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APC/Cyanine7 anti-mouse I-A/I-E Antibody APC/Cyanine7 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
    C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
107628 100 µg SEK2453
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107627 25 µg SEK790
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  2. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  3. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  4. Ge W, et al. 2022. Evid Based Complement Alternat Med. 2022:6132289. PubMed
  5. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  6. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  7. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  8. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  9. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  10. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  11. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  12. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  13. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  14. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  15. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  16. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  17. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  18. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  19. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  20. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  21. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  22. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  23. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  24. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  25. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  26. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  27. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  28. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  29. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  30. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  31. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  32. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  33. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  34. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  35. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  36. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  37. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  38. Acker KP, et al. 2019. iScience. 19:281. PubMed
  39. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  40. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  41. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  42. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  43. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  44. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  45. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  46. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  47. França TT, et al. 2021. JCI Insight. 6:. PubMed
  48. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  49. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  50. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  51. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  52. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  53. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  54. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  55. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  56. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  57. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  58. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  59. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  60. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  61. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  62. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  63. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  64. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  65. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  66. Sung PS, et al. 2022. JCI Insight. :. PubMed
  67. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  68. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  69. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  70. Li J, et al. 2019. iScience. 16:468. PubMed
  71. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  72. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  73. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  74. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  75. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  76. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  77. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  78. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  79. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  80. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  81. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  82. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  83. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  84. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  85. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  86. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  87. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  88. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  89. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  90. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  91. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  92. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  93. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  94. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  95. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  96. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  97. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  98. Deets KA, et al. 2021. Elife. 10:. PubMed
  99. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  100. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  101. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  102. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
  103. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  104. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  105. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  106. Ge W, et al. 2022. Evid Based Complement Alternat Med. 2022:6132289. PubMed
  107. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  108. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  109. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  110. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  111. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  112. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  113. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  114. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  115. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  116. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  117. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  118. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  119. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  120. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  121. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  122. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  123. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  124. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  125. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  126. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  127. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  128. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  129. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  130. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  131. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  132. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  133. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  134. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  135. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  136. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  137. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  138. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  139. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  140. Acker KP, et al. 2019. iScience. 19:281. PubMed
  141. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  142. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  143. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  144. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  145. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  146. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  147. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  148. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  149. França TT, et al. 2021. JCI Insight. 6:. PubMed
  150. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  151. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  152. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  153. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  154. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  155. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  156. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  157. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  158. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  159. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  160. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  161. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  162. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  163. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  164. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  165. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  166. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  167. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  168. Sung PS, et al. 2022. JCI Insight. :. PubMed
  169. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  170. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  171. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  172. Li J, et al. 2019. iScience. 16:468. PubMed
  173. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  174. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  175. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  176. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  177. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  178. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  179. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  180. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  181. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  182. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  183. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  184. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  185. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  186. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  187. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  188. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  189. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  190. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  191. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  192. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  193. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  194. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  195. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  196. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  197. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  198. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  199. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  200. Deets KA, et al. 2021. Elife. 10:. PubMed
  201. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  202. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  203. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  204. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
RRID
AB_2069377 (BioLegend Cat. No. 107628)
AB_1659252 (BioLegend Cat. No. 107627)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 750™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
Spark NIR™ 685 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
StarBright UltraViolet 795 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS B574™ anti-mouse I-A/I-E Antibody M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 5    Revision Date: 02-01-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
    C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
107628 100 µg SEK2453
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107627 25 µg SEK790
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  2. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  3. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  4. Ge W, et al. 2022. Evid Based Complement Alternat Med. 2022:6132289. PubMed
  5. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  6. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  7. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  8. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  9. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  10. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  11. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  12. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  13. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  14. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  15. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  16. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  17. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  18. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  19. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  20. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  21. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  22. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  23. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  24. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  25. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  26. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  27. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  28. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  29. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  30. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  31. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  32. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  33. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  34. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  35. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  36. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  37. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  38. Acker KP, et al. 2019. iScience. 19:281. PubMed
  39. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  40. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  41. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  42. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  43. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  44. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  45. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  46. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  47. França TT, et al. 2021. JCI Insight. 6:. PubMed
  48. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  49. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  50. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  51. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  52. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  53. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  54. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  55. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  56. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  57. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  58. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  59. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  60. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  61. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  62. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  63. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  64. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  65. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  66. Sung PS, et al. 2022. JCI Insight. :. PubMed
  67. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  68. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  69. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  70. Li J, et al. 2019. iScience. 16:468. PubMed
  71. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  72. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  73. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  74. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  75. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  76. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  77. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  78. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  79. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  80. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  81. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  82. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  83. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  84. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  85. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  86. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  87. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  88. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  89. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  90. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  91. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  92. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  93. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  94. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  95. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  96. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  97. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  98. Deets KA, et al. 2021. Elife. 10:. PubMed
  99. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  100. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  101. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  102. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
  103. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  104. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  105. Atalis A, et al. 2022. J Control Release. 347:476. PubMed
  106. Ge W, et al. 2022. Evid Based Complement Alternat Med. 2022:6132289. PubMed
  107. Wang R, et al. 2023. Nat Biomed Eng. 7:38. PubMed
  108. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  109. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  110. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  111. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  112. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  113. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  114. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  115. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  116. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  117. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  118. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  119. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  120. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  121. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  122. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  123. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  124. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  125. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  126. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  127. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  128. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  129. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  130. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  131. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  132. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  133. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  134. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  135. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  136. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  137. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  138. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  139. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  140. Acker KP, et al. 2019. iScience. 19:281. PubMed
  141. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  142. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  143. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  144. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  145. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  146. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  147. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  148. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  149. França TT, et al. 2021. JCI Insight. 6:. PubMed
  150. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  151. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  152. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  153. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  154. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  155. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  156. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  157. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  158. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  159. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  160. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  161. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  162. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  163. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  164. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  165. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  166. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  167. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  168. Sung PS, et al. 2022. JCI Insight. :. PubMed
  169. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  170. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  171. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  172. Li J, et al. 2019. iScience. 16:468. PubMed
  173. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  174. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  175. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  176. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  177. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  178. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  179. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  180. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  181. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  182. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  183. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  184. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  185. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  186. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  187. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  188. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  189. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  190. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  191. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  192. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  193. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  194. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  195. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  196. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  197. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  198. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  199. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  200. Deets KA, et al. 2021. Elife. 10:. PubMed
  201. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  202. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  203. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  204. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
RRID
AB_2069377 (BioLegend Cat. No. 107628)
AB_1659252 (BioLegend Cat. No. 107627)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 750™ anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 593 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
Spark NIR™ 685 anti-mouse I-A/I-E (Flexi-Fluor™) Antibody M5/114.15.2 FC
StarBright UltraViolet 795 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS B574™ anti-mouse I-A/I-E Antibody M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 5    Revision Date: 02-01-2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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