Biotin anti-mouse CD45 Antibody Biotin anti-mouse CD45 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse thymus or spleen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation³, complement-dependent cytotoxicity^1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting⁷, and spatial biology (IBEX)^10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
8. Liu F, et al. 2012. Blood. 119:3295. PubMed
9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Yen W, et al. 2012. Clin Cancer Res. 18:5374. PubMed
2. Dell'Aringa M, et al. 2018. Mucosal Immunol. 1.207638889. PubMed
3. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
4. Tang Y, et al. 2022. Dev Cell. 57:480. PubMed
5. Gbyli R, et al. 2022. Leukemia. 36:1313. PubMed
6. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
7. Matsuzawa M, et al. 2023. Nat Commun. 14:1417. PubMed
8. Papadopoulou D, et al. 2023. JCI Insight. 8:. PubMed
9. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
10. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
11. Tarban N, et al. 2022. Cells. 11:. PubMed
12. Fujimori S, et al. 2022. Elife. 11:. PubMed
13. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
14. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
15. Xie T, et al. 2021. Sci Adv. 7: . PubMed
16. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
17. Kaminitz A, et al. 2013. Int Immunol. 25:485. PubMed
18. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
19. Muhsin-Sharafaldine M, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.03.138. PubMed
20. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
21. Wagner F, et al. 2016. J Bone Joint Surg Am. 98: 916 - 925. PubMed
22. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
23. Ibrahim AM, et al. 2021. Breast Cancer Res. 23:90. PubMed
24. Kurane T, et al. 2020. Okajimas Folia Anat Jpn. 96:49:00. PubMed
25. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
26. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
27. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
28. Wang Z, et al. 2022. Cell Mol Immunol. 19:210. PubMed
29. Shen Z, et al. 2021. iScience. 24:103014. PubMed
30. Lv J, et al. 2021. Cell Discov. 7:24. PubMed
31. Howard E, et al. 2020. J Allergy Clin Immunol. . PubMed
32. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
33. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
34. Nevius E, et al. 2015. J Exp Med. 212: 1931 - 1946. PubMed
35. Watson JK, et al. 2020. Sci Rep. 10:10490. PubMed
36. Zhu H, et al. 2020. J Bone Miner Res. 35:698. PubMed
37. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
38. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
39. Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed
40. Chatterjee S, et al. 2014. PLoS One. 9:87858. PubMed
41. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
42. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
43. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
44. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
45. Pual VM, et al. 2019. PLOS Biology. 17(10):e3000492. PubMed
46. Barnes SE, et al. 2021. J Neuroinflammation. 18:199. PubMed
47. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
48. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
49. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
50. Budai Z, et al. 2021. Cells. 10:. PubMed
51. Kwiecien K, et al. 2020. Sci Rep. 10:13702. PubMed
52. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
53. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
54. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
55. De Micheli AJ, et al. 2020. Cell Rep. 30:3583. PubMed
56. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
57. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
58. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
59. Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed
60. Seong J, et al. 2018. Development. 145:14. PubMed
61. Yen W, et al. 2012. Clin Cancer Res. 18:5374. PubMed
62. Dell'Aringa M, et al. 2018. Mucosal Immunol. 1.207638889. PubMed
63. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
64. Tang Y, et al. 2022. Dev Cell. 57:480. PubMed
65. Gbyli R, et al. 2022. Leukemia. 36:1313. PubMed
66. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
67. Matsuzawa M, et al. 2023. Nat Commun. 14:1417. PubMed
68. Papadopoulou D, et al. 2023. JCI Insight. 8:. PubMed
69. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
70. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
71. Tarban N, et al. 2022. Cells. 11:. PubMed
72. Fujimori S, et al. 2022. Elife. 11:. PubMed
73. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
74. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
75. Xie T, et al. 2021. Sci Adv. 7: . PubMed
76. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
77. Kaminitz A, et al. 2013. Int Immunol. 25:485. PubMed
78. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
79. Muhsin-Sharafaldine M, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.03.138. PubMed
80. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
81. Wagner F, et al. 2016. J Bone Joint Surg Am. 98: 916 - 925. PubMed
82. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
83. Ibrahim AM, et al. 2021. Breast Cancer Res. 23:90. PubMed
84. Kurane T, et al. 2020. Okajimas Folia Anat Jpn. 96:49:00. PubMed
85. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
86. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
87. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
88. Wang Z, et al. 2022. Cell Mol Immunol. 19:210. PubMed
89. Shen Z, et al. 2021. iScience. 24:103014. PubMed
90. Lv J, et al. 2021. Cell Discov. 7:24. PubMed
91. Howard E, et al. 2020. J Allergy Clin Immunol. . PubMed
92. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
93. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
94. Nevius E, et al. 2015. J Exp Med. 212: 1931 - 1946. PubMed
95. Watson JK, et al. 2020. Sci Rep. 10:10490. PubMed
96. Zhu H, et al. 2020. J Bone Miner Res. 35:698. PubMed
97. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
98. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
99. Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed
100. Chatterjee S, et al. 2014. PLoS One. 9:87858. PubMed
101. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
102. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
103. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
104. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
105. Pual VM, et al. 2019. PLOS Biology. 17(10):e3000492. PubMed
106. Barnes SE, et al. 2021. J Neuroinflammation. 18:199. PubMed
107. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
108. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
109. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
110. Budai Z, et al. 2021. Cells. 10:. PubMed
111. Kwiecien K, et al. 2020. Sci Rep. 10:13702. PubMed
112. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
113. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
114. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
115. De Micheli AJ, et al. 2020. Cell Rep. 30:3583. PubMed
116. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
117. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
118. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
119. Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed
120. Seong J, et al. 2018. Development. 145:14. PubMed

RRID

AB_312969 (BioLegend Cat. No. 103104)
AB_312968 (BioLegend Cat. No. 103103)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1

Cell Type

B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

CD Molecules

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?

We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

Certificate of Analysis