, et al. 2022. Cell Rep. 40:111324. PubMed
  4. Grünewald M, et al. 2021. Science. 373: . PubMed
  5. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  6. Heitzeneder S, et al. 2022. Cancer Cell. 40:53. PubMed
  7. Meng J, et al. 2022. Cell Rep. 38:110492. PubMed
  8. Roy-Luzarraga M, et al. 2022. Cancer Res. 82:1909. PubMed
  9. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  10. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  11. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  12. Tran TA, et al. 2022. Front Immunol. 13:1007285. PubMed
  13. Kim JW, et al. 2022. iScience. 25:105603. PubMed
  14. Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed
  15. Vedunova MV, et al. 2022. Cell Death Dis. 13:1062. PubMed
  16. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  17. Berube A, et al. 2022. Viruses. 14:. PubMed
  18. Perveen S, et al. 2012. PLoS One. 7:e51945. PubMed
  19. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  20. Barkaway A, et al. 2021. Immunity. . PubMed
  21. Girbl T et al. 2018. Immunity. 49(6):1062-1076 . PubMed
  22. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  23. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  24. Ma L, et al. 2009. J Immunol. 183:1044. PubMed
  25. Svec J, et al. 2022. Cancers (Basel). 14:. PubMed
  26. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  27. Glorieux C, et al. 2022. J Adv Res. 40:109. PubMed
  28. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  29. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  30. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  31. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  32. Sakae H, et al. 2023. Cells. 12:. PubMed
  33. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  34. Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed
  35. Fukuzaki Y, et al. 2023. iScience. 26:106340. PubMed
  36. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  37. Wang Z, et al. 2023. J Cancer. 14:1049. PubMed
  38. Hu Y, et al. 2023. JCI Insight. 8:. PubMed
  39. Li H, et al. 2022. Cell Rep Med. 3:100554. PubMed
  40. Reinitz F, et al. 2022. Elife. 11:. PubMed
  41. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  42. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  43. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  44. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  45. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  46. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  47. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
  48. Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed
  49. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  50. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  51. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  52. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  53. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  54. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  55. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  56. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  57. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  58. Deslyper G, et al. 2021. Parasitology. 148:539. PubMed
  59. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  60. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  61. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  62. Stahnke S, et al. 2021. Current Biology. 31(10):2051-2064.e8. PubMed
  63. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  64. Peuhu E, et al. 2017. PLoS One. 12:e0186628. PubMed
  65. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  66. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  67. Chan A, et al. 2014. Am J Pathol. 184:1411. PubMed
  68. Scheele C, et al. 2017. Nature. 542:313-317. PubMed
  69. Okamoto T, et al. 2020. Cancer Res. 3580:80. PubMed
  70. Minatoguchi S, et al. 2022. Sci Rep. 12:5389. PubMed
  71. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  72. Guo HF, et al. 2021. Commun Biol. 4:482. PubMed
  73. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  74. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  75. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  76. Nie M, et al. 2016. Cell Death Dis. 7:e2261. PubMed
  77. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  78. Lee C, et al. 2014. Cancer Res. 74:341. PubMed
  79. Shi C, et al. 2010. J Immunol. 184:6266. PubMed
  80. An J, et al. 2022. iScience. 25:103570. PubMed
  81. Jeong SJ, et al. 2021. Autophagy. 17:3740. PubMed
  82. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  83. Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed
  84. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  85. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  86. Mifflin L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  87. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  88. Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
  89. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  90. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  91. Rosen D, et al. 2016. J Infect Dis. 213: 649 - 658. PubMed
  92. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  93. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  94. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  95. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  96. Bignami F, et al. 2017. Acta Ophthalmol. 95:e641. PubMed
  97. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  98. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  99. Cheah M, et al. 2015. Proc Natl Acad Sci U S A. 112:4725. PubMed
  100. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  101. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  102. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  103. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  104. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  105. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
  106. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  107. McCoy S 2015. Am J Physiol Lung Cell Mol Physiol. 309: L463-L474. PubMed
  108. Frasca D, et al. 2020. Front Immunol. 11:328. PubMed
  109. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  110. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  111. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  112. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  113. Gusarova GA, et al. 2021. JCI Insight. 6: . PubMed
