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FITC anti-mouse Ly-6C Antibody FITC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_FITC_011409.jpg
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
  • HK1dot4_FITC_011409.jpg
    C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
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Cat # Size Price Quantity Check Availability Save
128006 500 µg NOK2418
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128005 50 µg NOK735
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
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  286. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  287. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  288. Hou PP, et al. 2020. Molecular Cell. 78(6):1192-1206.e10. PubMed
  289. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  290. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  291. Chiang N, et al. 2019. J Clin Invest. 129:5294. PubMed
  292. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  293. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  294. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  295. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  296. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  297. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  298. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  299. Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
  300. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  301. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  302. Meng Michelle Xu et al. 2017. Immunity. 47(2):363-373 . PubMed
  303. Caballero S, et al. 2017. Invest Ophthalmol Vis Sci. 58:5164. PubMed
  304. Tang S, et al. 2022. Virol J. 19:32. PubMed
  305. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  306. Donlan AN, et al. 2021. JCI Insight. 6: . PubMed
  307. Tyagi A, et al. 2021. Nat Commun. 12:474. PubMed
  308. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  309. Ngkelo A, et al. 2016. J Exp Med. 213: 1353 - 1374. PubMed
  310. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  311. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  312. Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed
  313. Asahara M, et al. 2022. PLoS One. 17:e0276135. PubMed
  314. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  315. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  316. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  317. Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed
  318. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  319. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  320. Rahtes A, et al. 2020. Front Immunol. 1.484722222. PubMed
  321. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  322. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  323. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  324. Jones GS, et al. 2020. mSphere. 5:. PubMed
  325. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  326. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  327. Reinke S, et al. 2020. Cell Reports. 30(8):2501-2511. PubMed
  328. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  329. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed
  330. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
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  332. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
RRID
AB_1186135 (BioLegend Cat. No. 128006)
AB_1186134 (BioLegend Cat. No. 128005)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Ly-6C Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse Ly-6C (Maxpar® Ready) HK1.4 FC,CyTOF®
Brilliant Violet 785™ anti-mouse Ly-6C HK1.4 FC
PE/Dazzle™ 594 anti-mouse Ly-6C HK1.4 FC
APC/Fire™ 750 anti-mouse Ly-6C HK1.4 FC
TotalSeq™-A0013 anti-mouse Ly-6C HK1.4 PG
Brilliant Violet 650™ anti-mouse Ly-6C HK1.4 FC
TotalSeq™-C0013 anti-mouse Ly-6C HK1.4 PG
TotalSeq™-B0013 anti-mouse Ly-6C HK1.4 PG
APC/Fire™ 810 anti-mouse Ly-6C Antibody HK1.4 FC
Spark Red™ 718 anti-mouse Ly-6C HK1.4 FC
Spark UV™ 387 anti-mouse Ly-6C HK1.4 FC
PE/Fire™ 810 anti-mouse Ly-6C HK1.4 FC
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™) HK1.4 FC
Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™) HK1.4 FC
PE/Cyanine5 anti-mouse Ly-6C HK1.4 FC
Spark PLUS B550™ anti-mouse Ly-6C HK1.4 FC
PerCP/Fire™ 806 anti-mouse Ly-6c Antibody HK1.4 FC
Spark YG™ 581 anti-mouse Ly-6C (Flexi-Fluor™) HK1.4 FC
