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PE anti-mouse CD150 (SLAM) Antibody PE anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
TC15-12F12.2
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12.2
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12_PE_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE (filled histogram) or rat IgG2a PE isotype control (open histogram) (gated on lymphoid cell population).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
115903 25 µg DKK618
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115904 100 µg DKK1592
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  2. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  3. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  4. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  5. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  6. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  7. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  8. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  9. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  10. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  11. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  12. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  13. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  14. Hu W, et al. 2015. Blood. 125:2206. PubMed
  15. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  16. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  17. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  18. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  19. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  20. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  21. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  22. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  23. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  24. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  25. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  26. Gao B, et al. 2018. Aging Cell. 3. PubMed
  27. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  28. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  29. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  30. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  31. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  32. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  33. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  34. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  35. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  36. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  37. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  38. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  39. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  40. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  41. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  42. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  43. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  44. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  45. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  46. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  47. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  48. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  49. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  50. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  51. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  52. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  53. Ise W, et al. 2018. Immunity. 48:702. PubMed
  54. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  55. Kim I, et al. 2006. Blood. 108:737. PubMed
  56. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  57. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  58. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  59. Dong S, et al. 2021. Nature. 591:117. PubMed
  60. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  61. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  62. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  63. Jung Y, et al. 2007. Blood . 110:82. PubMed
  64. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  65. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  66. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  67. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  68. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  69. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  70. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  71. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  72. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  73. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  74. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  75. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  76. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  77. Song J, et al. 2010. Blood. 115:2592. PubMed
  78. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  79. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  80. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  81. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  82. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  83. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  84. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  85. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  86. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  87. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  88. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  89. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  90. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  91. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  92. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  93. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  94. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  95. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  96. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  97. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  98. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  99. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  100. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  101. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  102. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  103. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  104. Gao X, et al. 2021. Nature. 589:591. PubMed
  105. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  106. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  107. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  108. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  109. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  110. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  111. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  112. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  113. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  114. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  115. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  116. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  117. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  118. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  119. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  120. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  121. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  122. Fraser S, et al. 2007. Blood. 109:343. PubMed
  123. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  124. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  125. Xie M, et al. 2020. Haematologica. . PubMed
  126. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  127. Man N, et al. 2021. JCI Insight. 6:. PubMed
  128. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  129. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  130. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  131. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  132. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  133. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  134. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  135. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  136. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  137. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  138. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  139. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  140. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  141. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  142. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  143. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  144. Hu W, et al. 2015. Blood. 125:2206. PubMed
  145. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  146. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  147. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  148. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  149. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  150. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  151. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  152. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  153. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  154. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  155. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  156. Gao B, et al. 2018. Aging Cell. 3. PubMed
  157. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  158. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  159. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  160. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  161. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  162. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  163. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  164. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  165. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  166. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  167. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  168. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  169. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  170. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  171. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  172. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  173. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  174. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  175. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  176. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  177. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  178. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  179. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  180. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  181. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  182. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  183. Ise W, et al. 2018. Immunity. 48:702. PubMed
  184. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  185. Kim I, et al. 2006. Blood. 108:737. PubMed
  186. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  187. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  188. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  189. Dong S, et al. 2021. Nature. 591:117. PubMed
  190. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  191. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  192. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  193. Jung Y, et al. 2007. Blood . 110:82. PubMed
  194. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  195. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  196. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  197. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  198. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  199. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  200. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  201. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  202. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  203. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  204. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  205. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  206. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  207. Song J, et al. 2010. Blood. 115:2592. PubMed
  208. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  209. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  210. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  211. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  212. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  213. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  214. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  215. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  216. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  217. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  218. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  219. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  220. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  221. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  222. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  223. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  224. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  225. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  226. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  227. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  228. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  229. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  230. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  231. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  232. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  233. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  234. Gao X, et al. 2021. Nature. 589:591. PubMed
  235. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  236. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  237. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  238. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  239. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  240. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  241. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  242. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  243. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  244. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  245. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  246. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  247. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  248. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  249. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  250. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  251. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  252. Fraser S, et al. 2007. Blood. 109:343. PubMed
  253. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  254. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  255. Xie M, et al. 2020. Haematologica. . PubMed
  256. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  257. Man N, et al. 2021. JCI Insight. 6:. PubMed
  258. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  259. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  260. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
RRID
AB_313682 (BioLegend Cat. No. 115903)
