Alexa Fluor® 700 anti-mouse CD45 Antibody Alexa Fluor® 700 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-F11_Alx700_020608
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 700
  • 30-F11_Alx700_020608
    C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 700
  • 54_Mouse_Liver_Ecadherin_CD45
    Confocal image of C57BL/6 mouse liver sample acquired using the IBEX method of highly multiplexed antibody-based imaging: E-Cadherin (red) in Cycle 1 and CD45 (cyan) in Cycle 3. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
103128 100 µg NOK1774
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103127 25 µg NOK776
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  2. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  3. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  4. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  5. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  6. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  7. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  8. Gu J, et al. 2023. J Cell Biochem. 124:557. PubMed
  9. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
  10. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  11. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  12. Cai X, et al. 2023. Nat Commun. 14:2004. PubMed
  13. Lucas B, et al. 2023. Nat Commun. 14:2066. PubMed
  14. Terzic J, et al. 2023. EMBO Mol Med. 15:e17209. PubMed
  15. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  16. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
  17. Dong X, et al. 2023. J Transl Med. 21:400. PubMed
  18. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  19. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  20. Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed
  21. Yin Q, et al. 2022. J Invest Dermatol. 142:2173. PubMed
  22. Paik D, et al. 2022. Nature. 603:907. PubMed
  23. van Dierendonck XAMH, et al. 2022. Proc Natl Acad Sci U S A. 119:e2114739119. PubMed
  24. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  25. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  26. Shallberg LA, et al. 2022. PLoS Pathog. 18:e1010296. PubMed
  27. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  28. Brown JA, et al. 2022. Gut Microbes. 14:2105609. PubMed
  29. Balood M, et al. 2022. Nature. 611:405. PubMed
  30. Schepers M, et al. 2023. Brain Behav Immun. 109:1. PubMed
  31. He Y, et al. 2023. Nat Commun. 14:691. PubMed
  32. Kraynak CA, et al. 2022. Int J Pharm. 618:121634. PubMed
  33. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  34. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  35. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  36. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  37. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  38. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  39. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  40. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  41. Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed
  42. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  43. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  44. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  45. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  46. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  47. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  48. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  49. Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed
  50. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  51. Ghosh S, et al. 2022. APL Bioeng. 6:036105. PubMed
  52. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  53. Xi H, et al. 2016. J Exp Med. 213: 189 - 207. PubMed
  54. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  55. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  56. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  57. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  58. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  59. Zhang C, et al. 2022. J Extracell Vesicles. 11:e12209. PubMed
  60. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  61. Fujii Y, et al. 2022. JBMR Plus. 6:e10562. PubMed
  62. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  63. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  64. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  65. Egusquiza RJ, et al. 2020. Environ Health Perspect. 128:47011. PubMed
  66. McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
  67. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  68. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  69. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  70. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  71. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  72. van Elsas MJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  73. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  74. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
  75. Yang R, et al. 2012. J Immunol. 189:2656. PubMed
  76. Daubeuf F, et al. 2017. Curr Protoc Mouse Biol. 10.1002/cpmo.26. PubMed
  77. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  78. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  79. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  80. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
  81. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  82. Tanaka S, et al. 2012. J Immunol. 188:6145. PubMed
  83. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  84. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  85. Daubeuf F, et al. 2021. Cells. 10:. PubMed
  86. An J, et al. 2022. iScience. 25:103570. PubMed
  87. Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
  88. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  89. Abu El Maaty MA, et al. 2022. Sci Adv. 8:eabo2295. PubMed
  90. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  91. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  92. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  93. Sun L, et al. 2020. J Immunol. 2177:204. PubMed
  94. Magupalli VG, et al. 2020. Science. :369. PubMed
  95. Li J, et al. 2020. Cancer Discov. . PubMed
  96. Duque-Correa MA, et al. 2022. Nat Commun. 13:1725. PubMed
  97. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  98. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  99. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
  100. Benne N, et al. 2020. Adv Healthc Mater. 9:e2000043. PubMed
  101. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  102. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  103. Levi J, et al. 2020. J Nucl Med. . PubMed
  104. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  105. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  106. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  107. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  108. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  109. Li J, et al. 2018. Immunity. 49:178. PubMed
  110. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  111. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  112. Zhao F, et al. 2022. Nat Commun. 13:6117. PubMed
  113. Coursey T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.83. PubMed
  114. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  115. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  116. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  117. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  118. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  119. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  120. Acker KP, et al. 2019. iScience. 19:281. PubMed
