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Spark UV™ 387 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) Antibody Spark UV™ 387 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) Antibody

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
Compare all formats See Spark UV™ 387 spectral data
Cat # Size Price Quantity Check Availability Save
285510 50 µg SEK2420
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark UV™ 387 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark UV™ 387 has a maximum excitation of 351 nm and a maximum emission of 387 nm.

Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB/199.

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Application References
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
  3. Taylor R, et al. 2007. J. Immunol. 178:5659.
  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer. 

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary. 

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL. 

 

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. 

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services. 

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance. 

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool. 

What if my requested antibody-dye combination doesn't perform as expected?

Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.

Other Formats

View All CD127 Reagents Request Custom Conjugation
Description Clone Applications
PE/Dazzle™ 594 anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 510™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Purified anti-mouse CD127 (IL-7Rα) (Maxpar® Ready) A7R34 FC,CyTOF®
Brilliant Violet 785™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 711™ anti-mouse CD127 (IL-7Rα) A7R34 FC
APC/Cyanine7 anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 650™ anti-mouse CD127 (IL-7Rα) A7R34 FC
TotalSeq™-A0198 anti-mouse CD127 (IL-7Rα) A7R34 PG
TotalSeq™-C0198 anti-mouse CD127 (IL-7Rα) A7R34 PG
Ultra-LEAF™ Purified anti-mouse CD127 (IL-7Rα) A7R34 FC,IP,WB,Block,IHC,ICC
TotalSeq™-B0198 anti-mouse CD127 (IL-7Rα) A7R34 PG
Purified anti-mouse CD127 (IL-7Rα) A7R34 FC,IP,WB,IHC,ICC
FITC anti-mouse CD127 (IL-7Rα) A7R34 FC
PE anti-mouse CD127 (IL-7Rα) A7R34 FC
APC anti-mouse CD127 (IL-7Rα) A7R34 FC
PE/Cyanine7 anti-mouse CD127 (IL-7Rα) A7R34 FC
PE/Cyanine5 anti-mouse CD127 (IL-7Rα) A7R34 FC
Alexa Fluor® 488 anti-mouse CD127 (IL-7Rα) A7R34 FC
Alexa Fluor® 647 anti-mouse CD127 (IL-7Rα) A7R34 FC
PerCP/Cyanine5.5 anti-mouse CD127 (IL-7Rα) A7R34 FC
Biotin anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 421™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 605™ anti-mouse CD127 (IL-7Rα) A7R34 FC
PerCP/Fire™ 780 anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 750™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Spark UV™ 387 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) A7R34 FC
Spark YG™ 581 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) A7R34 FC
Spark YG™ 593 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) A7R34 FC
Spark NIR™ 685 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) A7R34 FC
Spark PLUS B488™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Go To Top Version: 1    Revision Date: 10-03-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
Compare all formats See Spark UV™ 387 spectral data
Cat # Size Price Quantity Check Availability Save
285510 50 µg SEK2420
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark UV™ 387 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark UV™ 387 has a maximum excitation of 351 nm and a maximum emission of 387 nm.

Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB/199.

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Application References
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
  3. Taylor R, et al. 2007. J. Immunol. 178:5659.
  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer. 

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary. 

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL. 

 

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. 

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services. 

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance. 

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool. 

What if my requested antibody-dye combination doesn't perform as expected?

Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.

Other Formats

View All CD127 Reagents Request Custom Conjugation
Description Clone Applications
PE/Dazzle™ 594 anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 510™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Purified anti-mouse CD127 (IL-7Rα) (Maxpar® Ready) A7R34 FC,CyTOF®
Brilliant Violet 785™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 711™ anti-mouse CD127 (IL-7Rα) A7R34 FC
APC/Cyanine7 anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 650™ anti-mouse CD127 (IL-7Rα) A7R34 FC
TotalSeq™-A0198 anti-mouse CD127 (IL-7Rα) A7R34 PG
TotalSeq™-C0198 anti-mouse CD127 (IL-7Rα) A7R34 PG
Ultra-LEAF™ Purified anti-mouse CD127 (IL-7Rα) A7R34 FC,IP,WB,Block,IHC,ICC
TotalSeq™-B0198 anti-mouse CD127 (IL-7Rα) A7R34 PG
Purified anti-mouse CD127 (IL-7Rα) A7R34 FC,IP,WB,IHC,ICC
FITC anti-mouse CD127 (IL-7Rα) A7R34 FC
PE anti-mouse CD127 (IL-7Rα) A7R34 FC
APC anti-mouse CD127 (IL-7Rα) A7R34 FC
PE/Cyanine7 anti-mouse CD127 (IL-7Rα) A7R34 FC
PE/Cyanine5 anti-mouse CD127 (IL-7Rα) A7R34 FC
Alexa Fluor® 488 anti-mouse CD127 (IL-7Rα) A7R34 FC
Alexa Fluor® 647 anti-mouse CD127 (IL-7Rα) A7R34 FC
PerCP/Cyanine5.5 anti-mouse CD127 (IL-7Rα) A7R34 FC
Biotin anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 421™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 605™ anti-mouse CD127 (IL-7Rα) A7R34 FC
PerCP/Fire™ 780 anti-mouse CD127 (IL-7Rα) A7R34 FC
Brilliant Violet 750™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Spark UV™ 387 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) A7R34 FC
Spark YG™ 581 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) A7R34 FC
Spark YG™ 593 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) A7R34 FC
Spark NIR™ 685 anti-mouse CD127 (IL-7Rα) (Flexi-Fluor™) A7R34 FC
Spark PLUS B488™ anti-mouse CD127 (IL-7Rα) A7R34 FC
Go To Top Version: 1    Revision Date: 10-03-2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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