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PE anti-mouse CD150 (SLAM) Antibody PE anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
TC15-12F12.2
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12.2
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12_PE_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE (filled histogram) or rat IgG2a PE isotype control (open histogram) (gated on lymphoid cell population).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
115903 25 µg DKK618
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115904 100 µg DKK1592
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  2. Hu W, et al. 2015. Blood. 125:2206. PubMed
  3. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  4. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  5. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  6. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  7. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  8. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  9. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  10. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  11. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  12. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  13. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  14. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  15. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  16. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  17. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  18. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  19. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  20. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  21. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  22. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  23. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  24. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  25. Gao B, et al. 2018. Aging Cell. 3. PubMed
  26. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  27. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  28. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  29. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  30. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  31. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  32. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  33. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  34. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  35. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  36. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  37. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  38. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  39. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  40. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  41. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  42. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  43. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  44. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  45. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  46. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  47. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  48. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  49. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  50. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  51. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  52. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  53. Ise W, et al. 2018. Immunity. 48:702. PubMed
  54. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  55. Kim I, et al. 2006. Blood. 108:737. PubMed
  56. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  57. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  58. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  59. Dong S, et al. 2021. Nature. 591:117. PubMed
  60. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  61. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  62. Jung Y, et al. 2007. Blood . 110:82. PubMed
  63. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  64. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  65. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  66. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  67. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  68. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  69. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  70. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  71. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  72. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  73. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  74. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  75. Song J, et al. 2010. Blood. 115:2592. PubMed
  76. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  77. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  78. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  79. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  80. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  81. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  82. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  83. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  84. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  85. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  86. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  87. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  88. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  89. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  90. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  91. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  92. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  93. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  94. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  95. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  96. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  97. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  98. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  99. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  100. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  101. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  102. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  103. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  104. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  105. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  106. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  107. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  108. Gao X, et al. 2021. Nature. 589:591. PubMed
  109. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  110. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  111. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  112. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  113. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  114. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  115. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  116. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  117. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  118. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  119. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  120. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  121. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  122. Fraser S, et al. 2007. Blood. 109:343. PubMed
  123. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  124. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  125. Xie M, et al. 2020. Haematologica. . PubMed
  126. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  127. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  128. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  129. Man N, et al. 2021. JCI Insight. 6:. PubMed
  130. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  131. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  132. Hu W, et al. 2015. Blood. 125:2206. PubMed
  133. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  134. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  135. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  136. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  137. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  138. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  139. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  140. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  141. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  142. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  143. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  144. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  145. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  146. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  147. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  148. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  149. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  150. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  151. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  152. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  153. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  154. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  155. Gao B, et al. 2018. Aging Cell. 3. PubMed
  156. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  157. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  158. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  159. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  160. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  161. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  162. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  163. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  164. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  165. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  166. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  167. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  168. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  169. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  170. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  171. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  172. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  173. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  174. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  175. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  176. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  177. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  178. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  179. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  180. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  181. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  182. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  183. Ise W, et al. 2018. Immunity. 48:702. PubMed
  184. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  185. Kim I, et al. 2006. Blood. 108:737. PubMed
  186. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  187. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  188. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  189. Dong S, et al. 2021. Nature. 591:117. PubMed
  190. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  191. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  192. Jung Y, et al. 2007. Blood . 110:82. PubMed
  193. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  194. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  195. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  196. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  197. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  198. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  199. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  200. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  201. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  202. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  203. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  204. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  205. Song J, et al. 2010. Blood. 115:2592. PubMed
  206. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  207. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  208. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  209. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  210. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  211. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  212. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  213. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  214. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  215. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  216. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  217. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  218. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  219. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  220. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  221. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  222. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  223. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  224. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  225. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  226. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  227. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  228. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  229. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  230. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  231. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  232. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  233. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  234. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  235. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  236. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  237. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  238. Gao X, et al. 2021. Nature. 589:591. PubMed
  239. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  240. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  241. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  242. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  243. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  244. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  245. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  246. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  247. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  248. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  249. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  250. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  251. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  252. Fraser S, et al. 2007. Blood. 109:343. PubMed
  253. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  254. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  255. Xie M, et al. 2020. Haematologica. . PubMed
  256. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  257. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  258. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  259. Man N, et al. 2021. JCI Insight. 6:. PubMed
  260. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_313682 (BioLegend Cat. No. 115903)
