Loading...

Biotin anti-mouse CD19 Antibody Biotin anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5_Biotin_080207
C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
  • 6D5_Biotin_080207
    C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
Compare all formats
Cat # Size Price Quantity Check Availability Save
115504 500 µg SEK1126
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115503 50 µg SEK454
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Song R, et al. 2022. Immunology. 165:274. PubMed
  2. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  3. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  4. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  5. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  6. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  7. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  8. Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed
  9. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  10. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  11. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  12. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  13. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  14. Xu H, et al. 2023. Elife. 12:. PubMed
  15. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
  16. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  17. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  18. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  19. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  20. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  21. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  22. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  23. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  24. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  25. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  26. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  27. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  28. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  29. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  30. Hoffmann A, et al. 2021. EBioMedicine. 71:103568. PubMed
  31. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  32. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  33. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  34. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  35. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  36. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  37. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  38. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  39. Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed
  40. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  41. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  42. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  43. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  44. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  45. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  46. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  47. Hutter K, et al. 2020. FEBS J. . PubMed
  48. Dong S, et al. 2021. Nature. 591:117. PubMed
  49. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  50. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  51. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  52. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  53. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  54. von Gamm M, et al. 2019. J Exp Med. 216:1700. PubMed
  55. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  56. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  57. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  58. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  59. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  60. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  61. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  62. Wolf SJ, et al. 2022. JCI Insight. 7:. PubMed
  63. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  64. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  65. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  66. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  67. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  68. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  69. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  70. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  71. Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed
  72. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  73. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  74. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  75. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  76. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  77. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  78. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  79. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  80. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  81. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  82. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
  83. Song R, et al. 2022. Immunology. 165:274. PubMed
  84. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  85. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  86. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  87. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  88. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  89. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  90. Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed
  91. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  92. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  93. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  94. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  95. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  96. Xu H, et al. 2023. Elife. 12:. PubMed
  97. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
  98. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  99. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  100. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  101. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  102. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  103. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  104. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  105. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  106. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  107. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  108. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  109. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  110. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  111. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  112. Hoffmann A, et al. 2021. EBioMedicine. 71:103568. PubMed
  113. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  114. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  115. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  116. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  117. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  118. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  119. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  120. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  121. Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed
  122. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  123. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  124. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  125. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  126. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  127. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  128. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  129. Hutter K, et al. 2020. FEBS J. . PubMed
  130. Dong S, et al. 2021. Nature. 591:117. PubMed
  131. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  132. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  133. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  134. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  135. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  136. von Gamm M, et al. 2019. J Exp Med. 216:1700. PubMed
  137. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  138. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  139. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  140. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  141. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  142. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  143. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  144. Wolf SJ, et al. 2022. JCI Insight. 7:. PubMed
  145. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  146. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  147. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  148. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  149. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  150. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  151. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  152. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  153. Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed
  154. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  155. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  156. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  157. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  158. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  159. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  160. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  161. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  162. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  163. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  164. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_313639 (BioLegend Cat. No. 115504)
AB_313638 (BioLegend Cat. No. 115503)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Spark PLUS UV395™ anti-mouse CD19 6D5 FC
Spark Violet™ 538 anti-mouse CD19 6D5 FC
PerCP/Fire™ 780 anti-mouse CD19 Antibody 6D5 FC
PerCP/Fire™ 806 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 593 anti-mouse CD19 (Flexi-Fluor™) Antibody 6D5 FC
Go To Top Version: 2    Revision Date: 05-24-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5_Biotin_080207
C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
  • 6D5_Biotin_080207
    C57BL/6 mouse splenocytes were stained with biotinylated CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by Sav-PE.
Compare all formats
Cat # Size Price Quantity Check Availability Save
115504 500 µg SEK1126
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115503 50 µg SEK454
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Song R, et al. 2022. Immunology. 165:274. PubMed
  2. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  3. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  4. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  5. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  6. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  7. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  8. Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed
  9. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  10. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  11. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  12. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  13. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  14. Xu H, et al. 2023. Elife. 12:. PubMed
  15. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
  16. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  17. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  18. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  19. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  20. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  21. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  22. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  23. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  24. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  25. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  26. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  27. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  28. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  29. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  30. Hoffmann A, et al. 2021. EBioMedicine. 71:103568. PubMed
  31. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  32. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  33. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  34. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  35. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  36. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  37. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  38. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  39. Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed
  40. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  41. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  42. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  43. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  44. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  45. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  46. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  47. Hutter K, et al. 2020. FEBS J. . PubMed
  48. Dong S, et al. 2021. Nature. 591:117. PubMed
  49. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  50. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  51. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  52. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  53. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  54. von Gamm M, et al. 2019. J Exp Med. 216:1700. PubMed
  55. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  56. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  57. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  58. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  59. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  60. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  61. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  62. Wolf SJ, et al. 2022. JCI Insight. 7:. PubMed
  63. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  64. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  65. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  66. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  67. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  68. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  69. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  70. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  71. Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed
  72. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  73. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  74. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  75. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  76. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  77. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  78. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  79. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  80. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  81. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  82. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
  83. Song R, et al. 2022. Immunology. 165:274. PubMed
  84. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  85. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  86. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  87. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  88. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  89. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  90. Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed
  91. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  92. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  93. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  94. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  95. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  96. Xu H, et al. 2023. Elife. 12:. PubMed
  97. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
  98. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  99. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  100. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  101. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  102. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  103. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  104. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  105. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  106. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  107. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  108. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  109. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  110. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  111. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  112. Hoffmann A, et al. 2021. EBioMedicine. 71:103568. PubMed
  113. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  114. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  115. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  116. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  117. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  118. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  119. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  120. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  121. Kunimoto H, et al. 2012. Sci Rep. 0.272916667. PubMed
  122. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  123. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  124. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  125. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  126. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  127. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  128. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  129. Hutter K, et al. 2020. FEBS J. . PubMed
  130. Dong S, et al. 2021. Nature. 591:117. PubMed
  131. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  132. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  133. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  134. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  135. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  136. von Gamm M, et al. 2019. J Exp Med. 216:1700. PubMed
  137. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  138. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  139. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  140. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  141. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  142. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  143. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  144. Wolf SJ, et al. 2022. JCI Insight. 7:. PubMed
  145. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  146. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  147. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  148. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  149. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  150. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  151. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  152. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  153. Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed
  154. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  155. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  156. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  157. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  158. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  159. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  160. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  161. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  162. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  163. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  164. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_313639 (BioLegend Cat. No. 115504)
AB_313638 (BioLegend Cat. No. 115503)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Spark PLUS UV395™ anti-mouse CD19 6D5 FC
Spark Violet™ 538 anti-mouse CD19 6D5 FC
PerCP/Fire™ 780 anti-mouse CD19 Antibody 6D5 FC
PerCP/Fire™ 806 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 593 anti-mouse CD19 (Flexi-Fluor™) Antibody 6D5 FC
Go To Top Version: 2    Revision Date: 05-24-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account