FITC anti-mouse CD19 Antibody FITC anti-mouse CD19 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CD19-expressing K562 human erythroleukemia cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁷.

Application References

1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
12. Olive V, et al. 2013. Elife. 2:822. PubMed
13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed

Product Citations

1. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
2. Collin R, et al. 2020. J Immunol. 205:133. PubMed
3. Li X, et al. 2022. J Clin Invest. 132: . PubMed
4. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
5. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
6. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
7. Hattori K, et al. 2023. Biomed Rep. 18:1. PubMed
8. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
9. Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed
10. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
11. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
12. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
13. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
14. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
15. Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed
16. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
17. Tsugawa H, et al. 2023. PLoS Pathog. 19:e1011139. PubMed
18. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
19. Gawish R, et al. 2022. Elife. 11:. PubMed
20. Chen CC, et al. 2021. J Exp Med. 218:. PubMed
21. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
22. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
23. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
24. Forte E, et al. 2020. J Cell Mol Med. . PubMed
25. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
26. Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed
27. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
28. Luff DH, et al. 2021. Front Immunol. 631271:12. PubMed
29. Das S, et al. 2019. Oncogene. 383316. PubMed
30. Antonia O Cuff et al. 2017. Wellcome open research. 2:39 . PubMed
31. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
32. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
33. Rothweiler S, et al. 2019. Purinergic Signal. 0.885416667. PubMed
34. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
35. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
36. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
37. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
38. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
39. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
40. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
41. Ma J, et al. 2016. Proc Natl Acad Sci U S A. 113: 450 - 458. PubMed
42. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
43. Omenetti S, et al. 2015. Cell Mol Gastroenterol Hepatol. 1: 406-419. PubMed
44. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
45. Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed
46. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
47. Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed
48. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
49. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
50. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
51. Sun S, et al. 2013. J Immunol. 190:6209. PubMed
52. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
53. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
54. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
55. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
56. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
57. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
58. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
59. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
60. Xu Y, et al. 2021. iScience. 24:103445. PubMed
61. Harrison DK, et al. 2021. Transplantation. 105:540. PubMed
62. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
63. Wang X, et al. 2022. Elife. 11:. PubMed
64. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
65. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
66. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
67. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
68. Gawish R, et al. 2022. Elife. 11:. PubMed
69. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
70. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
71. Zhang Y, et al. 2017. J Invest Dermatol. 137:2532. PubMed
72. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
73. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
74. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
75. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
76. Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed
77. Fan X, et al. 2014. PLoS One. 9:107638. PubMed
78. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
79. Collin R, et al. 2020. J Immunol. 205:133. PubMed
80. Li X, et al. 2022. J Clin Invest. 132: . PubMed
81. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
82. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
83. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
84. Hattori K, et al. 2023. Biomed Rep. 18:1. PubMed
85. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
86. Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed
87. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
88. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
89. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
90. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
91. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
92. Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed
93. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
94. Tsugawa H, et al. 2023. PLoS Pathog. 19:e1011139. PubMed
95. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
96. Gawish R, et al. 2022. Elife. 11:. PubMed
97. Chen CC, et al. 2021. J Exp Med. 218:. PubMed
98. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
99. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
100. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
101. Forte E, et al. 2020. J Cell Mol Med. . PubMed
102. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
103. Jia X, et al. 2021. Clin Transl Immunology. 10:e1336. PubMed
104. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
105. Luff DH, et al. 2021. Front Immunol. 631271:12. PubMed
106. Das S, et al. 2019. Oncogene. 383316. PubMed
107. Antonia O Cuff et al. 2017. Wellcome open research. 2:39 . PubMed
108. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
109. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
110. Rothweiler S, et al. 2019. Purinergic Signal. 0.885416667. PubMed
111. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
112. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
113. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
114. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
115. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
116. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
117. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
118. Ma J, et al. 2016. Proc Natl Acad Sci U S A. 113: 450 - 458. PubMed
119. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
120. Omenetti S, et al. 2015. Cell Mol Gastroenterol Hepatol. 1: 406-419. PubMed
121. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
122. Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed
123. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
124. Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed
125. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
126. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
127. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
128. Sun S, et al. 2013. J Immunol. 190:6209. PubMed
129. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
130. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
131. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
132. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
133. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
134. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
135. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
136. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
137. Xu Y, et al. 2021. iScience. 24:103445. PubMed
138. Harrison DK, et al. 2021. Transplantation. 105:540. PubMed
139. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
140. Wang X, et al. 2022. Elife. 11:. PubMed
141. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
142. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
143. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
144. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
145. Gawish R, et al. 2022. Elife. 11:. PubMed
146. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
147. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
148. Zhang Y, et al. 2017. J Invest Dermatol. 137:2532. PubMed
149. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
150. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
151. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
152. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
153. Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed
154. Fan X, et al. 2014. PLoS One. 9:107638. PubMed

RRID

AB_313641 (BioLegend Cat. No. 115506)
AB_313640 (BioLegend Cat. No. 115505)

Antigen Details

Structure

Ig superfamily, associates with CD21 and CD81, 95 kD

Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function

Modulates B cell activation and differentiation

Ligand/Receptor

CD21, CD81, Leu-13

Cell Type

B cells, Dendritic cells

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID

12478 View all products for this Gene ID

UniProt

View information about CD19 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis