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Pacific Blue™ anti-mouse CD45.2 Antibody Pacific Blue™ anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 104
  • 104_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 104
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
109819 25 µg DKK559
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109820 100 µg DKK1278
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  2. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  3. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  4. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  5. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  6. Vukovic N, et al. 2022. J Biol Chem. 298:102153. PubMed
  7. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  8. Lu Z, et al. 2022. Cell Rep. 41:111482. PubMed
  9. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  10. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  11. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  12. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  13. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  14. Israelow B, et al. 2020. bioRxiv. . PubMed
  15. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  16. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  17. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  18. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  19. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  20. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  21. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  22. Pascal V, et al. 2007. J Immunol. 179:1751. PubMed
  23. Yalamanoglu A, et al. 2021. Front Immunol. 627944:12. PubMed
  24. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  25. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  26. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  27. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  28. Silva D, et al. 2011. Diabetes. 60:2102. PubMed
  29. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  30. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  31. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  32. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  33. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  34. Keller CW, et al. 2020. Autophagy. 1:. PubMed
  35. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  36. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  37. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  38. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  39. Lin G, et al. 2013. PLoS One. 8:e56014. PubMed
  40. Zhu Y, et al. 2015. Neurobiol Dis. 74:114. PubMed
  41. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  42. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  43. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  44. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  45. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  46. Goldman E, et al. 2021. Cells. 10:. PubMed
  47. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  48. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  49. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  50. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  51. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  52. Dewas C, et al. 2015. J Immunol. 194:1372. PubMed
  53. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  54. Chen X, et al. 2019. Nat Commun. 4.421527778. PubMed
  55. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  56. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
  57. Kincaid E, et al. 2007. J Immunol. 179:3187. PubMed
  58. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  59. Pfefferlé M, et al. 2021. Front Immunol. 12:680855. PubMed
  60. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  61. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
  62. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  63. Ma K, et al. 2022. iScience. 25:104347. PubMed
  64. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
  65. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  66. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  67. Reddy Y 2009. Proc Natl Acad Sci U S A. 106:21689. PubMed
  68. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  69. Udden SN et al. 2019. eLife. 8 pii: e40396. PubMed
  70. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  71. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  72. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  73. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  74. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  75. Tullett KM, et al. 2020. Elife. 9:00. PubMed
  76. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  77. Müller A, et al. 2012. Immunity. 37:147. PubMed
  78. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  79. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  80. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  81. Kratky W, et al. 2011. Proc Natl Acad Sci U S A. 108:17414. PubMed
  82. Zlotoff D, et al. 2010. Blood. 115:1897. PubMed
  83. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  84. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  85. Nagalingam G, et al. 2017. J Immunol. 10.4049/jimmunol.1602069. PubMed
  86. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  87. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  88. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  89. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
  90. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  91. Teijaro J, et al. 2014. Proc Natl Acad Sci U S A. 111:3799. PubMed
  92. Knier B, et al. 2018. Nat Immunol. 1.722916667. PubMed
  93. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  94. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  95. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  96. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  97. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  98. Vukovic N, et al. 2022. J Biol Chem. 298:102153. PubMed
  99. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  100. Lu Z, et al. 2022. Cell Rep. 41:111482. PubMed
  101. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  102. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  103. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  104. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  105. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  106. Israelow B, et al. 2020. bioRxiv. . PubMed
  107. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  108. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  109. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  110. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  111. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  112. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  113. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  114. Pascal V, et al. 2007. J Immunol. 179:1751. PubMed
  115. Yalamanoglu A, et al. 2021. Front Immunol. 627944:12. PubMed
  116. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  117. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  118. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  119. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  120. Silva D, et al. 2011. Diabetes. 60:2102. PubMed