  114. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  115. Chang J, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2015121356. PubMed
  116. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  117. Diéguez–Hurtado R, et al. 2019. Nat Commun. 10:2817. PubMed
  118. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
  119. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  120. Mantilla-Rojas C, et al. 2022. Cancers (Basel). 14:. PubMed
  121. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  122. Sungur C, et al. 2013. Proc Natl Acad Sci U S A. 110:7401. PubMed
  123. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  124. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  125. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  126. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  127. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  128. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  129. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  130. Li Q, et al. 2022. Front Cardiovasc Med. 9:872102. PubMed
  131. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  132. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  133. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  134. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  135. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  136. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  137. Nishida C, et al. 2012. Blood. 119:5405. PubMed
  138. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
  139. Song J, et al. 2021. Am J Physiol Heart Circ Physiol. 320:H323. PubMed
  140. McAndrews KM, et al. 2021. J Biol Chem. 296:100523. PubMed
  141. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  142. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  143. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  144. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  145. Chia JSJ, et al. 2018. Nutrients. 1.313194444. PubMed
  146. Li Y et al. 2018. Cell Stem Cell. 23(2):181-192 . PubMed
  147. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  148. Engblom C, et al. 2017. Science. 358:6367. PubMed
  149. Li F, et al. 2022. Nat Commun. 13:4334. PubMed
  150. Choudhary S, et al. 2012. J Virol. 86:114. PubMed
  151. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  152. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  153. Li C, et al. 2018. Cell. 174:285. PubMed
  154. Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed
  155. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  156. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  157. Ko FC, et al. 2021. J Bone Miner Res. 36:1510. PubMed
  158. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  159. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  160. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  161. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  162. McKenzie MD, et al. 2019. Cell Stem Cell. 25:258. PubMed
  163. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  164. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  165. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  166. Tang S, et al. 2022. Virol J. 19:32. PubMed
  167. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  168. Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed
  169. Stefan KL, et al. 2020. Cell. 1312:183. PubMed
  170. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  171. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  172. Weidner T, et al. 2021. Nat Protoc. 16:3210. PubMed
  173. Yamaguchi T, et al. 2020. Eur J Immunol. 50:110. PubMed
  174. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  175. Fite BZ, et al. 2021. Sci Rep. 11:927. PubMed
  176. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  177. Sasai M, et al. 2021. J Exp Med. 218:. PubMed
  178. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  179. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  180. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  181. Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
  182. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
  183. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  184. Forese MG et al. 2019. Glia. 68(1):95-110 . PubMed
  185. Zilionis R et al. 2019. Immunity. 50(5):1317-1334 . PubMed
  186. Tucey TM et al. 2018. Cell metabolism. 27(5):988-1006 . PubMed
  187. Hu Y, et al. 2021. J Nanobiotechnology. 19:416. PubMed
  188. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  189. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  190. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  191. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  192. Hemanthakumar KA, et al. 2021. eLife. 10:00. PubMed
  193. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  194. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  195. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  196. Avey D et al. 2018. Cell reports. 24(13):3619-3629 . PubMed
  197. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  198. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  199. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  200. Shao S, et al. 2020. Proc Natl Acad Sci U S A. 117:8845. PubMed
  201. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  202. Cortez MA, et al. 2020. Nat Commun. 3.819444444. PubMed
  203. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  204. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  205. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  206. Jiang Q, et al. 2008. Blood. 112:2858. PubMed
  207. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  208. Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed
  209. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  210. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  211. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  212. Aguilar EG, et al. 2021. Blood Adv. 5:4219. PubMed
  213. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  214. Quintero H, et al. 2022. Cell Rep. 40:111324. PubMed
  215. Grünewald M, et al. 2021. Science. 373: . PubMed
  216. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  217. Heitzeneder S, et al. 2022. Cancer Cell. 40:53. PubMed
  218. Meng J, et al. 2022. Cell Rep. 38:110492. PubMed