Spark YG™ 593 anti-mouse Ly-6C (Flexi-Fluor™) Antibody HK1.4 FC
Spark NIR™ 685 anti-mouse Ly-6C (Flexi-Fluor™) Antibody HK1.4 FC
StarBright UltraViolet 740 anti-mouse Ly-6C HK1.4 FC
StarBright UltraViolet 795 anti-mouse Ly-6C HK1.4 FC
Spark PLUS UV395™ anti-mouse Ly-6C HK1.4 FC
Purified anti-mouse Ly-6C HK1.4 FC,IHC-F
Biotin anti-mouse Ly-6C HK1.4 FC
FITC anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 647 anti-mouse Ly-6C HK1.4 FC
PE anti-mouse Ly-6C HK1.4 FC
PerCP/Cyanine5.5 anti-mouse Ly-6C HK1.4 FC
Pacific Blue™ anti-mouse Ly-6C HK1.4 FC
APC anti-mouse Ly-6C HK1.4 FC
PE/Cyanine7 anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 488 anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 700 anti-mouse Ly-6C HK1.4 FC
APC/Cyanine7 anti-mouse Ly-6C HK1.4 FC
PerCP anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 570™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 421™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 510™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 605™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 711™ anti-mouse Ly-6C HK1.4 FC
Go To Top Version: 2    Revision Date: 12.09.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
Ave. Rating
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Product Citations
publications
HK1dot4_FITC_011409.jpg
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
  • HK1dot4_FITC_011409.jpg
    C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
128006 500 µg NOK2418
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128005 50 µg NOK735
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
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  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
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  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
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  168. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  169. de la Rosa X, et al. 2018. Am J Pathol. 188:950. PubMed
  170. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  171. Fang C, et al. 2022. J Pathol. 256:310. PubMed
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RRID
AB_1186135 (BioLegend Cat. No. 128006)
AB_1186134 (BioLegend Cat. No. 128005)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Other Formats

View All Ly-6C Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse Ly-6C (Maxpar® Ready) HK1.4 FC,CyTOF®
Brilliant Violet 785™ anti-mouse Ly-6C HK1.4 FC
PE/Dazzle™ 594 anti-mouse Ly-6C HK1.4 FC
APC/Fire™ 750 anti-mouse Ly-6C HK1.4 FC
TotalSeq™-A0013 anti-mouse Ly-6C HK1.4 PG
Brilliant Violet 650™ anti-mouse Ly-6C HK1.4 FC
TotalSeq™-C0013 anti-mouse Ly-6C HK1.4 PG
TotalSeq™-B0013 anti-mouse Ly-6C HK1.4 PG
APC/Fire™ 810 anti-mouse Ly-6C Antibody HK1.4 FC
Spark Red™ 718 anti-mouse Ly-6C HK1.4 FC
Spark UV™ 387 anti-mouse Ly-6C HK1.4 FC
PE/Fire™ 810 anti-mouse Ly-6C HK1.4 FC
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™) HK1.4 FC
Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™) HK1.4 FC
PE/Cyanine5 anti-mouse Ly-6C HK1.4 FC
Spark PLUS B550™ anti-mouse Ly-6C HK1.4 FC
PerCP/Fire™ 806 anti-mouse Ly-6c Antibody HK1.4 FC
Spark YG™ 581 anti-mouse Ly-6C (Flexi-Fluor™) HK1.4 FC
Spark YG™ 593 anti-mouse Ly-6C (Flexi-Fluor™) Antibody HK1.4 FC
Spark NIR™ 685 anti-mouse Ly-6C (Flexi-Fluor™) Antibody HK1.4 FC
StarBright UltraViolet 740 anti-mouse Ly-6C HK1.4 FC
StarBright UltraViolet 795 anti-mouse Ly-6C HK1.4 FC
Spark PLUS UV395™ anti-mouse Ly-6C HK1.4 FC
Purified anti-mouse Ly-6C HK1.4 FC,IHC-F
Biotin anti-mouse Ly-6C HK1.4 FC
FITC anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 647 anti-mouse Ly-6C HK1.4 FC
PE anti-mouse Ly-6C HK1.4 FC
PerCP/Cyanine5.5 anti-mouse Ly-6C HK1.4 FC
Pacific Blue™ anti-mouse Ly-6C HK1.4 FC
APC anti-mouse Ly-6C HK1.4 FC
PE/Cyanine7 anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 488 anti-mouse Ly-6C HK1.4 FC
Alexa Fluor® 700 anti-mouse Ly-6C HK1.4 FC
APC/Cyanine7 anti-mouse Ly-6C HK1.4 FC
PerCP anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 570™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 421™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 510™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 605™ anti-mouse Ly-6C HK1.4 FC
Brilliant Violet 711™ anti-mouse Ly-6C HK1.4 FC
Go To Top Version: 2    Revision Date: 12.09.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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