Loading...

APC anti-mouse CD45.2 Antibody APC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg DKK197
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109814 100 µg DKK690
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  3. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  4. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  5. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  6. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  7. He D, et al. 2022. Immunity. 55:159. PubMed
  8. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  9. Campisi L, et al. 2022. Nature. 606:945. PubMed
  10. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  11. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  12. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  13. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  14. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  15. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  16. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  17. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  18. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  19. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  20. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  21. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  22. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  23. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  24. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  25. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  26. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  27. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  28. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  29. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  30. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  31. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  32. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  33. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  34. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  35. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  36. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  37. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  38. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  39. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  40. Lu SX, et al. 2021. Cell. . PubMed
  41. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  42. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  43. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  44. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  45. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  46. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  47. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  48. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  49. Marangoni F, et al. 2021. Cell. . PubMed
  50. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  51. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  52. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  53. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  56. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  57. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  58. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  59. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  60. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  61. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  62. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  63. Markey K, et al. 2012. Blood. 119:5918. PubMed
  64. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  65. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  66. Bei T, et al. 2021. Molecules. 26:. PubMed
  67. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  68. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  69. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  70. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  71. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  72. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  73. Xu X, et al. 2018. Cell. 175:1336. PubMed
  74. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  75. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  76. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  77. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  78. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  79. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  80. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  81. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  82. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  83. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  84. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  85. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  86. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  87. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  88. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  89. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  90. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  91. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  92. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  93. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  94. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  95. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  96. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  97. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  98. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  99. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  100. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  101. Koide S, et al. 2022. iScience. 25:103603. PubMed
  102. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  103. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  104. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  105. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  106. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  107. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  108. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  109. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  110. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  111. Wang X, et al. 2022. Elife. 11:. PubMed
  112. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  113. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  114. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  115. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  116. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  117. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  118. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  119. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  120. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  121. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  122. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  123. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  124. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  125. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  126. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  127. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  128. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  129. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  130. He D, et al. 2022. Immunity. 55:159. PubMed
  131. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  132. Campisi L, et al. 2022. Nature. 606:945. PubMed
  133. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  134. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  135. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  136. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  137. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  138. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  139. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  140. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  141. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  142. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  143. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  144. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  145. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  146. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  147. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  148. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  149. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  150. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  151. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  152. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  153. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  154. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  155. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  156. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  157. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  158. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  159. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  160. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  161. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  162. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  163. Lu SX, et al. 2021. Cell. . PubMed
  164. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  165. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  166. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  167. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  168. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  169. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  170. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  171. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  172. Marangoni F, et al. 2021. Cell. . PubMed
  173. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  174. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  175. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  176. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  177. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  178. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  179. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  180. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  181. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  182. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  183. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  184. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  185. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  186. Markey K, et al. 2012. Blood. 119:5918. PubMed
  187. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  188. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  189. Bei T, et al. 2021. Molecules. 26:. PubMed
  190. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  191. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  192. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  193. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  194. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  195. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  196. Xu X, et al. 2018. Cell. 175:1336. PubMed
  197. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  198. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  199. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  200. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  201. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  202. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  203. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  204. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  205. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  206. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  207. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  208. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  209. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  210. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  211. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  212. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  213. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  214. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  215. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  216. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  217. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  218. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  219. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  220. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  221. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  222. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  223. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  224. Koide S, et al. 2022. iScience. 25:103603. PubMed
  225. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  226. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  227. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  228. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  229. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  230. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  231. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  232. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  233. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  234. Wang X, et al. 2022. Elife. 11:. PubMed
  235. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  236. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  237. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  238. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  239. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  240. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  241. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  242. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  243. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  244. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  245. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  246. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg DKK197
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109814 100 µg DKK690
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  3. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  4. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  5. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  6. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  7. He D, et al. 2022. Immunity. 55:159. PubMed
  8. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  9. Campisi L, et al. 2022. Nature. 606:945. PubMed
  10. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  11. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  12. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  13. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  14. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  15. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  16. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  17. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  18. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  19. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  20. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  21. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  22. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  23. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  24. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  25. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  26. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  27. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  28. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  29. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  30. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  31. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  32. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  33. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  34. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  35. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  36. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  37. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  38. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  39. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  40. Lu SX, et al. 2021. Cell. . PubMed
  41. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  42. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  43. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  44. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  45. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  46. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  47. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  48. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  49. Marangoni F, et al. 2021. Cell. . PubMed
  50. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  51. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  52. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  53. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  56. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  57. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  58. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  59. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  60. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  61. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  62. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  63. Markey K, et al. 2012. Blood. 119:5918. PubMed
  64. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  65. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  66. Bei T, et al. 2021. Molecules. 26:. PubMed
  67. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  68. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  69. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  70. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  71. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  72. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  73. Xu X, et al. 2018. Cell. 175:1336. PubMed
  74. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  75. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  76. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  77. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  78. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  79. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  80. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  81. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  82. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  83. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  84. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  85. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  86. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  87. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  88. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  89. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  90. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  91. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  92. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  93. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  94. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  95. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  96. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  97. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  98. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  99. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  100. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  101. Koide S, et al. 2022. iScience. 25:103603. PubMed
  102. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  103. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  104. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  105. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  106. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  107. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  108. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  109. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  110. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  111. Wang X, et al. 2022. Elife. 11:. PubMed
  112. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  113. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  114. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  115. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  116. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  117. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  118. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  119. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  120. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  121. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  122. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  123. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  124. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  125. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  126. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  127. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  128. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  129. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  130. He D, et al. 2022. Immunity. 55:159. PubMed
  131. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  132. Campisi L, et al. 2022. Nature. 606:945. PubMed
  133. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  134. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  135. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  136. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  137. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  138. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  139. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  140. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  141. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  142. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  143. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  144. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  145. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  146. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  147. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  148. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  149. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  150. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  151. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  152. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  153. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  154. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  155. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  156. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  157. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  158. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  159. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  160. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  161. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  162. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  163. Lu SX, et al. 2021. Cell. . PubMed
  164. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  165. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  166. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  167. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  168. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  169. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  170. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  171. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  172. Marangoni F, et al. 2021. Cell. . PubMed
  173. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  174. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  175. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  176. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  177. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  178. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  179. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  180. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  181. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  182. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  183. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  184. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  185. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  186. Markey K, et al. 2012. Blood. 119:5918. PubMed
  187. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  188. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  189. Bei T, et al. 2021. Molecules. 26:. PubMed
  190. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  191. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  192. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  193. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  194. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  195. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  196. Xu X, et al. 2018. Cell. 175:1336. PubMed
  197. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  198. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  199. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  200. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  201. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  202. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  203. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  204. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  205. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  206. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  207. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  208. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  209. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  210. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  211. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  212. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  213. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  214. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  215. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  216. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  217. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  218. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  219. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  220. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  221. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  222. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  223. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  224. Koide S, et al. 2022. iScience. 25:103603. PubMed
  225. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  226. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  227. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  228. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  229. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  230. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  231. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  232. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  233. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  234. Wang X, et al. 2022. Elife. 11:. PubMed
  235. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  236. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  237. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  238. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  239. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  240. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  241. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  242. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  243. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  244. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  245. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  246. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account