  121. Schmid MC, et al. 2022. Nat Commun. 13:1768. PubMed
  122. Chen X, et al. 2022. Front Immunol. 13:828319. PubMed
  123. He X, et al. 2022. Cancer Immunol Res. 10:314. PubMed
  124. Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
  125. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  126. Abboud D, et al. 2015. Sci Rep. 5: 14746. PubMed
  127. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  128. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  129. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  130. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  131. Leyva‐Castillo JM et al. 2019. Immunity. 50(5):1262-1275 . PubMed
  132. Avraham S, et al. 2019. Oncogene. 38:3812. PubMed
  133. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  134. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  135. Zhang HG, et al. 2022. Cell Res. :. PubMed
  136. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  137. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  138. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  139. Park HB, et al. 2020. Nat Microbiol. 1319:5. PubMed
  140. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  141. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  142. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  143. Crauste F, et al. 2017. Cell Syst. 0.379166667. PubMed
  144. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  145. Ma J, et al. 2021. J Transl Med. 19:477. PubMed
  146. Clark JT, et al. 2021. eLife. 10:00. PubMed
  147. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  148. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  149. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  150. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  151. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  152. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  153. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  154. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  155. Dustin CM, et al. 2021. J Immunol. 206:2989. PubMed
  156. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  157. Bollenbach M, et al. 2021. Molecules. 26:. PubMed
  158. Artham S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L750:318. PubMed
  159. Grajchen E, et al. 2020. J Neuroinflammation. 0.863888889. PubMed
  160. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  161. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  162. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  163. Liu W, et al. 2022. J Clin Invest. 132: . PubMed
  164. Natale CA, et al. 2018. Elife. 7. PubMed
  165. Ayturk UM, et al. 2020. J Bone Miner Res. 35:1981. PubMed
  166. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  167. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  168. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  169. Gicheva N, et al. 2016. Biochem Biophys Res Commun. 479:1-4. PubMed
  170. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  171. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  172. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  173. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  174. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  175. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  176. Melhem NJ, et al. 2021. Circulation. 143:566. PubMed
  177. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  178. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  179. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
  180. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  181. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  182. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  183. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  184. Korin B, et al. 2020. Sleep. :43. PubMed
  185. Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed
  186. Molgora M, et al. 2020. Cell. 182:886. PubMed
  187. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  188. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  189. Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed
  190. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  191. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  192. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  193. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  194. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  195. Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed
  196. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  197. Guey B, et al. 2014. Proc Natl Acad Sci U S A. 111:17254. PubMed
  198. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  199. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  200. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  201. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  202. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  203. Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed
  204. Chen Y, et al. 2022. Stem Cells Int. 2022:1309684. PubMed
  205. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  206. Tian F, et al. 2016. Nat Commun. 7:13283. PubMed
  207. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  208. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  209. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  210. Rossi G, et al. 2022. Sci Rep. 12:13380. PubMed
  211. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  212. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  213. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  214. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
  215. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  216. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  217. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  218. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  219. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  220. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  221. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  222. Gu J, et al. 2023. J Cell Biochem. 124:557. PubMed
  223. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
  224. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  225. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  226. Cai X, et al. 2023. Nat Commun. 14:2004. PubMed
  227. Lucas B, et al. 2023. Nat Commun. 14:2066. PubMed
  228. Terzic J, et al. 2023. EMBO Mol Med. 15:e17209. PubMed
  229. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  230. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
  231. Dong X, et al. 2023. J Transl Med. 21:400. PubMed
  232. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  233. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  234. Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed
  235. Yin Q, et al. 2022. J Invest Dermatol. 142:2173. PubMed
  236. Paik D, et al. 2022. Nature. 603:907. PubMed
  237. van Dierendonck XAMH, et al. 2022. Proc Natl Acad Sci U S A. 119:e2114739119. PubMed
  238. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  239. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  240. Shallberg LA, et al. 2022. PLoS Pathog. 18:e1010296. PubMed
  241. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  242. Brown JA, et al. 2022. Gut Microbes. 14:2105609. PubMed
  243. Balood M, et al. 2022. Nature. 611:405. PubMed
  244. Schepers M, et al. 2023. Brain Behav Immun. 109:1. PubMed
  245. He Y, et al. 2023. Nat Commun. 14:691. PubMed