  219. Roy-Luzarraga M, et al. 2022. Cancer Res. 82:1909. PubMed
  220. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  221. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  222. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  223. Tran TA, et al. 2022. Front Immunol. 13:1007285. PubMed
  224. Kim JW, et al. 2022. iScience. 25:105603. PubMed
  225. Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed
  226. Vedunova MV, et al. 2022. Cell Death Dis. 13:1062. PubMed
  227. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  228. Berube A, et al. 2022. Viruses. 14:. PubMed
  229. Perveen S, et al. 2012. PLoS One. 7:e51945. PubMed
  230. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  231. Barkaway A, et al. 2021. Immunity. . PubMed
  232. Girbl T et al. 2018. Immunity. 49(6):1062-1076 . PubMed
  233. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  234. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  235. Ma L, et al. 2009. J Immunol. 183:1044. PubMed
  236. Svec J, et al. 2022. Cancers (Basel). 14:. PubMed
  237. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  238. Glorieux C, et al. 2022. J Adv Res. 40:109. PubMed
  239. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  240. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  241. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  242. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  243. Sakae H, et al. 2023. Cells. 12:. PubMed
  244. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  245. Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed
  246. Fukuzaki Y, et al. 2023. iScience. 26:106340. PubMed
  247. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  248. Wang Z, et al. 2023. J Cancer. 14:1049. PubMed
  249. Hu Y, et al. 2023. JCI Insight. 8:. PubMed
  250. Li H, et al. 2022. Cell Rep Med. 3:100554. PubMed
  251. Reinitz F, et al. 2022. Elife. 11:. PubMed
  252. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  253. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  254. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  255. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  256. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  257. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  258. Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed
  259. Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed
  260. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  261. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  262. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  263. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  264. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  265. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  266. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  267. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  268. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  269. Deslyper G, et al. 2021. Parasitology. 148:539. PubMed
  270. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  271. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  272. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  273. Stahnke S, et al. 2021. Current Biology. 31(10):2051-2064.e8. PubMed
  274. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  275. Peuhu E, et al. 2017. PLoS One. 12:e0186628. PubMed
  276. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  277. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  278. Chan A, et al. 2014. Am J Pathol. 184:1411. PubMed
  279. Scheele C, et al. 2017. Nature. 542:313-317. PubMed
  280. Okamoto T, et al. 2020. Cancer Res. 3580:80. PubMed
  281. Minatoguchi S, et al. 2022. Sci Rep. 12:5389. PubMed
  282. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  283. Guo HF, et al. 2021. Commun Biol. 4:482. PubMed
  284. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  285. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  286. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  287. Nie M, et al. 2016. Cell Death Dis. 7:e2261. PubMed
  288. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  289. Lee C, et al. 2014. Cancer Res. 74:341. PubMed
  290. Shi C, et al. 2010. J Immunol. 184:6266. PubMed
  291. An J, et al. 2022. iScience. 25:103570. PubMed
  292. Jeong SJ, et al. 2021. Autophagy. 17:3740. PubMed
  293. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  294. Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed
  295. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  296. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  297. Mifflin L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  298. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  299. Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
  300. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
  301. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  302. Rosen D, et al. 2016. J Infect Dis. 213: 649 - 658. PubMed
  303. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  304. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  305. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  306. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  307. Bignami F, et al. 2017. Acta Ophthalmol. 95:e641. PubMed
  308. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  309. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  310. Cheah M, et al. 2015. Proc Natl Acad Sci U S A. 112:4725. PubMed
  311. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  312. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  313. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  314. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  315. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  316. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
  317. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  318. McCoy S 2015. Am J Physiol Lung Cell Mol Physiol. 309: L463-L474. PubMed
  319. Frasca D, et al. 2020. Front Immunol. 11:328. PubMed
  320. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  321. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  322. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  323. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  324. Gusarova GA, et al. 2021. JCI Insight. 6: . PubMed