  121. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  122. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  123. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  124. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  125. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  126. Keller CW, et al. 2020. Autophagy. 1:. PubMed
  127. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  128. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  129. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  130. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  131. Lin G, et al. 2013. PLoS One. 8:e56014. PubMed
  132. Zhu Y, et al. 2015. Neurobiol Dis. 74:114. PubMed
  133. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  134. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  135. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  136. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  137. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  138. Goldman E, et al. 2021. Cells. 10:. PubMed
  139. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  140. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  141. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  142. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  143. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  144. Dewas C, et al. 2015. J Immunol. 194:1372. PubMed
  145. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  146. Chen X, et al. 2019. Nat Commun. 4.421527778. PubMed
  147. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  148. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
  149. Kincaid E, et al. 2007. J Immunol. 179:3187. PubMed
  150. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  151. Pfefferlé M, et al. 2021. Front Immunol. 12:680855. PubMed
  152. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  153. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
  154. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  155. Ma K, et al. 2022. iScience. 25:104347. PubMed
  156. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
  157. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  158. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  159. Reddy Y 2009. Proc Natl Acad Sci U S A. 106:21689. PubMed
  160. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  161. Udden SN et al. 2019. eLife. 8 pii: e40396. PubMed
  162. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  163. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  164. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  165. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  166. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  167. Tullett KM, et al. 2020. Elife. 9:00. PubMed
  168. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  169. Müller A, et al. 2012. Immunity. 37:147. PubMed
  170. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  171. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  172. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  173. Kratky W, et al. 2011. Proc Natl Acad Sci U S A. 108:17414. PubMed
  174. Zlotoff D, et al. 2010. Blood. 115:1897. PubMed
  175. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  176. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  177. Nagalingam G, et al. 2017. J Immunol. 10.4049/jimmunol.1602069. PubMed
  178. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  179. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  180. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  181. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
  182. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  183. Teijaro J, et al. 2014. Proc Natl Acad Sci U S A. 111:3799. PubMed
  184. Knier B, et al. 2018. Nat Immunol. 1.722916667. PubMed
RRID
AB_492873 (BioLegend Cat. No. 109819)
AB_492872 (BioLegend Cat. No. 109820)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 104
  • 104_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 104
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
109819 25 µg DKK559
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109820 100 µg DKK1278
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  2. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  3. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  4. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  5. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  6. Vukovic N, et al. 2022. J Biol Chem. 298:102153. PubMed
  7. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  8. Lu Z, et al. 2022. Cell Rep. 41:111482. PubMed
  9. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  10. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  11. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  12. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  13. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  14. Israelow B, et al. 2020. bioRxiv. . PubMed
  15. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  16. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  17. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  18. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  19. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  20. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  21. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  22. Pascal V, et al. 2007. J Immunol. 179:1751. PubMed
  23. Yalamanoglu A, et al. 2021. Front Immunol. 627944:12. PubMed
  24. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  25. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  26. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  27. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  28. Silva D, et al. 2011. Diabetes. 60:2102. PubMed
  29. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  30. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  31. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  32. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  33. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  34. Keller CW, et al. 2020. Autophagy. 1:. PubMed
  35. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  36. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  37. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  38. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  39. Lin G, et al. 2013. PLoS One. 8:e56014. PubMed
  40. Zhu Y, et al. 2015. Neurobiol Dis. 74:114. PubMed
  41. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  42. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  43. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  44. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  45. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  46. Goldman E, et al. 2021. Cells. 10:. PubMed
  47. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  48. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  49. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  50. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  51. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  52. Dewas C, et al. 2015. J Immunol. 194:1372. PubMed
  53. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  54. Chen X, et al. 2019. Nat Commun. 4.421527778. PubMed
  55. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  56. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
  57. Kincaid E, et al. 2007. J Immunol. 179:3187. PubMed
  58. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  59. Pfefferlé M, et al. 2021. Front Immunol. 12:680855. PubMed
  60. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  61. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