  246. Kraynak CA, et al. 2022. Int J Pharm. 618:121634. PubMed
  247. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  248. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  249. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  250. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  251. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  252. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  253. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  254. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  255. Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed
  256. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  257. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  258. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  259. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  260. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  261. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  262. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  263. Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed
  264. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  265. Ghosh S, et al. 2022. APL Bioeng. 6:036105. PubMed
  266. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  267. Xi H, et al. 2016. J Exp Med. 213: 189 - 207. PubMed
  268. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  269. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  270. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  271. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  272. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  273. Zhang C, et al. 2022. J Extracell Vesicles. 11:e12209. PubMed
  274. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  275. Fujii Y, et al. 2022. JBMR Plus. 6:e10562. PubMed
  276. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  277. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  278. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  279. Egusquiza RJ, et al. 2020. Environ Health Perspect. 128:47011. PubMed
  280. McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
  281. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  282. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  283. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  284. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  285. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  286. van Elsas MJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  287. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  288. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
  289. Yang R, et al. 2012. J Immunol. 189:2656. PubMed
  290. Daubeuf F, et al. 2017. Curr Protoc Mouse Biol. 10.1002/cpmo.26. PubMed
  291. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  292. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  293. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  294. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
  295. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  296. Tanaka S, et al. 2012. J Immunol. 188:6145. PubMed
  297. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  298. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  299. Daubeuf F, et al. 2021. Cells. 10:. PubMed
  300. An J, et al. 2022. iScience. 25:103570. PubMed
  301. Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
  302. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  303. Abu El Maaty MA, et al. 2022. Sci Adv. 8:eabo2295. PubMed
  304. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  305. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  306. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  307. Sun L, et al. 2020. J Immunol. 2177:204. PubMed
  308. Magupalli VG, et al. 2020. Science. :369. PubMed
  309. Li J, et al. 2020. Cancer Discov. . PubMed
  310. Duque-Correa MA, et al. 2022. Nat Commun. 13:1725. PubMed
  311. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  312. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  313. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
  314. Benne N, et al. 2020. Adv Healthc Mater. 9:e2000043. PubMed
  315. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  316. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  317. Levi J, et al. 2020. J Nucl Med. . PubMed
  318. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  319. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  320. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  321. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  322. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  323. Li J, et al. 2018. Immunity. 49:178. PubMed
  324. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  325. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  326. Zhao F, et al. 2022. Nat Commun. 13:6117. PubMed
  327. Coursey T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.83. PubMed
  328. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  329. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  330. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  331. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  332. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  333. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  334. Acker KP, et al. 2019. iScience. 19:281. PubMed
  335. Schmid MC, et al. 2022. Nat Commun. 13:1768. PubMed
  336. Chen X, et al. 2022. Front Immunol. 13:828319. PubMed
  337. He X, et al. 2022. Cancer Immunol Res. 10:314. PubMed
  338. Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
  339. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  340. Abboud D, et al. 2015. Sci Rep. 5: 14746. PubMed
  341. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  342. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  343. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  344. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  345. Leyva‐Castillo JM et al. 2019. Immunity. 50(5):1262-1275 . PubMed
  346. Avraham S, et al. 2019. Oncogene. 38:3812. PubMed
  347. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  348. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  349. Zhang HG, et al. 2022. Cell Res. :. PubMed
  350. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  351. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  352. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  353. Park HB, et al. 2020. Nat Microbiol. 1319:5. PubMed
  354. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  355. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  356. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  357. Crauste F, et al. 2017. Cell Syst. 0.379166667. PubMed
  358. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  359. Ma J, et al. 2021. J Transl Med. 19:477. PubMed
  360. Clark JT, et al. 2021. eLife. 10:00. PubMed
  361. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  362. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  363. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  364. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  365. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  366. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  367. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  368. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  369. Dustin CM, et al. 2021. J Immunol. 206:2989. PubMed
  370. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  371. Bollenbach M, et al. 2021. Molecules. 26:. PubMed
  372. Artham S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L750:318. PubMed