AB_313683 (BioLegend Cat. No. 115904)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
TC15-12F12.2
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12.2
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12_PE_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE (filled histogram) or rat IgG2a PE isotype control (open histogram) (gated on lymphoid cell population).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
115903 25 µg DKK618
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115904 100 µg DKK1592
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  2. Hu W, et al. 2015. Blood. 125:2206. PubMed
  3. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  4. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  5. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  6. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  7. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  8. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  9. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  10. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  11. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  12. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  13. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  14. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  15. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  16. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  17. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  18. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  19. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  20. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  21. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  22. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  23. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  24. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  25. Gao B, et al. 2018. Aging Cell. 3. PubMed
  26. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  27. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  28. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  29. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  30. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  31. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  32. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  33. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  34. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  35. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  36. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  37. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  38. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  39. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  40. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  41. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  42. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  43. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  44. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  45. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  46. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  47. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  48. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  49. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  50. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  51. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  52. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  53. Ise W, et al. 2018. Immunity. 48:702. PubMed
  54. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  55. Kim I, et al. 2006. Blood. 108:737. PubMed
  56. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  57. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  58. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  59. Dong S, et al. 2021. Nature. 591:117. PubMed
  60. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  61. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  62. Jung Y, et al. 2007. Blood . 110:82. PubMed
  63. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  64. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  65. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  66. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  67. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  68. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  69. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  70. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  71. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  72. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  73. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  74. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  75. Song J, et al. 2010. Blood. 115:2592. PubMed
  76. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  77. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  78. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  79. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  80. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  81. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  82. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  83. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  84. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  85. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  86. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  87. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  88. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  89. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  90. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  91. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  92. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  93. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  94. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  95. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  96. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  97. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  98. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  99. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  100. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  101. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  102. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  103. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  104. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  105. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  106. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  107. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  108. Gao X, et al. 2021. Nature. 589:591. PubMed
  109. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  110. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  111. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  112. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  113. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  114. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  115. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  116. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  117. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  118. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  119. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  120. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  121. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  122. Fraser S, et al. 2007. Blood. 109:343. PubMed
  123. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  124. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  125. Xie M, et al. 2020. Haematologica. . PubMed
  126. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  127. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  128. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  129. Man N, et al. 2021. JCI Insight. 6:. PubMed
  130. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  131. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  132. Hu W, et al. 2015. Blood. 125:2206. PubMed
  133. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  134. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  135. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  136. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  137. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  138. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  139. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  140. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  141. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  142. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  143. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  144. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  145. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  146. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  147. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  148. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  149. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  150. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  151. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  152. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  153. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  154. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  155. Gao B, et al. 2018. Aging Cell. 3. PubMed
  156. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  157. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  158. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  159. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  160. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  161. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  162. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  163. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  164. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  165. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  166. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  167. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  168. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  169. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  170. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  171. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  172. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  173. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  174. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  175. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  176. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  177. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  178. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  179. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  180. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  181. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  182. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  183. Ise W, et al. 2018. Immunity. 48:702. PubMed
  184. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  185. Kim I, et al. 2006. Blood. 108:737. PubMed
  186. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  187. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  188. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  189. Dong S, et al. 2021. Nature. 591:117. PubMed
  190. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  191. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  192. Jung Y, et al. 2007. Blood . 110:82. PubMed
  193. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  194. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  195. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  196. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  197. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  198. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  199. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  200. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  201. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  202. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  203. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  204. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  205. Song J, et al. 2010. Blood. 115:2592. PubMed
  206. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  207. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  208. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  209. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  210. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  211. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  212. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  213. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  214. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  215. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  216. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  217. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  218. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  219. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  220. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  221. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  222. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  223. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  224. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  225. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  226. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  227. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  228. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  229. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  230. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  231. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  232. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  233. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  234. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  235. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  236. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  237. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  238. Gao X, et al. 2021. Nature. 589:591. PubMed
  239. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  240. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  241. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  242. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  243. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  244. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  245. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  246. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  247. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  248. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  249. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  250. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  251. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  252. Fraser S, et al. 2007. Blood. 109:343. PubMed
  253. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  254. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  255. Xie M, et al. 2020. Haematologica. . PubMed
  256. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  257. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  258. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  259. Man N, et al. 2021. JCI Insight. 6:. PubMed
  260. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_313682 (BioLegend Cat. No. 115903)
AB_313683 (BioLegend Cat. No. 115904)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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