AB_313683 (BioLegend Cat. No. 115904)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
TC15-12F12.2
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12.2
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12_PE_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE (filled histogram) or rat IgG2a PE isotype control (open histogram) (gated on lymphoid cell population).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
115903 25 µg DKK618
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115904 100 µg DKK1592
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  2. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  3. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  4. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  5. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  6. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  7. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  8. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  9. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  10. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  11. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  12. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  13. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  14. Hu W, et al. 2015. Blood. 125:2206. PubMed
  15. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  16. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  17. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  18. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  19. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  20. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  21. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  22. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  23. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  24. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  25. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  26. Gao B, et al. 2018. Aging Cell. 3. PubMed
  27. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  28. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  29. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  30. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  31. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  32. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  33. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  34. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  35. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  36. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  37. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  38. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  39. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  40. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  41. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  42. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  43. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  44. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  45. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  46. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  47. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  48. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  49. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  50. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  51. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  52. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  53. Ise W, et al. 2018. Immunity. 48:702. PubMed
  54. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  55. Kim I, et al. 2006. Blood. 108:737. PubMed
  56. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  57. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  58. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  59. Dong S, et al. 2021. Nature. 591:117. PubMed
  60. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  61. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  62. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  63. Jung Y, et al. 2007. Blood . 110:82. PubMed
  64. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  65. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  66. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  67. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  68. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  69. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  70. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  71. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  72. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  73. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  74. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  75. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  76. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  77. Song J, et al. 2010. Blood. 115:2592. PubMed
  78. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  79. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  80. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  81. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  82. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  83. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  84. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  85. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  86. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  87. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  88. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  89. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  90. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  91. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  92. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  93. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  94. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  95. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  96. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  97. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  98. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  99. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  100. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  101. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  102. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  103. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  104. Gao X, et al. 2021. Nature. 589:591. PubMed
  105. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  106. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  107. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  108. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  109. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  110. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  111. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  112. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  113. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  114. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  115. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  116. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  117. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  118. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  119. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  120. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  121. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  122. Fraser S, et al. 2007. Blood. 109:343. PubMed
  123. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  124. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  125. Xie M, et al. 2020. Haematologica. . PubMed
  126. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  127. Man N, et al. 2021. JCI Insight. 6:. PubMed
  128. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  129. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  130. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  131. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  132. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  133. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  134. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  135. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  136. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  137. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  138. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  139. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  140. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  141. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  142. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  143. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  144. Hu W, et al. 2015. Blood. 125:2206. PubMed
  145. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  146. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  147. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  148. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  149. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  150. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  151. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  152. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  153. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  154. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  155. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  156. Gao B, et al. 2018. Aging Cell. 3. PubMed
  157. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  158. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  159. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  160. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  161. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  162. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  163. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  164. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  165. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  166. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  167. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  168. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  169. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  170. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  171. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  172. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  173. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  174. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  175. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  176. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  177. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  178. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  179. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  180. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  181. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  182. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  183. Ise W, et al. 2018. Immunity. 48:702. PubMed
  184. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  185. Kim I, et al. 2006. Blood. 108:737. PubMed
  186. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  187. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  188. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  189. Dong S, et al. 2021. Nature. 591:117. PubMed
  190. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  191. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  192. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  193. Jung Y, et al. 2007. Blood . 110:82. PubMed
  194. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  195. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  196. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  197. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  198. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  199. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  200. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  201. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  202. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  203. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  204. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  205. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  206. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  207. Song J, et al. 2010. Blood. 115:2592. PubMed
  208. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  209. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  210. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  211. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  212. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  213. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  214. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  215. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  216. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  217. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  218. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  219. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  220. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  221. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  222. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  223. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  224. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  225. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  226. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  227. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  228. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  229. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  230. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  231. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  232. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  233. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  234. Gao X, et al. 2021. Nature. 589:591. PubMed
  235. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  236. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  237. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  238. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  239. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  240. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  241. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  242. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  243. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  244. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  245. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  246. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  247. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  248. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  249. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  250. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  251. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  252. Fraser S, et al. 2007. Blood. 109:343. PubMed
  253. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  254. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  255. Xie M, et al. 2020. Haematologica. . PubMed
  256. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  257. Man N, et al. 2021. JCI Insight. 6:. PubMed
  258. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
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  260. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
RRID
AB_313682 (BioLegend Cat. No. 115903)
AB_313683 (BioLegend Cat. No. 115904)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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