  373. Grajchen E, et al. 2020. J Neuroinflammation. 0.863888889. PubMed
  374. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  375. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  376. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  377. Liu W, et al. 2022. J Clin Invest. 132: . PubMed
  378. Natale CA, et al. 2018. Elife. 7. PubMed
  379. Ayturk UM, et al. 2020. J Bone Miner Res. 35:1981. PubMed
  380. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  381. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  382. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  383. Gicheva N, et al. 2016. Biochem Biophys Res Commun. 479:1-4. PubMed
  384. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  385. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  386. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  387. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  388. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  389. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  390. Melhem NJ, et al. 2021. Circulation. 143:566. PubMed
  391. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  392. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  393. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
  394. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  395. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  396. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  397. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  398. Korin B, et al. 2020. Sleep. :43. PubMed
  399. Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed
  400. Molgora M, et al. 2020. Cell. 182:886. PubMed
  401. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  402. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  403. Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed
  404. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  405. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  406. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  407. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  408. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  409. Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed
  410. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  411. Guey B, et al. 2014. Proc Natl Acad Sci U S A. 111:17254. PubMed
  412. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  413. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  414. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  415. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  416. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  417. Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed
  418. Chen Y, et al. 2022. Stem Cells Int. 2022:1309684. PubMed
  419. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  420. Tian F, et al. 2016. Nat Commun. 7:13283. PubMed
  421. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  422. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  423. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  424. Rossi G, et al. 2022. Sci Rep. 12:13380. PubMed
  425. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  426. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  427. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  428. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
RRID
AB_493715 (BioLegend Cat. No. 103128)
AB_493714 (BioLegend Cat. No. 103127)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine5 anti-mouse CD45 30-F11 FC
PE/Cyanine7 anti-mouse CD45 30-F11 FC
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 30-F11 FC
Spark YG™ 581 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS V475™ anti-mouse CD45 Antibody 30-F11 FC
StarBright UltraViolet 740 anti-mouse CD45 30-F11 FC
Go To Top Version: 4    Revision Date: 04.20.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
30-F11_Alx700_020608
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 700
  • 30-F11_Alx700_020608
    C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 700
  • 54_Mouse_Liver_Ecadherin_CD45
    Confocal image of C57BL/6 mouse liver sample acquired using the IBEX method of highly multiplexed antibody-based imaging: E-Cadherin (red) in Cycle 1 and CD45 (cyan) in Cycle 3. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
103128 100 µg NOK1774
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103127 25 µg NOK776
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  2. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  3. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  4. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  5. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  6. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  7. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  8. Gu J, et al. 2023. J Cell Biochem. 124:557. PubMed
  9. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
  10. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  11. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  12. Cai X, et al. 2023. Nat Commun. 14:2004. PubMed
  13. Lucas B, et al. 2023. Nat Commun. 14:2066. PubMed
  14. Terzic J, et al. 2023. EMBO Mol Med. 15:e17209. PubMed
  15. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  16. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
  17. Dong X, et al. 2023. J Transl Med. 21:400. PubMed
  18. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  19. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  20. Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed
  21. Yin Q, et al. 2022. J Invest Dermatol. 142:2173. PubMed
  22. Paik D, et al. 2022. Nature. 603:907. PubMed
  23. van Dierendonck XAMH, et al. 2022. Proc Natl Acad Sci U S A. 119:e2114739119. PubMed
  24. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  25. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  26. Shallberg LA, et al. 2022. PLoS Pathog. 18:e1010296. PubMed
  27. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  28. Brown JA, et al. 2022. Gut Microbes. 14:2105609. PubMed
  29. Balood M, et al. 2022. Nature. 611:405. PubMed
  30. Schepers M, et al. 2023. Brain Behav Immun. 109:1. PubMed
  31. He Y, et al. 2023. Nat Commun. 14:691. PubMed
  32. Kraynak CA, et al. 2022. Int J Pharm. 618:121634. PubMed
  33. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  34. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  35. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  36. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  37. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  38. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  39. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  40. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  41. Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed
  42. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  43. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  44. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  45. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  46. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  47. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  48. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  49. Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed
  50. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  51. Ghosh S, et al. 2022. APL Bioeng. 6:036105. PubMed
  52. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  53. Xi H, et al. 2016. J Exp Med. 213: 189 - 207. PubMed