  62. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  63. Ma K, et al. 2022. iScience. 25:104347. PubMed
  64. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
  65. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  66. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  67. Reddy Y 2009. Proc Natl Acad Sci U S A. 106:21689. PubMed
  68. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  69. Udden SN et al. 2019. eLife. 8 pii: e40396. PubMed
  70. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  71. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  72. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  73. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  74. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  75. Tullett KM, et al. 2020. Elife. 9:00. PubMed
  76. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  77. Müller A, et al. 2012. Immunity. 37:147. PubMed
  78. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  79. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  80. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  81. Kratky W, et al. 2011. Proc Natl Acad Sci U S A. 108:17414. PubMed
  82. Zlotoff D, et al. 2010. Blood. 115:1897. PubMed
  83. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  84. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  85. Nagalingam G, et al. 2017. J Immunol. 10.4049/jimmunol.1602069. PubMed
  86. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  87. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  88. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  89. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
  90. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  91. Teijaro J, et al. 2014. Proc Natl Acad Sci U S A. 111:3799. PubMed
  92. Knier B, et al. 2018. Nat Immunol. 1.722916667. PubMed
  93. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  94. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  95. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  96. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  97. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  98. Vukovic N, et al. 2022. J Biol Chem. 298:102153. PubMed
  99. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  100. Lu Z, et al. 2022. Cell Rep. 41:111482. PubMed
  101. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  102. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  103. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  104. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  105. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  106. Israelow B, et al. 2020. bioRxiv. . PubMed
  107. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  108. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  109. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  110. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  111. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  112. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  113. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  114. Pascal V, et al. 2007. J Immunol. 179:1751. PubMed
  115. Yalamanoglu A, et al. 2021. Front Immunol. 627944:12. PubMed
  116. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  117. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  118. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  119. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  120. Silva D, et al. 2011. Diabetes. 60:2102. PubMed
  121. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  122. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  123. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  124. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  125. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  126. Keller CW, et al. 2020. Autophagy. 1:. PubMed
  127. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  128. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  129. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  130. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  131. Lin G, et al. 2013. PLoS One. 8:e56014. PubMed
  132. Zhu Y, et al. 2015. Neurobiol Dis. 74:114. PubMed
  133. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  134. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  135. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  136. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  137. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  138. Goldman E, et al. 2021. Cells. 10:. PubMed
  139. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  140. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  141. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  142. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  143. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  144. Dewas C, et al. 2015. J Immunol. 194:1372. PubMed
  145. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  146. Chen X, et al. 2019. Nat Commun. 4.421527778. PubMed
  147. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  148. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
  149. Kincaid E, et al. 2007. J Immunol. 179:3187. PubMed
  150. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  151. Pfefferlé M, et al. 2021. Front Immunol. 12:680855. PubMed
  152. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  153. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
  154. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  155. Ma K, et al. 2022. iScience. 25:104347. PubMed
  156. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
  157. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  158. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  159. Reddy Y 2009. Proc Natl Acad Sci U S A. 106:21689. PubMed
  160. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  161. Udden SN et al. 2019. eLife. 8 pii: e40396. PubMed
  162. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  163. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  164. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  165. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  166. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  167. Tullett KM, et al. 2020. Elife. 9:00. PubMed
  168. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  169. Müller A, et al. 2012. Immunity. 37:147. PubMed
  170. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  171. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  172. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  173. Kratky W, et al. 2011. Proc Natl Acad Sci U S A. 108:17414. PubMed
  174. Zlotoff D, et al. 2010. Blood. 115:1897. PubMed
  175. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  176. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  177. Nagalingam G, et al. 2017. J Immunol. 10.4049/jimmunol.1602069. PubMed
  178. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  179. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  180. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  181. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
  182. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  183. Teijaro J, et al. 2014. Proc Natl Acad Sci U S A. 111:3799. PubMed
  184. Knier B, et al. 2018. Nat Immunol. 1.722916667. PubMed
RRID
AB_492873 (BioLegend Cat. No. 109819)
AB_492872 (BioLegend Cat. No. 109820)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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