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FITC anti-mouse CD45.2 Antibody FITC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
109805 50 µg DKK321
Check Availability


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109806 500 µg DKK690
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  4. He D, et al. 2022. Immunity. 55:159. PubMed
  5. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  6. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  7. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  8. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  9. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  10. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  11. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  12. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  13. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  14. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  15. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  16. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  17. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  18. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  19. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  20. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  21. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  22. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  23. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  24. Burman A, et al. 2007. Blood. 110:1064. PubMed
  25. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  26. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  27. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  28. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  29. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  30. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  31. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  32. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  33. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  34. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  35. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  36. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  37. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  38. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  39. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  40. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  41. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  42. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  43. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  44. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  45. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  46. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  47. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  48. Li J, et al. 2020. Elife. 9:00. PubMed
  49. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  50. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  51. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  52. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  53. Wan X, et al. 2018. Nature. 560:107. PubMed
  54. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  55. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  56. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  57. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  58. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  59. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  60. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  61. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  62. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  63. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  64. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  65. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  66. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  67. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  68. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  69. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  70. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  71. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  72. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  73. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  74. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  75. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  76. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  77. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  78. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  79. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  80. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  81. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  82. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  83. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  84. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  85. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  86. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  87. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  88. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  89. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  90. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  91. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  92. Koide S, et al. 2022. iScience. 25:103603. PubMed
  93. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  94. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  95. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  96. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  97. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  98. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  99. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  100. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  101. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  102. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  103. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  104. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  105. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  106. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  107. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  108. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  109. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  110. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  111. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  112. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  113. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  114. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  115. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  116. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  117. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  118. He D, et al. 2022. Immunity. 55:159. PubMed
  119. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  120. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  121. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  122. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  123. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  124. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  125. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  126. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  127. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  128. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  129. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  130. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  131. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  132. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  133. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  134. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  135. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  136. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  137. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  138. Burman A, et al. 2007. Blood. 110:1064. PubMed
  139. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  140. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  141. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  142. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  143. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  144. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  145. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  146. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  147. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  148. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  149. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  150. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  151. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  152. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  153. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  154. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  155. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  156. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  157. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  158. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  159. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  160. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  161. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  162. Li J, et al. 2020. Elife. 9:00. PubMed
  163. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  164. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  165. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  166. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  167. Wan X, et al. 2018. Nature. 560:107. PubMed
  168. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  169. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  170. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  171. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  172. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  173. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  174. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  175. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  176. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  177. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  178. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  179. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  180. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  181. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  182. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  183. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  184. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  185. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  186. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  187. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  188. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  189. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  190. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  191. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  192. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  193. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  194. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  195. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  196. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  197. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  198. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  199. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  200. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  201. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  202. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  203. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  204. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  205. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  206. Koide S, et al. 2022. iScience. 25:103603. PubMed
  207. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  208. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  209. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  210. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  211. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  212. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  213. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  214. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  215. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  216. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  217. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  218. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  219. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  220. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  221. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  222. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  223. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  224. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  225. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  226. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  227. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  228. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
109805 50 µg DKK321
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109806 500 µg DKK690
Check Availability


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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  4. He D, et al. 2022. Immunity. 55:159. PubMed
  5. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  6. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  7. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  8. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  9. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  10. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  11. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  12. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  13. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  14. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  15. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  16. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  17. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  18. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  19. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  20. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  21. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  22. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  23. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  24. Burman A, et al. 2007. Blood. 110:1064. PubMed
  25. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  26. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  27. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  28. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  29. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  30. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  31. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  32. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  33. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  34. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  35. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  36. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  37. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  38. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  39. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  40. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  41. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  42. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  43. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  44. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  45. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  46. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  47. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  48. Li J, et al. 2020. Elife. 9:00. PubMed
  49. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  50. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  51. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  52. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  53. Wan X, et al. 2018. Nature. 560:107. PubMed
  54. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  55. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  56. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  57. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  58. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  59. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  60. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  61. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  62. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  63. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  64. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  65. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  66. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  67. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  68. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  69. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  70. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  71. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  72. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  73. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  74. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  75. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  76. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  77. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  78. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  79. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  80. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  81. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  82. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  83. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  84. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  85. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  86. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  87. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  88. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  89. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  90. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  91. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  92. Koide S, et al. 2022. iScience. 25:103603. PubMed
  93. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  94. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  95. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  96. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  97. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  98. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  99. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  100. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  101. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  102. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  103. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  104. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  105. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  106. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  107. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  108. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  109. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  110. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  111. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  112. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  113. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  114. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  115. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  116. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  117. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  118. He D, et al. 2022. Immunity. 55:159. PubMed
  119. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  120. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  121. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  122. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  123. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  124. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  125. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  126. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  127. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  128. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  129. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  130. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  131. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  132. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  133. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  134. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  135. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  136. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  137. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  138. Burman A, et al. 2007. Blood. 110:1064. PubMed
  139. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  140. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  141. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  142. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  143. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  144. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  145. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  146. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  147. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  148. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  149. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  150. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  151. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  152. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  153. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  154. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  155. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  156. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  157. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  158. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  159. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  160. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  161. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  162. Li J, et al. 2020. Elife. 9:00. PubMed
  163. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  164. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  165. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  166. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  167. Wan X, et al. 2018. Nature. 560:107. PubMed
  168. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  169. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  170. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  171. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  172. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  173. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  174. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  175. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  176. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  177. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  178. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  179. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  180. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  181. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  182. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  183. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  184. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  185. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  186. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  187. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  188. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  189. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  190. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  191. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  192. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  193. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  194. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  195. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  196. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  197. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  198. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  199. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  200. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  201. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  202. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  203. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  204. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  205. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  206. Koide S, et al. 2022. iScience. 25:103603. PubMed
  207. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  208. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  209. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  210. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  211. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  212. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  213. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  214. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  215. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  216. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  217. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  218. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  219. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  220. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  221. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  222. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  223. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  224. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  225. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  226. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  227. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  228. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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