  54. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  55. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  56. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  57. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  58. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  59. Zhang C, et al. 2022. J Extracell Vesicles. 11:e12209. PubMed
  60. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  61. Fujii Y, et al. 2022. JBMR Plus. 6:e10562. PubMed
  62. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  63. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  64. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  65. Egusquiza RJ, et al. 2020. Environ Health Perspect. 128:47011. PubMed
  66. McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
  67. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  68. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  69. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  70. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  71. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  72. van Elsas MJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  73. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  74. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
  75. Yang R, et al. 2012. J Immunol. 189:2656. PubMed
  76. Daubeuf F, et al. 2017. Curr Protoc Mouse Biol. 10.1002/cpmo.26. PubMed
  77. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  78. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  79. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  80. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
  81. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  82. Tanaka S, et al. 2012. J Immunol. 188:6145. PubMed
  83. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  84. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  85. Daubeuf F, et al. 2021. Cells. 10:. PubMed
  86. An J, et al. 2022. iScience. 25:103570. PubMed
  87. Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
  88. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  89. Abu El Maaty MA, et al. 2022. Sci Adv. 8:eabo2295. PubMed
  90. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  91. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  92. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  93. Sun L, et al. 2020. J Immunol. 2177:204. PubMed
  94. Magupalli VG, et al. 2020. Science. :369. PubMed
  95. Li J, et al. 2020. Cancer Discov. . PubMed
  96. Duque-Correa MA, et al. 2022. Nat Commun. 13:1725. PubMed
  97. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  98. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  99. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
  100. Benne N, et al. 2020. Adv Healthc Mater. 9:e2000043. PubMed
  101. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  102. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  103. Levi J, et al. 2020. J Nucl Med. . PubMed
  104. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  105. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  106. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  107. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  108. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  109. Li J, et al. 2018. Immunity. 49:178. PubMed
  110. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  111. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  112. Zhao F, et al. 2022. Nat Commun. 13:6117. PubMed
  113. Coursey T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.83. PubMed
  114. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  115. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  116. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  117. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  118. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  119. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  120. Acker KP, et al. 2019. iScience. 19:281. PubMed
  121. Schmid MC, et al. 2022. Nat Commun. 13:1768. PubMed
  122. Chen X, et al. 2022. Front Immunol. 13:828319. PubMed
  123. He X, et al. 2022. Cancer Immunol Res. 10:314. PubMed
  124. Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
  125. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  126. Abboud D, et al. 2015. Sci Rep. 5: 14746. PubMed
  127. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  128. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  129. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  130. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  131. Leyva‐Castillo JM et al. 2019. Immunity. 50(5):1262-1275 . PubMed
  132. Avraham S, et al. 2019. Oncogene. 38:3812. PubMed
  133. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  134. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  135. Zhang HG, et al. 2022. Cell Res. :. PubMed
  136. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  137. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  138. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  139. Park HB, et al. 2020. Nat Microbiol. 1319:5. PubMed
  140. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  141. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  142. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  143. Crauste F, et al. 2017. Cell Syst. 0.379166667. PubMed
  144. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  145. Ma J, et al. 2021. J Transl Med. 19:477. PubMed
  146. Clark JT, et al. 2021. eLife. 10:00. PubMed
  147. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  148. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  149. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  150. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  151. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  152. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  153. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  154. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  155. Dustin CM, et al. 2021. J Immunol. 206:2989. PubMed
  156. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  157. Bollenbach M, et al. 2021. Molecules. 26:. PubMed
  158. Artham S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L750:318. PubMed
  159. Grajchen E, et al. 2020. J Neuroinflammation. 0.863888889. PubMed
  160. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  161. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  162. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  163. Liu W, et al. 2022. J Clin Invest. 132: . PubMed
  164. Natale CA, et al. 2018. Elife. 7. PubMed
  165. Ayturk UM, et al. 2020. J Bone Miner Res. 35:1981. PubMed
  166. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  167. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  168. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  169. Gicheva N, et al. 2016. Biochem Biophys Res Commun. 479:1-4. PubMed
  170. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  171. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  172. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  173. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  174. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  175. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  176. Melhem NJ, et al. 2021. Circulation. 143:566. PubMed
  177. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  178. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  179. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
  180. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  181. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  182. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  183. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  184. Korin B, et al. 2020. Sleep. :43. PubMed
