C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
Application References
Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
Viville S, et al. 1993. Cell 72:635. (IHC)
Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
Yamashita I, et al. 1993. Int. Immunol. 5:1139.
Guo M, et al. 1995. Zygote 3:65. (IHC)
Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
Venanzi ES, et al. 2007. J. Immunol. 179:5693.
Christensen SR, et al. 2006. Immunity 25:417. PubMed
Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram).
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
Application References
Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
Viville S, et al. 1993. Cell 72:635. (IHC)
Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
Yamashita I, et al. 1993. Int. Immunol. 5:1139.
Guo M, et al. 1995. Zygote 3:65. (IHC)
Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
Venanzi ES, et al. 2007. J. Immunol. 179:5693.
Christensen SR, et al. 2006. Immunity 25:417. PubMed
Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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