  325. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  326. Chang J, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2015121356. PubMed
  327. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  328. Diéguez–Hurtado R, et al. 2019. Nat Commun. 10:2817. PubMed
  329. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
  330. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  331. Mantilla-Rojas C, et al. 2022. Cancers (Basel). 14:. PubMed
  332. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  333. Sungur C, et al. 2013. Proc Natl Acad Sci U S A. 110:7401. PubMed
  334. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  335. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  336. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  337. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  338. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  339. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  340. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  341. Li Q, et al. 2022. Front Cardiovasc Med. 9:872102. PubMed
  342. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  343. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  344. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  345. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  346. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  347. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  348. Nishida C, et al. 2012. Blood. 119:5405. PubMed
  349. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
  350. Song J, et al. 2021. Am J Physiol Heart Circ Physiol. 320:H323. PubMed
  351. McAndrews KM, et al. 2021. J Biol Chem. 296:100523. PubMed
  352. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  353. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  354. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  355. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  356. Chia JSJ, et al. 2018. Nutrients. 1.313194444. PubMed
  357. Li Y et al. 2018. Cell Stem Cell. 23(2):181-192 . PubMed
  358. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  359. Engblom C, et al. 2017. Science. 358:6367. PubMed
  360. Li F, et al. 2022. Nat Commun. 13:4334. PubMed
  361. Choudhary S, et al. 2012. J Virol. 86:114. PubMed
  362. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  363. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  364. Li C, et al. 2018. Cell. 174:285. PubMed
  365. Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed
  366. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  367. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  368. Ko FC, et al. 2021. J Bone Miner Res. 36:1510. PubMed
  369. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  370. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  371. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  372. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  373. McKenzie MD, et al. 2019. Cell Stem Cell. 25:258. PubMed
  374. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  375. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  376. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  377. Tang S, et al. 2022. Virol J. 19:32. PubMed
  378. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  379. Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed
  380. Stefan KL, et al. 2020. Cell. 1312:183. PubMed
  381. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  382. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  383. Weidner T, et al. 2021. Nat Protoc. 16:3210. PubMed
  384. Yamaguchi T, et al. 2020. Eur J Immunol. 50:110. PubMed
  385. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  386. Fite BZ, et al. 2021. Sci Rep. 11:927. PubMed
  387. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  388. Sasai M, et al. 2021. J Exp Med. 218:. PubMed
  389. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  390. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  391. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  392. Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
  393. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
  394. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  395. Forese MG et al. 2019. Glia. 68(1):95-110 . PubMed
  396. Zilionis R et al. 2019. Immunity. 50(5):1317-1334 . PubMed
  397. Tucey TM et al. 2018. Cell metabolism. 27(5):988-1006 . PubMed
  398. Hu Y, et al. 2021. J Nanobiotechnology. 19:416. PubMed
  399. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  400. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  401. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  402. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  403. Hemanthakumar KA, et al. 2021. eLife. 10:00. PubMed
  404. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  405. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  406. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  407. Avey D et al. 2018. Cell reports. 24(13):3619-3629 . PubMed
  408. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  409. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  410. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  411. Shao S, et al. 2020. Proc Natl Acad Sci U S A. 117:8845. PubMed
  412. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  413. Cortez MA, et al. 2020. Nat Commun. 3.819444444. PubMed
  414. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  415. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  416. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  417. Jiang Q, et al. 2008. Blood. 112:2858. PubMed
  418. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  419. Barry DM, et al. 2019. Nat Commun. 4.378472222. PubMed
  420. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  421. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  422. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
RRID
AB_493536 (BioLegend Cat. No. 103125)
AB_493535 (BioLegend Cat. No. 103126)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine5 anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine7 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 30-F11 FC
Spark YG™ 581 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS V475™ anti-mouse CD45 Antibody 30-F11 FC
StarBright UltraViolet 740 anti-mouse CD45 30-F11 FC
Go To Top Version: 2    Revision Date: 05/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account