  185. Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed
  186. Molgora M, et al. 2020. Cell. 182:886. PubMed
  187. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  188. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  189. Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed
  190. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  191. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  192. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  193. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  194. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  195. Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed
  196. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  197. Guey B, et al. 2014. Proc Natl Acad Sci U S A. 111:17254. PubMed
  198. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  199. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  200. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  201. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  202. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  203. Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed
  204. Chen Y, et al. 2022. Stem Cells Int. 2022:1309684. PubMed
  205. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  206. Tian F, et al. 2016. Nat Commun. 7:13283. PubMed
  207. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  208. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  209. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  210. Rossi G, et al. 2022. Sci Rep. 12:13380. PubMed
  211. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  212. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  213. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  214. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
  215. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  216. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  217. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  218. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  219. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  220. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  221. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  222. Gu J, et al. 2023. J Cell Biochem. 124:557. PubMed
  223. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
  224. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  225. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  226. Cai X, et al. 2023. Nat Commun. 14:2004. PubMed
  227. Lucas B, et al. 2023. Nat Commun. 14:2066. PubMed
  228. Terzic J, et al. 2023. EMBO Mol Med. 15:e17209. PubMed
  229. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
  230. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
  231. Dong X, et al. 2023. J Transl Med. 21:400. PubMed
  232. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  233. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  234. Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed
  235. Yin Q, et al. 2022. J Invest Dermatol. 142:2173. PubMed
  236. Paik D, et al. 2022. Nature. 603:907. PubMed
  237. van Dierendonck XAMH, et al. 2022. Proc Natl Acad Sci U S A. 119:e2114739119. PubMed
  238. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  239. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  240. Shallberg LA, et al. 2022. PLoS Pathog. 18:e1010296. PubMed
  241. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  242. Brown JA, et al. 2022. Gut Microbes. 14:2105609. PubMed
  243. Balood M, et al. 2022. Nature. 611:405. PubMed
  244. Schepers M, et al. 2023. Brain Behav Immun. 109:1. PubMed
  245. He Y, et al. 2023. Nat Commun. 14:691. PubMed
  246. Kraynak CA, et al. 2022. Int J Pharm. 618:121634. PubMed
  247. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  248. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
  249. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  250. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  251. Fu R, et al. 2020. Sci Rep. 10:1455. PubMed
  252. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  253. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  254. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  255. Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed
  256. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  257. Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed
  258. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  259. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  260. Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
  261. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  262. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  263. Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed
  264. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  265. Ghosh S, et al. 2022. APL Bioeng. 6:036105. PubMed
  266. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  267. Xi H, et al. 2016. J Exp Med. 213: 189 - 207. PubMed
  268. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  269. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  270. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  271. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  272. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  273. Zhang C, et al. 2022. J Extracell Vesicles. 11:e12209. PubMed
  274. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  275. Fujii Y, et al. 2022. JBMR Plus. 6:e10562. PubMed
  276. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  277. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  278. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  279. Egusquiza RJ, et al. 2020. Environ Health Perspect. 128:47011. PubMed
  280. McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
  281. Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
  282. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  283. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  284. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  285. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  286. van Elsas MJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  287. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  288. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
  289. Yang R, et al. 2012. J Immunol. 189:2656. PubMed
  290. Daubeuf F, et al. 2017. Curr Protoc Mouse Biol. 10.1002/cpmo.26. PubMed
  291. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  292. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  293. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  294. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
  295. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  296. Tanaka S, et al. 2012. J Immunol. 188:6145. PubMed
  297. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  298. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  299. Daubeuf F, et al. 2021. Cells. 10:. PubMed
  300. An J, et al. 2022. iScience. 25:103570. PubMed
  301. Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
  302. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  303. Abu El Maaty MA, et al. 2022. Sci Adv. 8:eabo2295. PubMed
  304. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  305. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  306. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
  307. Sun L, et al. 2020. J Immunol. 2177:204. PubMed
  308. Magupalli VG, et al. 2020. Science. :369. PubMed
  309. Li J, et al. 2020. Cancer Discov. . PubMed
  310. Duque-Correa MA, et al. 2022. Nat Commun. 13:1725. PubMed
  311. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  312. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  313. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
  314. Benne N, et al. 2020. Adv Healthc Mater. 9:e2000043. PubMed
  315. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  316. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  317. Levi J, et al. 2020. J Nucl Med. . PubMed
  318. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  319. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  320. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  321. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  322. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  323. Li J, et al. 2018. Immunity. 49:178. PubMed
  324. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  325. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  326. Zhao F, et al. 2022. Nat Commun. 13:6117. PubMed
  327. Coursey T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.83. PubMed
  328. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  329. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  330. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  331. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  332. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  333. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  334. Acker KP, et al. 2019. iScience. 19:281. PubMed
  335. Schmid MC, et al. 2022. Nat Commun. 13:1768. PubMed
  336. Chen X, et al. 2022. Front Immunol. 13:828319. PubMed
  337. He X, et al. 2022. Cancer Immunol Res. 10:314. PubMed
  338. Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
  339. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  340. Abboud D, et al. 2015. Sci Rep. 5: 14746. PubMed
  341. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  342. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  343. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  344. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  345. Leyva‐Castillo JM et al. 2019. Immunity. 50(5):1262-1275 . PubMed
  346. Avraham S, et al. 2019. Oncogene. 38:3812. PubMed
  347. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  348. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  349. Zhang HG, et al. 2022. Cell Res. :. PubMed
  350. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  351. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  352. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  353. Park HB, et al. 2020. Nat Microbiol. 1319:5. PubMed
  354. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  355. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  356. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  357. Crauste F, et al. 2017. Cell Syst. 0.379166667. PubMed
  358. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  359. Ma J, et al. 2021. J Transl Med. 19:477. PubMed
  360. Clark JT, et al. 2021. eLife. 10:00. PubMed
  361. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  362. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  363. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  364. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  365. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  366. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  367. Stevenson ER, et al. 2022. J Pharmacol Exp Ther. 382:356. PubMed
  368. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  369. Dustin CM, et al. 2021. J Immunol. 206:2989. PubMed
  370. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  371. Bollenbach M, et al. 2021. Molecules. 26:. PubMed
  372. Artham S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L750:318. PubMed
  373. Grajchen E, et al. 2020. J Neuroinflammation. 0.863888889. PubMed
  374. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  375. Abu El Maaty MA, et al. 2021. Sci Adv. 7: . PubMed
  376. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  377. Liu W, et al. 2022. J Clin Invest. 132: . PubMed
  378. Natale CA, et al. 2018. Elife. 7. PubMed
  379. Ayturk UM, et al. 2020. J Bone Miner Res. 35:1981. PubMed
  380. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  381. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  382. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  383. Gicheva N, et al. 2016. Biochem Biophys Res Commun. 479:1-4. PubMed
  384. Aguilar-Pimentel A, et al. 2017. PLoS One. 12(6):e0178563. PubMed
  385. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  386. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  387. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  388. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  389. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  390. Melhem NJ, et al. 2021. Circulation. 143:566. PubMed
  391. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  392. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  393. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
  394. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  395. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  396. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  397. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  398. Korin B, et al. 2020. Sleep. :43. PubMed
  399. Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed
  400. Molgora M, et al. 2020. Cell. 182:886. PubMed
  401. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  402. Hartwig T et al. 2017. Molecular cell. 65(4):730-742 . PubMed
  403. Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed
  404. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  405. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  406. Lee GR, et al. 2021. JCI Insight. 6:. PubMed
  407. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  408. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  409. Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed
  410. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  411. Guey B, et al. 2014. Proc Natl Acad Sci U S A. 111:17254. PubMed
  412. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  413. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  414. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  415. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  416. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  417. Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed
  418. Chen Y, et al. 2022. Stem Cells Int. 2022:1309684. PubMed
  419. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  420. Tian F, et al. 2016. Nat Commun. 7:13283. PubMed
  421. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  422. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  423. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  424. Rossi G, et al. 2022. Sci Rep. 12:13380. PubMed
  425. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  426. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  427. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  428. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
RRID
AB_493715 (BioLegend Cat. No. 103128)
AB_493714 (BioLegend Cat. No. 103127)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine5 anti-mouse CD45 30-F11 FC
PE/Cyanine7 anti-mouse CD45 30-F11 FC
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 30-F11 FC
Spark YG™ 581 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS V475™ anti-mouse CD45 Antibody 30-F11 FC
StarBright UltraViolet 740 anti-mouse CD45 30-F11 FC
Go To Top Version: 4    Revision Date: 04.20.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account